##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW185_TGACCA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11701716	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.771747750500865	34.0	31.0	34.0	31.0	34.0
2	32.94401735608692	34.0	31.0	34.0	31.0	34.0
3	33.00893552706287	34.0	33.0	34.0	31.0	34.0
4	36.36203006465035	37.0	37.0	37.0	35.0	37.0
5	36.31072681989548	37.0	37.0	37.0	35.0	37.0
6	36.30559432479817	37.0	37.0	37.0	35.0	37.0
7	36.26321908684162	37.0	37.0	37.0	35.0	37.0
8	36.261807157172505	37.0	37.0	37.0	35.0	37.0
9	38.05394849781006	39.0	38.0	39.0	37.0	39.0
10	37.987041900521255	39.0	38.0	39.0	35.0	39.0
11	38.004800663424064	39.0	38.0	39.0	35.0	39.0
12	37.98385852126304	39.0	38.0	39.0	35.0	39.0
13	37.97479446604242	39.0	38.0	39.0	35.0	39.0
14	39.50830356846808	41.0	39.0	41.0	37.0	41.0
15	39.46423003258668	41.0	39.0	41.0	37.0	41.0
16	39.40574724254118	41.0	39.0	41.0	36.0	41.0
17	39.40003585798869	41.0	39.0	41.0	36.0	41.0
18	39.29891556076049	40.0	39.0	41.0	36.0	41.0
19	39.377878936730305	41.0	39.0	41.0	36.0	41.0
20	39.36565081565815	41.0	39.0	41.0	36.0	41.0
21	39.31781834390785	40.0	39.0	41.0	36.0	41.0
22	39.215847487667624	40.0	39.0	41.0	36.0	41.0
23	39.18713101565616	40.0	39.0	41.0	36.0	41.0
24	39.121996551616874	40.0	39.0	41.0	36.0	41.0
25	39.07306022467132	40.0	39.0	41.0	36.0	41.0
26	38.96016567142802	40.0	39.0	41.0	36.0	41.0
27	38.85892761369358	40.0	38.0	41.0	35.0	41.0
28	38.66962546347903	40.0	38.0	41.0	35.0	41.0
29	38.42471958813562	40.0	38.0	41.0	34.0	41.0
30	38.33085643165498	40.0	38.0	41.0	34.0	41.0
31	38.598335406533536	40.0	38.0	41.0	35.0	41.0
32	38.58945294861027	40.0	38.0	41.0	35.0	41.0
33	38.620180236813134	40.0	38.0	41.0	35.0	41.0
34	38.75616208768013	40.0	38.0	41.0	35.0	41.0
35	38.80981515873398	40.0	38.0	41.0	35.0	41.0
36	38.76009433146386	40.0	38.0	41.0	35.0	41.0
37	38.660437494808455	40.0	38.0	41.0	35.0	41.0
38	38.58794658834653	40.0	38.0	41.0	35.0	41.0
39	38.52695852471552	40.0	38.0	41.0	34.0	41.0
40	38.40603950736798	40.0	38.0	41.0	34.0	41.0
41	38.41838367979534	40.0	38.0	41.0	34.0	41.0
42	38.35964238065596	40.0	38.0	41.0	34.0	41.0
43	38.29004156313484	40.0	38.0	41.0	34.0	41.0
44	38.2056971815074	40.0	38.0	41.0	34.0	41.0
45	38.045316601428375	40.0	37.0	41.0	33.0	41.0
46	37.88041548777974	40.0	37.0	41.0	33.0	41.0
47	37.78393536469352	40.0	37.0	41.0	33.0	41.0
48	37.73605349847834	40.0	37.0	41.0	33.0	41.0
49	37.63589613694265	40.0	36.0	41.0	33.0	41.0
50	37.53124738286248	40.0	36.0	41.0	33.0	41.0
51	36.57761271936526	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	11.0
12	11.0
13	9.0
14	16.0
15	30.0
16	96.0
17	236.0
18	559.0
19	1243.0
20	2407.0
21	4040.0
22	6569.0
23	10557.0
24	15556.0
25	22584.0
26	31865.0
27	43957.0
28	59484.0
29	78293.0
30	101499.0
31	130889.0
32	168310.0
33	219279.0
34	296533.0
35	427849.0
36	683655.0
37	1182341.0
38	2368443.0
39	5842085.0
40	3298.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.598841878181506	24.478389070008397	27.948847439580593	22.973921612229503
2	20.095018542579567	31.732200644760134	27.195908702621054	20.976872110039245
3	20.505539529416026	27.185790528500263	32.6950594254723	19.61361051661141
4	19.632556455822378	26.479817148185784	28.108484259915382	25.779142136076455
5	24.88958884320898	26.876246184747604	28.45648450193117	19.777680470112248
6	25.0987291094742	27.420653517825933	26.968685618416995	20.511931754282877
7	20.474791902315864	32.33586424418436	27.566820114246493	19.622523739253285
8	19.96316608606806	32.715253044938024	27.24628592934575	20.075294939648167
9	24.814121279306388	27.210641584533413	27.588261413966976	20.386975722193224
10	19.93120496173382	32.40715293380902	27.171989133901388	20.48965297055577
11	24.949639864785645	27.264249106712214	27.24133793710256	20.544773091399588
12	19.953184644029985	27.288219949962894	27.180005052250454	25.578590353756663
13	19.82605799012726	32.44718125102335	27.250148610682402	20.476612148166986
14	19.78479908416851	27.47024453507503	27.349612655101186	25.395343725655277
15	19.77758646680538	32.43293547715565	27.43481383414193	20.35466422189703
16	19.790020540577125	27.38255654127993	27.459203419395923	25.36821949874702
17	24.86166986106995	27.32425739951303	27.42565278460014	20.388419954816882
18	19.96360191958171	27.257036489349083	32.370611284703884	20.408750306365324
19	19.939312168109414	27.203837244419997	27.35259567088794	25.504254916582646
20	19.96704072582871	27.22181553915948	32.32357242708205	20.487571307929763
21	25.03254831350891	27.197082741218836	27.2761576434927	20.49421130177955
22	20.02104478387436	32.267663056482675	27.23542503866101	20.475867120981956
23	19.96238842234763	32.340803690672374	27.245465536849466	20.451342350130528
24	20.03274082456594	27.261564851094285	27.316642793860908	25.389051530478866
25	19.98881189733198	27.249832417741125	32.2687886118583	20.4925670730686
26	19.967295137858915	27.310578495261577	27.281488309012538	25.440638057866977
27	20.03018665695693	27.346489847208495	27.24212327079495	25.38120022503963
28	20.117645438186862	32.273025921474506	27.167370652948524	20.441957987390108
29	25.054427158140737	27.33302708285751	27.19801984799122	20.414525911010543
30	20.17922595561376	27.246937104390334	32.09642305410469	20.477413885891217
31	20.100160910914955	27.27619957193576	27.150218370550235	25.47342114659905
32	20.07701314596691	32.21059215246224	27.167861022116146	20.544533679454705
33	20.112759687539906	27.248744992062342	27.27700158103564	25.36149373936211
34	20.139055372156875	27.282416161875155	32.16270418683162	20.415824279136352
35	24.999138875861625	27.407747963366624	27.24891650070262	20.34419666006913
36	20.103093268198162	32.261879229179456	27.211820413137282	20.423207089485096
37	20.096482745773546	27.343725488301878	27.20423936591435	25.35555240001022
38	20.0735843033827	27.413810859282627	27.186451599945816	25.32615323738886
39	20.11854245785642	32.25399474168378	27.202132523209727	20.42533027725007
40	20.162878160386626	32.25522331082335	27.189526862826856	20.39237166596318
41	20.09348114800552	27.343175964227324	32.068761838878515	20.494581048888634
42	20.1820172359018	27.24970450554155	27.218336665133602	25.349941593423043
43	20.14107727197044	27.22525865420153	32.08506176895849	20.548602304869547
44	20.200240171966545	27.26344129675768	27.193124756943405	25.343193774332367
45	25.06986243257259	27.299468342037308	27.250421679069515	20.38024754632059
46	20.177214501755376	27.408108419047867	32.07464357005525	20.340033509141506
47	20.1533757012413	32.25980883162346	27.29079848350694	20.296016983628302
48	20.28348874744713	27.323622868361287	32.057750228007095	20.335138156184495
49	25.11676503920372	27.2792686074517	27.15661489074751	20.44735146259707
50	20.249236520422002	27.279120407252925	27.079642644292747	25.392000428032325
51	20.33109156751164	27.20930624115365	27.103976949944396	25.35562524139031
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.57276349041101
2	41.07189065261881
3	40.119150046027436
4	45.41169859189883
5	44.66726931332123
6	45.610660863757076
7	40.09731564156915
8	40.038461025716224
9	45.201097001499605
10	40.420857932289586
11	45.49441295618523
12	45.53177499778665
13	40.302670138294246
14	45.18014280982379
15	40.132250688702406
16	45.15824003932415
17	45.25008981588684
18	40.37235222594703
19	45.44356708469206
20	40.45461203375847
21	45.52675961528846
22	40.49691190485631
23	40.413730772478154
24	45.42179235504481
25	40.481378970400584
26	45.40793319572589
27	45.411386881996556
28	40.55960342557697
29	45.468953069151276
30	40.656639841504976
31	45.573582057514
32	40.62154682542161
33	45.47425342690202
34	40.55487965129323
35	45.343335535930755
36	40.52630035768326
37	45.45203514578377
38	45.399737540771554
39	40.54387273510649
40	40.5552498263498
41	40.58806219689416
42	45.53195882932485
43	40.689679576839985
44	45.54343394629892
45	45.45010997889318
46	40.517248010896886
47	40.44939268486959
48	40.61862690363162
49	45.56411650180079
50	45.64123694845433
51	45.68671680890195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	60.5
2	75.0
3	194.0
4	313.0
5	627.5
6	942.0
7	1621.0
8	2300.0
9	4088.5
10	5877.0
11	9630.5
12	13384.0
13	20832.0
14	28280.0
15	40393.0
16	52506.0
17	70529.5
18	88553.0
19	114690.5
20	140828.0
21	172857.5
22	204887.0
23	242539.5
24	280192.0
25	316289.5
26	385677.5
27	418968.0
28	450177.0
29	481386.0
30	505787.0
31	530188.0
32	546233.0
33	562278.0
34	580713.0
35	599148.0
36	612079.0
37	625010.0
38	632067.0
39	639124.0
40	639399.5
41	639675.0
42	636991.0
43	634307.0
44	620305.5
45	606304.0
46	586001.0
47	565698.0
48	545784.5
49	525871.0
50	524368.5
51	522866.0
52	741746.5
53	960627.0
54	675342.5
55	390058.0
56	359140.0
57	328222.0
58	309304.0
59	290386.0
60	271163.5
61	251941.0
62	235113.0
63	218285.0
64	202169.5
65	186054.0
66	171262.5
67	156471.0
68	141894.0
69	127317.0
70	111906.5
71	96496.0
72	83004.5
73	69513.0
74	57620.5
75	36878.5
76	28029.0
77	22054.5
78	16080.0
79	12178.0
80	8276.0
81	6118.5
82	3961.0
83	2835.5
84	1710.0
85	1202.0
86	694.0
87	489.5
88	285.0
89	198.5
90	112.0
91	80.0
92	48.0
93	34.5
94	21.0
95	13.5
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042891145196140464
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.001657876502899233
20	1.709151033916735E-5
21	0.012109335075300067
22	3.41830206783347E-5
23	0.0
24	9.9985335484129E-4
25	0.0
26	0.08962788021859358
27	0.1120177587629028
28	0.12156336728732778
29	0.6973165303276887
30	0.1349972944139133
31	0.03366172961299009
32	0.1205036936462994
33	0.015296901753554778
34	0.011459857682411707
35	0.016177114536021896
36	0.028859015207684066
37	0.03841316948727862
38	0.007272437649315708
39	0.015151623915671856
40	0.024868147543488494
41	0.011870053930551724
42	0.015681460736186044
43	0.019757785952077455
44	0.01509180362948477
45	0.022671888464905487
46	0.018988667986814926
47	0.008853402355688687
48	0.030892904938044988
49	0.011075298699780443
50	0.014228682357356819
51	0.010827471799862515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.1701716E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	39.279533782962126
#Duplication Level	Relative count
1	100.0
2	33.4475905841755
3	8.68560512694109
4	2.36705077643579
5	1.0306260783830417
6	0.7163544491003204
7	0.5399617944293813
8	0.4898940103524772
9	0.45215060389450334
10++	6.325486812915948
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	515057	4.401551020380259	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4354955	4.378118	4.8787317	2
AAAAA	4329750	4.1300645	4.3429365	3
ATTTT	3034805	3.019066	3.2243395	3
GGAAG	1157490	2.734701	67.2688	5
GAGCA	1161825	2.6354713	64.34748	9
CGGAA	1159220	2.6295621	64.47049	4
GAAGA	1497275	2.614536	50.250942	6
TCCAG	1175355	2.5868635	61.040836	25
GATCG	1116435	2.5592513	64.76696	1
CTGAA	1463180	2.479007	47.941856	19
TCTCG	1088665	2.421368	61.21956	41
ATCGG	1054230	2.4166563	64.80741	2
CTCCA	1135850	2.4002168	58.338295	24
TCGGA	1007350	2.3091915	64.82679	3
AGAGC	987350	2.239694	64.067924	8
CACTG	1014935	2.2337923	60.65457	31
CCAGT	1010545	2.2241302	60.532	26
GCACA	1020365	2.2222767	61.290928	11
AGCAC	1015320	2.211289	61.36352	10
ATGCC	1003050	2.207634	60.233738	47
GTCTG	950340	2.201509	64.386086	17
CTGAC	982270	2.1618989	60.228035	33
CACAC	1031915	2.1578014	58.497074	12
GACCA	980345	2.135116	58.92142	35
CGTCT	954095	2.122062	61.596962	16
CTCGT	946845	2.1059368	60.629433	42
TGACC	949380	2.0895107	59.605076	34
TCTGA	1219345	2.0877023	48.046944	18
GTCAC	946575	2.083337	60.688576	29
AAGAG	1189315	2.0767775	49.711494	7
ACGTC	933245	2.053999	61.01377	15
TGAAC	1195605	2.0256653	47.46556	20
CACGT	919510	2.0237694	61.258736	14
CAGTC	892035	1.9632989	60.315018	27
ACACG	895420	1.950156	60.662075	13
ACCAA	1188080	1.912444	43.70382	36
CCAAT	1165905	1.8965667	44.152287	37
ACTCC	894700	1.8906317	58.11729	23
ATCTC	1143145	1.8791795	45.24989	40
ACTGA	1107400	1.8762232	46.879757	32
GAACT	1099415	1.8626945	47.282185	21
CAATC	1129760	1.83777	44.149437	38
TCACT	1106160	1.8183812	45.46879	30
AACTC	1098850	1.787489	45.30146	22
AGTCA	995005	1.6857969	46.73383	28
AATCT	1277035	1.6160144	34.825565	39
GTATG	875830	1.5618409	48.529034	45
TATGC	902725	1.545601	46.573563	46
TCGTA	888470	1.5211943	46.622417	43
CGTAT	870450	1.4903413	46.56971	44
>>END_MODULE