##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW183_CGATGT_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5270968	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79911469771776	34.0	31.0	34.0	31.0	34.0
2	32.96964959756917	34.0	31.0	34.0	31.0	34.0
3	33.035470904016115	34.0	33.0	34.0	31.0	34.0
4	36.38095734976953	37.0	37.0	37.0	35.0	37.0
5	36.32657549808688	37.0	37.0	37.0	35.0	37.0
6	36.32522128762687	37.0	37.0	37.0	35.0	37.0
7	36.28015119803421	37.0	37.0	37.0	35.0	37.0
8	36.2851110080729	37.0	37.0	37.0	35.0	37.0
9	38.077221110050374	39.0	38.0	39.0	37.0	39.0
10	38.01705360381622	39.0	38.0	39.0	35.0	39.0
11	38.03313053693363	39.0	38.0	39.0	35.0	39.0
12	37.9998947062475	39.0	38.0	39.0	35.0	39.0
13	38.00157428388865	39.0	38.0	39.0	35.0	39.0
14	39.568169451986805	41.0	40.0	41.0	37.0	41.0
15	39.50675587482223	41.0	39.0	41.0	37.0	41.0
16	39.511674515952286	41.0	39.0	41.0	37.0	41.0
17	39.48115697154678	41.0	39.0	41.0	37.0	41.0
18	39.38763847551342	41.0	39.0	41.0	37.0	41.0
19	39.47836203900308	41.0	39.0	41.0	37.0	41.0
20	39.46064916349331	41.0	39.0	41.0	37.0	41.0
21	39.43359891389969	41.0	39.0	41.0	37.0	41.0
22	39.34411800640793	41.0	39.0	41.0	36.0	41.0
23	39.323654402758656	40.0	39.0	41.0	36.0	41.0
24	39.27051330988919	40.0	39.0	41.0	36.0	41.0
25	39.24554104672993	40.0	39.0	41.0	36.0	41.0
26	39.14539435640664	40.0	39.0	41.0	36.0	41.0
27	39.05603657620384	40.0	39.0	41.0	36.0	41.0
28	38.8654482440417	40.0	38.0	41.0	35.0	41.0
29	38.64404792440402	40.0	38.0	41.0	35.0	41.0
30	38.58918153174142	40.0	38.0	41.0	35.0	41.0
31	38.854828373080615	40.0	38.0	41.0	35.0	41.0
32	38.86468880099443	40.0	38.0	41.0	35.0	41.0
33	38.91635995513538	40.0	38.0	41.0	35.0	41.0
34	39.03158926405928	40.0	39.0	41.0	36.0	41.0
35	39.07305394379173	40.0	39.0	41.0	36.0	41.0
36	38.941640510813194	40.0	39.0	41.0	35.0	41.0
37	38.94356956824629	40.0	39.0	41.0	35.0	41.0
38	38.92830899371804	40.0	39.0	41.0	35.0	41.0
39	38.858636591988414	40.0	39.0	41.0	35.0	41.0
40	38.833648202758965	40.0	38.0	41.0	35.0	41.0
41	38.859914345903825	40.0	38.0	41.0	35.0	41.0
42	38.81468394420152	40.0	38.0	41.0	35.0	41.0
43	38.78048377451732	40.0	38.0	41.0	35.0	41.0
44	38.72840301819324	40.0	38.0	41.0	35.0	41.0
45	38.59560634782833	40.0	38.0	41.0	35.0	41.0
46	38.43717131274559	40.0	38.0	41.0	34.0	41.0
47	38.37533162789074	40.0	38.0	41.0	34.0	41.0
48	38.37179356808844	40.0	38.0	41.0	34.0	41.0
49	38.30294530340537	40.0	38.0	41.0	34.0	41.0
50	38.23371551487317	40.0	38.0	41.0	34.0	41.0
51	37.2989600771623	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	4.0
14	8.0
15	13.0
16	26.0
17	70.0
18	159.0
19	326.0
20	620.0
21	1144.0
22	1929.0
23	3198.0
24	4944.0
25	7659.0
26	11192.0
27	15863.0
28	22402.0
29	30148.0
30	39814.0
31	51958.0
32	66660.0
33	87266.0
34	116981.0
35	165298.0
36	252873.0
37	437524.0
38	998625.0
39	2952450.0
40	1807.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.84665079698281	25.35229445488547	31.863027748917865	19.938026999213854
2	17.680168045034613	34.089431011533364	31.113241438764188	17.117159504667832
3	17.240059131453652	29.298944709965983	36.64721546402862	16.813780694551742
4	16.923134422367962	28.765209729977492	30.584515026461933	23.727140821192616
5	23.490561885407008	29.10374337313374	30.50092506727417	16.904769674185083
6	23.477642057398185	29.597371867937728	29.341593422688206	17.583392651975878
7	17.556926924997455	35.78346520031994	29.90832044512507	16.751287429557532
8	16.977299046399068	36.03193948436037	29.894091559652804	17.096669909587764
9	23.093424205952303	29.27676662047654	30.29530818627622	17.334500987294934
10	17.073562199580795	35.61704415583627	29.8185077200241	17.49088592455883
11	23.321446838607255	29.356998562692848	29.795912249894137	17.52564234880576
12	17.019587294022653	29.51025314515284	29.703955706048678	23.76620385477582
13	16.94339635528047	35.876597998697775	29.730440404874397	17.449565241147358
14	16.943775792226397	29.703841874964905	29.807940401080028	23.54444193172867
15	16.84286453645706	35.99710717272425	29.90158543933486	17.258442851483828
16	16.97500345287621	29.602949591042858	29.942014445923405	23.48003251015753
17	23.24796887402845	29.54055118528513	29.904791681527946	17.30668825915847
18	17.08200467162768	29.436338828086228	36.08796334942652	17.393693150859576
19	17.08346147402938	29.360285598339107	29.87951391214288	23.67673901548863
20	17.166941631973483	29.321255602386508	35.990258335850264	17.521544429789746
21	23.43908287277932	29.33034146363677	29.781303964132476	17.449271699451433
22	17.202235795108525	35.6391978244595	29.743637124580204	17.414929255851774
23	17.19166194900064	35.7122638574167	29.702437958264973	17.393636235317686
24	17.222769671855275	29.422797522854705	29.723846336853665	23.630586468436356
25	17.139090960142426	29.42975559707439	35.87477670135732	17.556376741425865
26	17.20671006357622	29.411919971440074	29.69180345301664	23.689566511967065
27	17.249873313666246	29.485721861388814	29.618979929997728	23.645424894947208
28	17.29382008758841	35.67699612206151	29.567310203736614	17.461873586613468
29	23.47522084009891	29.50853967269603	29.58591514260872	17.430324344596336
30	17.35966528608385	29.41410816192886	35.67779739002025	17.548429161967043
31	17.26173045641938	29.411063629829968	29.554500664330956	23.772705249419694
32	17.314117459564283	35.55366573672131	29.53180255475056	17.600414248963855
33	17.29758283994533	29.459300636618053	29.500760218838284	23.74235630459833
34	17.378799697251836	29.4363582788133	29.528476751327748	23.656365272607115
35	23.47480163982175	29.53514032506117	29.54396371216024	17.44609432295684
36	17.359909349396705	35.58641554177342	29.587701820849677	17.465973287980198
37	17.31060235818842	29.575364760029416	35.669986477189155	17.44404640459301
38	23.471370269249817	29.5192360598569	29.59082224503232	17.41857142586096
39	17.35390112787398	29.476501850695453	35.68061514395806	17.48898187747252
40	17.305546488944216	35.628354119066955	29.615969971383244	17.45012942060558
41	17.349831575020698	29.42537036323746	35.6658701906666	17.558927871075248
42	17.363577972860277	29.36251158651534	29.64165054595376	23.63225989467063
43	17.331087613619825	29.354480189955094	35.66225549476771	17.652176701657364
44	17.428699481116126	29.37705093471581	29.57873369261415	23.615515891553915
45	23.46272243515712	29.51294732299784	29.543764794378774	17.48056544746627
46	17.36076388803236	29.655082640621316	35.586710175945406	17.39744329540092
47	17.334242796237938	35.65398794424069	29.595579934389647	17.41618932513172
48	17.477784953529785	29.490411324943462	35.63714193698618	17.39466178454057
49	23.518432502954255	29.404420643431838	29.50708907392355	17.57005777969036
50	17.44023549649759	29.365736997666325	29.440363764217363	23.75366374161872
51	17.449804633727847	29.3412381741882	29.452407113977287	23.756550078106663
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.78467779619667
2	34.797327549702445
3	34.053839826005394
4	40.65027524356057
5	40.39533155959209
6	41.06103470937407
7	34.30821435455499
8	34.073968955986835
9	40.42792519324724
10	34.56444812413962
11	40.84708918741302
12	40.785791148798474
13	34.39296159642783
14	40.48821772395507
15	34.101307387940885
16	40.45503596303374
17	40.55465713318692
18	34.475697822487255
19	40.76020048951801
20	34.68848606176323
21	40.88835457223075
22	34.617165050960296
23	34.585298184318326
24	40.85335614029163
25	34.69546770156829
26	40.89627657554328
27	40.895298208613454
28	34.75569367420188
29	40.90554518469525
30	34.90809444805089
31	41.03443570583907
32	34.91453170852814
33	41.039939144543666
34	41.035164969858954
35	40.92089596277859
36	34.82588263737691
37	34.75464876278143
38	40.88994169511078
39	34.8428830053465
40	34.7556759095498
41	34.90875944609594
42	40.99583786753091
43	34.98326431527718
44	41.04421537267004
45	40.94328788262339
46	34.758207183433285
47	34.750432121369656
48	34.87244673807036
49	41.088490282644614
50	41.19389923811631
51	41.206354711834514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	66.5
2	62.0
3	169.0
4	276.0
5	547.0
6	818.0
7	1572.0
8	2326.0
9	4086.5
10	5847.0
11	9406.0
12	12965.0
13	18956.0
14	24947.0
15	34420.5
16	43894.0
17	57336.0
18	70778.0
19	89252.0
20	107726.0
21	128702.5
22	149679.0
23	172640.5
24	195602.0
25	216041.0
26	256294.5
27	276109.0
28	290076.0
29	304043.0
30	314947.5
31	325852.0
32	329600.0
33	333348.0
34	337005.5
35	340663.0
36	339017.0
37	337371.0
38	331245.5
39	325120.0
40	318206.5
41	311293.0
42	299556.0
43	287819.0
44	273873.5
45	259928.0
46	240780.0
47	221632.0
48	203869.5
49	186107.0
50	176384.5
51	166662.0
52	295254.5
53	423847.0
54	259893.5
55	95940.0
56	80626.5
57	65313.0
58	57011.0
59	48709.0
60	41874.0
61	35039.0
62	29774.5
63	24510.0
64	20535.0
65	16560.0
66	14060.5
67	11561.0
68	9785.0
69	8009.0
70	6658.0
71	5307.0
72	4405.5
73	3504.0
74	2916.0
75	1847.0
76	1366.0
77	1081.0
78	796.0
79	593.0
80	390.0
81	304.5
82	219.0
83	151.5
84	84.0
85	58.5
86	33.0
87	28.0
88	23.0
89	17.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04215544469251189
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.001309057463448839
20	0.0
21	0.012995715398006591
22	3.794369459271997E-5
23	0.0
24	0.0011762545323743192
25	0.0
26	0.09197551569275321
27	0.11375519638897448
28	0.12470195227897418
29	0.6972343599885258
30	0.13752692105131353
31	0.0333335356997045
32	0.12333597927363628
33	0.014703181654678989
34	0.011022643279185153
35	0.016752141162685866
36	0.02855263018102178
37	0.03970807639128145
38	0.007550795223951274
39	0.014930843822235309
40	0.02519461320956606
41	0.01269216584126483
42	0.01580354879786787
43	0.020698285400328746
44	0.015367196310051586
45	0.02333537217452278
46	0.018611382197729145
47	0.008689106061732874
48	0.031853731610588416
49	0.011458995767001432
50	0.01405813884660275
51	0.011591798698075952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5270968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	45.63195953834581
#Duplication Level	Relative count
1	100.0
2	46.88961507448118
3	14.183253405763272
4	4.116623519925307
5	1.3079828544752365
6	0.613673980392989
7	0.4303356957942537
8	0.31574926792004415
9	0.22832406739379535
10++	1.672113058608836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	298394	5.661085402150041	TruSeq Adapter, Index 2 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACCG	437750	4.2715435	148.14137	31
AAAAA	2780015	3.9772005	4.139274	13
TTTTT	2858770	3.9589434	4.371302	1
CGGAA	572505	3.5959837	98.88306	4
GGAAG	554625	3.5954013	102.026924	5
TCCAG	589480	3.5642815	93.1568	25
GAGCA	554705	3.4841797	98.28294	9
CTCCA	572045	3.3513792	90.00893	24
TCTCG	534390	3.2102225	92.00898	41
GAAAA	1355405	3.2087703	3.4645214	2
TCGGA	507205	3.165162	97.8696	3
TTTTC	1412875	3.1576178	3.6917932	1
AGAGC	489320	3.0734875	97.96002	8
TCACC	521270	3.053909	89.56901	30
GCACA	500285	3.0447133	94.70454	11
CACAC	511820	3.0181215	91.624626	12
CGATG	481515	3.0048459	94.820244	34
ATCGG	481205	3.0029113	97.515076	2
GTCTG	469690	2.9120421	95.59857	17
CTGAA	769660	2.9024992	59.321648	19
CCGAT	479745	2.9007702	91.91176	33
AGCAC	476060	2.897281	94.55452	10
CCAGT	478490	2.8931818	92.33638	26
GAAGA	733790	2.8746204	62.212414	6
ACCGA	463830	2.8228498	92.54333	32
CTCGT	466175	2.800437	91.33479	42
CGTCT	465470	2.7962017	92.6134	16
GTCAC	462260	2.7950478	92.310486	29
CACGT	458930	2.7749126	93.50831	14
GATCG	442810	2.7633114	97.15075	1
ATGCC	454595	2.748701	91.931496	47
ACACG	449835	2.7376769	94.1302	13
ACTCC	458990	2.6890361	89.82978	23
ACGTC	442095	2.6731203	93.24217	15
CAGTC	441990	2.6724854	91.98318	27
TCTGA	660520	2.47476	58.471535	18
AAGAG	592030	2.319276	61.65328	7
TGAAC	596320	2.248809	58.60191	20
GAACT	571050	2.153512	58.42748	21
AACTC	569145	2.0796325	56.552402	22
ATCTC	556145	2.0189505	55.657295	40
GATGT	520765	2.013717	58.850807	35
AGTCA	514165	1.9389907	57.70794	28
GTATG	454205	1.7563399	58.79101	45
TATGC	466405	1.7474723	56.903652	46
TCGTA	465540	1.7442316	56.99725	43
GTATC	451095	1.6901107	56.69265	38
CGTAT	444830	1.6666377	56.91826	44
TGTAT	571225	1.3261681	35.506054	37
ATGTA	554415	1.2955446	35.66546	36
TATCT	539030	1.2125365	34.24937	39
>>END_MODULE