FastQCFastQC Report
Wed 23 Jul 2014
LW182_ATCACG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW182_ATCACG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10329059
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC 394187 3.816291493736264 TruSeq Adapter, Index 1 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 4909240 3.9012132 4.262089 1
AAAAA 4907455 3.784567 3.9694939 3
TTTTC 2504095 3.0471182 3.4120917 2
GAAAA 2442435 3.0041175 3.2499301 2
GGAAG 930930 2.9125872 65.70619 5
CGGAA 936170 2.8290513 63.458767 4
GAGCA 929085 2.8076406 63.270065 9
CTCCA 954380 2.706829 58.329662 24
TCCAG 910625 2.67396 60.284466 25
GAAGA 1270905 2.4931076 41.78845 6
TCGGA 813220 2.4722893 63.445213 3
TCTCG 830710 2.4539738 60.146343 41
CTGAA 1284240 2.4479547 40.214348 19
AGAGC 783640 2.3681145 62.905445 8
GCACA 803980 2.3466887 60.6599 11
ATCGG 765710 2.3278527 63.109077 2
CACAC 825290 2.3267033 58.503616 12
AGCAC 779735 2.2759216 60.660587 10
CCAGT 754170 2.2145455 59.646564 26
GTCTG 714485 2.1851912 62.732986 17
CACGA 739595 2.1587594 58.58095 36
GTCAC 732740 2.1516182 59.53131 29
GATCG 705510 2.1448371 62.80938 1
CTCGT 725615 2.143516 59.649963 42
CGTCT 721725 2.1320248 60.58786 16
CACGT 721200 2.1177323 60.40724 14
ATGCC 712210 2.091334 59.24627 47
TCTGA 1074380 2.0602512 40.047943 18
TCACG 697680 2.0486681 58.95838 35
ACACG 696770 2.0337598 60.130936 13
ACTCC 714675 2.0269735 57.91584 23
CGATC 687365 2.0183792 58.821728 38
ACGTC 686450 2.0156922 60.235157 15
CAGTC 678170 1.9913789 59.32793 27
AAGAG 976480 1.9155402 41.235855 7
CATCA 1028405 1.8934137 37.810307 33
TGAAC 987935 1.8831528 39.6218 20
TCACA 989755 1.8222545 37.78985 30
GAACT 902190 1.71971 39.33896 21
AACTC 924745 1.7025635 38.034824 22
CACAT 911315 1.6778374 37.647266 31
ATCTC 894020 1.6558986 37.715374 40
AGTCA 831270 1.5845257 38.791805 28
ATCAC 851125 1.5670205 37.34142 34
ACATC 846675 1.5588275 37.4765 32
GATCT 811090 1.5553613 38.7652 39
ACGAT 797600 1.5203457 38.438923 37
GTATG 693495 1.3768336 40.02827 45
TCGTA 715980 1.3729768 38.694447 43
TATGC 715775 1.3725837 38.682713 46
CGTAT 678505 1.3011141 38.62583 44
Produced by FastQC (version 0.10.1)