##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW182_ATCACG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10329059	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80141356535963	34.0	31.0	34.0	31.0	34.0
2	32.98110602330764	34.0	33.0	34.0	31.0	34.0
3	33.04479836933839	34.0	33.0	34.0	31.0	34.0
4	36.384514988248206	37.0	37.0	37.0	35.0	37.0
5	36.32757466096379	37.0	37.0	37.0	35.0	37.0
6	36.32321046863998	37.0	37.0	37.0	35.0	37.0
7	36.30170115206042	37.0	37.0	37.0	35.0	37.0
8	36.3007161639797	37.0	37.0	37.0	35.0	37.0
9	38.08685447532055	39.0	38.0	39.0	37.0	39.0
10	38.051039983409915	39.0	38.0	39.0	37.0	39.0
11	38.04879912100415	39.0	38.0	39.0	35.0	39.0
12	38.03672841833898	39.0	38.0	39.0	35.0	39.0
13	38.02922521790223	39.0	38.0	39.0	35.0	39.0
14	39.578966680314245	41.0	40.0	41.0	37.0	41.0
15	39.527387151143195	41.0	39.0	41.0	37.0	41.0
16	39.50910658947732	41.0	39.0	41.0	37.0	41.0
17	39.485097335584975	41.0	39.0	41.0	37.0	41.0
18	39.3987207353545	41.0	39.0	41.0	37.0	41.0
19	39.47626932908409	41.0	39.0	41.0	37.0	41.0
20	39.46721526133213	41.0	39.0	41.0	37.0	41.0
21	39.436628641582935	41.0	39.0	41.0	37.0	41.0
22	39.33260658110289	40.0	39.0	41.0	36.0	41.0
23	39.279737195808444	40.0	39.0	41.0	36.0	41.0
24	39.241931331789274	40.0	39.0	41.0	36.0	41.0
25	39.22485794688558	40.0	39.0	41.0	36.0	41.0
26	39.12277836732272	40.0	39.0	41.0	36.0	41.0
27	39.04226977501048	40.0	39.0	41.0	36.0	41.0
28	38.86008909427277	40.0	38.0	41.0	35.0	41.0
29	38.63009844362396	40.0	38.0	41.0	35.0	41.0
30	38.57036754267741	40.0	38.0	41.0	35.0	41.0
31	38.83779461420445	40.0	38.0	41.0	35.0	41.0
32	38.870772352060335	40.0	38.0	41.0	35.0	41.0
33	38.89463909539097	40.0	38.0	41.0	35.0	41.0
34	39.02862555049787	40.0	39.0	41.0	36.0	41.0
35	39.0795447097359	40.0	39.0	41.0	36.0	41.0
36	39.06002608756519	40.0	39.0	41.0	36.0	41.0
37	38.995527569355545	40.0	39.0	41.0	35.0	41.0
38	38.91832586104891	40.0	39.0	41.0	35.0	41.0
39	38.878381564090205	40.0	39.0	41.0	35.0	41.0
40	38.74410728024692	40.0	38.0	41.0	35.0	41.0
41	38.78080607342837	40.0	38.0	41.0	35.0	41.0
42	38.76947890412863	40.0	38.0	41.0	35.0	41.0
43	38.73359577092163	40.0	38.0	41.0	35.0	41.0
44	38.6850228079828	40.0	38.0	41.0	35.0	41.0
45	38.58579189062624	40.0	38.0	41.0	35.0	41.0
46	38.482873802928225	40.0	38.0	41.0	34.0	41.0
47	38.40728656889267	40.0	38.0	41.0	34.0	41.0
48	38.369695245229984	40.0	38.0	41.0	34.0	41.0
49	38.31430113817725	40.0	38.0	41.0	34.0	41.0
50	38.21510381536208	40.0	38.0	41.0	34.0	41.0
51	37.260473388718175	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	10.0
12	9.0
13	7.0
14	13.0
15	29.0
16	61.0
17	138.0
18	317.0
19	750.0
20	1353.0
21	2281.0
22	4010.0
23	6628.0
24	9985.0
25	15187.0
26	22030.0
27	31198.0
28	43072.0
29	57826.0
30	76743.0
31	100936.0
32	129771.0
33	170814.0
34	228838.0
35	325702.0
36	500922.0
37	868986.0
38	1949862.0
39	5777890.0
40	3680.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.21186645224103	26.69109109512432	31.433282490540105	20.663759962094545
2	18.55212560989341	33.08558891957147	29.793711121216365	18.56857434931875
3	18.52772842134022	29.67607213783947	33.67643654663992	18.11976289418039
4	18.196391365370264	28.66124590826715	30.196622945033035	22.94573978132955
5	22.63360098920918	29.065871344136962	30.563345605829145	17.737182060824708
6	22.7211791509759	29.631392365945437	28.91238204758052	18.73504643549814
7	18.875185048318535	33.67941842524086	29.671231425824946	17.77416510061565
8	18.213730795806278	34.176307832107454	29.446893468223966	18.1630679038623
9	22.13805730028263	29.384516053204845	29.938341914786236	18.539084731726287
10	18.169689997898164	33.73769091647168	29.482230665929976	18.61038841970019
11	22.289281143616275	29.493925826157056	29.561415033063515	18.65537799716315
12	18.129628265265985	29.586596416963058	29.474127314017668	22.809648003753296
13	17.97897562595005	33.84874653150882	29.612649129025208	18.559628713515917
14	17.975712986052265	29.808330071500222	29.69163018625414	22.52432675619338
15	17.93974649578437	33.924397178871764	29.722378388970377	18.413477936373486
16	17.97582916314061	29.679150830680705	29.777717408720388	22.567302597458298
17	22.194354781011512	29.623511686785797	29.78962556027611	18.39250797192658
18	18.178993846390075	29.445722015916452	33.90361116148141	18.471672976212066
19	18.13015204710974	29.404813908619925	29.72294059466522	22.742093449605107
20	18.187474773839515	29.417800789016695	33.87590292591029	18.5188215112335
21	22.415300767615918	29.372121997065946	29.646045761826144	18.56653147349199
22	18.24960139133762	33.63358673830021	29.59195775256454	18.524854117797634
23	18.250103905883392	33.70722347505228	29.548054667903433	18.494617951160894
24	18.211618863061105	29.519317009470875	29.550539997955255	22.718524129512762
25	18.18829769488198	29.464397482868478	33.72565690640357	18.621647915845966
26	18.28450205487994	29.445943590151575	29.53813725576649	22.73141709920199
27	18.250578235172846	29.499041839869054	29.443745663175626	22.80663426178247
28	18.331246638767386	33.67920182672693	29.41497782701231	18.57457370749337
29	22.515366504938612	29.528599970126912	29.42342018757646	18.53261333735801
30	18.441760595768905	29.39773755796485	33.54169591246503	18.618805933801212
31	18.36160265844258	29.446423805116755	29.414919106626307	22.77705442981435
32	18.416754061903454	33.56755482974722	29.37254468983181	18.64314641851751
33	18.333238762957514	29.538036947516154	29.432734609252314	22.69598968027402
34	18.341563254459196	33.64936178815894	29.425387610597532	18.583687346784327
35	18.36394678631367	29.6309149718977	33.53360249777579	18.47153574401284
36	18.31638519518807	29.512906025840042	29.445250991365125	22.72545778760677
37	18.36838287210252	33.64670491074499	29.387849385612082	18.59706283154042
38	18.416858697546523	29.553465269843727	29.414012122151007	22.615663910458743
39	22.474288556127114	29.547816333162523	29.40461347720045	18.573281633509914
40	18.425671380699363	33.61827801004267	29.431515469384482	18.52453513987348
41	18.337328081709373	29.53328738581949	33.49372359413873	18.635660938332407
42	18.376710687947007	29.471415410721	29.490626668518072	22.661247232813913
43	18.332670336582858	29.553925844555863	33.48861143303358	18.6247923858277
44	18.47846410623046	29.475223901234703	29.438370599935144	22.607941392599688
45	22.507943335746848	29.518220987178623	29.43198721822838	18.541848458846154
46	18.35409189348571	29.58489173835935	33.53209261295441	18.528923755200534
47	18.391695593393834	33.73241582411956	29.416862160703793	18.459026421782813
48	18.454483694007394	29.521649885892675	33.54003876695328	18.483827653146655
49	22.561287164806885	29.50669623729705	29.388984988596327	18.54303160929974
50	18.605141610158388	29.412620213733316	29.29859502884665	22.683643147261648
51	18.475823833213592	29.466515103689606	29.35105161339047	22.70660944970633
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.87562641433558
2	37.12069995921216
3	36.64749131552061
4	41.14213114669981
5	40.370783050033886
6	41.45622558647404
7	36.64935014893418
8	36.376798699668576
9	40.677142032008916
10	36.78007841759835
11	40.94465914077943
12	40.93927626901928
13	36.53860433946597
14	40.50003974224564
15	36.35322443215785
16	40.54313176059891
17	40.586862752938096
18	36.65066682260213
19	40.87224549671485
20	36.706296285073016
21	40.98183224110791
22	36.77445550913525
23	36.74472185704429
24	40.93014299257386
25	36.809945610727944
26	41.015919154081935
27	41.05721249695532
28	36.90582034626076
29	41.04797984229662
30	37.060566529570124
31	41.13865708825693
32	37.059900480420964
33	41.02922844323154
34	36.92525060124352
35	36.83548253032651
36	41.04184298279484
37	36.965445703642935
38	41.032522608005266
39	41.04757018963703
40	36.95020652057284
41	36.97298902004178
42	41.03795792076092
43	36.957462722410554
44	41.08640549883015
45	41.049791794593006
46	36.883015648686246
47	36.85072201517664
48	36.93831134715405
49	41.10431877410662
50	41.28878475742004
51	41.182433282919924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	135.5
2	121.0
3	230.5
4	340.0
5	660.5
6	981.0
7	1838.0
8	2695.0
9	5018.5
10	7342.0
11	12317.5
12	17293.0
13	26321.0
14	35349.0
15	49789.0
16	64229.0
17	86509.5
18	108790.0
19	139728.5
20	170667.0
21	208495.0
22	246323.0
23	288568.5
24	330814.0
25	372896.5
26	453129.5
27	491280.0
28	522560.0
29	553840.0
30	579266.5
31	604693.0
32	618606.5
33	632520.0
34	649854.5
35	667189.0
36	673995.0
37	680801.0
38	679295.0
39	677789.0
40	668877.0
41	659965.0
42	642216.0
43	624467.0
44	597745.5
45	571024.0
46	531951.0
47	492878.0
48	454270.5
49	415663.0
50	388610.5
51	361558.0
52	516984.5
53	672411.0
54	442853.5
55	213296.0
56	186551.5
57	159807.0
58	139035.0
59	118263.0
60	103343.0
61	88423.0
62	78916.5
63	69410.0
64	58608.0
65	47806.0
66	41969.0
67	36132.0
68	31963.5
69	27795.0
70	24407.5
71	21020.0
72	18060.5
73	15101.0
74	12852.0
75	8792.5
76	6982.0
77	5568.0
78	4154.0
79	3201.5
80	2249.0
81	1655.0
82	1061.0
83	747.0
84	433.0
85	319.0
86	205.0
87	152.0
88	99.0
89	72.5
90	46.0
91	28.0
92	10.0
93	7.5
94	5.0
95	5.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04282093848045596
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015006207245016221
20	0.0
21	0.013244188071730443
22	5.808854417425633E-5
23	0.0
24	0.0012682665478045967
25	0.0
26	0.09176053694726694
27	0.11511213170531796
28	0.12491941424673826
29	0.7003058071408054
30	0.13735036270002912
31	0.03499834786498945
32	0.12487100712659303
33	0.01600339392000762
34	0.012556806965668412
35	0.017339430436015515
36	0.030457759995368407
37	0.04016822829649826
38	0.0083163432409477
39	0.01695217347485381
40	0.026401243327199505
41	0.012905338230713949
42	0.01724261619572509
43	0.02140562852821346
44	0.015741995471223468
45	0.023990568743967867
46	0.01930475951391119
47	0.009216715675648673
48	0.03150335379050502
49	0.012043691492129147
50	0.014677038828028767
51	0.010833513488498807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.0329059E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.66545785946192
#Duplication Level	Relative count
1	100.0
2	23.935402016493676
3	4.7139251440834675
4	1.343654253667886
5	0.7268285440483743
6	0.5763338687254518
7	0.5027736911012431
8	0.4109921850747075
9	0.3468801036591127
10++	2.4160131733455708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC	394187	3.816291493736264	TruSeq Adapter, Index 1 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4909240	3.9012132	4.262089	1
AAAAA	4907455	3.784567	3.9694939	3
TTTTC	2504095	3.0471182	3.4120917	2
GAAAA	2442435	3.0041175	3.2499301	2
GGAAG	930930	2.9125872	65.70619	5
CGGAA	936170	2.8290513	63.458767	4
GAGCA	929085	2.8076406	63.270065	9
CTCCA	954380	2.706829	58.329662	24
TCCAG	910625	2.67396	60.284466	25
GAAGA	1270905	2.4931076	41.78845	6
TCGGA	813220	2.4722893	63.445213	3
TCTCG	830710	2.4539738	60.146343	41
CTGAA	1284240	2.4479547	40.214348	19
AGAGC	783640	2.3681145	62.905445	8
GCACA	803980	2.3466887	60.6599	11
ATCGG	765710	2.3278527	63.109077	2
CACAC	825290	2.3267033	58.503616	12
AGCAC	779735	2.2759216	60.660587	10
CCAGT	754170	2.2145455	59.646564	26
GTCTG	714485	2.1851912	62.732986	17
CACGA	739595	2.1587594	58.58095	36
GTCAC	732740	2.1516182	59.53131	29
GATCG	705510	2.1448371	62.80938	1
CTCGT	725615	2.143516	59.649963	42
CGTCT	721725	2.1320248	60.58786	16
CACGT	721200	2.1177323	60.40724	14
ATGCC	712210	2.091334	59.24627	47
TCTGA	1074380	2.0602512	40.047943	18
TCACG	697680	2.0486681	58.95838	35
ACACG	696770	2.0337598	60.130936	13
ACTCC	714675	2.0269735	57.91584	23
CGATC	687365	2.0183792	58.821728	38
ACGTC	686450	2.0156922	60.235157	15
CAGTC	678170	1.9913789	59.32793	27
AAGAG	976480	1.9155402	41.235855	7
CATCA	1028405	1.8934137	37.810307	33
TGAAC	987935	1.8831528	39.6218	20
TCACA	989755	1.8222545	37.78985	30
GAACT	902190	1.71971	39.33896	21
AACTC	924745	1.7025635	38.034824	22
CACAT	911315	1.6778374	37.647266	31
ATCTC	894020	1.6558986	37.715374	40
AGTCA	831270	1.5845257	38.791805	28
ATCAC	851125	1.5670205	37.34142	34
ACATC	846675	1.5588275	37.4765	32
GATCT	811090	1.5553613	38.7652	39
ACGAT	797600	1.5203457	38.438923	37
GTATG	693495	1.3768336	40.02827	45
TCGTA	715980	1.3729768	38.694447	43
TATGC	715775	1.3725837	38.682713	46
CGTAT	678505	1.3011141	38.62583	44
>>END_MODULE