##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW180_GAGTGG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9174985	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.828788493932144	34.0	31.0	34.0	31.0	34.0
2	33.016279590647834	34.0	33.0	34.0	31.0	34.0
3	33.07713560294649	34.0	33.0	34.0	31.0	34.0
4	36.404466601307796	37.0	37.0	37.0	35.0	37.0
5	36.35411338547147	37.0	37.0	37.0	35.0	37.0
6	36.35440199629754	37.0	37.0	37.0	35.0	37.0
7	36.345610592278895	37.0	37.0	37.0	35.0	37.0
8	36.32887149134304	37.0	37.0	37.0	35.0	37.0
9	38.133283705640935	39.0	39.0	39.0	37.0	39.0
10	38.10897794383315	39.0	39.0	39.0	37.0	39.0
11	38.09766599073459	39.0	38.0	39.0	37.0	39.0
12	38.082290815734304	39.0	38.0	39.0	37.0	39.0
13	38.06528479338113	39.0	38.0	39.0	37.0	39.0
14	39.60646387977746	41.0	40.0	41.0	37.0	41.0
15	39.57549510980127	41.0	40.0	41.0	37.0	41.0
16	39.53526027562988	41.0	39.0	41.0	37.0	41.0
17	39.52251322481726	41.0	39.0	41.0	37.0	41.0
18	39.484938449490656	41.0	39.0	41.0	37.0	41.0
19	39.53089470990961	41.0	40.0	41.0	37.0	41.0
20	39.50840224806907	41.0	39.0	41.0	37.0	41.0
21	39.48200089700419	41.0	39.0	41.0	37.0	41.0
22	39.42620647336208	41.0	39.0	41.0	37.0	41.0
23	39.368367032752644	41.0	39.0	41.0	37.0	41.0
24	39.29356996223972	40.0	39.0	41.0	36.0	41.0
25	39.2870097335309	40.0	39.0	41.0	36.0	41.0
26	39.1595011871954	40.0	39.0	41.0	36.0	41.0
27	39.073197721849134	40.0	39.0	41.0	36.0	41.0
28	39.002941149222586	40.0	39.0	41.0	36.0	41.0
29	38.71261271816793	40.0	38.0	41.0	35.0	41.0
30	38.74826465656347	40.0	38.0	41.0	35.0	41.0
31	38.963868169811725	40.0	39.0	41.0	36.0	41.0
32	38.960823260201515	40.0	39.0	41.0	36.0	41.0
33	38.96334184742536	40.0	39.0	41.0	36.0	41.0
34	39.10684867604688	40.0	39.0	41.0	36.0	41.0
35	39.16179917460356	40.0	39.0	41.0	36.0	41.0
36	39.1574741539087	40.0	39.0	41.0	36.0	41.0
37	39.09125617099102	40.0	39.0	41.0	36.0	41.0
38	39.058903311558545	40.0	39.0	41.0	36.0	41.0
39	39.03791810013858	40.0	39.0	41.0	35.0	41.0
40	38.983027438191996	40.0	39.0	41.0	35.0	41.0
41	38.96762076450261	40.0	39.0	41.0	35.0	41.0
42	38.92061698193512	40.0	39.0	41.0	35.0	41.0
43	38.877316093704785	40.0	38.0	41.0	35.0	41.0
44	38.81503969761258	40.0	38.0	41.0	35.0	41.0
45	38.749812996969474	40.0	38.0	41.0	35.0	41.0
46	38.72535628123643	40.0	38.0	41.0	35.0	41.0
47	38.65132019289405	40.0	38.0	41.0	35.0	41.0
48	38.575125299932374	40.0	38.0	41.0	35.0	41.0
49	38.52939999356947	40.0	38.0	41.0	34.0	41.0
50	38.420948263130676	40.0	38.0	41.0	34.0	41.0
51	37.46308271893633	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	0.0
13	4.0
14	5.0
15	12.0
16	41.0
17	79.0
18	260.0
19	508.0
20	986.0
21	1686.0
22	2928.0
23	4801.0
24	7524.0
25	11576.0
26	16935.0
27	24607.0
28	34342.0
29	46941.0
30	62022.0
31	81625.0
32	106149.0
33	139320.0
34	189175.0
35	269025.0
36	413319.0
37	727719.0
38	1720354.0
39	5309560.0
40	3473.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.26205290257578	30.271753234716197	31.938081872722396	19.52811198998563
2	18.792957154698346	31.233914823838948	31.348759698244738	18.624368323217965
3	18.63132201306051	30.916955177583393	31.642940015705744	18.808782793650344
4	18.7913876698436	30.600703979352556	31.59912523017749	19.008783120626354
5	19.085731475310315	31.326023966251714	31.452770767472643	18.135473790965325
6	19.089829574653255	31.479811683615832	30.711036584800954	18.71932215692996
7	18.86235236351885	31.701000056130884	31.227288110007812	18.20935947034246
8	18.419681340078487	31.756575078869336	31.313653373820227	18.510090207231947
9	18.555463578414567	31.135527742007206	31.574536634119838	18.734472045458386
10	18.40804099407247	31.50313597242938	31.32676511187757	18.76205792162058
11	18.688869791067777	31.136083601226595	31.379048576101216	18.795998031604412
12	18.44683124822547	31.105129872146932	31.340487205156194	19.107551674471402
13	18.39741427370181	31.51413326561297	31.38362624026088	18.70482622042434
14	18.39403552158396	31.298394493288	31.413686234909378	18.893883750218667
15	18.33484196431929	31.549021606029875	31.41923392790288	18.696902501747957
16	18.39803552812348	31.283669673574398	31.416825204618863	18.90146959368326
17	18.684433816513053	31.23650883352943	31.42841105462298	18.650646295334543
18	18.485708695981522	31.161969202129487	31.610362305769435	18.74195979611956
19	18.48442776989028	31.133473673380013	31.34826718844041	19.0338313682893
20	18.52018286678398	31.08441049222424	31.615811905959518	18.779594735032266
21	18.80000130807869	31.079709955350914	31.302334048416352	18.81795468815404
22	18.56069519459705	31.365326482822585	31.293097481903242	18.780880840677124
23	18.566482669998916	31.394100371826223	31.294656067557604	18.74476089061726
24	18.539433979003917	31.084069566665583	31.290579664809925	19.085916789520574
25	18.54686410931462	31.15161496176833	31.55090716769564	18.75061376122141
26	18.557556349443846	31.170587155264702	31.26814981251671	19.003706682774745
27	18.5779892346013	31.156203907724716	31.21972170236419	19.046085155309793
28	18.573496584504525	31.453049282552243	31.218136092721195	18.755318040222036
29	18.884586993932704	31.188844552584627	31.185595077660256	18.740973375822414
30	18.616172324516114	31.14536183754199	31.422863447706202	18.815602390235696
31	18.60172938476734	31.14548762546882	31.211582235399955	19.041200754363885
32	18.62190528136068	31.423819934753716	31.17522317506734	18.779051608818268
33	18.624232717036058	31.169568758331796	31.175782348194097	19.030416176438045
34	18.848675873698806	31.209735131137258	31.207435099034914	18.734153896129023
35	18.60557268602891	31.47997914837553	31.204203115403622	18.710245050191933
36	18.821774996557515	31.269855616233176	31.184804701796843	18.723564685412466
37	18.601929298315053	31.271751491896925	31.42158995377927	18.704729256008747
38	18.845077668812902	31.31234188011829	31.188865308293572	18.653715142775237
39	18.840952876278852	31.24478994235811	31.21526968898974	18.6989874923733
40	18.61945154056885	31.48216791664086	31.196203067095663	18.702177475694622
41	18.60323736368503	31.231763762905235	31.45975249460441	18.70524637880532
42	18.61538911003814	31.185565661705745	31.219708257406392	18.979336970849722
43	18.57182009774017	31.22315186966117	31.4798102931987	18.725217739399962
44	18.60214468859218	31.25341133812971	31.24433078798136	18.900113185296746
45	18.822627887386254	31.278362017547945	31.228736492934082	18.67027360213172
46	18.592918205536673	31.284544517916302	31.487430622076857	18.63510665447017
47	18.62618911209766	31.57547706187612	31.23075554671906	18.56757827930716
48	18.625798779563063	31.248966278857925	31.501823873869505	18.62341106770951
49	18.87092218805617	31.30398308317045	31.207818158253108	18.61727657052027
50	18.64783582243304	31.266150405988707	31.216169651576912	18.86984412000134
51	18.65352591007904	31.24015617783536	31.16848546763258	18.937832444453022
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.79016489256141
2	37.417325477916314
3	37.440104806710856
4	37.800170790469956
5	37.22120526627564
6	37.80915173158321
7	37.07171183386131
8	36.92977154731043
9	37.28993562387296
10	37.17009891569305
11	37.48486782267219
12	37.554382922696874
13	37.10224049412615
14	37.28791927180262
15	37.031744466067245
16	37.29950512180674
17	37.335080111847596
18	37.22766849210108
19	37.51825913817958
20	37.299777601816245
21	37.61795599623273
22	37.34157603527417
23	37.31124356061618
24	37.62535076852449
25	37.29747787053603
26	37.56126303221859
27	37.624074389911094
28	37.32881462472656
29	37.62556036975512
30	37.43177471475181
31	37.642930139131224
32	37.400956890178946
33	37.65464889347411
34	37.58282976982783
35	37.31581773622084
36	37.54533968196998
37	37.3066585543238
38	37.49879281158814
39	37.539940368652154
40	37.32162901626347
41	37.30848374249035
42	37.59472608088787
43	37.29703783714013
44	37.50225787388893
45	37.49290148951797
46	37.22802486000684
47	37.19376739140482
48	37.249209847272574
49	37.48819875857644
50	37.51767994243438
51	37.591358354532055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	135.0
1	137.0
2	139.0
3	229.5
4	320.0
5	643.5
6	967.0
7	1863.5
8	2760.0
9	5119.0
10	7478.0
11	12201.5
12	16925.0
13	26016.5
14	35108.0
15	49671.5
16	64235.0
17	86181.5
18	108128.0
19	138210.5
20	168293.0
21	204676.5
22	241060.0
23	281978.5
24	322897.0
25	363269.5
26	438870.5
27	474099.0
28	505080.0
29	536061.0
30	560286.0
31	584511.0
32	599183.5
33	613856.0
34	629883.5
35	645911.0
36	653416.0
37	660921.0
38	659089.0
39	657257.0
40	648524.5
41	639792.0
42	622785.0
43	605778.0
44	576466.5
45	547155.0
46	503913.5
47	460672.0
48	417325.5
49	373979.0
50	333996.5
51	294014.0
52	256568.5
53	219123.0
54	197920.0
55	176717.0
56	142799.0
57	108881.0
58	91958.5
59	75036.0
60	62369.0
61	49702.0
62	40583.0
63	31464.0
64	25320.5
65	19177.0
66	15484.5
67	11792.0
68	9398.5
69	7005.0
70	5613.5
71	4222.0
72	3333.5
73	2445.0
74	1953.0
75	1145.5
76	830.0
77	652.0
78	474.0
79	378.5
80	283.0
81	214.5
82	146.0
83	102.5
84	59.0
85	47.0
86	35.0
87	29.5
88	24.0
89	15.5
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	1.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04539516958338352
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.001384198448280842
20	0.0
21	0.013351520465701034
22	0.0
23	0.0
24	0.0014277952498014982
25	0.0
26	0.09338434885724609
27	0.1157931048388635
28	0.12643072440990366
29	0.7173962682227818
30	0.14033810409499306
31	0.035792974048458936
32	0.12589666359127563
33	0.016828365386973385
34	0.013079040456196931
35	0.01742782140788241
36	0.030866535476624758
37	0.04114448143511951
38	0.008446880294627184
39	0.017678503016626185
40	0.027564077761435032
41	0.014016371688891045
42	0.01845234624361784
43	0.02281202639568348
44	0.016697574982411416
45	0.024915572069055154
46	0.020250714306344914
47	0.00995096994708983
48	0.032959181949616265
49	0.012664870841750695
50	0.015847437352758613
51	0.011814733212097894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9174985.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.650474180218207
#Duplication Level	Relative count
1	100.0
2	6.098365746671325
3	1.0464323146284251
4	0.630521219784059
5	0.4591658487081801
6	0.3992746510505914
7	0.3388289052665436
8	0.2672921969533126
9	0.23124656873346752
10++	1.4384978400350474
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC	20623	0.22477420944012444	TruSeq Adapter, Index 7 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4678900	3.5945184	3.8157516	15
AAAAA	4612590	3.5940979	3.7343867	6
TTTTC	2392380	3.0661678	3.2726314	2
GAAAA	2333720	3.0499566	3.175481	6
CTCCA	512405	1.8329314	5.839377	24
GAGCA	486130	1.772704	5.8831844	9
CGGAA	483690	1.7638067	5.9516644	4
GGAAG	472520	1.7372595	5.9091787	5
TCCAG	476605	1.7189059	5.7029533	25
GTGGA	408825	1.49883	5.0890155	36
TCTCG	392205	1.4105128	5.1047626	41
TCGGA	386775	1.4064121	5.584874	3
CACAC	358935	1.2875922	5.3799434	12
GCACA	352565	1.2751533	5.3732934	11
AGAGC	342095	1.2474715	5.347339	8
AGCAC	325365	1.1767765	5.272263	10
ATCGG	315585	1.1475471	5.2340593	2
CCAGT	317835	1.1462921	5.054038	26
CACGT	285385	1.0292591	5.0617933	14
GTCTG	280445	1.0168861	5.054088	17
CGTCT	282715	1.0167468	5.055146	16
ACACG	264640	0.95714694	5.0319967	13
GATCG	253775	0.9227904	5.0075912	1
>>END_MODULE