##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW179_CGTACG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8391404	
Filtered Sequences	0	
Sequence length	51	
%GC	36	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.821916332475475	34.0	31.0	34.0	31.0	34.0
2	33.011245674740486	34.0	33.0	34.0	31.0	34.0
3	33.071570859894244	34.0	33.0	34.0	31.0	34.0
4	36.39940801324784	37.0	37.0	37.0	35.0	37.0
5	36.34595652884786	37.0	37.0	37.0	35.0	37.0
6	36.344502660103124	37.0	37.0	37.0	35.0	37.0
7	36.33550631098205	37.0	37.0	37.0	35.0	37.0
8	36.31944034633537	37.0	37.0	37.0	35.0	37.0
9	38.12435332633252	39.0	39.0	39.0	37.0	39.0
10	38.099936315782195	39.0	38.0	39.0	37.0	39.0
11	38.089288633940164	39.0	38.0	39.0	37.0	39.0
12	38.07426802475486	39.0	38.0	39.0	37.0	39.0
13	38.059195338467795	39.0	38.0	39.0	37.0	39.0
14	39.59810646704651	41.0	40.0	41.0	37.0	41.0
15	39.568327302558664	41.0	40.0	41.0	37.0	41.0
16	39.527844684870374	41.0	39.0	41.0	37.0	41.0
17	39.51364884827378	41.0	39.0	41.0	37.0	41.0
18	39.47362860851414	41.0	39.0	41.0	37.0	41.0
19	39.52447707201322	41.0	40.0	41.0	37.0	41.0
20	39.50297351909168	41.0	39.0	41.0	37.0	41.0
21	39.47713481558033	41.0	39.0	41.0	37.0	41.0
22	39.41956828678491	41.0	39.0	41.0	37.0	41.0
23	39.36373519854365	41.0	39.0	41.0	36.0	41.0
24	39.291158189976315	40.0	39.0	41.0	36.0	41.0
25	39.284616138133735	40.0	39.0	41.0	36.0	41.0
26	39.160439421102836	40.0	39.0	41.0	36.0	41.0
27	39.073792657343155	40.0	39.0	41.0	36.0	41.0
28	38.997426294813124	40.0	39.0	41.0	36.0	41.0
29	38.714073949961175	40.0	38.0	41.0	35.0	41.0
30	38.743296473391105	40.0	38.0	41.0	35.0	41.0
31	38.95891164339126	40.0	39.0	41.0	36.0	41.0
32	38.953447480302465	40.0	39.0	41.0	36.0	41.0
33	38.96011561354929	40.0	39.0	41.0	36.0	41.0
34	39.092370001492	40.0	39.0	41.0	36.0	41.0
35	39.15146178160413	40.0	39.0	41.0	36.0	41.0
36	39.14335229241733	40.0	39.0	41.0	36.0	41.0
37	39.08638375652036	40.0	39.0	41.0	36.0	41.0
38	39.04935932056185	40.0	39.0	41.0	36.0	41.0
39	39.02623422731166	40.0	39.0	41.0	35.0	41.0
40	38.96986010922606	40.0	39.0	41.0	35.0	41.0
41	38.9570131529837	40.0	39.0	41.0	35.0	41.0
42	38.911844787832884	40.0	39.0	41.0	35.0	41.0
43	38.868536897997046	40.0	38.0	41.0	35.0	41.0
44	38.806822076496374	40.0	38.0	41.0	35.0	41.0
45	38.7393479088839	40.0	38.0	41.0	35.0	41.0
46	38.710442018999444	40.0	38.0	41.0	35.0	41.0
47	38.63775680446324	40.0	38.0	41.0	35.0	41.0
48	38.56638900951498	40.0	38.0	41.0	35.0	41.0
49	38.52189562080434	40.0	38.0	41.0	34.0	41.0
50	38.4175087982893	40.0	38.0	41.0	34.0	41.0
51	37.46682688618019	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	4.0
13	1.0
14	3.0
15	18.0
16	35.0
17	85.0
18	230.0
19	428.0
20	884.0
21	1575.0
22	2716.0
23	4573.0
24	6849.0
25	10644.0
26	15861.0
27	22680.0
28	31421.0
29	42881.0
30	56872.0
31	75325.0
32	98216.0
33	129043.0
34	174374.0
35	247484.0
36	380868.0
37	666895.0
38	1573692.0
39	4844602.0
40	3138.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.847313791927665	30.120832270263698	32.60090418543432	19.43094975237432
2	18.558086346456445	31.46362634905911	31.526571715531755	18.451715588952695
3	18.532881982562156	30.914338053560524	31.859185900237907	18.69359406363941
4	18.75546690398889	30.50301236837125	31.7242025291596	19.017318198480254
5	19.249210263264647	31.267163397209814	31.675116583589585	17.808509755935955
6	19.229916710004666	31.584106783560888	30.770023705210715	18.415952801223728
7	18.822237613634144	32.00655098956027	31.388728274791678	17.782483122013907
8	18.229273671009047	32.143035897211	31.52605928638402	18.10163114539593
9	18.456291700411516	31.342990994117315	31.871519950654264	18.329197354816905
10	18.128837558053455	31.936634203287078	31.62038200043759	18.314146238221877
11	18.528818300251064	31.392541701007364	31.665344678911893	18.413295319829672
12	18.111557970513637	31.389765050044065	31.629546140312158	18.86913083913014
13	18.085138077013095	31.93433423060074	31.669742036016856	18.310785656369305
14	18.089678437601144	31.59739419053117	31.675986521445044	18.636940850422647
15	18.04391732301293	32.016132222927176	31.684900405224205	18.255050048835688
16	18.10676735383018	31.577135363760345	31.72613307618129	18.58996420622818
17	18.5410927658828	31.54580568400711	31.704849391115005	18.208252158995087
18	18.14903680003966	31.431891492770458	32.11349376099637	18.30557794619351
19	18.135516869893507	31.413753324880112	31.688216815551385	18.762512989674995
20	18.182120439216185	31.36964104810602	32.10335625639717	18.344882256280624
21	18.618681075200357	31.343948002273603	31.652879372669084	18.384491549856957
22	18.215649355292324	31.802964940296018	31.635650785327833	18.345734919083824
23	18.22188515771616	31.811077145135663	31.626507316296532	18.340530380851643
24	18.19321524695474	31.370077276326775	31.64306190388515	18.79364557283334
25	18.227605297039684	31.412740942993565	32.04655621395418	18.313097546012564
26	18.253612027204607	31.437390554344148	31.56894302498161	18.740054393469634
27	18.25779759422063	31.4426849742347	31.544345521304688	18.755171910239977
28	18.26054128366915	31.88614293236466	31.52796068311759	18.325355100848604
29	18.69812590737654	31.454471782029913	31.529102302143908	18.318300008449643
30	18.304623385550233	31.391574251860728	31.900059548031635	18.403742814557404
31	18.284896913736176	31.437695422466582	31.494522836287576	18.782884827509665
32	18.317681427518718	31.866495137624135	31.468670893325545	18.3471525415316
33	18.29170078917907	31.469845869738794	31.492467739699748	18.74598560138239
34	18.28184040633066	31.502544333958493	31.515058624085977	18.70055663562487
35	18.689530129123966	31.519363350457848	31.509255985931695	18.281850534486495
36	18.257728042196728	31.527279450445032	31.910488351448347	18.30450415590989
37	18.257769096668568	31.983269578125988	31.51604665338511	18.242914671820333
38	18.27674317163921	31.595282418475605	31.47397011467403	18.65400429521115
39	18.63105562108286	31.57427077959909	31.511112679104887	18.283560920213166
40	18.277615261187886	31.975953928543372	31.51371836434552	18.23271244592322
41	18.26261153184081	31.554671891419595	31.937243849070303	18.245472727669288
42	18.274655831880118	31.53259714853839	31.52487368017189	18.667873339409606
43	18.222368169332555	31.55797414550914	31.94093433035605	18.27872335480226
44	18.258305568447746	31.561672610581006	31.57924094771763	18.600780873253626
45	18.65004871028306	31.570080288230418	31.584229096744593	18.195641904741926
46	18.23713289543882	31.618561680050313	31.954932654290115	18.189372770220757
47	18.255623251253507	32.052523206477495	31.562263389988836	18.129590152280162
48	18.278648531341705	31.58258759897677	31.923617347170502	18.215146522511027
49	18.663141215303412	31.567485040966385	31.566567327122673	18.202806416607523
50	18.288244103399016	31.578487559615574	31.506272485270042	18.62699585171536
51	18.284537778069115	31.55680565404716	31.51369711993993	18.644959447943794
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.278263544301986
2	37.00980193540914
3	37.22647604620157
4	37.77278510246915
5	37.0577200192006
6	37.6458695112284
7	36.60472073564805
8	36.33090481640498
9	36.78548905522842
10	36.44298379627533
11	36.94211362008074
12	36.98068880964377
13	36.3959237333824
14	36.72661928802379
15	36.298967371848626
16	36.69673156005837
17	36.74934492487789
18	36.45461474623317
19	36.8980298595685
20	36.527002695496805
21	37.00317262505731
22	36.561384274376145
23	36.5624155385678
24	36.98686081978808
25	36.540702843052244
26	36.993666420674245
27	37.01296950446061
28	36.58589638451775
29	37.01642591582618
30	36.70836620010764
31	37.067781741245845
32	36.66483396905032
33	37.03768639056146
34	36.98239704195553
35	36.97138066361046
36	36.562232198106614
37	36.5006837684889
38	36.93074746685036
39	36.91461654129603
40	36.51032770711111
41	36.5080842595101
42	36.94252917128972
43	36.50109152413481
44	36.859086441701365
45	36.845690615024985
46	36.426505665659576
47	36.385213403533676
48	36.49379505385273
49	36.86594763191094
50	36.91523995511438
51	36.929497226012906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	163.0
2	144.0
3	261.5
4	379.0
5	768.5
6	1158.0
7	2152.0
8	3146.0
9	5681.0
10	8216.0
11	13430.0
12	18644.0
13	27780.5
14	36917.0
15	51695.5
16	66474.0
17	87916.5
18	109359.0
19	138587.5
20	167816.0
21	202933.5
22	238051.0
23	276767.5
24	315484.0
25	353273.0
26	424729.0
27	458396.0
28	485910.5
29	513425.0
30	534364.0
31	555303.0
32	568362.5
33	581422.0
34	594236.0
35	607050.0
36	611154.0
37	615258.0
38	608785.0
39	602312.0
40	588797.5
41	575283.0
42	553879.5
43	532476.0
44	502744.5
45	473013.0
46	432087.0
47	391161.0
48	350697.5
49	310234.0
50	275157.0
51	240080.0
52	208910.5
53	177741.0
54	166925.0
55	156109.0
56	120918.5
57	85728.0
58	71843.5
59	57959.0
60	48348.0
61	38737.0
62	31615.5
63	24494.0
64	19646.0
65	14798.0
66	12006.5
67	9215.0
68	7394.0
69	5573.0
70	4491.0
71	3409.0
72	2796.0
73	2183.0
74	1763.5
75	1049.0
76	754.0
77	589.0
78	424.0
79	341.0
80	258.0
81	194.5
82	131.0
83	90.0
84	49.0
85	39.5
86	30.0
87	20.5
88	11.0
89	8.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04461708672350896
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014777026585777542
20	1.1916956924014147E-5
21	0.014193095696500848
22	4.766782769605659E-5
23	0.0
24	0.0010606091662372589
25	0.0
26	0.09148647830565661
27	0.11425978298744764
28	0.12483012377904817
29	0.7113469927082524
30	0.1384035377155003
31	0.03392757636266827
32	0.12293532762812993
33	0.015408625302750289
34	0.01186928909631809
35	0.017660930161388963
36	0.029875811008503467
37	0.039695383513891125
38	0.007912859397545392
39	0.016064057933571067
40	0.025919381309730764
41	0.012953732176403375
42	0.016576487081303676
43	0.02096192722934088
44	0.015611213570458532
45	0.023893498632648363
46	0.018590452801462067
47	0.008949634649934623
48	0.032092364996370096
49	0.011785870397849991
50	0.01416926178265282
51	0.011368776905509496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8391404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.560106046217161
#Duplication Level	Relative count
1	100.0
2	5.883556568192627
3	0.972205324455554
4	0.6392885978786237
5	0.4556866822249013
6	0.4202935418579187
7	0.3511663145786557
8	0.2842511585723292
9	0.22673730547598245
10++	1.3703781535841084
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC	30821	0.36729252935504	TruSeq Adapter, Index 12 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	4510750	3.6754017	3.7926776	13
TTTTT	4605480	3.6738255	3.870367	13
GAAAA	2242575	3.1376705	3.2991297	7
TTTTC	2285470	3.1288948	3.2851758	1
CTGAA	807600	1.9228415	5.860462	19
CGGAA	458945	1.8843198	9.024942	4
CTCCA	460025	1.8628724	8.688654	24
GAAGA	764715	1.837233	5.9182696	6
GGAAG	438020	1.8070195	8.966481	5
GAGCA	439230	1.8033746	8.779176	9
TCCAG	433450	1.7636629	8.5383835	25
TCACC	366395	1.4837174	7.959529	30
TCTCG	364635	1.4773806	7.893705	41
TCGGA	360525	1.4739639	8.544308	3
TCTGA	618580	1.4665626	5.415957	18
CACCG	205340	1.4339104	12.44355	31
GCACA	330220	1.3493432	8.266367	11
CACAC	326260	1.3268076	8.24224	12
AGAGC	317155	1.3021636	8.256802	8
TGAAC	521730	1.2422043	5.1812825	20
CCAGT	297890	1.2120835	7.949662	26
ATCGG	294385	1.2035584	8.201693	2
AGCAC	293175	1.1979702	8.161814	10
AAGAG	490570	1.1785978	5.2076716	7
GTCAC	276895	1.1266569	7.6235523	29
CTCGT	272420	1.103756	7.4431753	42
AACTC	465680	1.1034684	5.0793433	22
ACTCC	269185	1.0900652	7.8599353	23
GTCTG	266590	1.0853077	7.930257	17
CACGT	262880	1.0696313	7.9018483	14
GAACT	448275	1.0673128	5.056068	21
CGTCT	262130	1.0620642	7.860875	16
ATGCC	248000	1.0090861	7.364435	47
ACACG	244875	1.0006069	7.928729	13
CAGTC	235685	0.95897776	7.63221	27
GATCG	232310	0.949772	7.8701615	1
ACGTC	233270	0.94915134	7.8245707	15
CGATC	226600	0.9220118	7.24226	38
ACCGT	219860	0.89458746	7.261074	32
CCGTA	192705	0.78409666	7.122354	33
GTACG	153860	0.6290385	7.069686	35
CGTAC	154085	0.6269558	6.9661984	34
>>END_MODULE