##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW178_GTTTCG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6143335	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.786766471305896	34.0	31.0	34.0	31.0	34.0
2	32.971886117231115	34.0	31.0	34.0	31.0	34.0
3	33.03279228627447	34.0	33.0	34.0	31.0	34.0
4	36.3730338651563	37.0	37.0	37.0	35.0	37.0
5	36.31526149884387	37.0	37.0	37.0	35.0	37.0
6	36.31309687653368	37.0	37.0	37.0	35.0	37.0
7	36.29467772797674	37.0	37.0	37.0	35.0	37.0
8	36.282963895017936	37.0	37.0	37.0	35.0	37.0
9	38.072090973388235	39.0	38.0	39.0	37.0	39.0
10	38.05046330698228	39.0	38.0	39.0	37.0	39.0
11	38.03542749988402	39.0	38.0	39.0	35.0	39.0
12	38.01895013050729	39.0	38.0	39.0	35.0	39.0
13	38.006611881006	39.0	38.0	39.0	35.0	39.0
14	39.523623569282805	41.0	39.0	41.0	37.0	41.0
15	39.49121006749591	41.0	39.0	41.0	37.0	41.0
16	39.453205140204794	41.0	39.0	41.0	37.0	41.0
17	39.42882180444335	41.0	39.0	41.0	37.0	41.0
18	39.38008182851822	41.0	39.0	41.0	36.0	41.0
19	39.42953135389817	41.0	39.0	41.0	37.0	41.0
20	39.40770819107211	41.0	39.0	41.0	37.0	41.0
21	39.37875274586198	41.0	39.0	41.0	36.0	41.0
22	39.309476530255964	40.0	39.0	41.0	36.0	41.0
23	39.238630646057885	40.0	39.0	41.0	36.0	41.0
24	39.17380396804016	40.0	39.0	41.0	36.0	41.0
25	39.159901584400004	40.0	39.0	41.0	36.0	41.0
26	39.03632245352077	40.0	39.0	41.0	36.0	41.0
27	38.94503685701659	40.0	39.0	41.0	36.0	41.0
28	38.833864667969436	40.0	38.0	41.0	35.0	41.0
29	38.560487585326214	40.0	38.0	41.0	35.0	41.0
30	38.559697297966004	40.0	38.0	41.0	35.0	41.0
31	38.796264081317396	40.0	38.0	41.0	35.0	41.0
32	38.792166958174995	40.0	38.0	41.0	35.0	41.0
33	38.80462729120258	40.0	38.0	41.0	35.0	41.0
34	38.94707614023979	40.0	39.0	41.0	36.0	41.0
35	38.97974243631513	40.0	39.0	41.0	36.0	41.0
36	38.99005833150886	40.0	39.0	41.0	35.0	41.0
37	38.93921754226328	40.0	39.0	41.0	35.0	41.0
38	38.86357019436511	40.0	38.0	41.0	35.0	41.0
39	38.84386330877284	40.0	38.0	41.0	35.0	41.0
40	38.749931429752735	40.0	38.0	41.0	35.0	41.0
41	38.76254379746506	40.0	38.0	41.0	35.0	41.0
42	38.72255102480982	40.0	38.0	41.0	35.0	41.0
43	38.67558012056969	40.0	38.0	41.0	35.0	41.0
44	38.60914649778988	40.0	38.0	41.0	35.0	41.0
45	38.527026118549614	40.0	38.0	41.0	34.0	41.0
46	38.47811473735357	40.0	38.0	41.0	34.0	41.0
47	38.39245767974561	40.0	38.0	41.0	34.0	41.0
48	38.32412557674292	40.0	38.0	41.0	34.0	41.0
49	38.264148056389566	40.0	38.0	41.0	34.0	41.0
50	38.154183192028434	40.0	38.0	41.0	34.0	41.0
51	37.155324917166325	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	2.0
14	3.0
15	19.0
16	24.0
17	80.0
18	212.0
19	414.0
20	798.0
21	1450.0
22	2418.0
23	4133.0
24	6078.0
25	9391.0
26	13603.0
27	19066.0
28	26445.0
29	35277.0
30	46505.0
31	60639.0
32	78963.0
33	102880.0
34	138396.0
35	196424.0
36	303136.0
37	531508.0
38	1235102.0
39	3328638.0
40	1728.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.06366399543489	28.01252929734575	31.801902389516922	21.121904317702437
2	19.03788414598911	30.98655697597478	30.849009536351186	19.12654934168493
3	18.79798187792136	29.624983823932766	32.15647526954008	19.420559028605798
4	19.143136423457292	28.73237419089143	30.703762695669372	21.420726689981905
5	20.981681773824803	29.538434742692694	30.50411543567134	18.975768047811165
6	21.02634806664458	30.015065107144572	29.17348638809376	19.78510043811708
7	19.776326702027482	31.478830309595686	29.835586045690164	18.909256942686667
8	19.05071105515164	31.615694081472036	29.91160338806202	19.421991475314304
9	20.431101348046298	29.612075525752708	30.24816325334692	19.708659872854074
10	19.079880879033944	31.25575929035288	29.86758820738247	19.796771623230704
11	20.598681335138	29.61513575281179	29.909357051178226	19.876825860871985
12	19.13740663662327	29.617561145534143	29.848136232193102	21.39689598564949
13	19.12806317741097	31.253822231735697	29.86281881095529	19.755295779898052
14	19.11533393506947	29.849861679364707	29.832867652504707	21.201936733061114
15	19.081980715686186	31.347891658195426	29.864055924021727	19.70607170209666
16	19.16045600638741	29.853589296367527	29.807327778804183	21.17862691844088
17	20.693727429808074	29.831907262097868	29.82738203272327	19.64698327537079
18	19.287764707605884	29.695775991379275	31.27122320368334	19.7452360973315
19	19.213451266700666	29.651179352664382	29.816987542247634	21.318381838387314
20	19.327368603535376	29.6055481265469	31.239807042917246	19.827276227000482
21	20.818082377060456	29.547606833752514	29.712132383645713	19.922178405541317
22	19.340204957179587	31.099507563647872	29.708340685478174	19.851946793694367
23	19.364188995065383	31.129378423934227	29.715716300673822	19.790716280326563
24	19.362555507806906	29.602704092927556	29.682091651365393	21.352648747900144
25	19.320906315543592	29.629574164521387	31.18669257007798	19.86282694985704
26	19.36983832943128	29.645947482725866	29.67898869136937	21.305225496473486
27	19.39708185191408	29.67854023824497	29.595216261815573	21.329161648025377
28	19.418352504003227	31.166399784864744	29.58924649692169	19.826001214210333
29	20.974808072826587	29.62989981917944	29.60099803769527	19.794294070298708
30	19.51119563372974	29.521909837360248	31.025044308358463	19.941850220551547
31	19.49255017490016	29.566447567892883	29.527204538252832	21.41379771895412
32	19.48882481665339	31.075740455619112	29.51696629427736	19.91846843345014
33	19.511699563327877	29.579562663638082	29.55424668604899	21.354491086985046
34	20.935620400358285	29.666860829896407	29.560244704276595	19.837274065468716
35	19.4666471299315	29.699240102405057	31.082847967569023	19.75126480009443
36	19.473986298079858	29.640876173883306	31.0599001054307	19.825237422606133
37	19.4959584802381	29.683385923490157	31.003196120167082	19.817459476104656
38	19.566298536361746	29.686400905899763	29.515812141055857	21.231488416682634
39	20.997392035835208	29.66120886522219	29.517371151282923	19.82402794765968
40	19.55950203615235	31.144688687928536	29.475566416047855	19.820242859871264
41	19.53040598840219	29.65595665636691	30.97169900399509	19.841938351235807
42	19.576739733520885	29.63131194727725	29.514352882949762	21.2775954362521
43	19.507863487352235	29.615035505564556	31.050839344545217	19.826261662537988
44	19.582072212391573	29.61303266028507	29.554830209204848	21.250064918118515
45	20.97823285445103	29.61390755357773	29.603796628095903	19.804062963875342
46	19.491824828308857	29.731393030500474	31.051308877338073	19.725473263852596
47	19.5349314232225	31.174066989540517	29.591176590289365	19.69982499694762
48	19.6171020956867	29.64221698850551	30.972521467501746	19.768159448306047
49	21.07909652991258	29.647755675421887	29.495962854714374	19.777184939951155
50	19.64263241737981	29.598244338749495	29.495027607219637	21.264095636651057
51	19.648701249545187	29.58372099270101	29.450830140485312	21.316747617268486
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.185568313137324
2	38.16443348767404
3	38.21854090652715
4	40.5638631134392
5	39.95744982163597
6	40.811448504761664
7	38.68558364471415
8	38.472702530465945
9	40.13976122090037
10	38.87665250226465
11	40.475507196009985
12	40.534302622272754
13	38.883358957309014
14	40.31727066813058
15	38.78805241778285
16	40.33908292482829
17	40.340710705178864
18	39.03300080493739
19	40.531833105087976
20	39.15464483053586
21	40.74026078260177
22	39.192151750873954
23	39.15490527539195
24	40.71520425570705
25	39.18373326540063
26	40.675063825904765
27	40.72624349993946
28	39.24435371821357
29	40.769102143125295
30	39.453045854281285
31	40.90634789385428
32	39.40729325010353
33	40.86619065031292
34	40.772894465827
35	39.21791193002593
36	39.299223720685994
37	39.31341795634276
38	40.79778695304438
39	40.82141998349489
40	39.37974489602361
41	39.372344339638
42	40.85433516977299
43	39.33412514989023
44	40.832137130510084
45	40.78229581832637
46	39.21729809216146
47	39.23475642017012
48	39.38526154399275
49	40.85628146986374
50	40.906728054030864
51	40.96544886681367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	21.5
2	26.0
3	55.0
4	84.0
5	176.0
6	268.0
7	576.0
8	884.0
9	1643.5
10	2403.0
11	4247.0
12	6091.0
13	9606.5
14	13122.0
15	19141.0
16	25160.0
17	35068.5
18	44977.0
19	59180.5
20	73384.0
21	91631.0
22	109878.0
23	131211.0
24	152544.0
25	174978.0
26	220323.5
27	243235.0
28	264731.5
29	286228.0
30	306642.5
31	327057.0
32	343551.5
33	360046.0
34	377440.0
35	394834.0
36	409752.0
37	424670.0
38	434384.5
39	444099.0
40	447977.0
41	451855.0
42	448979.0
43	446103.0
44	434606.5
45	423110.0
46	397103.0
47	371096.0
48	343726.5
49	316357.0
50	289809.5
51	263262.0
52	274492.0
53	285722.0
54	218121.0
55	150520.0
56	128389.0
57	106258.0
58	91607.5
59	76957.0
60	65309.0
61	53661.0
62	44223.5
63	34786.0
64	28417.0
65	22048.0
66	18042.0
67	14036.0
68	11375.5
69	8715.0
70	6951.0
71	5187.0
72	4139.5
73	3092.0
74	2509.0
75	1483.0
76	1040.0
77	815.0
78	590.0
79	463.5
80	337.0
81	237.5
82	138.0
83	96.0
84	54.0
85	43.5
86	33.0
87	25.0
88	17.0
89	12.5
90	8.0
91	6.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04634290658087179
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014975579225290497
20	0.0
21	0.013412910088738447
22	4.8833410517251626E-5
23	0.0
24	8.952791928162797E-4
25	0.0
26	0.09026042043938674
27	0.11469340350151831
28	0.12468797485404916
29	0.7058869490268722
30	0.13816599615681058
31	0.0344601100216739
32	0.12292997207542808
33	0.015773191597072272
34	0.011996741183738148
35	0.017254471716095572
36	0.030276714520696007
37	0.039913174196100326
38	0.008008679324829265
39	0.01621269229172754
40	0.026109596823223866
41	0.012940853787071679
42	0.0165219705583368
43	0.021812256697705725
44	0.01598480304264703
45	0.02383070433241879
46	0.0195496420104064
47	0.009522515050864067
48	0.03188821706776531
49	0.012240908236324408
50	0.014975579225290497
51	0.011394462454025379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6143335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.682548671183916
#Duplication Level	Relative count
1	100.0
2	5.3428996257869805
3	0.8733479628329639
4	0.48807990581108046
5	0.3891373201483586
6	0.3725547638920365
7	0.35210294450923924
8	0.35928871888697883
9	0.35265569638445
10++	2.0203081038952426
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC	83627	1.3612638737754006	TruSeq Adapter, Index 9 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2351005	3.3687503	3.6155396	13
TTTTT	2350115	3.2763827	3.648945	1
GAAGA	658080	2.1875746	16.493671	6
CTCCA	464395	2.160263	21.786844	24
GAGCA	437460	2.1571705	23.30012	9
GGAAG	418480	2.1188166	24.084276	5
TCCAG	416680	1.9901888	22.17462	25
CTGAA	609060	1.9610549	15.647946	19
CGGAA	396505	1.9552159	23.275806	4
CACGT	383445	1.8314484	21.720924	14
TTTCG	571675	1.8206019	14.713645	35
TCGGA	349840	1.7156695	23.037985	3
TCTCG	358355	1.7022493	21.328506	41
GTTTC	525670	1.6740906	14.593053	34
AGAGC	334075	1.647366	22.860254	8
TTCGA	510940	1.6361295	14.582369	36
CACAC	347070	1.6233723	21.67724	12
AGCAC	335480	1.6111659	22.232706	10
GCACA	331015	1.5897224	22.211287	11
TCTGA	496235	1.5890412	15.143603	18
AAGAG	452990	1.5058191	15.777335	7
ATCGG	300790	1.4751208	22.694925	2
CCAGT	303810	1.4510878	21.502731	26
TGAAC	439810	1.4161028	15.088416	20
CTCGT	296225	1.407121	20.928497	42
CGTCT	290330	1.3791186	21.514362	16
GTCAC	288325	1.3771268	21.25543	29
CGTTT	428075	1.3632818	14.392315	33
TCGAT	421170	1.3486685	14.323193	37
ACTCC	289780	1.3479929	21.011105	23
GTCTG	274910	1.340827	21.961676	17
GATCG	268720	1.3178445	22.383629	1
ATGCC	272465	1.3013747	20.980589	47
AACTC	413940	1.2980593	14.609215	22
ACACG	269420	1.2939082	21.859552	13
ACGTC	268775	1.28375	21.586714	15
TCACG	267390	1.2771349	21.044792	30
CAGTC	263320	1.2576954	21.288177	27
ATCTC	403055	1.2570124	14.058207	40
GAACT	389645	1.2545812	14.938742	21
CGATC	262055	1.2516534	20.596292	38
GATCT	357920	1.1461296	14.239628	39
AGTCA	346610	1.116017	14.560917	28
ACGTT	315295	1.009636	14.1872	32
TCGTA	287730	0.9213675	14.118862	43
TATGC	272640	0.8730464	14.033552	46
GTATG	257560	0.8468348	14.381962	45
CGTAT	254835	0.8160312	14.003197	44
>>END_MODULE