##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW177_GTGGCC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5187616	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76916699308507	34.0	31.0	34.0	31.0	34.0
2	32.95634526533961	34.0	31.0	34.0	31.0	34.0
3	33.01947850419152	34.0	33.0	34.0	31.0	34.0
4	36.364635123339895	37.0	37.0	37.0	35.0	37.0
5	36.308347610925715	37.0	37.0	37.0	35.0	37.0
6	36.30613888923158	37.0	37.0	37.0	35.0	37.0
7	36.29505769124006	37.0	37.0	37.0	35.0	37.0
8	36.2804960891477	37.0	37.0	37.0	35.0	37.0
9	38.061325086513726	39.0	38.0	39.0	37.0	39.0
10	38.03511362444714	39.0	38.0	39.0	35.0	39.0
11	38.02791706248111	39.0	38.0	39.0	35.0	39.0
12	38.00697622954359	39.0	38.0	39.0	35.0	39.0
13	37.98125940701856	39.0	38.0	39.0	35.0	39.0
14	39.50923372123149	41.0	39.0	41.0	37.0	41.0
15	39.46831357602413	41.0	39.0	41.0	37.0	41.0
16	39.4418347464423	41.0	39.0	41.0	37.0	41.0
17	39.42224887115777	41.0	39.0	41.0	37.0	41.0
18	39.36264519193402	41.0	39.0	41.0	36.0	41.0
19	39.42406974610303	41.0	39.0	41.0	37.0	41.0
20	39.40251668589194	41.0	39.0	41.0	37.0	41.0
21	39.37000965376003	41.0	39.0	41.0	36.0	41.0
22	39.2959467316008	40.0	39.0	41.0	36.0	41.0
23	39.24112289730003	40.0	39.0	41.0	36.0	41.0
24	39.177024860745284	40.0	39.0	41.0	36.0	41.0
25	39.162190879201546	40.0	39.0	41.0	36.0	41.0
26	39.03730384053099	40.0	39.0	41.0	36.0	41.0
27	38.94607889250091	40.0	39.0	41.0	36.0	41.0
28	38.836476716857995	40.0	38.0	41.0	35.0	41.0
29	38.56464337375781	40.0	38.0	41.0	35.0	41.0
30	38.56594416394737	40.0	38.0	41.0	35.0	41.0
31	38.79879023428103	40.0	38.0	41.0	35.0	41.0
32	38.79042492736548	40.0	38.0	41.0	35.0	41.0
33	38.80912966572699	40.0	38.0	41.0	35.0	41.0
34	38.94856674048349	40.0	39.0	41.0	36.0	41.0
35	38.96943740631535	40.0	39.0	41.0	35.0	41.0
36	38.978578599495414	40.0	39.0	41.0	35.0	41.0
37	38.91691289409239	40.0	39.0	41.0	35.0	41.0
38	38.87556018024464	40.0	38.0	41.0	35.0	41.0
39	38.855215960472016	40.0	38.0	41.0	35.0	41.0
40	38.79597333341558	40.0	38.0	41.0	35.0	41.0
41	38.790289990623826	40.0	38.0	41.0	35.0	41.0
42	38.74832639886992	40.0	38.0	41.0	35.0	41.0
43	38.697741313158105	40.0	38.0	41.0	35.0	41.0
44	38.63292637697162	40.0	38.0	41.0	35.0	41.0
45	38.547360290353026	40.0	38.0	41.0	34.0	41.0
46	38.48658902278041	40.0	38.0	41.0	34.0	41.0
47	38.399007366775024	40.0	38.0	41.0	34.0	41.0
48	38.3440773179819	40.0	38.0	41.0	34.0	41.0
49	38.289117583105615	40.0	38.0	41.0	34.0	41.0
50	38.18237259658387	40.0	38.0	41.0	34.0	41.0
51	37.1986299679853	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	1.0
13	1.0
14	2.0
15	9.0
16	27.0
17	56.0
18	167.0
19	318.0
20	659.0
21	1143.0
22	1970.0
23	3229.0
24	5099.0
25	7632.0
26	11420.0
27	16051.0
28	22120.0
29	29538.0
30	39498.0
31	51489.0
32	66288.0
33	87005.0
34	117560.0
35	166952.0
36	256650.0
37	448641.0
38	1041051.0
39	2811701.0
40	1333.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.40100673697227	27.97057509285443	31.988362412195936	20.64005575797736
2	18.910728935989095	31.09883229599107	30.793701769753195	19.19673699826664
3	18.73039561910519	29.78161837730472	32.41211377249203	19.075872231098064
4	18.741711028726876	29.044593894382313	30.97974098314139	21.23395409374942
5	20.82750535120564	29.77795580860264	30.988878128219206	18.405660711972512
6	20.75267328961897	30.255670427417915	29.74481920018752	19.246837082775595
7	19.29662874044648	31.88996641231733	30.364410164514876	18.448994682721313
8	18.65398286997341	32.122173268029094	30.277800053049415	18.94604380894808
9	20.30716999870461	29.767604232849926	30.681453677373188	19.243772091072277
10	18.682146095624656	31.776272569133873	30.167055541505	19.37452579373647
11	20.4776143800929	29.823872854120275	30.245685108535405	19.452827657251422
12	18.74250137249943	29.881298076033385	30.114237445485557	21.26196310598163
13	18.696314453498488	31.806748996070645	30.164086933188578	19.332849617242296
14	18.659322509607495	30.15290646030855	30.179238401608753	21.008532628475198
15	18.631043623891976	31.941415864242845	30.221377218360036	19.206163293505146
16	18.649202253983333	30.182862416956073	30.239940658676357	20.927994670384237
17	20.447003016414474	30.098989593678482	30.30632953557087	19.147677854336173
18	18.78061136367842	30.01914559597318	32.005337326432794	19.194905713915603
19	18.73102906925862	29.960721281871066	30.21951436730536	21.08873528156495
20	18.80326145959917	29.9204104544361	31.95325174415377	19.32307634181096
21	20.6635473675829	29.90403229398041	30.070780930773193	19.361639407663496
22	18.856869899391164	31.729160369618725	30.1098038096883	19.30416592130181
23	18.847424327475277	31.833736344401743	30.087558523992524	19.231280804130453
24	18.84022937255653	29.985717708749593	30.116743128683467	21.057309790010414
25	18.783695632059118	29.989343852744692	31.964624983807592	19.2623355313886
26	18.797436824539556	30.009573705765025	30.143416504821772	21.049572964873644
27	18.885840564847413	30.02722263156737	30.078286776702246	21.008650026882975
28	18.879766755430385	31.83950114752051	30.0476861311854	19.233045965863703
29	20.705752441315912	30.042653745971908	30.09532299763173	19.15627081508045
30	18.983893633120744	29.9435775282981	31.781622064023967	19.29090677455719
31	18.90964311368008	29.947867504206176	30.059595146384616	21.08289423572913
32	18.947247947231734	31.75029953895705	30.031675472700265	19.27077704111095
33	18.993193291811224	29.9842966610402	30.02709765857336	20.995412388575218
34	20.707784230726816	30.072539767123175	30.059102192657416	19.160573809492597
35	18.97684786732928	30.075137813067794	31.8207442985338	19.127270021069126
36	20.70630330170743	30.076007839501557	30.073019048816402	19.144669809974616
37	20.712138121032357	30.136327197616293	30.011345011579316	19.140189669772035
38	18.990816756359205	30.071879340641754	30.045313769660304	20.89199013333873
39	18.96399245843085	30.089812075803607	30.038238046055703	20.907957419709838
40	18.968835376274072	31.840104368623624	30.02934885493961	19.1617114001627
41	18.97088150556474	30.05727457246502	31.734639895861612	19.23720402610863
42	18.988244761755478	30.016393825063254	30.035712433452506	20.959648979728765
43	18.940798520464313	30.021062405957633	31.81909853519489	19.21904053838317
44	18.99775312786426	30.038158652231466	30.07396130845309	20.890126911451183
45	20.713301402157928	30.109327094921817	30.0944997486678	19.08287175425245
46	18.920854778800393	30.157434391090128	31.83517927430371	19.08653155580577
47	18.95498977460957	31.94281647043582	30.077993664267886	19.024200090686723
48	19.02345121642929	30.10836765518881	31.79015946333531	19.078021665046585
49	20.765834093571357	30.061160923945607	30.039356294963614	19.133648687519425
50	19.044370903699647	30.070842679895005	29.981790471857167	20.902995944548177
51	19.0639978500171	30.042656493576846	30.004195088584616	20.889150567821435
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.04106249494963
2	38.107465934255735
3	37.80626785020325
4	39.97566512247629
5	39.23316606317815
6	39.99951037239457
7	37.74562342316779
8	37.600026678921495
9	39.55094208977689
10	38.05667188936113
11	39.93044203734432
12	40.00446447848106
13	38.02916407074078
14	39.6678551380827
15	37.837206917397125
16	39.57719692436757
17	39.59468087075065
18	37.97551707759402
19	39.81976435082357
20	38.12633780141012
21	40.0251867752464
22	38.16103582069297
23	38.07870513160573
24	39.89753916256694
25	38.04603116344772
26	39.8470097894132
27	39.89449059173039
28	38.112812721294084
29	39.86202325639636
30	38.27480040767794
31	39.992537349409204
32	38.218024988342684
33	39.98860568038644
34	39.86835804021941
35	38.104117888398406
36	39.850973111682045
37	39.85232779080439
38	39.88280688969794
39	39.87194987814069
40	38.130546776436766
41	38.20808553167337
42	39.94789374148424
43	38.15983905884748
44	39.88788003931544
45	39.79617315641038
46	38.00738633460617
47	37.97918986529629
48	38.10147288147588
49	39.899482781090775
50	39.94736684824783
51	39.953148417838534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	20.5
2	24.0
3	52.0
4	80.0
5	180.0
6	280.0
7	614.5
8	949.0
9	1841.5
10	2734.0
11	4609.5
12	6485.0
13	10211.0
14	13937.0
15	20210.0
16	26483.0
17	36300.0
18	46117.0
19	60305.0
20	74493.0
21	92492.0
22	110491.0
23	130753.0
24	151015.0
25	171835.5
26	212708.5
27	232761.0
28	250600.0
29	268439.0
30	283499.5
31	298560.0
32	309700.0
33	320840.0
34	332800.0
35	344760.0
36	352540.5
37	360321.0
38	364658.0
39	368995.0
40	368003.5
41	367012.0
42	360442.5
43	353873.0
44	341834.0
45	329795.0
46	307810.5
47	285826.0
48	263332.5
49	240839.0
50	217575.5
51	194312.0
52	171649.5
53	148987.0
54	131015.0
55	113043.0
56	139677.5
57	166312.0
58	112772.5
59	59233.0
60	49273.5
61	39314.0
62	32514.5
63	25715.0
64	20943.0
65	16171.0
66	13400.5
67	10630.0
68	8575.5
69	6521.0
70	5231.5
71	3942.0
72	3153.5
73	2365.0
74	1910.5
75	1123.0
76	790.0
77	643.0
78	496.0
79	392.5
80	289.0
81	206.5
82	124.0
83	93.5
84	63.0
85	49.5
86	36.0
87	27.5
88	19.0
89	11.5
90	4.0
91	6.0
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04528091516411392
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0014264741260725544
20	0.0
21	0.014785211549968232
22	0.0
23	0.0
24	0.0014071974486931955
25	0.0
26	0.0900220833616058
27	0.11369384318345845
28	0.12423818570996774
29	0.7055842221166717
30	0.136016235588756
31	0.03452452918643169
32	0.12182860103754788
33	0.015826152128453612
34	0.012876820489411708
35	0.01696347609383578
36	0.030302936840352096
37	0.040037658916928315
38	0.008693781498090837
39	0.017291179609284883
40	0.02665964481565328
41	0.012953927198929141
42	0.01748394638307847
43	0.02166698537439934
44	0.016385175772455015
45	0.024153676756336628
46	0.01948872083053179
47	0.010178085656301468
48	0.031980007772356316
49	0.012414180232307094
50	0.014862318259485668
51	0.011527453072856589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5187616.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.140015097280065
#Duplication Level	Relative count
1	100.0
2	4.453320615237868
3	0.878524122830788
4	0.5788178674788905
5	0.5126982234410397
6	0.3902142926823981
7	0.29753839817032857
8	0.22599911117855556
9	0.1831839318425702
10++	0.8725625155814735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC	84964	1.6378236168598446	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2258855	3.579368	3.7442493	14
TTTTT	2292765	3.538884	3.894554	1
GTGGC	256660	2.4262426	41.62693	34
GAGCA	369335	2.2299025	28.28038	9
CTCCA	384755	2.2065077	26.540287	24
GGAAG	351890	2.1742194	29.099272	5
CGGAA	355225	2.1447117	28.468878	4
CGTGG	224665	2.1237893	41.50968	33
GAAGA	540365	2.121245	19.073122	6
TCCAG	349660	2.052098	27.06831	25
CACGT	346865	2.0356948	26.62879	14
CTGAA	532665	2.0325654	18.320847	19
GGCCA	218750	2.0313058	40.34952	36
TGGCC	218725	2.0204284	39.943413	35
TCGGA	301330	1.8097789	27.995398	3
TCTCG	306175	1.7874738	26.085165	41
CCATC	303685	1.7415845	25.384886	38
AGAGC	284835	1.719724	27.784332	8
CACAC	296925	1.7117889	26.470497	12
AGCAC	289555	1.7083055	27.20811	10
GCACA	288885	1.7043526	27.138865	11
TCTGA	430450	1.6339202	17.8728	18
ATCGG	265135	1.592393	27.631659	2
CCAGT	262330	1.5395726	26.463404	26
ACGTG	249690	1.4996307	26.53461	32
AAGAG	379390	1.4893252	18.50364	7
GTCAC	252845	1.4839065	26.188694	29
CTCGT	254090	1.4833974	25.687796	42
TGAAC	384320	1.4665043	17.806591	20
CGTCT	249805	1.4583812	26.38273	16
GTCTG	242810	1.4506663	27.001848	17
GCCAT	245180	1.4389218	25.69921	37
ACTCC	247595	1.4199173	25.856136	23
ACACG	240440	1.4185387	26.742779	13
ATGCC	237975	1.396637	25.794592	47
GATCG	229505	1.3784003	27.281582	1
TCACG	232815	1.3663538	26.041458	30
ACGTC	232375	1.3637713	26.458366	15
CAGTC	227365	1.3343685	26.215055	27
AACTC	357380	1.3325685	17.269484	22
CATCT	350745	1.300974	16.70005	39
GAACT	337640	1.2883809	17.552158	21
ATCTC	342980	1.2721722	16.71239	40
AGTCA	304710	1.1627252	17.285538	28
TCGTA	260300	0.9880576	16.689371	43
TATGC	241735	0.9175879	16.646488	46
GTATG	233190	0.905835	17.065222	45
CGTAT	232695	0.8832735	16.653181	44
>>END_MODULE