FastQCFastQC Report
Wed 23 Jul 2014
LW176_GTGAAA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW176_GTGAAA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1447683
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG 39052 2.697551881178407 TruSeq Adapter, Index 1 (97% over 35bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 553035 3.4309046 3.9439714 1
AAAAA 550695 3.358978 3.6639688 13
CACGT 139925 2.8066747 45.46053 14
GAGCA 128475 2.6251183 47.345295 9
CTCCA 132990 2.6098187 44.26523 24
GGAAG 121490 2.5373256 48.339043 5
TGAAA 270800 2.498024 21.643097 35
TCCAG 121375 2.4345913 45.307503 25
GAAGA 175605 2.4332833 32.705692 6
CGGAA 117920 2.409449 47.24937 4
CTGAA 166070 2.258986 31.57199 19
TCGGA 105300 2.1588905 47.140278 3
TCTCG 107240 2.1583686 43.292557 43
AGAGC 103700 2.118893 46.908825 8
AGCAC 105680 2.1126018 45.841663 10
CACAC 105350 2.0604112 44.58754 12
GCACA 103055 2.0601265 45.719658 11
ATCGG 96130 1.9708848 46.861145 2
GAAAC 143480 1.9450991 29.59629 36
CCAGT 95550 1.9165825 44.60098 26
CTCGT 94815 1.9082966 43.186436 44
TCTGA 139590 1.9052354 31.426025 18
CGTCT 92850 1.868748 45.190456 16
ACGTG 91060 1.866938 44.671593 32
GTGAA 134245 1.8664911 30.658655 34
GTCAC 91740 1.8401598 44.496662 29
GTCTG 89110 1.8331614 46.19264 17
GATCG 88425 1.8129146 46.58945 1
ACTCC 92060 1.8066015 43.506233 23
AAGAG 129760 1.7980288 32.16575 7
ACACG 88920 1.77756 45.23632 13
ACGTC 88470 1.7745686 45.028126 15
CGTGA 86455 1.772525 44.432144 33
TCACG 87600 1.757118 44.211277 30
CAGTC 86320 1.731443 44.387596 27
TGAAC 126640 1.722635 31.06101 20
AAACG 124170 1.6833215 29.266706 37
CGATC 83240 1.6696632 41.58654 40
AACTC 119440 1.5895226 30.113577 22
GAACT 115165 1.566545 30.728876 21
ATCTC 116960 1.561803 28.707554 42
AACGA 113560 1.539486 28.974148 38
ACGAT 106445 1.4479301 28.90722 39
GATCT 105570 1.4409035 29.068768 41
AGTCA 105690 1.4376605 30.435863 28
TCGTA 95560 1.3042791 29.562862 45
GTATG 85635 1.1946787 29.982744 47
CGTAT 86440 1.1798021 29.47229 46
Produced by FastQC (version 0.10.1)