##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW176_GTGAAA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1447683	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78407358517023	34.0	31.0	34.0	31.0	34.0
2	32.95805642533621	34.0	31.0	34.0	31.0	34.0
3	33.026780724785745	34.0	33.0	34.0	31.0	34.0
4	36.37183969142416	37.0	37.0	37.0	35.0	37.0
5	36.315790128087436	37.0	37.0	37.0	35.0	37.0
6	36.31298288368379	37.0	37.0	37.0	35.0	37.0
7	36.28177370322094	37.0	37.0	37.0	35.0	37.0
8	36.284844126787426	37.0	37.0	37.0	35.0	37.0
9	38.071119160755494	39.0	38.0	39.0	37.0	39.0
10	38.05688814471124	39.0	38.0	39.0	37.0	39.0
11	38.037856353911735	39.0	38.0	39.0	35.0	39.0
12	38.01460264436344	39.0	38.0	39.0	35.0	39.0
13	37.99811491880474	39.0	38.0	39.0	35.0	39.0
14	39.523923400357674	41.0	39.0	41.0	37.0	41.0
15	39.47797204222195	41.0	39.0	41.0	37.0	41.0
16	39.46012904758846	41.0	39.0	41.0	37.0	41.0
17	39.43173540063674	41.0	39.0	41.0	37.0	41.0
18	39.35726882197277	41.0	39.0	41.0	36.0	41.0
19	39.427656468992176	41.0	39.0	41.0	37.0	41.0
20	39.4075263714501	41.0	39.0	41.0	37.0	41.0
21	39.37340633273997	41.0	39.0	41.0	36.0	41.0
22	39.29211091102127	40.0	39.0	41.0	36.0	41.0
23	39.23495544259344	40.0	39.0	41.0	36.0	41.0
24	39.18380266950707	40.0	39.0	41.0	36.0	41.0
25	39.15271851641554	40.0	39.0	41.0	36.0	41.0
26	39.03635464393793	40.0	39.0	41.0	36.0	41.0
27	38.95539009575992	40.0	39.0	41.0	36.0	41.0
28	38.82121085900712	40.0	38.0	41.0	35.0	41.0
29	38.54135746568828	40.0	38.0	41.0	35.0	41.0
30	38.5058911377698	40.0	38.0	41.0	35.0	41.0
31	38.76441872979098	40.0	38.0	41.0	35.0	41.0
32	38.76972997541589	40.0	38.0	41.0	35.0	41.0
33	38.80192003359851	40.0	38.0	41.0	35.0	41.0
34	38.94064791808704	40.0	39.0	41.0	36.0	41.0
35	38.91938359433661	40.0	39.0	41.0	35.0	41.0
36	38.95600832502696	40.0	39.0	41.0	35.0	41.0
37	38.92554101968456	40.0	39.0	41.0	35.0	41.0
38	38.89544948721509	40.0	38.0	41.0	35.0	41.0
39	38.86216802987947	40.0	38.0	41.0	35.0	41.0
40	38.76480831784306	40.0	38.0	41.0	35.0	41.0
41	38.7357771003735	40.0	38.0	41.0	35.0	41.0
42	38.62874399989501	40.0	38.0	41.0	35.0	41.0
43	38.61388646547621	40.0	38.0	41.0	35.0	41.0
44	38.57527856581862	40.0	38.0	41.0	35.0	41.0
45	38.50485569009238	40.0	38.0	41.0	34.0	41.0
46	38.500771923135105	40.0	38.0	41.0	34.0	41.0
47	38.38982912695666	40.0	38.0	41.0	34.0	41.0
48	38.2372770834499	40.0	38.0	41.0	34.0	41.0
49	38.19301946627819	40.0	38.0	41.0	34.0	41.0
50	38.11091723809702	40.0	38.0	41.0	33.0	41.0
51	37.09604243470428	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	2.0
13	2.0
14	1.0
15	2.0
16	9.0
17	20.0
18	55.0
19	99.0
20	201.0
21	362.0
22	571.0
23	995.0
24	1499.0
25	2189.0
26	3151.0
27	4696.0
28	6315.0
29	8652.0
30	11425.0
31	14504.0
32	18790.0
33	24364.0
34	32974.0
35	46365.0
36	72087.0
37	126417.0
38	289906.0
39	781625.0
40	401.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.504867680769706	26.693149172110715	31.957721979304672	20.84426116781491
2	18.787262128518467	31.839843391129136	30.331363979545245	19.041530500807152
3	18.834855420696382	29.179247114181766	32.891662055850624	19.094235409271228
4	18.96223137247588	28.22717404293619	29.914007417369685	22.89658716721824
5	22.3821098956056	28.827581728872964	30.003253474690244	18.78705490083119
6	22.41160530309467	29.395523743803032	28.595832098601697	19.597038854500607
7	19.533143650923577	32.466016386183995	29.239481295283564	18.761358667608864
8	18.955738238274538	32.75247412589635	29.18746714577708	19.104320490052036
9	21.957224060792313	28.993709258173233	29.51854791414971	19.53051876688474
10	18.85923921189929	32.3510740956411	29.23796162557687	19.551725066882735
11	22.1404824122408	28.94784286338929	29.300475311238717	19.61119941313119
12	18.84348990766625	29.060712877059412	29.20003895880521	22.895758256469133
13	18.84473327378991	32.43009692038934	29.261309278343393	19.463860527477355
14	18.8758865027772	29.271946966290272	29.25626673795299	22.59589979297954
15	18.765917676728954	32.509188821033334	29.301856829153895	19.423036673083818
16	18.849292282910003	29.3227177496731	29.220968955220165	22.607021012196732
17	22.168734453606213	29.21482120049762	29.261378354239152	19.355065991657014
18	18.996493016772316	29.05698277868843	32.45655298846501	19.489971216074238
19	18.96667725847229	28.97911111725129	29.264468176229226	22.789743448047194
20	19.09009085552569	28.962625105081706	32.39569712430138	19.551586915091217
21	22.292735330590645	28.95615498606203	29.169490530126392	19.58161915322093
22	19.053964162043762	32.156970828558464	29.210400343169052	19.578664666228725
23	19.09154144933663	32.21112633083348	29.224491825903876	19.47284039392602
24	19.077878039126563	28.995116950722576	29.119110067895733	22.80789494225513
25	19.072407426211402	29.04088809497659	32.33124931355829	19.55545516525372
26	19.0336059209148	29.131285250331374	29.129487513681813	22.705621315072015
27	19.181221839155977	29.008911188953864	29.10794745268632	22.701919519203837
28	19.177969442734522	32.25195565115748	29.029194707465017	19.540880198642977
29	22.404996622117583	29.016949246881808	29.01660137021661	19.561452760784004
30	19.24953755010162	28.928264578067214	32.201801098811394	19.620396773019767
31	19.189164910341013	28.944615278305637	29.031682963065336	22.834536848288014
32	19.185993432173497	32.12646524225431	29.01919719802246	19.668344127549737
33	19.292439892694272	29.01310094351941	28.957624185379423	22.736834978406897
34	22.351249815197853	29.10782359028777	29.01055202992206	19.53037456459232
35	19.273877913383515	29.09808175264178	32.130453256322475	19.497587077652227
36	22.271281866311778	29.102319070105544	29.011593277584996	19.614805785997685
37	19.244515822294954	32.309104177262775	28.94133809513279	19.50504190530948
38	19.208495875510252	32.3563647567342	28.97822769559808	19.456911672157467
39	19.221987092994418	32.23269364826372	28.978099819611437	19.567219439130433
40	19.265930800988063	29.125839940577986	29.03345943238154	22.57476982605241
41	22.431815135734134	29.055823227138262	28.94113973285655	19.571221904271063
42	19.26535592246076	32.129676072354854	28.963564185151252	19.641403820033133
43	19.18852459582724	29.040604861749735	32.16316288029273	19.6077076621303
44	19.331319632614765	28.931725320536295	29.146590687693536	22.59036435915541
45	19.23025634399746	28.96401814949496	32.210033351458875	19.5956921550487
46	19.213116974009385	29.157244645079096	29.091471852768436	22.538166528143087
47	22.387568901805903	29.085456546353754	29.0674258444206	19.45954870741974
48	19.335831961713495	29.113512079237474	32.10516350952257	19.445492449526462
49	19.328417291534546	32.13845054873693	29.000570636667483	19.532561523061045
50	19.362139576528328	29.062208110184436	32.021176347045596	19.554475966241647
51	22.453064032983676	28.932009859730957	29.06043782953277	19.5544882777526
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.34912884858461
2	37.82879262932562
3	37.92909082996761
4	41.85881853969412
5	41.16916479643679
6	42.00864415759528
7	38.294502318532444
8	38.060058728326574
9	41.48774282767705
10	38.410964278782025
11	41.75168182537199
12	41.739248164135375
13	38.308593801267264
14	41.471786295756736
15	38.18895434981277
16	41.45631329510673
17	41.52380044526323
18	38.48646423284656
19	41.756420706519485
20	38.64167777061691
21	41.87435448381158
22	38.63262882827249
23	38.564381843262645
24	41.88577298138169
25	38.627862591465124
26	41.73922723598682
27	41.883141358359815
28	38.718849641377496
29	41.96644938290158
30	38.869934323121385
31	42.02370175862903
32	38.854337559723234
33	42.029274871101165
34	41.881624379790175
35	38.77146499103574
36	41.88608765230947
37	38.74955772760443
38	38.66540754766772
39	38.78920653212485
40	41.84070062704047
41	42.003037040005196
42	38.9067597424939
43	38.796232257957534
44	41.92168399177017
45	38.82594849904616
46	41.75128350215247
47	41.84711760922564
48	38.78132441123996
49	38.86097881459559
50	38.916615542769975
51	42.00755231073627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	10.5
4	14.0
5	48.5
6	83.0
7	158.5
8	234.0
9	414.5
10	595.0
11	1013.5
12	1432.0
13	2306.0
14	3180.0
15	4542.5
16	5905.0
17	8298.0
18	10691.0
19	13830.0
20	16969.0
21	21226.5
22	25484.0
23	30454.5
24	35425.0
25	40551.0
26	50892.5
27	56108.0
28	60718.0
29	65328.0
30	70089.5
31	74851.0
32	78514.5
33	82178.0
34	85897.0
35	89616.0
36	93182.0
37	96748.0
38	98987.0
39	101226.0
40	102072.0
41	102918.0
42	102626.0
43	102334.0
44	100074.5
45	97815.0
46	92108.5
47	86402.0
48	82138.5
49	77875.0
50	89886.0
51	101897.0
52	75610.0
53	49323.0
54	42241.5
55	35160.0
56	30490.0
57	25820.0
58	22162.5
59	18505.0
60	15830.5
61	13156.0
62	11026.5
63	8897.0
64	7294.5
65	5692.0
66	4809.0
67	3926.0
68	3230.0
69	2534.0
70	2033.5
71	1533.0
72	1215.5
73	898.0
74	711.5
75	420.5
76	316.0
77	257.5
78	199.0
79	156.0
80	113.0
81	75.5
82	38.0
83	30.0
84	22.0
85	18.0
86	14.0
87	10.5
88	7.0
89	6.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04766236807367359
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0015887456024557863
20	0.0
21	0.013677027360271552
22	0.0
23	0.0
24	0.001519669706696839
25	0.0
26	0.09808777197770507
27	0.12074466578663974
28	0.12937915275650816
29	0.7178367087269796
30	0.14650997490472706
31	0.03681745243951887
32	0.12758317946677553
33	0.01588745602455786
34	0.013055344298441027
35	0.01851234006339786
36	0.03212029152791046
37	0.04220537230871675
38	0.007598348533484196
39	0.0174071257312547
40	0.02818296546965047
41	0.015265772962727336
42	0.018028808793085226
43	0.023762108141077848
44	0.018305112376121015
45	0.025558081430810477
46	0.019064947229469435
47	0.010499536155359979
48	0.03377811302612519
49	0.012226433549333659
50	0.015127621171209443
51	0.012364585340851554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1447683.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	9.366774226028094
#Duplication Level	Relative count
1	100.0
2	3.049235310610429
3	1.15748210610958
4	0.6797826811475366
5	0.3946442646973248
6	0.19838015986795818
7	0.09680951801556359
8	0.056075458522676175
9	0.03438589437711275
10++	0.13595653622950732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG	39052	2.697551881178407	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	553035	3.4309046	3.9439714	1
AAAAA	550695	3.358978	3.6639688	13
CACGT	139925	2.8066747	45.46053	14
GAGCA	128475	2.6251183	47.345295	9
CTCCA	132990	2.6098187	44.26523	24
GGAAG	121490	2.5373256	48.339043	5
TGAAA	270800	2.498024	21.643097	35
TCCAG	121375	2.4345913	45.307503	25
GAAGA	175605	2.4332833	32.705692	6
CGGAA	117920	2.409449	47.24937	4
CTGAA	166070	2.258986	31.57199	19
TCGGA	105300	2.1588905	47.140278	3
TCTCG	107240	2.1583686	43.292557	43
AGAGC	103700	2.118893	46.908825	8
AGCAC	105680	2.1126018	45.841663	10
CACAC	105350	2.0604112	44.58754	12
GCACA	103055	2.0601265	45.719658	11
ATCGG	96130	1.9708848	46.861145	2
GAAAC	143480	1.9450991	29.59629	36
CCAGT	95550	1.9165825	44.60098	26
CTCGT	94815	1.9082966	43.186436	44
TCTGA	139590	1.9052354	31.426025	18
CGTCT	92850	1.868748	45.190456	16
ACGTG	91060	1.866938	44.671593	32
GTGAA	134245	1.8664911	30.658655	34
GTCAC	91740	1.8401598	44.496662	29
GTCTG	89110	1.8331614	46.19264	17
GATCG	88425	1.8129146	46.58945	1
ACTCC	92060	1.8066015	43.506233	23
AAGAG	129760	1.7980288	32.16575	7
ACACG	88920	1.77756	45.23632	13
ACGTC	88470	1.7745686	45.028126	15
CGTGA	86455	1.772525	44.432144	33
TCACG	87600	1.757118	44.211277	30
CAGTC	86320	1.731443	44.387596	27
TGAAC	126640	1.722635	31.06101	20
AAACG	124170	1.6833215	29.266706	37
CGATC	83240	1.6696632	41.58654	40
AACTC	119440	1.5895226	30.113577	22
GAACT	115165	1.566545	30.728876	21
ATCTC	116960	1.561803	28.707554	42
AACGA	113560	1.539486	28.974148	38
ACGAT	106445	1.4479301	28.90722	39
GATCT	105570	1.4409035	29.068768	41
AGTCA	105690	1.4376605	30.435863	28
TCGTA	95560	1.3042791	29.562862	45
GTATG	85635	1.1946787	29.982744	47
CGTAT	86440	1.1798021	29.47229	46
>>END_MODULE