FastQCFastQC Report
Wed 23 Jul 2014
LW175_GTCCGC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW175_GTCCGC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4428898
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG 142386 3.214930666725673 TruSeq Adapter, Index 1 (97% over 36bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1586135 3.317036 3.77247 1
AAAAA 1592425 3.2664635 3.4886794 12
GCACA 499680 3.2001812 51.01777 11
CACGT 473760 3.045925 50.863636 14
ACGTC 456850 2.9372065 50.62336 15
GAGCA 416225 2.7540271 53.233295 9
GGAAG 400065 2.7348156 55.708115 5
CTCCA 429940 2.6755388 48.953407 24
GAAGA 567030 2.5950394 37.94139 6
TCCAG 397630 2.556466 50.608356 25
TCCGC 271635 2.5249264 70.41586 35
CGGAA 379895 2.5136428 53.654217 4
CCGCA 260335 2.4105563 70.45051 36
CTGAA 525215 2.335586 36.139244 19
TCGGA 349045 2.3184607 53.557907 3
GTCCG 240190 2.306616 72.71257 34
TCTCG 355890 2.2969675 50.386574 43
CGTCC 246280 2.2892442 70.527885 33
AGAGC 341145 2.2572467 52.91953 8
AGCAC 344090 2.2037113 51.11399 10
CGCAC 236385 2.1887927 70.13543 37
CACAC 347155 2.1520314 49.15009 12
ATCGG 313690 2.0836225 53.25541 2
CTCGT 315250 2.034671 49.9994 44
CCAGT 314655 2.022998 49.968506 26
TCTGA 448290 2.0012255 35.949013 18
CGTCT 308650 1.9920737 50.840576 16
GTCTG 297815 1.9858347 52.619473 17
GTCAC 302910 1.9474865 50.485188 29
AAGAG 425380 1.9467715 37.27315 7
GATCG 291365 1.9353327 52.954742 1
ACTCC 305345 1.9001777 48.344486 23
TCACG 292505 1.8805901 50.151455 30
ACACG 292835 1.8754505 50.641907 13
CAGTC 288200 1.8529122 50.216183 27
TGAAC 410360 1.8248355 35.543682 20
CATCT 396530 1.7133875 33.56382 41
AACTC 390405 1.6804154 34.041065 22
ATCTC 387190 1.6730299 33.75738 42
GAACT 374460 1.6651913 35.34218 21
CACAT 383615 1.6511893 33.316654 39
ACATC 365060 1.5713233 33.282623 40
AGTCA 343745 1.5286044 35.044098 28
TCGTA 314240 1.4028087 34.613483 45
GTATG 281650 1.2989849 35.452656 47
CGTAT 285825 1.2759604 34.401783 46
Produced by FastQC (version 0.10.1)