##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW175_GTCCGC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4428898	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7297156087135	34.0	31.0	34.0	31.0	34.0
2	32.91228337161976	34.0	31.0	34.0	31.0	34.0
3	32.97832440485195	34.0	31.0	34.0	31.0	34.0
4	36.33735457443364	37.0	37.0	37.0	35.0	37.0
5	36.277970727706986	37.0	37.0	37.0	35.0	37.0
6	36.272979869936044	37.0	37.0	37.0	35.0	37.0
7	36.2487417863315	37.0	37.0	37.0	35.0	37.0
8	36.24226681219572	37.0	37.0	37.0	35.0	37.0
9	38.003854909279916	39.0	38.0	39.0	35.0	39.0
10	37.98076090259925	39.0	38.0	39.0	35.0	39.0
11	37.97019281094304	39.0	38.0	39.0	35.0	39.0
12	37.94416105315589	39.0	38.0	39.0	35.0	39.0
13	37.92942172070795	39.0	38.0	39.0	35.0	39.0
14	39.44388807328595	41.0	39.0	41.0	37.0	41.0
15	39.38825594989995	41.0	39.0	41.0	36.0	41.0
16	39.37876171453937	41.0	39.0	41.0	36.0	41.0
17	39.350646368464574	41.0	39.0	41.0	36.0	41.0
18	39.2756468990706	40.0	39.0	41.0	36.0	41.0
19	39.3484180489142	40.0	39.0	41.0	36.0	41.0
20	39.32858670486428	40.0	39.0	41.0	36.0	41.0
21	39.29422849656957	40.0	39.0	41.0	36.0	41.0
22	39.22359196350875	40.0	39.0	41.0	36.0	41.0
23	39.16703410193687	40.0	39.0	41.0	36.0	41.0
24	39.0983544890851	40.0	39.0	41.0	36.0	41.0
25	39.079317021977026	40.0	39.0	41.0	36.0	41.0
26	38.95124091816971	40.0	38.0	41.0	36.0	41.0
27	38.86413234172473	40.0	38.0	41.0	35.0	41.0
28	38.71933943838851	40.0	38.0	41.0	35.0	41.0
29	38.46139536290969	40.0	38.0	41.0	35.0	41.0
30	38.43762940577995	40.0	38.0	41.0	34.0	41.0
31	38.68766812873089	40.0	38.0	41.0	35.0	41.0
32	38.674568933400586	40.0	38.0	41.0	35.0	41.0
33	38.708928496434105	40.0	38.0	41.0	35.0	41.0
34	38.83700098760459	40.0	38.0	41.0	35.0	41.0
35	38.8266720073481	40.0	38.0	41.0	35.0	41.0
36	38.864058960039266	40.0	38.0	41.0	35.0	41.0
37	38.82331541615996	40.0	38.0	41.0	35.0	41.0
38	38.78360734431003	40.0	38.0	41.0	35.0	41.0
39	38.73997052991512	40.0	38.0	41.0	35.0	41.0
40	38.68371183983013	40.0	38.0	41.0	35.0	41.0
41	38.66607314054196	40.0	38.0	41.0	35.0	41.0
42	38.56680126749363	40.0	38.0	41.0	34.0	41.0
43	38.563250045496645	40.0	38.0	41.0	34.0	41.0
44	38.5073433617121	40.0	38.0	41.0	34.0	41.0
45	38.426482163283055	40.0	38.0	41.0	34.0	41.0
46	38.417136723401626	40.0	38.0	41.0	34.0	41.0
47	38.28977388957705	40.0	38.0	41.0	34.0	41.0
48	38.117818473128075	40.0	38.0	41.0	33.0	41.0
49	38.06519703095443	40.0	38.0	41.0	33.0	41.0
50	37.997022058308865	40.0	37.0	41.0	33.0	41.0
51	36.96756823029115	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	3.0
14	5.0
15	4.0
16	18.0
17	62.0
18	149.0
19	298.0
20	600.0
21	1018.0
22	1806.0
23	3008.0
24	4597.0
25	7231.0
26	10278.0
27	15021.0
28	20640.0
29	27656.0
30	36411.0
31	47271.0
32	61405.0
33	79697.0
34	106043.0
35	151636.0
36	231475.0
37	404795.0
38	929428.0
39	2287475.0
40	865.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.387323062354607	27.422008736702146	30.754022845179474	20.43664535576377
2	18.7052174152577	32.426870070161925	29.91622295207521	18.951689562505162
3	18.644050958048705	28.71012156974489	33.507477480854156	19.13834999135225
4	18.979077865419345	28.0071701809344	29.8027861558338	23.21096579781246
5	22.996939645031336	28.745073831007172	29.588760003052677	18.669226520908815
6	23.073640440579123	29.161633435676325	28.389883894368307	19.374842229376245
7	19.408575225710774	32.93085548594707	28.967454206441424	18.693115081900736
8	18.83673997459413	33.12887314180638	28.951942447082775	19.082444436516713
9	22.48642890398469	28.931463312092536	29.258723050293778	19.323384733629
10	18.791469119406226	32.8419394621416	28.97260221391416	19.393989204538016
11	22.52623564597785	28.957361402317233	29.072581937989995	19.443821013714924
12	18.808945250037368	28.878515603655806	29.086309957917294	23.226229188389528
13	18.80693572080459	32.75862302541174	29.071227199181376	19.363214054602295
14	18.824073166733577	29.09662855184292	29.03584593729637	23.04345234412714
15	18.75303969520183	32.89039395352975	29.03336224948057	19.32320410178785
16	18.866724860224824	29.04503558221481	29.02342749821739	23.06481205934298
17	22.65014908900589	29.0237436039394	29.038284467151875	19.287822839902837
18	18.951260561882435	28.901297794620696	32.800981192161125	19.34646045133575
19	18.90786964991966	28.861757961277203	29.027129623023058	23.20324276578008
20	18.985648348641128	28.80612739331545	32.71468433005231	19.493539927991115
21	22.804755005482864	28.798236993332043	28.86731473435668	19.529693266828414
22	19.045364373136948	32.57348222829447	28.871406721407194	19.509746677161385
23	19.062597512970495	32.616014186824806	28.860836262203378	19.460552038001328
24	19.041988231734578	28.77939243958942	28.898339527553368	23.280279801122635
25	19.041892588178822	28.81658146112193	32.59971216316113	19.541813787538118
26	19.08807963692591	28.828768767167123	28.84149227030235	23.241659325604616
27	19.13902982513959	28.820163535779724	28.794101066323446	23.246705572757236
28	19.169206722612326	32.58264629558	28.715943789690375	19.5322031921173
29	22.92639206816489	28.878974398150547	28.749787416706003	19.44484611697856
30	19.228892672151645	28.70185101028942	32.46783284610636	19.60142347145257
31	19.193258406663897	28.7386164465244	28.728881701389515	23.339243445422188
32	19.193599578983918	32.45958480725131	28.765295289973018	19.58152032379175
33	19.269704686019445	28.743928733698926	28.74438038081078	23.24198619947085
34	22.92957916954866	28.823517855243573	28.75195642272325	19.494946552484517
35	19.284682408898924	28.848401278101303	32.42081139875943	19.44610491424034
36	19.226668178406705	28.821752975504168	28.714131840746017	23.23744700534311
37	19.223631497821636	28.862936553685692	28.715935395385227	23.197496553107445
38	22.96465987058926	28.82772745388712	28.690706971944447	19.516905703579173
39	19.299774085706517	28.78517095325715	28.70177416338996	23.21328079764637
40	19.264084173839358	32.53768415793978	28.660353959956087	19.53787770826478
41	19.236170623562526	28.877131298487356	28.69494133812971	23.191756739820406
42	19.286319658931472	32.495047085712685	28.61408052615498	19.604552729200865
43	19.225844304366916	28.766668834676594	32.43514488629691	19.572341974659576
44	19.24419275559768	28.811854156264626	28.784326159117242	23.159626929020448
45	19.258423642093874	28.783420452068885	32.41187344239266	19.546282463444573
46	19.214671012843866	28.8442733755696	28.775033840653318	23.166021770933217
47	23.003001470476658	28.87574020615577	28.693872592410607	19.427385730956967
48	19.33169690993332	28.808535300173325	32.36626552610907	19.493502263784286
49	19.349035969312524	32.44509399135486	28.754562045855987	19.45130799347663
50	19.399573830789688	28.82490931032489	32.32992072782981	19.445596131055616
51	23.065324908353276	28.695800622253813	28.699639464928563	19.53923500446435
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.823968418118376
2	37.656906977762866
3	37.78240094940096
4	42.190043663231805
5	41.66616616594015
6	42.448482669955375
7	38.101690307611506
8	37.91918441111085
9	41.80981363761369
10	38.18545832394424
11	41.97005665969277
12	42.0351744384269
13	38.170149775406884
14	41.867525510860716
15	38.07624379698968
16	41.931536919567804
17	41.93797192890872
18	38.29772101321819
19	42.11111241569974
20	38.47918827663225
21	42.33444827231128
22	38.555111050298336
23	38.523149550971816
24	42.32226803285721
25	38.58370637571694
26	42.32973896253053
27	42.38573539789683
28	38.70140991472963
29	42.37123818514345
30	38.830316143604215
31	42.53250185208608
32	38.77511990277567
33	42.511690885490296
34	42.424525722033174
35	38.73078732313927
36	42.46411518374982
37	42.42112805092908
38	42.481565574168435
39	42.51305488335289
40	38.80196188210414
41	42.427927363382935
42	38.89087238813234
43	38.798186279026496
44	42.40381968461813
45	38.80470610553845
46	42.38069278377708
47	42.430387201433625
48	38.82519917371761
49	38.80034396278915
50	38.8451699618453
51	42.60455991281763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	34.5
4	54.0
5	123.5
6	193.0
7	378.5
8	564.0
9	1098.5
10	1633.0
11	2827.0
12	4021.0
13	6392.0
14	8763.0
15	12758.5
16	16754.0
17	23328.0
18	29902.0
19	39728.5
20	49555.0
21	61829.5
22	74104.0
23	88766.5
24	103429.0
25	118512.5
26	149139.0
27	164682.0
28	179842.5
29	195003.0
30	209676.0
31	224349.0
32	235954.0
33	247559.0
34	260418.5
35	273278.0
36	284429.0
37	295580.0
38	302835.0
39	310090.0
40	314366.0
41	318642.0
42	317741.5
43	316841.0
44	308744.5
45	300648.0
46	284470.0
47	268292.0
48	249647.5
49	231003.0
50	211693.0
51	192383.0
52	178145.0
53	163907.0
54	210137.0
55	256367.0
56	169996.5
57	83626.0
58	70716.5
59	57807.0
60	48680.5
61	39554.0
62	32754.5
63	25955.0
64	20954.5
65	15954.0
66	13016.0
67	10078.0
68	8089.5
69	6101.0
70	4892.0
71	3683.0
72	2907.5
73	2132.0
74	1690.5
75	964.0
76	679.0
77	552.5
78	426.0
79	338.5
80	251.0
81	169.0
82	87.0
83	70.5
84	54.0
85	40.0
86	26.0
87	16.0
88	6.0
89	6.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04642238317522779
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0019417922923490223
20	0.0
21	0.012508754999550679
22	9.031592057437312E-5
23	0.0
24	8.580012454565448E-4
25	0.0
26	0.09067718425667062
27	0.1109079504653302
28	0.12280707300100387
29	0.6914135299571135
30	0.1333966146883491
31	0.03292015304935901
32	0.1199847004830547
33	0.014947284855058755
34	0.012079754376822405
35	0.017318077770136046
36	0.03000746461083547
37	0.03888100380726763
38	0.00819616979212436
39	0.015127916696207498
40	0.025040088979244948
41	0.012486176019407083
42	0.01573754916008452
43	0.01973402864550053
44	0.01533112751749984
45	0.02332408648833186
46	0.01788255227372588
47	0.009076750017724499
48	0.030617097074712493
49	0.01156043783351976
50	0.013773177887591902
51	0.010837910468924775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4428898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.849739944604154
#Duplication Level	Relative count
1	100.0
2	4.401315033723723
3	0.8095881493799539
4	0.5667117045659678
5	0.5136508596403897
6	0.5059925933624713
7	0.44472646313912334
8	0.3993238844914638
9	0.3396988113276699
10++	1.4227964706332838
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG	142386	3.214930666725673	TruSeq Adapter, Index 1 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1586135	3.317036	3.77247	1
AAAAA	1592425	3.2664635	3.4886794	12
GCACA	499680	3.2001812	51.01777	11
CACGT	473760	3.045925	50.863636	14
ACGTC	456850	2.9372065	50.62336	15
GAGCA	416225	2.7540271	53.233295	9
GGAAG	400065	2.7348156	55.708115	5
CTCCA	429940	2.6755388	48.953407	24
GAAGA	567030	2.5950394	37.94139	6
TCCAG	397630	2.556466	50.608356	25
TCCGC	271635	2.5249264	70.41586	35
CGGAA	379895	2.5136428	53.654217	4
CCGCA	260335	2.4105563	70.45051	36
CTGAA	525215	2.335586	36.139244	19
TCGGA	349045	2.3184607	53.557907	3
GTCCG	240190	2.306616	72.71257	34
TCTCG	355890	2.2969675	50.386574	43
CGTCC	246280	2.2892442	70.527885	33
AGAGC	341145	2.2572467	52.91953	8
AGCAC	344090	2.2037113	51.11399	10
CGCAC	236385	2.1887927	70.13543	37
CACAC	347155	2.1520314	49.15009	12
ATCGG	313690	2.0836225	53.25541	2
CTCGT	315250	2.034671	49.9994	44
CCAGT	314655	2.022998	49.968506	26
TCTGA	448290	2.0012255	35.949013	18
CGTCT	308650	1.9920737	50.840576	16
GTCTG	297815	1.9858347	52.619473	17
GTCAC	302910	1.9474865	50.485188	29
AAGAG	425380	1.9467715	37.27315	7
GATCG	291365	1.9353327	52.954742	1
ACTCC	305345	1.9001777	48.344486	23
TCACG	292505	1.8805901	50.151455	30
ACACG	292835	1.8754505	50.641907	13
CAGTC	288200	1.8529122	50.216183	27
TGAAC	410360	1.8248355	35.543682	20
CATCT	396530	1.7133875	33.56382	41
AACTC	390405	1.6804154	34.041065	22
ATCTC	387190	1.6730299	33.75738	42
GAACT	374460	1.6651913	35.34218	21
CACAT	383615	1.6511893	33.316654	39
ACATC	365060	1.5713233	33.282623	40
AGTCA	343745	1.5286044	35.044098	28
TCGTA	314240	1.4028087	34.613483	45
GTATG	281650	1.2989849	35.452656	47
CGTAT	285825	1.2759604	34.401783	46
>>END_MODULE