FastQCFastQC Report
Tue 12 Aug 2014
lane8_Undetermined_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane8_Undetermined_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5024318
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 23892 0.47552722578467366 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5833 0.11609535861384569 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1563310 4.531278 4.8937397 39
TTTTT 2040350 4.5115337 5.648684 18
TTTGA 808575 2.39521 16.375448 8
TGTTT 825630 2.316848 15.537094 6
GTTTG 621200 2.2122595 18.92931 7
TGTGT 613490 2.184802 18.632746 4
CTTTG 570735 2.1216342 20.09556 1
GAGGG 323745 1.9602158 6.315665 11
GTGTT 519855 1.8513426 18.73553 5
TTGAG 462875 1.7401212 11.899039 9
TGAGG 354400 1.6908381 7.444208 10
TTTGT 583220 1.6366074 15.105987 2
TTGTG 443805 1.5805082 18.570934 3
ACTTT 420045 1.2988244 8.250321 3
AACTT 392315 1.2805625 8.846474 2
TAACT 258165 0.8426811 8.4710865 1
Produced by FastQC (version 0.10.1)