##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane8_Undetermined_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5024318	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103371840715496	34.0	31.0	34.0	27.0	34.0
2	31.19732867226955	34.0	31.0	34.0	27.0	34.0
3	31.27238662043286	34.0	31.0	34.0	27.0	34.0
4	34.49984793956115	37.0	35.0	37.0	32.0	37.0
5	34.5219323299202	37.0	35.0	37.0	32.0	37.0
6	34.54653825653551	37.0	36.0	37.0	32.0	37.0
7	34.57567036959046	37.0	37.0	37.0	32.0	37.0
8	34.57389878586507	37.0	36.0	37.0	32.0	37.0
9	36.24751677740143	39.0	38.0	39.0	32.0	39.0
10	36.22559738456045	39.0	38.0	39.0	32.0	39.0
11	36.22727602034744	39.0	38.0	39.0	32.0	39.0
12	36.18711833128397	39.0	38.0	39.0	32.0	39.0
13	36.16450093326099	39.0	38.0	39.0	32.0	39.0
14	37.585171360570726	41.0	39.0	41.0	32.0	41.0
15	37.534726902238276	41.0	39.0	41.0	32.0	41.0
16	37.527819696125924	41.0	39.0	41.0	31.0	41.0
17	37.52389179984229	41.0	39.0	41.0	31.0	41.0
18	37.513176116639116	41.0	39.0	41.0	31.0	41.0
19	37.53335258636098	41.0	39.0	41.0	31.0	41.0
20	37.51451341256664	41.0	39.0	41.0	31.0	41.0
21	37.468780638486656	41.0	39.0	41.0	31.0	41.0
22	37.43084892317724	41.0	39.0	41.0	31.0	41.0
23	37.419578139759466	40.0	39.0	41.0	31.0	41.0
24	37.409670725459655	41.0	39.0	41.0	31.0	41.0
25	37.35092703129062	40.0	38.0	41.0	31.0	41.0
26	37.29425864366069	40.0	38.0	41.0	30.0	41.0
27	37.25784932402766	40.0	38.0	41.0	30.0	41.0
28	37.2185705602233	40.0	38.0	41.0	30.0	41.0
29	37.179173571418055	40.0	38.0	41.0	30.0	41.0
30	37.140060402227725	40.0	38.0	41.0	30.0	41.0
31	37.09885918845105	40.0	38.0	41.0	30.0	41.0
32	37.05747665653328	40.0	38.0	41.0	30.0	41.0
33	36.95123756099833	40.0	38.0	41.0	30.0	41.0
34	36.85944858585782	40.0	38.0	41.0	29.0	41.0
35	36.79742484452616	40.0	38.0	41.0	29.0	41.0
36	36.757141964342225	40.0	38.0	41.0	29.0	41.0
37	36.710632567444975	40.0	38.0	41.0	28.0	41.0
38	36.66155884241404	40.0	38.0	41.0	28.0	41.0
39	36.6059409456169	40.0	38.0	41.0	28.0	41.0
40	36.54413952301586	40.0	38.0	41.0	28.0	41.0
41	36.4806584694679	40.0	37.0	41.0	27.0	41.0
42	36.401543254228734	40.0	37.0	41.0	27.0	41.0
43	36.351325891394616	40.0	37.0	41.0	27.0	41.0
44	36.268281784711874	40.0	37.0	41.0	27.0	41.0
45	36.21815597659224	40.0	37.0	41.0	27.0	41.0
46	36.14126215737141	40.0	36.0	41.0	26.0	41.0
47	36.06013433066936	40.0	36.0	41.0	26.0	41.0
48	36.24910963040158	40.0	37.0	41.0	27.0	41.0
49	36.26680496736074	40.0	37.0	41.0	27.0	41.0
50	36.22439702264069	40.0	36.0	41.0	27.0	41.0
51	34.82483951055646	38.0	35.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24644.0
3	581.0
4	551.0
5	692.0
6	2624.0
7	11005.0
8	38784.0
9	53763.0
10	52787.0
11	33307.0
12	21376.0
13	17672.0
14	16220.0
15	15260.0
16	13955.0
17	13811.0
18	13312.0
19	12428.0
20	12613.0
21	12731.0
22	12933.0
23	13116.0
24	14034.0
25	15248.0
26	16669.0
27	18565.0
28	21572.0
29	24613.0
30	28621.0
31	34842.0
32	44715.0
33	58856.0
34	84085.0
35	129961.0
36	211979.0
37	379863.0
38	963681.0
39	2581973.0
40	876.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.421115414706705	27.411274561886458	30.76991030061849	22.397699722788346
2	22.05927060036125	29.355508523338486	29.62183095420363	18.963389922096635
3	22.381746396849145	27.994651726933125	28.959333875070758	20.664268001146972
4	21.92232881482104	26.019566879184826	30.119246300943324	21.938858005050808
5	24.97287577977755	26.407572405806757	28.659988467828303	19.95956334658739
6	21.78908905479248	26.00971752805427	31.03458972482881	21.166603692324443
7	23.97109418025333	26.307870073899515	29.50759407138923	20.213441674457922
8	22.546094044579792	26.459114376314414	30.432776866477575	20.56201471262822
9	22.489066717037673	26.39933615923214	30.37543741135717	20.736159712373016
10	22.498619381975523	27.483295973079	29.39312255064151	20.624962094303967
11	24.16571669659915	26.4938825798951	28.585545463701866	20.754855259803882
12	22.06235122451015	28.33300155289268	28.621630509274297	20.983016713322876
13	23.029939598904015	26.598167864821153	29.154567039965496	21.217325496309332
14	23.457972165849938	26.941902873826766	28.411730630297704	21.188394330025588
15	22.820918857880432	27.634067083661023	28.104515222488075	21.44049883597047
16	23.140048414808064	26.931097931702713	28.495234718775414	21.433618934713802
17	23.116988945957928	27.039774991041487	28.437082387044587	21.406153675956
18	22.822093999034653	26.91862456264032	28.526085362996017	21.73319607532901
19	22.61327083640841	27.016688433375748	28.76355946477328	21.606481265442564
20	22.62720403426175	27.083710450730713	28.6738509866993	21.615234528308232
21	22.548499190736663	27.02979616022232	28.66024586295806	21.761458786082954
22	22.413224147879117	27.090501418814807	28.824474745818236	21.671799687487848
23	22.574189955273223	27.174649835102294	28.61106362124736	21.640096588377126
24	22.356071070756542	27.202968900039586	28.564695418762927	21.87626461044094
25	22.454007808692833	27.262945603338995	28.523240505375814	21.75980608259236
26	22.42533075944063	27.319253875718495	28.517785289974746	21.73763007486613
27	22.478125654748098	27.373948847623318	28.29381108561892	21.854114412009668
28	22.43484604341722	27.360267552475804	28.316063778738297	21.88882262536868
29	22.52583187557062	27.329012390532682	28.218342728471402	21.9268130054253
30	22.39661514392102	27.315466769500034	28.278629063647358	22.00928902293159
31	22.32020502536298	27.279521180516987	28.43634136602852	21.963932428091518
32	22.62175743321827	27.312745264630117	28.20659079987567	21.858906502275943
33	22.54038035012596	27.187806488419948	28.172655944513174	22.099157216940917
34	22.42755437512825	27.249726633542775	28.29984334887262	22.022875642456352
35	22.63811379752905	27.286847337139747	28.12232891871918	21.95270994661202
36	22.541914400651493	27.188747081730824	28.12649027082904	22.14284824678865
37	22.559574533908197	27.136802358914895	28.178209125889286	22.125413981287622
38	22.67828801716483	27.224074959518806	28.2391785632491	21.85845846006726
39	22.500388133030913	27.123981439508587	28.146101909172653	22.229528518287843
40	22.43242646143217	27.27884202112062	28.162509891504754	22.126221625942456
41	22.54793970705315	27.300415869538185	28.114014267051285	22.037630156357384
42	22.425707300091062	27.18432520506816	28.129789217672474	22.260178277168304
43	22.433020928927398	27.304393974794078	28.16643663875019	22.096148457528336
44	22.556423879451344	27.2447506461577	28.125283534560907	22.07354193983005
45	22.603436346655155	27.112068160892754	28.076544713072742	22.207950779379352
46	22.45332615302445	27.20627463758535	28.188617939683397	22.15178126970681
47	22.611492868453862	27.2657471479128	27.978651169793284	22.144108813840052
48	22.521684812674962	27.10594059624481	27.94977563214387	22.42259895893636
49	22.38973926755116	27.30789284486011	28.112152677463076	22.190215210125654
50	22.43737093854155	27.200684821228172	28.223500016261294	22.138444223968985
51	22.559218850448985	26.872153297025637	28.18179226391464	22.386835588610737
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.81881513749505
2	41.02266052245788
3	43.04601439799612
4	43.86118681987185
5	44.93243912636494
6	42.95569274711693
7	44.18453585471126
8	43.108108757208015
9	43.22522642941069
10	43.12358147627949
11	44.92057195640303
12	43.04536793783302
13	44.24726509521335
14	44.646366495875526
15	44.2614176938509
16	44.57366734952187
17	44.52314262191393
18	44.55529007436366
19	44.21975210185097
20	44.24243856256999
21	44.30995797681962
22	44.08502383536696
23	44.21428654365035
24	44.23233568119749
25	44.21381389128519
26	44.16296083430676
27	44.33224006675777
28	44.3236686687859
29	44.45264488099592
30	44.40590416685261
31	44.2841374534545
32	44.48066393549421
33	44.63953756706688
34	44.4504300175846
35	44.590823744141076
36	44.68476264744014
37	44.684988515195826
38	44.53674647723209
39	44.72991665131876
40	44.55864808737463
41	44.585569863410534
42	44.68588557725936
43	44.52916938645573
44	44.629965819281395
45	44.811387126034504
46	44.60510742273126
47	44.755601682293914
48	44.94428377161132
49	44.579954477676814
50	44.57581516251054
51	44.94605443905972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27796.0
1	14817.5
2	1839.0
3	2154.5
4	2470.0
5	6817.0
6	11164.0
7	12845.0
8	14526.0
9	9777.0
10	5028.0
11	5242.5
12	5457.0
13	6174.0
14	6891.0
15	8502.0
16	10113.0
17	13441.0
18	16769.0
19	20308.0
20	23847.0
21	27569.5
22	31292.0
23	36926.5
24	42561.0
25	52087.0
26	73144.0
27	84675.0
28	91843.0
29	99011.0
30	114388.0
31	129765.0
32	142594.0
33	155423.0
34	186246.0
35	217069.0
36	247810.5
37	278552.0
38	317062.5
39	355573.0
40	379620.0
41	403667.0
42	437907.0
43	472147.0
44	487650.0
45	503153.0
46	486889.0
47	470625.0
48	416223.5
49	361822.0
50	329681.0
51	297540.0
52	262613.5
53	227687.0
54	199511.5
55	171336.0
56	154453.0
57	137570.0
58	119751.5
59	101933.0
60	96592.5
61	91252.0
62	74385.0
63	57518.0
64	49187.0
65	40856.0
66	33917.0
67	26978.0
68	23439.5
69	19901.0
70	17549.5
71	15198.0
72	12776.5
73	10355.0
74	8732.5
75	6130.5
76	5151.0
77	4739.0
78	4327.0
79	4333.0
80	4339.0
81	3801.5
82	3264.0
83	2936.5
84	2609.0
85	2360.0
86	2111.0
87	2264.5
88	2418.0
89	1725.5
90	1033.0
91	709.0
92	385.0
93	302.5
94	220.0
95	179.5
96	139.0
97	124.5
98	110.0
99	120.0
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.091292390330389
2	0.5521744443723506
3	0.5337440822814161
4	0.5393368811448638
5	0.5545230218310226
6	0.5488108037747611
7	0.5426010057484418
8	0.5531895075112682
9	0.5320921167808248
10	0.564594040424989
11	0.6030868269086471
12	0.6181137420043874
13	0.6934075430735076
14	0.7391052875236002
15	0.8199520810585635
16	0.7446582799894433
17	0.6896458384998719
18	0.6227312841265222
19	0.6213579634091632
20	0.6029475045170309
21	0.6762509857059207
22	0.7235210828613953
23	0.5791830851470786
24	0.5517166707998976
25	0.6304736284606189
26	0.6809083342256601
27	0.6357081697456252
28	0.5794020203339041
29	0.570206742487239
30	0.5421034257783843
31	0.5545628282286272
32	0.5558764393495794
33	0.6425946765312228
34	0.8722377843122192
35	0.9201447838293676
36	0.9194481718712868
37	0.92422493958384
38	0.929439577670044
39	0.9027095816785482
40	0.9015750993468168
41	0.9265934202413143
42	0.9222943293000165
43	0.8421839541207383
44	0.84711994742371
45	0.8427213404884006
46	0.8024372661125351
47	0.7190826695284812
48	0.8112742863807585
49	0.8650925359421916
50	0.8591016731026977
51	0.8669037270332013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5024318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.123364113465755
#Duplication Level	Relative count
1	100.0
2	4.3497826885043995
3	1.3139351736715577
4	0.6631849457017326
5	0.4322544735377364
6	0.35054061415663007
7	0.2842221195864568
8	0.24277306048009853
9	0.2190878838478938
10++	10.570102201537168
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	23892	0.47552722578467366	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5833	0.11609535861384569	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1563310	4.531278	4.8937397	39
TTTTT	2040350	4.5115337	5.648684	18
TTTGA	808575	2.39521	16.375448	8
TGTTT	825630	2.316848	15.537094	6
GTTTG	621200	2.2122595	18.92931	7
TGTGT	613490	2.184802	18.632746	4
CTTTG	570735	2.1216342	20.09556	1
GAGGG	323745	1.9602158	6.315665	11
GTGTT	519855	1.8513426	18.73553	5
TTGAG	462875	1.7401212	11.899039	9
TGAGG	354400	1.6908381	7.444208	10
TTTGT	583220	1.6366074	15.105987	2
TTGTG	443805	1.5805082	18.570934	3
ACTTT	420045	1.2988244	8.250321	3
AACTT	392315	1.2805625	8.846474	2
TAACT	258165	0.8426811	8.4710865	1
>>END_MODULE