FastQCFastQC Report
Tue 12 Aug 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane8_Undetermined_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5024318
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7211 0.14352196656342214 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1569705 4.571574 5.6658783 18
TGTTT 621395 2.1650083 20.450907 6
TGTGT 500380 2.0856233 23.676802 4
GTTTG 481145 2.0054502 23.83416 7
TTTGA 547305 1.8211923 19.250235 8
CTTTG 411280 1.7702501 24.350037 1
TTTGT 499720 1.7410793 20.102627 2
GTGTT 402000 1.6755675 23.578068 5
GAGGG 284415 1.6203588 5.906 11
TTGAG 375810 1.4960248 13.201756 9
TTGTG 354245 1.476521 23.405048 3
TGAGG 301230 1.4345405 7.5589194 10
TTGAT 312960 1.0413944 5.2023077 9
Produced by FastQC (version 0.10.1)