##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane8_Undetermined_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5024318	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.936062964167476	34.0	33.0	34.0	31.0	34.0
2	33.14009324250575	34.0	34.0	34.0	31.0	34.0
3	33.182344150987255	34.0	34.0	34.0	31.0	34.0
4	36.52356638254187	37.0	37.0	37.0	35.0	37.0
5	36.455126247980324	37.0	37.0	37.0	35.0	37.0
6	36.408968341573924	37.0	37.0	37.0	35.0	37.0
7	36.419828920064376	37.0	37.0	37.0	35.0	37.0
8	36.399837749123364	37.0	37.0	37.0	35.0	37.0
9	38.1815408578836	39.0	39.0	39.0	37.0	39.0
10	38.18577605955674	39.0	39.0	39.0	37.0	39.0
11	38.17377602293485	39.0	39.0	39.0	37.0	39.0
12	38.133000936644535	39.0	38.0	39.0	37.0	39.0
13	38.10401033533307	39.0	38.0	39.0	37.0	39.0
14	39.61336424167419	41.0	40.0	41.0	37.0	41.0
15	39.59551863556407	41.0	40.0	41.0	37.0	41.0
16	39.56467325515622	41.0	40.0	41.0	37.0	41.0
17	39.57140292473526	41.0	40.0	41.0	37.0	41.0
18	39.558946706796824	41.0	40.0	41.0	37.0	41.0
19	39.563290579935426	41.0	40.0	41.0	37.0	41.0
20	39.52702953913347	41.0	40.0	41.0	37.0	41.0
21	39.48874533817326	41.0	39.0	41.0	37.0	41.0
22	39.44129750545248	41.0	39.0	41.0	37.0	41.0
23	39.377193282750014	41.0	39.0	41.0	37.0	41.0
24	39.34807749031809	41.0	39.0	41.0	36.0	41.0
25	39.29906287778759	41.0	39.0	41.0	36.0	41.0
26	39.190986717003184	40.0	39.0	41.0	36.0	41.0
27	39.0808312690399	40.0	39.0	41.0	36.0	41.0
28	39.0424953197628	40.0	39.0	41.0	36.0	41.0
29	38.98810365904387	40.0	39.0	41.0	36.0	41.0
30	38.91536363741308	40.0	39.0	41.0	35.0	41.0
31	38.790791904493304	40.0	38.0	41.0	35.0	41.0
32	38.711379534496025	40.0	38.0	41.0	35.0	41.0
33	38.602316573115	40.0	38.0	41.0	35.0	41.0
34	38.52096662671431	40.0	38.0	41.0	35.0	41.0
35	38.40669957594245	40.0	38.0	41.0	35.0	41.0
36	38.317024519546734	40.0	38.0	41.0	34.0	41.0
37	38.517523572353504	40.0	38.0	41.0	35.0	41.0
38	38.57332836018739	40.0	38.0	41.0	35.0	41.0
39	38.5503622581214	40.0	38.0	41.0	35.0	41.0
40	38.529677261670145	40.0	38.0	41.0	35.0	41.0
41	38.41598461721571	40.0	38.0	41.0	35.0	41.0
42	38.352252186266874	40.0	38.0	41.0	35.0	41.0
43	38.308276466577155	40.0	38.0	41.0	34.0	41.0
44	38.26927973109982	40.0	38.0	41.0	34.0	41.0
45	38.167347687785686	40.0	38.0	41.0	34.0	41.0
46	38.10139226856262	40.0	38.0	41.0	34.0	41.0
47	38.02845082655994	40.0	38.0	41.0	34.0	41.0
48	37.95970378467287	40.0	38.0	41.0	34.0	41.0
49	37.897569182523874	40.0	37.0	41.0	33.0	41.0
50	37.99882491514271	40.0	37.0	41.0	34.0	41.0
51	37.70919416326753	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	13.0
12	15.0
13	36.0
14	44.0
15	102.0
16	196.0
17	326.0
18	683.0
19	1321.0
20	2223.0
21	3772.0
22	5859.0
23	9045.0
24	12317.0
25	16206.0
26	18769.0
27	21988.0
28	25341.0
29	28799.0
30	33311.0
31	39247.0
32	47735.0
33	62484.0
34	87698.0
35	134046.0
36	224547.0
37	424585.0
38	1096487.0
39	2725754.0
40	1364.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.41131953794742	29.179300376477237	27.106281191861324	23.30309889371402
2	22.6044969990146	28.848616958802392	28.877641414593846	19.66924462758916
3	21.230344098442814	27.497204595728213	29.737468846518073	21.534982459310896
4	21.93495714244202	27.524253042900547	29.089380887117418	21.451408927540015
5	24.520571556406882	27.932259833975408	27.15675128714038	20.390417322477326
6	22.348187355975476	27.32319092859966	28.702323380008988	21.62629833541587
7	24.52744078716778	27.953834680355893	26.603693934129215	20.91503059834711
8	22.229882742294578	27.862348680955307	28.659611115379242	21.248157461370877
9	21.739010150233327	27.81452129423337	29.07105004102049	21.375418514512816
10	22.0565457839253	28.07360123304297	28.48330858038842	21.386544402643302
11	24.147496237300267	27.828572952587795	26.668654332787057	21.35527647732488
12	22.06032739169774	29.90533242521672	26.57970693733955	21.454633245745992
13	23.086475032616704	28.34450885603515	26.964941198347592	21.604074913000552
14	22.76309931344827	28.383859410778655	27.190098037555664	21.662943238217416
15	22.785310485950404	28.083010415312813	27.23786716271545	21.89381193602133
16	22.778895013825423	28.292811756160297	27.08432462168055	21.843968608333732
17	22.87058661493958	28.41973776341386	26.934083392014596	21.77559222963196
18	22.662518574660282	28.054613581385574	27.16273133985548	22.120136504098667
19	22.46864133765106	28.414692851742	27.24342396815936	21.873241842447584
20	22.495548610605233	28.40939805432586	27.202875480119115	21.89217785494979
21	22.456759305442052	28.226298574254255	27.153615674804023	22.163326445499667
22	22.473597411628802	28.51202889626015	27.08699966841271	21.92737402369834
23	22.664856547383256	28.409769070449336	27.093464659876066	21.83190972229134
24	22.410105828330448	28.267714168966666	27.15244745056445	22.16973255213843
25	22.464422036980938	28.61170411586209	26.912269486127272	22.011604361029697
26	22.54609022778025	28.424332039251084	26.965521856658814	22.064055876309848
27	22.594215303429028	28.22434506899806	26.92712780956955	22.254311818003366
28	22.527154153903297	28.57182098790621	26.73761803027459	22.163406827915903
29	22.722944976764694	28.596117728205144	26.691847786599464	21.9890895084307
30	22.59755249335211	28.325488046139284	26.821685241742742	22.255274218765862
31	22.53760853157777	28.50509600536812	26.728919945090805	22.228375517963304
32	22.78238087858159	28.596012865220754	26.679815284561137	21.941790971636514
33	22.67928449758055	28.31476362011672	26.72710300506229	22.278848877240435
34	22.613319994471393	28.547976723207245	26.62511036771421	22.21359291460715
35	22.789305687922827	28.41965832231836	26.75513672121186	22.03589926854696
36	22.84970107262177	28.229234937736784	26.60613520469332	22.314928784948126
37	22.813811479694234	28.51818704877517	26.539541000561	22.128460470969593
38	22.843006003121	28.438674089113224	26.718055902843	22.000264004922773
39	22.691426940163314	28.204296993213916	26.743565073220125	22.360710993402638
40	22.681231712814512	28.60846201705987	26.482714337472284	22.227591932653333
41	22.69235579433834	28.62752243283535	26.66084094213713	22.01928083068918
42	22.659913727221102	28.336565203631775	26.702538498100186	22.300982571046937
43	22.735605263189385	28.584021133167592	26.438496475478722	22.2418771281643
44	22.762868625792247	28.5285205131336	26.573903233009368	22.13470762806479
45	22.73718428030953	28.328927029858857	26.618309048789072	22.315579641042543
46	22.73569589596189	28.52862734093519	26.432363549830935	22.303313213271984
47	22.727450303484993	28.496176639189706	26.511365638626028	22.265007418699266
48	22.62124848911385	28.359925859476533	26.567226812057122	22.451598839352503
49	22.588571583331806	28.66443842067489	26.41291957842818	22.334070417565123
50	22.674552211432115	28.46757597759155	26.60548924084738	22.252382570128947
51	22.715778245909192	28.26526641470416	26.61171765063881	22.407237688747838
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.71441843166144
2	42.27374162660376
3	42.76532655775371
4	43.38636606998204
5	44.91098887888421
6	43.97448569139135
7	45.44247138551489
8	43.47804020366545
9	43.11442866474614
10	43.443090186568604
11	45.50277271462515
12	43.51496063744373
13	44.690549945617256
14	44.42604255166568
15	44.679122421971734
16	44.62286362215915
17	44.646178844571544
18	44.78265507875895
19	44.34188318009864
20	44.38772646555502
21	44.62008575094172
22	44.40097143532714
23	44.496766269674595
24	44.57983838046888
25	44.47602639801064
26	44.610146104090106
27	44.84852712143239
28	44.6905609818192
29	44.71203448519539
30	44.852826712117974
31	44.765984049541075
32	44.724171850218106
33	44.95813337482099
34	44.826912909078544
35	44.82520495646978
36	45.1646298575699
37	44.94227195066382
38	44.843270008043774
39	45.05213793356596
40	44.90882364546785
41	44.711636625027516
42	44.96089629826804
43	44.97748239135368
44	44.89757625385703
45	45.052763921352074
46	45.039009109233874
47	44.99245772218426
48	45.072847328466345
49	44.92264200089693
50	44.92693478156106
51	45.12301593465703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5370.0
1	3154.0
2	938.0
3	1178.5
4	1419.0
5	3537.0
6	5655.0
7	8654.0
8	11653.0
9	7138.5
10	2624.0
11	3049.5
12	3475.0
13	3972.5
14	4470.0
15	6357.5
16	8245.0
17	11612.5
18	14980.0
19	18474.0
20	21968.0
21	25518.0
22	29068.0
23	34621.5
24	40175.0
25	49916.5
26	73869.0
27	88080.0
28	91479.0
29	94878.0
30	105693.5
31	116509.0
32	134252.5
33	151996.0
34	177613.0
35	203230.0
36	241377.0
37	279524.0
38	304508.5
39	329493.0
40	362617.0
41	395741.0
42	435216.0
43	474691.0
44	489043.5
45	503396.0
46	481166.5
47	458937.0
48	431695.0
49	404453.0
50	370865.0
51	337277.0
52	298049.0
53	258821.0
54	226464.5
55	194108.0
56	169512.5
57	144917.0
58	122296.0
59	99675.0
60	90270.5
61	80866.0
62	66824.0
63	52782.0
64	45446.5
65	38111.0
66	31897.0
67	25683.0
68	23330.5
69	20978.0
70	18483.5
71	15989.0
72	13459.0
73	10929.0
74	9105.0
75	6202.5
76	5124.0
77	4710.0
78	4296.0
79	4436.5
80	4577.0
81	3982.5
82	3388.0
83	3058.5
84	2729.0
85	2385.5
86	2042.0
87	2384.5
88	2727.0
89	1779.0
90	831.0
91	519.5
92	208.0
93	138.0
94	68.0
95	47.0
96	26.0
97	24.5
98	23.0
99	129.5
100	236.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2506608857162306
2	0.019266296440631346
3	0.0
4	0.0
5	3.980639760461022E-5
6	0.0
7	5.373863676622379E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0315465701016536
14	0.08564346444631889
15	0.0016121591029867137
16	0.06956167981405635
17	0.0
18	0.0
19	0.025953771238205862
20	8.757407473014248E-4
21	0.0
22	0.0
23	0.014091464752032018
24	9.752567413129503E-4
25	0.0
26	0.06993984059130015
27	0.12783824590720572
28	0.00997150259995486
29	0.014190980746043542
30	0.018310942898120703
31	0.15994210557532385
32	0.08502646528344743
33	0.21451667669124447
34	0.2069335579475662
35	0.3633328941360798
36	0.3932474019359444
37	0.23585290580731555
38	0.1843036209093453
39	0.23002126855824015
40	0.12857466426289102
41	0.3768073597252403
42	0.2542036551030408
43	0.1994300519990972
44	0.07859773207030288
45	0.2348975522648049
46	0.1347844622892102
47	0.14461664249754894
48	0.11424436112523133
49	0.05433573273029295
50	0.05254444483808549
51	0.0584357916835678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5024318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	56.91253807486273
#Duplication Level	Relative count
1	100.0
2	4.825811085837423
3	1.3977014246378547
4	0.7003471806536574
5	0.4998204237998324
6	0.35256793966239675
7	0.29630073027654735
8	0.26696995091583864
9	0.21429426553334133
10++	11.163653777086077
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7211	0.14352196656342214	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1569705	4.571574	5.6658783	18
TGTTT	621395	2.1650083	20.450907	6
TGTGT	500380	2.0856233	23.676802	4
GTTTG	481145	2.0054502	23.83416	7
TTTGA	547305	1.8211923	19.250235	8
CTTTG	411280	1.7702501	24.350037	1
TTTGT	499720	1.7410793	20.102627	2
GTGTT	402000	1.6755675	23.578068	5
GAGGG	284415	1.6203588	5.906	11
TTGAG	375810	1.4960248	13.201756	9
TTGTG	354245	1.476521	23.405048	3
TGAGG	301230	1.4345405	7.5589194	10
TTGAT	312960	1.0413944	5.2023077	9
>>END_MODULE