FastQCFastQC Report
Tue 12 Aug 2014
lane7_Undetermined_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane7_Undetermined_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4649213
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 15497 0.33332523160371447 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1439085 4.3354454 4.649254 43
TTTTT 1389300 3.424096 4.221155 18
TGTGT 723395 2.8020368 26.123274 4
TTTGA 822860 2.6466074 22.395777 8
TGTTT 855375 2.6428998 21.538286 6
GTTTG 664715 2.5747428 26.4239 7
CTTTG 587675 2.40838 28.033657 1
GTGTT 578210 2.2396696 25.99889 5
GAGGG 320945 2.0338407 7.872361 11
TTGTG 503945 1.9520078 25.886024 3
TTGAG 478350 1.9287841 16.25993 9
TTTGT 619605 1.914428 21.089457 2
TGAGG 355610 1.7975708 9.843034 10
TTGAC 369315 1.5755213 5.474505 9
ACTTT 417150 1.4195325 10.80082 3
TGAGT 330340 1.3319839 5.033013 10
AACTT 375485 1.3301027 11.519344 2
TTGAT 374330 1.2039772 5.4449053 9
TAACT 262890 0.9312507 11.175912 1
Produced by FastQC (version 0.10.1)