##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane7_Undetermined_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4649213	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99959003814194	34.0	31.0	34.0	27.0	34.0
2	31.088274295025847	34.0	31.0	34.0	27.0	34.0
3	31.179494895157525	34.0	31.0	34.0	27.0	34.0
4	34.41060261166783	37.0	35.0	37.0	31.0	37.0
5	34.437345202295525	37.0	35.0	37.0	31.0	37.0
6	34.461590380995666	37.0	35.0	37.0	31.0	37.0
7	34.49057012444902	37.0	36.0	37.0	31.0	37.0
8	34.49477750320323	37.0	36.0	37.0	31.0	37.0
9	36.15955926304086	39.0	38.0	39.0	31.0	39.0
10	36.14282761404995	39.0	38.0	39.0	31.0	39.0
11	36.13631747996919	39.0	38.0	39.0	31.0	39.0
12	36.10764424000363	39.0	38.0	39.0	31.0	39.0
13	36.070523333734116	39.0	38.0	39.0	31.0	39.0
14	37.45997720474411	41.0	39.0	41.0	31.0	41.0
15	37.41375088644035	41.0	39.0	41.0	31.0	41.0
16	37.39836849806623	41.0	38.0	41.0	31.0	41.0
17	37.38885247890342	41.0	38.0	41.0	31.0	41.0
18	37.375930506948166	41.0	38.0	41.0	31.0	41.0
19	37.38491310249713	41.0	39.0	41.0	30.0	41.0
20	37.36400934953937	41.0	39.0	41.0	30.0	41.0
21	37.337781469681	40.0	38.0	41.0	30.0	41.0
22	37.28468818270963	40.0	38.0	41.0	30.0	41.0
23	37.27966647258364	40.0	38.0	41.0	30.0	41.0
24	37.26093470013097	40.0	38.0	41.0	30.0	41.0
25	37.22446293598508	40.0	38.0	41.0	30.0	41.0
26	37.1582461375721	40.0	38.0	41.0	30.0	41.0
27	37.10668192659704	40.0	38.0	41.0	30.0	41.0
28	37.089645494839665	40.0	38.0	41.0	30.0	41.0
29	37.052452748454414	40.0	38.0	41.0	29.0	41.0
30	37.01760233398642	40.0	38.0	41.0	29.0	41.0
31	36.96590111057506	40.0	38.0	41.0	29.0	41.0
32	36.92333304582948	40.0	38.0	41.0	29.0	41.0
33	36.80949765046256	40.0	38.0	41.0	28.0	41.0
34	36.672071165592975	40.0	38.0	41.0	27.0	41.0
35	36.63026753130046	40.0	38.0	41.0	27.0	41.0
36	36.588692107675	40.0	38.0	41.0	27.0	41.0
37	36.53403834154297	40.0	38.0	41.0	27.0	41.0
38	36.48179294000942	40.0	38.0	41.0	27.0	41.0
39	36.43012053868042	40.0	37.0	41.0	27.0	41.0
40	36.37017060736946	40.0	37.0	41.0	27.0	41.0
41	36.279976632604274	40.0	37.0	41.0	26.0	41.0
42	36.21541258703355	40.0	37.0	41.0	26.0	41.0
43	36.16388451120652	40.0	37.0	41.0	26.0	41.0
44	36.09715063603238	40.0	37.0	41.0	26.0	41.0
45	36.02390963803981	40.0	36.0	41.0	25.0	41.0
46	35.96101318653286	40.0	36.0	41.0	25.0	41.0
47	35.89039177168265	40.0	36.0	41.0	25.0	41.0
48	36.07307537856407	40.0	36.0	41.0	26.0	41.0
49	36.107496688149155	40.0	36.0	41.0	26.0	41.0
50	36.0727576903876	40.0	36.0	41.0	26.0	41.0
51	34.668839005655364	38.0	34.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16548.0
3	661.0
4	522.0
5	602.0
6	2095.0
7	9749.0
8	34128.0
9	51101.0
10	52685.0
11	35329.0
12	22384.0
13	17943.0
14	15978.0
15	15077.0
16	14189.0
17	13554.0
18	13194.0
19	12431.0
20	12329.0
21	12565.0
22	13092.0
23	13593.0
24	14876.0
25	16371.0
26	18489.0
27	19038.0
28	21614.0
29	24361.0
30	28969.0
31	35324.0
32	44928.0
33	59592.0
34	84894.0
35	128320.0
36	203060.0
37	360867.0
38	883475.0
39	2324497.0
40	789.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.280590556745413	27.035279025016035	30.840695360998467	22.843435057240082
2	21.787366236164356	29.207576921364563	30.34572308006185	18.659333762409233
3	22.202153584070548	28.089224603425322	29.61180257761118	20.096819234892955
4	21.66776841894622	25.81220825084598	30.860250629014473	21.65977270119333
5	25.585574176907066	26.11586921794934	28.737846369816122	19.560710235327473
6	21.647375684939643	25.696192415933584	32.110748017805726	20.545683881321043
7	24.55500620449479	26.089047565777797	29.64187129117847	19.71407493854894
8	22.734294308148677	26.278747492916665	30.918455373160697	20.06850282577396
9	22.504673074735305	26.26003558854837	31.007024669128608	20.228266667587718
10	22.594025814254902	27.370144443842303	29.860824284857134	20.175005457045657
11	24.90077513350962	26.333497519918424	28.41063064931474	20.35509669725722
12	21.913886740102942	29.00072560679111	28.559485823665238	20.525901829440713
13	23.415345490290413	26.61323120427945	29.06018368692576	20.911239618504375
14	24.013506397488143	27.002600897035688	28.016100152175312	20.967792553300857
15	23.207720508613473	27.999924633171453	27.578021993253373	21.2143328649617
16	23.48421833879096	27.089952449503162	28.02578968910256	21.400039522603315
17	23.429169607242898	27.141058711323176	28.096846079148737	21.3329256022852
18	23.14261110069197	27.056625266255974	28.143042647895772	21.65772098515629
19	22.93224322067619	27.229981935074022	28.38458797190535	21.453186872344435
20	22.795070241529086	27.326823670525556	28.3827399605197	21.495366127425655
21	22.713897292271238	27.28485905280213	28.32704188132331	21.674201773603325
22	22.576806729532407	27.45811748005963	28.465645731850074	21.499430058557884
23	22.693206770139227	27.48786444500754	28.307344118763687	21.511584666089544
24	22.4862324160132	27.54301478312975	28.277934425747564	21.69281837510949
25	22.556826915032502	27.60071486677748	28.19179649947025	21.650661718719768
26	22.50735622205036	27.629511271606127	28.167308555376046	21.695823950967462
27	22.5689279660247	27.702734274611196	28.01255642864745	21.715781330716656
28	22.53936146417839	27.764195357773048	27.978639834633768	21.717803343414797
29	22.650797682547747	27.64748012489222	27.886923120785017	21.814799071775013
30	22.525883378563904	27.647198080530288	27.939879195059973	21.887039345845828
31	22.487160239135484	27.616735163393045	28.01192437097323	21.88418022649824
32	22.72250849715955	27.62956143121243	27.858305355100693	21.789624716527324
33	22.603661884207764	27.601340813253078	27.785793488281946	22.009203814257216
34	22.568702113675847	27.570306687285633	27.91198647948739	21.949004719551127
35	22.718958555407163	27.645139423837655	27.743942607331796	21.891959413423383
36	22.665539098099984	27.54380705901046	27.782619482726474	22.008034360163084
37	22.64000309099284	27.483745154586543	27.809102539780252	22.067149214640366
38	22.749381481743562	27.600218624877304	27.84280715037059	21.80759274300854
39	22.573078812134128	27.51684331465325	27.78339896527726	22.126678907935364
40	22.518463413448515	27.648961639155285	27.806187109811738	22.02638783758446
41	22.62634600379241	27.640737969823114	27.78450565583206	21.948410370552416
42	22.512335999492795	27.51691083904772	27.82755264973752	22.143200511721968
43	22.572036498434677	27.6583787480721	27.79750885077494	21.972075902718288
44	22.654692415601467	27.527509201187073	27.814298602408723	22.003499780802745
45	22.66634623163852	27.467774481124085	27.76154515043058	22.104334136806813
46	22.549522235668153	27.517707425190736	27.787341739209637	22.145428599931467
47	22.646501458989647	27.586087324388096	27.692455731844117	22.074955484778137
48	22.59020279739091	27.416066558431694	27.634821520767836	22.358909123409557
49	22.479635101254285	27.577385747996463	27.7848072174953	22.158171933253954
50	22.54082085669416	27.521608426445898	27.81693569707928	22.12063501978066
51	22.649491074829484	27.171923087268233	27.81919642663469	22.35938941126759
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.1240256139855
2	40.446699998573585
3	42.298972818963506
4	43.32754112013955
5	45.146284412234536
6	42.193059566260686
7	44.269081143043735
8	42.80279713392264
9	42.732939742323026
10	42.76903127130056
11	45.255871830766836
12	42.43978856954365
13	44.32658510879479
14	44.981298950789004
15	44.422053373575174
16	44.884257861394275
17	44.7620952095281
18	44.80033208584826
19	44.38543009302063
20	44.29043636895474
21	44.38809906587456
22	44.0762367880903
23	44.204791436228774
24	44.179050791122684
25	44.20748863375227
26	44.20318017301783
27	44.284709296741354
28	44.25716480759319
29	44.46559675432276
30	44.41292272440974
31	44.37134046563372
32	44.51213321368688
33	44.61286569846498
34	44.51770683322698
35	44.61091796883055
36	44.673573458263064
37	44.7071523056332
38	44.5569742247521
39	44.699757720069485
40	44.54485125103298
41	44.57475637434483
42	44.65553651121476
43	44.544112401152965
44	44.658192196404215
45	44.77068036844533
46	44.69495083559962
47	44.721456943767784
48	44.94911192080047
49	44.637807034508235
50	44.66145587647482
51	45.00888048609707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17941.0
1	9760.0
2	1579.0
3	1832.0
4	2085.0
5	3657.5
6	5230.0
7	6464.0
8	7698.0
9	6314.5
10	4931.0
11	5094.0
12	5257.0
13	5935.5
14	6614.0
15	8228.0
16	9842.0
17	12590.0
18	15338.0
19	18948.0
20	22558.0
21	26854.0
22	31150.0
23	37031.5
24	42913.0
25	51712.5
26	72039.0
27	83566.0
28	91642.5
29	99719.0
30	114896.0
31	130073.0
32	143277.0
33	156481.0
34	183772.5
35	211064.0
36	237184.0
37	263304.0
38	296232.0
39	329160.0
40	349513.5
41	369867.0
42	398169.0
43	426471.0
44	438021.5
45	449572.0
46	435412.0
47	421252.0
48	377591.5
49	333931.0
50	304335.5
51	274740.0
52	243221.5
53	211703.0
54	186033.0
55	160363.0
56	143475.5
57	126588.0
58	109717.0
59	92846.0
60	86960.0
61	81074.0
62	66492.5
63	51911.0
64	44496.0
65	37081.0
66	31241.0
67	25401.0
68	22373.5
69	19346.0
70	17168.0
71	14990.0
72	12849.5
73	10709.0
74	9130.0
75	6281.5
76	5012.0
77	4776.5
78	4541.0
79	4472.5
80	4404.0
81	3901.5
82	3399.0
83	3022.0
84	2645.0
85	2372.5
86	2100.0
87	2397.5
88	2695.0
89	1872.5
90	1050.0
91	719.5
92	389.0
93	306.0
94	223.0
95	173.0
96	123.0
97	107.5
98	92.0
99	110.5
100	129.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0789568040870574
2	0.47782280570926733
3	0.45614171688842825
4	0.4675199867160313
5	0.46865996460046033
6	0.4138980081144917
7	0.40219710303657846
8	0.4157908015829776
9	0.36186769674781516
10	0.3782575674635686
11	0.43734283630369275
12	0.42983618947981084
13	0.5713870282991982
14	0.6212664379971405
15	0.6839222036073632
16	0.6253101331343606
17	0.6134371559229487
18	0.5659452470773011
19	0.5925734097362285
20	0.584206402244853
21	0.5537281255988916
22	0.6898371831963819
23	0.5153775488453637
24	0.49748204696149645
25	0.5045154954182568
26	0.6012845614946014
27	0.5713009922324488
28	0.4712195375862539
29	0.41514553108235736
30	0.4215982360885595
31	0.4309976763809273
32	0.43058900506386777
33	0.566698062661358
34	0.8713732840375349
35	0.9049703681031607
36	0.9087559550401325
37	0.9093797165240656
38	0.9084333197898226
39	0.853241182970107
40	0.8857628162013657
41	0.9285657594091732
42	0.9375135103511067
43	0.872771370122212
44	0.8433685443106177
45	0.834850973702431
46	0.8206335136720989
47	0.7541276340748424
48	0.8493695599663857
49	0.7929299001788045
50	0.8258602047271227
51	0.841110097558447
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4649213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	46.61687360555737
#Duplication Level	Relative count
1	100.0
2	3.1224131842941576
3	0.823728950917795
4	0.45556899815341156
5	0.35310938865766334
6	0.2575961933650167
7	0.21939091524795803
8	0.22402185805002575
9	0.19044752273503482
10++	10.127293040271836
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15497	0.33332523160371447	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1439085	4.3354454	4.649254	43
TTTTT	1389300	3.424096	4.221155	18
TGTGT	723395	2.8020368	26.123274	4
TTTGA	822860	2.6466074	22.395777	8
TGTTT	855375	2.6428998	21.538286	6
GTTTG	664715	2.5747428	26.4239	7
CTTTG	587675	2.40838	28.033657	1
GTGTT	578210	2.2396696	25.99889	5
GAGGG	320945	2.0338407	7.872361	11
TTGTG	503945	1.9520078	25.886024	3
TTGAG	478350	1.9287841	16.25993	9
TTTGT	619605	1.914428	21.089457	2
TGAGG	355610	1.7975708	9.843034	10
TTGAC	369315	1.5755213	5.474505	9
ACTTT	417150	1.4195325	10.80082	3
TGAGT	330340	1.3319839	5.033013	10
AACTT	375485	1.3301027	11.519344	2
TTGAT	374330	1.2039772	5.4449053	9
TAACT	262890	0.9312507	11.175912	1
>>END_MODULE