FastQCFastQC Report
Tue 12 Aug 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane7_Undetermined_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4649213
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1130910 3.4066231 4.252927 16
TGTGT 607510 2.6501606 31.795118 4
TGTTT 664710 2.4095707 26.869154 6
GTTTG 517360 2.2568965 31.56384 7
TTTGA 582685 2.0494006 25.703983 8
CTTTG 447725 2.0319839 32.633476 1
GTGTT 447025 1.9500716 31.50448 5
TTTGT 532135 1.9289869 26.368784 2
TTGTG 398880 1.7400472 31.428755 3
GAGGG 275305 1.6874895 7.5177803 11
TTGAG 389665 1.6492851 17.925661 9
TGAGG 303930 1.5480653 10.228519 10
TTGAC 298440 1.31417 5.743687 9
TGAGT 271135 1.1475984 5.4602356 10
TTGAT 300930 1.0584211 6.1768103 9