##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane7_Undetermined_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4649213	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92820827955183	34.0	31.0	34.0	31.0	34.0
2	33.122343932188095	34.0	33.0	34.0	31.0	34.0
3	33.16670929036807	34.0	33.0	34.0	31.0	34.0
4	36.503509303617626	37.0	37.0	37.0	35.0	37.0
5	36.41910964285783	37.0	37.0	37.0	35.0	37.0
6	36.405107918264875	37.0	37.0	37.0	35.0	37.0
7	36.402425958974135	37.0	37.0	37.0	35.0	37.0
8	36.38728726775908	37.0	37.0	37.0	35.0	37.0
9	38.17114100816633	39.0	39.0	39.0	37.0	39.0
10	38.137680506356666	39.0	38.0	39.0	37.0	39.0
11	38.12966108457496	39.0	38.0	39.0	37.0	39.0
12	38.094683121638006	39.0	38.0	39.0	37.0	39.0
13	38.090677927640655	39.0	38.0	39.0	37.0	39.0
14	39.61991330575734	41.0	40.0	41.0	37.0	41.0
15	39.60279148320372	41.0	40.0	41.0	37.0	41.0
16	39.5379235152272	41.0	40.0	41.0	37.0	41.0
17	39.52885832505415	41.0	40.0	41.0	37.0	41.0
18	39.502375133167696	41.0	40.0	41.0	37.0	41.0
19	39.48914816335582	41.0	40.0	41.0	37.0	41.0
20	39.4555900106104	41.0	39.0	41.0	37.0	41.0
21	39.42374634158512	41.0	39.0	41.0	37.0	41.0
22	39.3754777421469	41.0	39.0	41.0	37.0	41.0
23	39.31200398863206	41.0	39.0	41.0	36.0	41.0
24	39.28391041666622	41.0	39.0	41.0	36.0	41.0
25	39.24496511560129	40.0	39.0	41.0	36.0	41.0
26	39.142635323440764	40.0	39.0	41.0	36.0	41.0
27	39.04088068238646	40.0	39.0	41.0	36.0	41.0
28	38.98861678309856	40.0	39.0	41.0	36.0	41.0
29	38.91677559191201	40.0	39.0	41.0	36.0	41.0
30	38.8570779613668	40.0	39.0	41.0	35.0	41.0
31	38.76656801914647	40.0	38.0	41.0	35.0	41.0
32	38.65075336406398	40.0	38.0	41.0	35.0	41.0
33	38.49893003396489	40.0	38.0	41.0	35.0	41.0
34	38.42764592631054	40.0	38.0	41.0	35.0	41.0
35	38.32954545210125	40.0	38.0	41.0	34.0	41.0
36	38.24165444775277	40.0	38.0	41.0	34.0	41.0
37	38.43875619379022	40.0	38.0	41.0	35.0	41.0
38	38.46396476134778	40.0	38.0	41.0	35.0	41.0
39	38.471876638046055	40.0	38.0	41.0	35.0	41.0
40	38.44655213688854	40.0	38.0	41.0	35.0	41.0
41	38.35707118602654	40.0	38.0	41.0	35.0	41.0
42	38.28923239266517	40.0	38.0	41.0	34.0	41.0
43	38.22545966381837	40.0	38.0	41.0	34.0	41.0
44	38.163379049314365	40.0	38.0	41.0	34.0	41.0
45	38.10000810889929	40.0	38.0	41.0	34.0	41.0
46	38.040042906186486	40.0	38.0	41.0	34.0	41.0
47	37.92699280501883	40.0	38.0	41.0	33.0	41.0
48	37.86146408004968	40.0	38.0	41.0	33.0	41.0
49	37.81633558195763	40.0	37.0	41.0	33.0	41.0
50	37.90687327080949	40.0	37.0	41.0	34.0	41.0
51	37.631085089024744	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	230.0
7	18.0
8	9.0
9	47.0
10	20.0
11	11.0
12	30.0
13	37.0
14	50.0
15	98.0
16	174.0
17	360.0
18	700.0
19	1251.0
20	2305.0
21	4033.0
22	6230.0
23	9137.0
24	12773.0
25	16093.0
26	18084.0
27	21059.0
28	23587.0
29	27470.0
30	31565.0
31	38173.0
32	47249.0
33	61805.0
34	86905.0
35	132079.0
36	213518.0
37	398411.0
38	996670.0
39	2497841.0
40	1191.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.21445864095107	28.65098777695301	27.267586777422707	23.866966804673215
2	22.481428699286063	28.314671671795722	29.713650852661942	19.490248776256276
3	21.060231054159058	27.048319790897946	30.87027847508815	21.021170679854848
4	21.89127493190783	26.985233844953978	30.15501763416733	20.968473588970866
5	25.19198410569703	27.36028656893113	27.402207642454755	20.045521682917087
6	22.250066839269355	26.78812521603119	29.72754743652313	21.23426050817633
7	25.19656552625143	27.537606902501565	26.733642876762154	20.53218469448485
8	22.136434704110137	27.465960367916033	29.479978654451838	20.917626273521993
9	21.524847323622296	27.467487508100835	29.953951346174073	21.053713822102797
10	21.900459282033324	27.62523463648579	29.430249807870705	21.044056273610177
11	24.877973971078546	27.420081635321935	26.685376643315763	21.01656775028376
12	21.92701891743652	30.363980512566812	26.531774625469172	21.177225944527496
13	23.482038788070152	28.012891644241723	27.03954411208951	21.465525455598613
14	23.01131016895671	28.10214448615551	27.33024475934353	21.556300585544243
15	23.06965625395905	27.82094568071344	27.284138916307228	21.825259149020283
16	23.02556152649031	28.106767458742766	27.127578637813343	21.740092376953584
17	23.0699574615994	28.173382537381077	26.988858969833807	21.767801031185723
18	22.737643108969845	27.869530176050894	27.29564242368579	22.097184291293473
19	22.58731139686404	28.311222842471327	27.354598955569433	21.7468668050952
20	22.537001969365992	28.3072271171975	27.37956453695809	21.77620637647842
21	22.5576887959317	28.123103845747654	27.27102414967867	22.048183208641976
22	22.534200949709124	28.391450337938917	27.245966145237915	21.828382567114048
23	22.7186334899872	28.275911633190248	27.228573561868235	21.776881314954316
24	22.447206633555673	28.228703752099012	27.271358516531087	22.05273109781423
25	22.539819651158087	28.532297516224254	27.031935734485756	21.8959470981319
26	22.622794570887315	28.30281599989673	27.055049533069198	22.019339896146757
27	22.647736040119586	28.196469903420912	27.006345321907332	22.14944873455217
28	22.585428361691122	28.47324476741334	26.906244164141558	22.035082706753975
29	22.81894034424788	28.49992945034939	26.766946112112	21.91418409329073
30	22.552066775561684	28.274905864026916	26.970875816228684	22.202151544182712
31	22.58675334058102	28.41981540556107	26.80074674278962	22.192684511068286
32	22.843383801051395	28.50870391525421	26.754955020150906	21.89295726354349
33	22.725268669648926	28.285108585721936	26.78126570478042	22.20835703984872
34	22.636731158827384	28.502855111963783	26.728303499760404	22.132110229448436
35	22.861390008808506	28.346191394340753	26.787208605357065	22.005209991493672
36	22.854574460550342	28.19359725813947	26.723617452412167	22.22821082889802
37	22.84341234839538	28.336466033686534	26.702207739757235	22.11791387816085
38	22.901669968474316	28.303349641804555	26.8228630877746	21.972117301946536
39	22.748987876935836	28.095385460167417	26.8421544892232	22.313472173673553
40	22.65984344029842	28.512803457737025	26.622300473831974	22.20505262813258
41	22.751535289700335	28.426522052676557	26.825451952895406	21.996490704727705
42	22.726659130734056	28.20100669823938	26.83405867762928	22.23827549339729
43	22.76415065851628	28.45633689804969	26.609061770681897	22.170450672752136
44	22.843045084435833	28.395960419382888	26.699875998197275	22.061118497984
45	22.82136630689137	28.17951919213518	26.70368029605155	22.29543420492189
46	22.753223976843167	28.404908438088118	26.61697614618019	22.224891438888523
47	22.793339397263725	28.394314515233198	26.62990058360836	22.182445503894712
48	22.693830083616508	28.207888449805623	26.65862331478231	22.43965815179556
49	22.654103331158947	28.497225882687882	26.54430673340714	22.304364052746024
50	22.71845433764634	28.34340252018593	26.69766921025345	22.240473931914277
51	22.732546806597348	28.22169930698774	26.721174297699115	22.3245795887158
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.081425445624284
2	41.971677475542336
3	42.08140173401391
4	42.859748520878696
5	45.237505788614115
6	43.484327347445685
7	45.72875022073629
8	43.05406097763213
9	42.57856114572509
10	42.944515555643505
11	45.8945417213623
12	43.10424486196401
13	44.94756424366877
14	44.56761075450096
15	44.894915402979336
16	44.76565390344389
17	44.83775849278512
18	44.834827400263315
19	44.33417820195924
20	44.313208345844416
21	44.605872004573676
22	44.362583516823165
23	44.495514804941514
24	44.499937731369904
25	44.43576674928999
26	44.642134467034076
27	44.797184774671756
28	44.620511068445104
29	44.733124437538606
30	44.754218319744396
31	44.779437851649305
32	44.73634106459489
33	44.93362570949765
34	44.76884138827582
35	44.86660000030218
36	45.08278528944836
37	44.96132622655623
38	44.87378727042085
39	45.06246005060939
40	44.864896068431
41	44.74802599442803
42	44.964934624131345
43	44.93460133126842
44	44.904163582419834
45	45.11680051181327
46	44.97811541573169
47	44.97578490115844
48	45.13348823541206
49	44.958467383904974
50	44.95892826956062
51	45.05712639531315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2970.0
1	1750.0
2	530.0
3	701.0
4	872.0
5	1664.0
6	2456.0
7	3899.0
8	5342.0
9	4016.0
10	2690.0
11	2840.0
12	2990.0
13	3731.0
14	4472.0
15	6077.0
16	7682.0
17	10602.0
18	13522.0
19	17125.0
20	20728.0
21	24979.0
22	29230.0
23	35056.5
24	40883.0
25	49817.5
26	71617.0
27	84482.0
28	89755.0
29	95028.0
30	107223.0
31	119418.0
32	135903.0
33	152388.0
34	175319.5
35	198251.0
36	229552.0
37	260853.0
38	283954.0
39	307055.0
40	334706.0
41	362357.0
42	395189.5
43	428022.0
44	438470.5
45	448919.0
46	431930.5
47	414942.0
48	391450.5
49	367959.0
50	337656.0
51	307353.0
52	272920.0
53	238487.0
54	209033.0
55	179579.0
56	157389.5
57	135200.0
58	113817.0
59	92434.0
60	82348.0
61	72262.0
62	60488.0
63	48714.0
64	41756.0
65	34798.0
66	29248.5
67	23699.0
68	22111.5
69	20524.0
70	18300.5
71	16077.0
72	13842.0
73	11607.0
74	9744.5
75	6579.5
76	5277.0
77	5005.5
78	4734.0
79	4674.0
80	4614.0
81	4050.5
82	3487.0
83	3240.5
84	2994.0
85	2648.0
86	2302.0
87	2717.0
88	3132.0
89	2026.5
90	921.0
91	552.5
92	184.0
93	134.5
94	85.0
95	53.5
96	22.0
97	19.0
98	16.0
99	26.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.26316281916961
2	0.025402148707749032
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0013550680513024463
13	0.0
14	7.313065673695742E-4
15	0.0011614869011163825
16	0.06056939099155061
17	4.3018033374680834E-5
18	9.894147676176592E-4
19	0.007829282074191911
20	2.1509016687340417E-5
21	0.0
22	0.0
23	0.03897433823746083
24	4.3018033374680834E-5
25	2.1509016687340417E-5
26	0.028133793827041263
27	0.08390667409731496
28	0.02634854544199201
29	1.0754508343670209E-4
30	0.0018712844517986163
31	0.0278326675934185
32	0.13432380921244091
33	0.23436224582526116
34	0.2638511077036049
35	0.348273998201416
36	0.39139957665953357
37	0.24894535913927798
38	0.2658299372388402
39	0.19287135263538152
40	0.14279836178725303
41	0.35218863923851196
42	0.2554195731621674
43	0.28032701448610764
44	0.15798372756851536
45	0.2155418562238383
46	0.1681574924616274
47	0.2808217218699165
48	0.23690030979436733
49	0.12376288201895674
50	0.11847166391387103
51	0.1384750494330976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4649213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	48.36866632058046
#Duplication Level	Relative count
1	100.0
2	3.5939134958817305
3	0.8499593726434672
4	0.47116042252450796
5	0.3256034185436116
6	0.26130112762835617
7	0.19641427043205295
8	0.19992166811833958
9	0.19875253555624403
10++	10.81622989822701
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1130910	3.4066231	4.252927	16
TGTGT	607510	2.6501606	31.795118	4
TGTTT	664710	2.4095707	26.869154	6
GTTTG	517360	2.2568965	31.56384	7
TTTGA	582685	2.0494006	25.703983	8
CTTTG	447725	2.0319839	32.633476	1
GTGTT	447025	1.9500716	31.50448	5
TTTGT	532135	1.9289869	26.368784	2
TTGTG	398880	1.7400472	31.428755	3
GAGGG	275305	1.6874895	7.5177803	11
TTGAG	389665	1.6492851	17.925661	9
TGAGG	303930	1.5480653	10.228519	10
TTGAC	298440	1.31417	5.743687	9
TGAGT	271135	1.1475984	5.4602356	10
TTGAT	300930	1.0584211	6.1768103	9
>>END_MODULE