FastQCFastQC Report
Tue 12 Aug 2014
lane6_Undetermined_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane6_Undetermined_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4661040
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 23165 0.4969920876027668 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1640415 4.595788 4.9413066 42
TTTTT 1367480 3.443317 4.088909 16
TGTTT 744910 2.3671165 13.382534 6
TTTGA 728285 2.3642163 13.578261 8
GTTTG 550960 2.2095084 16.10441 7
CTTTG 505595 2.125155 16.907675 1
TGTGT 526665 2.1120784 15.683843 4
GAGGG 294705 1.9228822 5.2674046 11
GTGTT 448390 1.7981732 15.720638 5
TTGAG 419990 1.7206184 10.117659 9
TGAGG 322045 1.6650296 6.237942 10
TTTGT 521215 1.6562761 12.753668 2
TTGTG 372050 1.4920278 15.517438 3
ACTTT 386655 1.3155936 7.791253 3
AACTT 361795 1.2575661 8.071169 2
TAACT 239055 0.830933 7.7540693 1
Produced by FastQC (version 0.10.1)