##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane6_Undetermined_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4661040	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91871384926969	34.0	31.0	34.0	26.0	34.0
2	30.95723851329317	34.0	31.0	34.0	26.0	34.0
3	31.017542865969826	34.0	31.0	34.0	26.0	34.0
4	34.20506946947462	37.0	35.0	37.0	30.0	37.0
5	34.23416276195871	37.0	35.0	37.0	30.0	37.0
6	34.249959451109625	37.0	36.0	37.0	30.0	37.0
7	34.271787626795735	37.0	36.0	37.0	30.0	37.0
8	34.297815294440724	37.0	36.0	37.0	30.0	37.0
9	35.94540746271218	39.0	38.0	39.0	30.0	39.0
10	35.93365965535589	39.0	38.0	39.0	30.0	39.0
11	35.94608692480648	39.0	38.0	39.0	30.0	39.0
12	35.9145776479069	39.0	38.0	39.0	30.0	39.0
13	35.908761778487204	39.0	38.0	39.0	30.0	39.0
14	37.3355581586942	41.0	39.0	41.0	30.0	41.0
15	37.27483694626092	41.0	39.0	41.0	30.0	41.0
16	37.24921669841924	41.0	39.0	41.0	30.0	41.0
17	37.19425407205259	41.0	39.0	41.0	30.0	41.0
18	37.181569563874156	41.0	39.0	41.0	29.0	41.0
19	37.18017202169473	41.0	39.0	41.0	29.0	41.0
20	37.13593811681513	41.0	39.0	41.0	29.0	41.0
21	37.11366926694472	41.0	39.0	41.0	29.0	41.0
22	37.06703182122445	41.0	39.0	41.0	28.0	41.0
23	37.07463248545389	41.0	39.0	41.0	28.0	41.0
24	37.00025981326056	41.0	38.0	41.0	28.0	41.0
25	36.98148009886206	40.0	38.0	41.0	27.0	41.0
26	36.94776122925356	40.0	38.0	41.0	27.0	41.0
27	36.92266468427647	40.0	38.0	41.0	27.0	41.0
28	36.89575009010864	40.0	38.0	41.0	27.0	41.0
29	36.866999854109814	40.0	38.0	41.0	27.0	41.0
30	36.817660865386266	40.0	38.0	41.0	27.0	41.0
31	36.77826214750356	40.0	38.0	41.0	27.0	41.0
32	36.73058909599575	40.0	38.0	41.0	27.0	41.0
33	36.631240667318885	40.0	38.0	41.0	27.0	41.0
34	36.52441987195991	40.0	38.0	41.0	26.0	41.0
35	36.43905887956336	40.0	38.0	41.0	25.0	41.0
36	36.39674707790536	40.0	38.0	41.0	25.0	41.0
37	36.338216363729984	40.0	38.0	41.0	25.0	41.0
38	36.28815521857783	40.0	38.0	41.0	25.0	41.0
39	36.24809978030654	40.0	38.0	41.0	24.0	41.0
40	36.15886111254141	40.0	37.0	41.0	24.0	41.0
41	36.07396246331291	40.0	37.0	41.0	24.0	41.0
42	35.99236844137789	40.0	37.0	41.0	24.0	41.0
43	35.94766768789798	40.0	37.0	41.0	23.0	41.0
44	35.893202804524314	40.0	37.0	41.0	23.0	41.0
45	35.82328257212983	40.0	37.0	41.0	23.0	41.0
46	35.75643053910715	40.0	36.0	41.0	23.0	41.0
47	35.69161732145616	40.0	36.0	41.0	23.0	41.0
48	35.863030997373976	40.0	37.0	41.0	23.0	41.0
49	35.89987492061857	40.0	37.0	41.0	23.0	41.0
50	35.85540458781731	40.0	36.0	41.0	23.0	41.0
51	34.48198299092048	38.0	34.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24583.0
3	903.0
4	517.0
5	472.0
6	3023.0
7	14228.0
8	50661.0
9	67311.0
10	64085.0
11	39275.0
12	23227.0
13	17594.0
14	15425.0
15	13944.0
16	12910.0
17	11906.0
18	10872.0
19	10168.0
20	10244.0
21	10436.0
22	11045.0
23	11873.0
24	12930.0
25	13591.0
26	16467.0
27	16678.0
28	18147.0
29	20950.0
30	24385.0
31	30060.0
32	38331.0
33	51336.0
34	73539.0
35	112001.0
36	181727.0
37	333318.0
38	866003.0
39	2425968.0
40	907.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.77439589375552	28.429837565216936	29.855559086882643	21.9402074541449
2	22.36420021725202	30.134413894458344	28.693931967995912	18.80745392029372
3	22.018803822402205	28.753998574735522	28.75274682822542	20.474450774636853
4	21.93081786659467	26.767584883891825	29.690635013023204	21.610962236490302
5	24.113164029101764	27.185513167395563	28.92418254334427	19.777140260158404
6	21.99235692429859	26.745650869826036	30.294696456392174	20.9672957494832
7	23.701745706288353	27.034055609987433	29.178467932310376	20.085730751413834
8	22.72377790463924	27.18929659249009	29.629076877255496	20.457848625615167
9	22.275309739094425	27.1189303216455	30.003364853672004	20.602395085588075
10	22.572909040630442	27.756665146672272	29.178327267017	20.49209854568029
11	23.681601742855843	27.165285445932614	28.58825958420772	20.564853227003823
12	22.028199823535378	28.53461014908888	28.605411725621238	20.831778301754508
13	22.786752501236837	27.282797320363073	28.96127066348178	20.969179514918313
14	23.237257136249635	27.60700329506422	28.196205822214953	20.959533746471195
15	22.646002645186016	28.172289667312338	27.958000218631838	21.223707468869808
16	22.959480130749547	27.538631382354257	28.19492892811001	21.30695955878619
17	22.94082864688728	27.67296182597338	28.136157872201682	21.25005165493766
18	22.68199522047717	27.487640861525104	28.21462483816508	21.61573907983265
19	22.55850621580528	27.582005651637044	28.383938236237388	21.475549896320288
20	22.550938313126206	27.717112479548334	28.34971451588977	21.38223469143569
21	22.443114980365742	27.608343374295842	28.304475892058445	21.64406575327997
22	22.38868636028917	27.679589861712216	28.40875781471894	21.522965963279677
23	22.525803390984873	27.717964026182496	28.30258231620425	21.453650266628376
24	22.336127597130297	27.687737499964793	28.297505355293122	21.678629547611788
25	22.403640314826355	27.82361993231484	28.17544958052411	21.597290172334695
26	22.366859961773745	27.833954461291317	28.22329274201	21.57589283492494
27	22.392554994466714	27.911029770767552	28.000461864001807	21.695953370763924
28	22.38894516520635	27.903308665647568	27.99118472970762	21.716561439438458
29	22.51303755693234	27.821596850469206	27.950379642513827	21.71498595008463
30	22.299996931556862	27.84975173701973	27.99092173006603	21.859329601357373
31	22.206551834990822	27.81939504239646	28.180296446854918	21.793756675757795
32	22.509307333759626	27.86558009408719	27.91813177999975	21.706980792153434
33	22.428418834411957	27.801406402286823	27.883910544813105	21.886264218488115
34	22.381616967657912	27.777742824904255	28.002545244144493	21.838094963293337
35	22.49933764479519	27.8490611929343	27.871298954564605	21.78030220770591
36	22.378549026036413	27.82364761657654	27.870502956684867	21.92730040070218
37	22.471746624243078	27.725368435660226	27.861773403572155	21.941111536524545
38	22.533349435357614	27.777707789962413	27.974190134787847	21.71475263989213
39	22.368292795709873	27.726045454732944	27.88821476339889	22.017446986158294
40	22.335647069794902	27.83900811420008	27.871832724049156	21.95351209195586
41	22.470343411757312	27.8644843018322	27.822042424048714	21.843129862361774
42	22.27544631457418	27.786739087973526	27.91996244950019	22.0178521479521
43	22.34535408172046	27.893110980344776	27.913232963894295	21.848301974040467
44	22.510379257211397	27.795926665557463	27.82839917687071	21.865294900360436
45	22.462156296104503	27.72369534580222	27.84307830792463	21.97107005016865
46	22.337799733232536	27.763302529077222	27.926426054578712	21.972471683111532
47	22.44970920521301	27.85339332217171	27.782781188997184	21.914116283618092
48	22.387922541063297	27.710916139740664	27.76370831346385	22.137453005732187
49	22.265241261006615	27.836481896926756	27.87449097697019	22.023785865096436
50	22.31147379335539	27.80845981529095	27.953175069621004	21.926891321732654
51	22.415771845940025	27.366661133370872	27.97191689640694	22.24565012428216
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.71460334790042
2	41.171654137545744
3	42.49325459703906
4	43.541780103084974
5	43.890304289260165
6	42.95965267378179
7	43.78747645770218
8	43.181626530254405
9	42.877704824682496
10	43.065007586310735
11	44.24645496985967
12	42.859978125289885
13	43.75593201615515
14	44.196790882720826
15	43.86971011405582
16	44.26643968953574
17	44.19088030182494
18	44.29773430030982
19	44.03405611212557
20	43.9331730045619
21	44.08718073364572
22	43.91165232356885
23	43.97945365761325
24	44.014757144742084
25	44.00093048716105
26	43.942752796698684
27	44.08850836523063
28	44.10550660464481
29	44.228023507016964
30	44.15932653291424
31	44.00030851074862
32	44.21628812591306
33	44.31468305290007
34	44.21971193095125
35	44.2796398525011
36	44.30584942673859
37	44.41285816076762
38	44.24810207524974
39	44.38573978186817
40	44.28915916175076
41	44.31347327411908
42	44.29329846252628
43	44.193656055760925
44	44.37567415757183
45	44.433226346273145
46	44.31027141634407
47	44.363825488831104
48	44.52537554679548
49	44.28902712610305
50	44.238365115088044
51	44.661421970222186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26609.0
1	14463.0
2	2317.0
3	2449.5
4	2582.0
5	3920.0
6	5258.0
7	6048.5
8	6839.0
9	5981.0
10	5123.0
11	5387.0
12	5651.0
13	6468.5
14	7286.0
15	8907.0
16	10528.0
17	13398.5
18	16269.0
19	19835.0
20	23401.0
21	26967.5
22	30534.0
23	35754.0
24	40974.0
25	49714.5
26	70317.0
27	82179.0
28	88915.5
29	95652.0
30	110733.5
31	125815.0
32	138482.0
33	151149.0
34	180478.0
35	209807.0
36	238920.0
37	268033.0
38	303745.0
39	339457.0
40	362356.0
41	385255.0
42	416844.5
43	448434.0
44	461552.0
45	474670.0
46	457145.0
47	439620.0
48	387441.0
49	335262.0
50	302519.0
51	269776.0
52	237091.5
53	204407.0
54	177904.5
55	151402.0
56	134943.5
57	118485.0
58	102157.5
59	85830.0
60	81123.5
61	76417.0
62	61475.5
63	46534.0
64	39509.5
65	32485.0
66	27123.5
67	21762.0
68	18264.5
69	14767.0
70	12880.5
71	10994.0
72	9339.0
73	7684.0
74	6511.5
75	4569.5
76	3800.0
77	3457.5
78	3115.0
79	3027.0
80	2939.0
81	2584.0
82	2229.0
83	1972.5
84	1716.0
85	1514.0
86	1312.0
87	1445.5
88	1579.0
89	1127.5
90	676.0
91	451.5
92	227.0
93	191.0
94	155.0
95	121.5
96	88.0
97	84.5
98	81.0
99	81.5
100	82.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0374937782125877
2	0.6142835075433809
3	0.5902974443471843
4	0.5875727305494053
5	0.5812865798190961
6	0.5745498858623826
7	0.5641873916550812
8	0.5215788751008359
9	0.53378645109246
10	0.5376053412972213
11	0.5554339803992242
12	0.5481609254586959
13	0.5628143075365155
14	0.5497056450920824
15	0.6919914868784649
16	0.7135961073065239
17	0.8372165868561522
18	0.7880001029813088
19	0.8598510203731357
20	0.9320881176733089
21	0.8753196711463536
22	0.9704057463570361
23	0.7093481283147108
24	0.9764558982544669
25	0.7617613236530903
26	0.8497888883167705
27	0.7788390573777526
28	0.7309312943034173
29	0.660002917803752
30	0.7141324682903387
31	0.6940940219350188
32	0.753437001184285
33	0.8984690112077991
34	1.10831488251549
35	1.2070267579767606
36	1.1880395791497178
37	1.209365291866193
38	1.2242761272162437
39	1.1744803734788802
40	1.3051379091361586
41	1.3260774419442871
42	1.362313990010813
43	1.2681290012529391
44	1.226035391243156
45	1.2307982767794312
46	1.2396375057926985
47	1.1624658874414293
48	1.2461811097952389
49	1.220264147057309
50	1.219169970650327
51	1.1279671489624632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4661040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	53.552333106404504
#Duplication Level	Relative count
1	100.0
2	3.4979984126340424
3	1.0184632675406835
4	0.5972898912596386
5	0.4304426665430761
6	0.3163145301918164
7	0.2543261515644824
8	0.21377300666809568
9	0.1813304907509863
10++	9.356189858237792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	23165	0.4969920876027668	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1640415	4.595788	4.9413066	42
TTTTT	1367480	3.443317	4.088909	16
TGTTT	744910	2.3671165	13.382534	6
TTTGA	728285	2.3642163	13.578261	8
GTTTG	550960	2.2095084	16.10441	7
CTTTG	505595	2.125155	16.907675	1
TGTGT	526665	2.1120784	15.683843	4
GAGGG	294705	1.9228822	5.2674046	11
GTGTT	448390	1.7981732	15.720638	5
TTGAG	419990	1.7206184	10.117659	9
TGAGG	322045	1.6650296	6.237942	10
TTTGT	521215	1.6562761	12.753668	2
TTGTG	372050	1.4920278	15.517438	3
ACTTT	386655	1.3155936	7.791253	3
AACTT	361795	1.2575661	8.071169	2
TAACT	239055	0.830933	7.7540693	1
>>END_MODULE