FastQCFastQC Report
Tue 12 Aug 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane6_Undetermined_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4661040
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1130230 3.5051503 4.1647015 16
TGTTT 579390 2.170607 17.613867 6
TGTGT 467915 2.1176178 20.50642 4
GTTTG 447480 2.0251362 20.513824 7
TTTGA 504760 1.7925926 16.356367 8
CTTTG 369735 1.7306529 20.888222 1
TTTGT 454515 1.7027797 16.997623 2
GTGTT 364230 1.6483765 20.277254 5
GAGGG 255095 1.5970184 5.3337474 11
TTGAG 349835 1.5008261 11.93666 9
TGAGG 281480 1.4587642 6.9352326 10
TTGTG 316525 1.4324805 20.153164 3
Produced by FastQC (version 0.10.1)