##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane6_Undetermined_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4661040	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96151095034585	34.0	33.0	34.0	31.0	34.0
2	33.191398700719155	34.0	34.0	34.0	31.0	34.0
3	33.23491431096922	34.0	34.0	34.0	31.0	34.0
4	36.55210060415701	37.0	37.0	37.0	35.0	37.0
5	36.48792608516554	37.0	37.0	37.0	35.0	37.0
6	36.46634227554365	37.0	37.0	37.0	35.0	37.0
7	36.47007491890222	37.0	37.0	37.0	35.0	37.0
8	36.45774247807356	37.0	37.0	37.0	35.0	37.0
9	38.266216767073445	39.0	39.0	39.0	37.0	39.0
10	38.24274518133292	39.0	39.0	39.0	37.0	39.0
11	38.2157675111134	39.0	39.0	39.0	37.0	39.0
12	38.18158415289292	39.0	39.0	39.0	37.0	39.0
13	38.17635828055541	39.0	39.0	39.0	37.0	39.0
14	39.75039283078455	41.0	40.0	41.0	38.0	41.0
15	39.726824700066935	41.0	40.0	41.0	38.0	41.0
16	39.70512525101694	41.0	40.0	41.0	38.0	41.0
17	39.68629898048504	41.0	40.0	41.0	38.0	41.0
18	39.655479034721864	41.0	40.0	41.0	37.0	41.0
19	39.65022441343563	41.0	40.0	41.0	37.0	41.0
20	39.627108542299574	41.0	40.0	41.0	37.0	41.0
21	39.56289218715136	41.0	40.0	41.0	37.0	41.0
22	39.52205409093249	41.0	40.0	41.0	37.0	41.0
23	39.443566242726945	41.0	39.0	41.0	37.0	41.0
24	39.434212107169216	41.0	39.0	41.0	37.0	41.0
25	39.37515018107547	41.0	39.0	41.0	37.0	41.0
26	39.2676398400357	41.0	39.0	41.0	36.0	41.0
27	39.17713986578103	40.0	39.0	41.0	36.0	41.0
28	39.13129344523969	40.0	39.0	41.0	36.0	41.0
29	39.0718517755694	40.0	39.0	41.0	36.0	41.0
30	39.0102597274428	40.0	39.0	41.0	36.0	41.0
31	38.91138286734291	40.0	39.0	41.0	36.0	41.0
32	38.852311715840244	40.0	39.0	41.0	35.0	41.0
33	38.751696617064006	40.0	38.0	41.0	35.0	41.0
34	38.67875409779791	40.0	38.0	41.0	35.0	41.0
35	38.580370904347525	40.0	38.0	41.0	35.0	41.0
36	38.4593020012701	40.0	38.0	41.0	35.0	41.0
37	38.65329325644062	40.0	38.0	41.0	35.0	41.0
38	38.68451697475241	40.0	38.0	41.0	35.0	41.0
39	38.678897413452795	40.0	38.0	41.0	35.0	41.0
40	38.656526869539846	40.0	38.0	41.0	35.0	41.0
41	38.56503634382026	40.0	38.0	41.0	35.0	41.0
42	38.51460532413367	40.0	38.0	41.0	35.0	41.0
43	38.46832788390574	40.0	38.0	41.0	35.0	41.0
44	38.40730824022106	40.0	38.0	41.0	35.0	41.0
45	38.344410904004256	40.0	38.0	41.0	35.0	41.0
46	38.28738972418173	40.0	38.0	41.0	34.0	41.0
47	38.16700049774299	40.0	38.0	41.0	34.0	41.0
48	38.091290355800425	40.0	38.0	41.0	34.0	41.0
49	38.019550786948834	40.0	38.0	41.0	34.0	41.0
50	38.105208065152844	40.0	38.0	41.0	34.0	41.0
51	37.80543676947634	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	8.0
11	10.0
12	22.0
13	36.0
14	65.0
15	142.0
16	310.0
17	599.0
18	1619.0
19	2548.0
20	3246.0
21	4209.0
22	5273.0
23	6909.0
24	9432.0
25	12163.0
26	14231.0
27	16876.0
28	19229.0
29	22296.0
30	26214.0
31	32566.0
32	40507.0
33	53355.0
34	76415.0
35	117794.0
36	194324.0
37	377113.0
38	989366.0
39	2632585.0
40	1562.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.6931602523901	30.066730399801312	26.3547846553224	22.88532469248619
2	22.835470828665574	29.384272174095706	28.34552475615903	19.43473224107969
3	21.20386643987855	27.844884995425907	29.756328641382424	21.194919923313122
4	21.800864184817122	27.78933456910904	29.278530113451072	21.13127113262276
5	23.91521457564057	28.30410914995955	27.622479718569064	20.158196555830816
6	22.397276144379795	27.560651700049775	28.57868630176956	21.46338585380087
7	24.111420330726006	28.3383435207024	26.875644868078414	20.674591280493182
8	22.176595781199048	28.210935756826807	28.45575236427922	21.156716097694936
9	21.678745516022175	28.125868904793784	28.982737758096906	21.21264782108714
10	21.99322468805245	28.406986423630777	28.407458421296532	21.192330467020234
11	23.684134871187545	28.19212021351458	26.975717865540737	21.148027049757136
12	22.037558937278398	29.789461826881297	26.852333621202938	21.320645614637364
13	22.916859756620838	28.66561969002626	27.056708374096765	21.36081217925613
14	22.605600231022297	28.62415152728307	27.318858396291628	21.451389845403003
15	22.568332389338003	28.306214921991657	27.344927312359474	21.780525376310866
16	22.59496554051894	28.61577791267318	27.150367532750934	21.638889014056943
17	22.710167687897982	28.700354427338105	26.964089559411637	21.625388325352283
18	22.47077905360177	28.309475996773255	27.219590477661637	22.000154471963338
19	22.31741072769641	28.689163730618866	27.260399188062983	21.733026353621746
20	22.372517721366904	28.712840911041315	27.16112713042583	21.753514237165955
21	22.315105641659372	28.542106482673397	27.124075313663905	22.01871256200333
22	22.35147091636201	28.769459176492802	27.135424712081424	21.743645195063763
23	22.514074920646372	28.674785314108508	27.12640982741593	21.684729937829193
24	22.242689701450676	28.518463757361374	27.23418427641226	22.004662264775696
25	22.385966523683994	28.85767801514752	26.95932333169949	21.797032129468995
26	22.492228101723242	28.65109494069295	26.968046594866575	21.888630362717233
27	22.422926389715265	28.483380680352592	26.981545840054206	22.11214708987794
28	22.396850462035776	28.821980087038916	26.78441273434484	21.996756716580464
29	22.570560928042706	28.81627683525648	26.789169254933565	21.823992981767244
30	22.41478577713142	28.61451093722384	26.890071982800922	22.080631302843816
31	22.351814158112482	28.787833836824234	26.77621355925499	22.0841384458083
32	22.612861034227947	28.857490523512926	26.752239579517077	21.77740886274205
33	22.540174916879856	28.580747958722334	26.767741510861647	22.111335613536163
34	22.463979866956603	28.79836702852905	26.749024816461652	21.98862828805269
35	22.63978518934818	28.689388017889407	26.829518807775045	21.84130798498737
36	22.644033327748954	28.52468673032859	26.670004357540865	22.161275584381592
37	22.638756597548316	28.788536261131227	26.641163360776954	21.931543780543507
38	22.66697424385659	28.69949342381444	26.78303767799037	21.850494654338593
39	22.608935640492113	28.40170651334749	26.826668605527864	22.162689240632535
40	22.513997944581106	28.892764035201466	26.501979399791015	22.09125862042641
41	22.521785946731317	28.870491651763526	26.73904627223272	21.86867612927244
42	22.52179071273108	28.575644164807006	26.765878550568917	22.136686571892994
43	22.567584816951484	28.86239929086823	26.558194589770583	22.0118213024097
44	22.628240678271762	28.818372234707653	26.63392120645156	21.919465880569025
45	22.584546620909336	28.58991689960383	26.692921649242436	22.1326148302444
46	22.59081615186045	28.763523178967453	26.555558040533622	22.090102628638476
47	22.58801912454769	28.77593765704052	26.571546314330362	22.06449690408143
48	22.50690684434052	28.654407251131914	26.622193677471678	22.21649222705589
49	22.42546735994585	28.95362879671797	26.480191003908676	22.140712839427497
50	22.491425861808192	28.75499490209637	26.654989932205087	22.09858930389035
51	22.557418953115114	28.552936665128648	26.69995045756241	22.189693924193826
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.57848494487629
2	42.270203069745264
3	42.39878636319167
4	42.93213531743989
5	44.07341113147139
6	43.86066199818066
7	44.78601161121919
8	43.33331187889398
9	42.891393337109314
10	43.185555155072684
11	44.83216192094468
12	43.35820455191576
13	44.277671935876974
14	44.0569900764253
15	44.348857765648866
16	44.233854554575885
17	44.33555601325026
18	44.47093352556511
19	44.05043708131816
20	44.126031958532856
21	44.3338182036627
22	44.095116111425774
23	44.19880485847557
24	44.247351966226375
25	44.182998653152985
26	44.38085846444048
27	44.53507347959321
28	44.39360717861624
29	44.39455390980995
30	44.49541707997523
31	44.43595260392078
32	44.39026989697
33	44.65151053041602
34	44.4526081550093
35	44.48109317433555
36	44.80530891213054
37	44.57030037809182
38	44.517468898195176
39	44.77162488112465
40	44.605256565007515
41	44.39046207600376
42	44.65847728462408
43	44.57940611936119
44	44.54770655884079
45	44.71716145115373
46	44.68091878049892
47	44.65251602862912
48	44.72339907139641
49	44.56618019937334
50	44.590015165698546
51	44.747112877308936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3700.0
1	2199.5
2	699.0
3	870.5
4	1042.0
5	1701.0
6	2360.0
7	3094.0
8	3828.0
9	3074.5
10	2321.0
11	2584.0
12	2847.0
13	3497.5
14	4148.0
15	5941.0
16	7734.0
17	10850.0
18	13966.0
19	17464.0
20	20962.0
21	24535.5
22	28109.0
23	33409.0
24	38709.0
25	47736.0
26	70807.0
27	84851.0
28	88206.0
29	91561.0
30	102702.0
31	113843.0
32	130123.5
33	146404.0
34	171728.5
35	197053.0
36	232991.5
37	268930.0
38	292939.5
39	316949.0
40	347850.5
41	378752.0
42	415171.5
43	451591.0
44	464399.0
45	477207.0
46	455361.5
47	433516.0
48	405709.0
49	377902.0
50	344366.5
51	310831.0
52	272352.5
53	233874.0
54	203263.0
55	172652.0
56	149096.5
57	125541.0
58	104425.0
59	83309.0
60	74199.5
61	65090.0
62	53119.0
63	41148.0
64	34719.0
65	28290.0
66	23677.5
67	19065.0
68	16952.5
69	14840.0
70	12809.0
71	10778.0
72	9273.0
73	7768.0
74	6470.0
75	4329.0
76	3486.0
77	3224.5
78	2963.0
79	2934.5
80	2906.0
81	2544.5
82	2183.0
83	1934.5
84	1686.0
85	1496.0
86	1306.0
87	1502.0
88	1698.0
89	1105.5
90	513.0
91	301.0
92	89.0
93	63.5
94	38.0
95	24.5
96	11.0
97	9.5
98	8.0
99	28.0
100	48.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3128486346394796
2	0.011220671781405008
3	1.716355148207267E-4
4	0.0
5	6.436331805777251E-5
6	0.0
7	0.00435525118857594
8	0.0
9	0.0
10	0.0
11	0.0
12	0.007830870363695655
13	0.0
14	3.861799083466351E-4
15	0.0
16	0.013709386746305546
17	0.0
18	0.0
19	0.012443574824502686
20	0.0
21	0.0
22	0.0
23	0.09339117450182792
24	0.023020613425329968
25	0.02720422909908518
26	0.1383811338242109
27	0.19017215042136518
28	0.1261735578325867
29	0.12308411856581364
30	0.09678097591953727
31	0.20416044487925442
32	0.15346360468908227
33	0.252003844635532
34	0.2226327171618351
35	0.3636527470264147
36	0.4956619123629062
37	0.37457305665688345
38	0.3926806034704701
39	0.2630957897808215
40	0.233703207867772
41	0.41623757787961485
42	0.32233149683332474
43	0.32951873401644266
44	0.30656248390917046
45	0.23604174175720438
46	0.23333848239877794
47	0.35481351801314726
48	0.3201860528980657
49	0.28270514734908947
50	0.280001887990663
51	0.3547920635737947
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4661040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.56450053059965
#Duplication Level	Relative count
1	100.0
2	4.174019434836827
3	1.1317380767511354
4	0.634102726994659
5	0.47175360579751063
6	0.35352109362132655
7	0.2988164984353309
8	0.24587656761017387
9	0.22117126655843392
10++	10.113291451965836
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1130230	3.5051503	4.1647015	16
TGTTT	579390	2.170607	17.613867	6
TGTGT	467915	2.1176178	20.50642	4
GTTTG	447480	2.0251362	20.513824	7
TTTGA	504760	1.7925926	16.356367	8
CTTTG	369735	1.7306529	20.888222	1
TTTGT	454515	1.7027797	16.997623	2
GTGTT	364230	1.6483765	20.277254	5
GAGGG	255095	1.5970184	5.3337474	11
TTGAG	349835	1.5008261	11.93666	9
TGAGG	281480	1.4587642	6.9352326	10
TTGTG	316525	1.4324805	20.153164	3
>>END_MODULE