FastQCFastQC Report
Tue 12 Aug 2014
lane5_Undetermined_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane5_Undetermined_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4186015
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 24281 0.5800504776022064 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1436115 4.554656 4.9015145 2
TTTGA 583965 2.0597534 8.052199 8
TGTTT 581015 1.9690632 7.7252927 6
CTTTG 397070 1.8028599 9.590178 1
GTTTG 396135 1.7518709 9.2302265 7
TTGAG 321160 1.478211 6.1618724 9
TGTGT 306885 1.3571708 8.602398 4
TTTGT 396025 1.342131 7.113394 2
GTGTT 293295 1.2970703 8.786863 5
TTGTG 229925 1.0168223 8.539439 3
Produced by FastQC (version 0.10.1)