##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane5_Undetermined_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4186015	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451916918596805	34.0	31.0	34.0	30.0	34.0
2	31.50362767453055	34.0	31.0	34.0	30.0	34.0
3	31.56388044476668	34.0	31.0	34.0	30.0	34.0
4	34.778411448597296	37.0	37.0	37.0	33.0	37.0
5	34.795964658511735	37.0	37.0	37.0	33.0	37.0
6	34.81702096146335	37.0	37.0	37.0	33.0	37.0
7	34.83173662779517	37.0	37.0	37.0	33.0	37.0
8	34.843337876237904	37.0	37.0	37.0	33.0	37.0
9	36.5586934112754	39.0	38.0	39.0	34.0	39.0
10	36.536563294684804	39.0	38.0	39.0	33.0	39.0
11	36.53698469785703	39.0	38.0	39.0	33.0	39.0
12	36.504829055796506	39.0	38.0	39.0	33.0	39.0
13	36.49097244037587	39.0	38.0	39.0	33.0	39.0
14	37.947640178069115	41.0	39.0	41.0	34.0	41.0
15	37.932544197763264	41.0	39.0	41.0	33.0	41.0
16	37.878445012738844	41.0	39.0	41.0	33.0	41.0
17	37.88972232540973	41.0	39.0	41.0	33.0	41.0
18	37.879498042888045	41.0	39.0	41.0	33.0	41.0
19	37.90542962698414	41.0	39.0	41.0	34.0	41.0
20	37.87363208206373	41.0	39.0	41.0	34.0	41.0
21	37.858946754849185	41.0	39.0	41.0	33.0	41.0
22	37.84163721343569	41.0	39.0	41.0	33.0	41.0
23	37.82187115908567	41.0	39.0	41.0	33.0	41.0
24	37.74256375096601	41.0	39.0	41.0	33.0	41.0
25	37.69895425601676	41.0	39.0	41.0	33.0	41.0
26	37.69642583698339	41.0	39.0	41.0	33.0	41.0
27	37.65688130596761	41.0	39.0	41.0	33.0	41.0
28	37.65071482065879	41.0	39.0	41.0	32.0	41.0
29	37.61009122040891	41.0	39.0	41.0	32.0	41.0
30	37.57559922742752	41.0	39.0	41.0	32.0	41.0
31	37.52019880482989	40.0	39.0	41.0	32.0	41.0
32	37.47454488337954	40.0	39.0	41.0	32.0	41.0
33	37.42675074026252	40.0	38.0	41.0	32.0	41.0
34	37.33366077283526	40.0	38.0	41.0	31.0	41.0
35	37.26985856476864	40.0	38.0	41.0	31.0	41.0
36	37.22615661912344	40.0	38.0	41.0	31.0	41.0
37	37.1938872650958	40.0	38.0	41.0	31.0	41.0
38	37.153278714959214	40.0	38.0	41.0	31.0	41.0
39	37.1049879181035	40.0	38.0	41.0	30.0	41.0
40	37.053320401384134	40.0	38.0	41.0	30.0	41.0
41	36.99506714619991	40.0	38.0	41.0	30.0	41.0
42	36.93688651378459	40.0	38.0	41.0	30.0	41.0
43	36.89539072363573	40.0	38.0	41.0	30.0	41.0
44	36.835452333543955	40.0	38.0	41.0	30.0	41.0
45	36.78495323117571	40.0	38.0	41.0	30.0	41.0
46	36.70504238518018	40.0	38.0	41.0	30.0	41.0
47	36.615168603074764	40.0	37.0	41.0	29.0	41.0
48	36.74630239022077	40.0	38.0	41.0	30.0	41.0
49	36.75013921354797	40.0	37.0	41.0	30.0	41.0
50	36.69717475928777	40.0	37.0	41.0	30.0	41.0
51	35.311345754852766	38.0	35.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25174.0
3	468.0
4	400.0
5	636.0
6	4787.0
7	13944.0
8	47230.0
9	45830.0
10	31503.0
11	19060.0
12	12271.0
13	9818.0
14	8849.0
15	8198.0
16	7754.0
17	7569.0
18	7477.0
19	6524.0
20	6484.0
21	6746.0
22	7064.0
23	7635.0
24	9097.0
25	9054.0
26	10549.0
27	11239.0
28	12222.0
29	14391.0
30	17516.0
31	22319.0
32	29583.0
33	40360.0
34	59124.0
35	90425.0
36	147955.0
37	278914.0
38	746391.0
39	2400283.0
40	1172.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.334969634809454	29.021792341230864	29.841318325297795	21.801919698661887
2	22.183986926847286	30.242245750648273	28.692333661636955	18.88143366086749
3	21.570275431919022	28.668282536489297	28.934992412688214	20.826449618903464
4	21.784245283540514	27.060299375241765	29.55868320668037	21.59677213453735
5	23.37642451743266	27.55080234985131	29.16331437782761	19.909458754888412
6	22.114801751161803	27.249431872693318	29.320610872409343	21.31515550373554
7	23.089395403197948	27.503912880329445	29.008968554889343	20.39772316158327
8	22.066221427809097	27.567518972809722	29.532177096864448	20.834082502516726
9	21.845392408197313	27.25044439556794	29.859824597459944	21.044338598774804
10	22.024075607246488	27.721406639512015	29.31892063985203	20.935597113389466
11	22.716515275657812	27.501955766137264	28.889016614573027	20.892512343631896
12	21.867124710785884	28.142580904784666	28.90753254387912	21.082761840550326
13	22.162995183583774	27.55798749617554	29.120869340999572	21.15814797924111
14	22.28204810180541	27.78315774516616	28.876159465926182	21.05863468710225
15	21.994065742210985	27.925433001372042	28.75296761064948	21.327533645767495
16	22.169626559539385	27.562265803782644	28.945928893108118	21.322178743569857
17	22.267887449517712	27.66413652139505	28.775138185814313	21.292837843272928
18	22.055098724027268	27.49444106006112	28.849061562293972	21.60139865361764
19	21.98835814267777	27.529197356260006	28.983996058374967	21.49844844268726
20	22.067917854789414	27.6483903572018	28.908710008902638	21.374981779106147
21	21.991593450036934	27.496901354736377	28.86733560808084	21.644169587145846
22	21.953838296364722	27.521321194400866	28.92950443175653	21.595336077477878
23	22.193530255614625	27.541384525875944	28.74756197151988	21.517523246989548
24	22.02925003805646	27.520356888774057	28.756715366872715	21.69367770629677
25	22.095893356889505	27.580104806963472	28.677598041957502	21.64640379418952
26	22.09714610752793	27.630431079738756	28.711135931454734	21.561286881278583
27	22.0913605934765	27.697193154656592	28.545653781590385	21.665792470276518
28	22.07508213575846	27.59624240191564	28.543563554236083	21.785111908089817
29	22.179054025573503	27.637515930148453	28.501230454180543	21.6821995900975
30	22.009804724497457	27.54459386414637	28.5490337393905	21.896567671965673
31	21.882286173595457	27.581904697843868	28.743666280200365	21.792142848360317
32	22.194680518107678	27.63043233991907	28.550271674262873	21.624615467710377
33	22.083966331645925	27.53476324616812	28.472380689567334	21.90888973261862
34	22.015338774565347	27.483296084647186	28.669937978460762	21.831427162326705
35	22.19429892383936	27.6120743748995	28.44212417498582	21.751502526275317
36	22.120329242078466	27.495380828272282	28.44413136590565	21.9401585637436
37	22.158819352831408	27.453909828838846	28.494907906687544	21.892362911642202
38	22.268393320710008	27.521455661120577	28.546985861249325	21.66316515692009
39	22.0935760931495	27.44450216148713	28.48483573374967	21.9770860116137
40	22.00450667790797	27.612072595388405	28.473333231755323	21.910087494948307
41	22.11317281141823	27.627353816617855	28.45519685608775	21.80427651587616
42	22.001808529497193	27.556301303239223	28.500253092554	21.941637074709586
43	22.010689617406275	27.646468478152336	28.54384607183034	21.798995832611045
44	22.130443421003655	27.628308907238203	28.446124253790327	21.795123417967815
45	22.215969485140235	27.441153760413524	28.45532893006464	21.8875478243816
46	22.05045322061038	27.481560763750856	28.589183651917295	21.878802363721473
47	22.163484424526	27.592719447959844	28.443091177187707	21.80070495032645
48	22.13502288730563	27.447882183412432	28.34003112276851	22.07706380651343
49	21.952057420967662	27.627985947656203	28.54710755278399	21.872849078592143
50	22.031632621712635	27.518682734475487	28.62787824660363	21.821806397208253
51	22.20843804471684	27.160681915352576	28.5589779008197	22.071902139110886
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.13688933347134
2	41.065420587714776
3	42.39672505082249
4	43.38101741807787
5	43.28588327232107
6	43.42995725489734
7	43.48711856478122
8	42.90030393032582
9	42.88973100697211
10	42.95967272063596
11	43.609027619289705
12	42.94988655133621
13	43.32114316282489
14	43.34068278890766
15	43.32159938797847
16	43.49180530310924
17	43.56072529279064
18	43.65649737764491
19	43.48680658536503
20	43.44289963389556
21	43.63576303718278
22	43.5491743738426
23	43.71105350260417
24	43.72292774435323
25	43.74229715107902
26	43.65843298880651
27	43.75715306375302
28	43.860194043848274
29	43.861253615671004
30	43.90637239646313
31	43.67442902195577
32	43.81929598581806
33	43.992856064264544
34	43.846765936892055
35	43.94580145011467
36	44.06048780582207
37	44.05118226447361
38	43.9315584776301
39	44.0706621047632
40	43.914594172856276
41	43.91744932729439
42	43.94344560420678
43	43.80968545001732
44	43.92556683897147
45	44.10351730952184
46	43.92925558433185
47	43.96418937485245
48	44.21208669381906
49	43.82490649955981
50	43.85343901892089
51	44.280340183827725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26509.0
1	14244.0
2	1979.0
3	2018.0
4	2057.0
5	2460.0
6	2863.0
7	3333.0
8	3803.0
9	3775.5
10	3748.0
11	4184.5
12	4621.0
13	5285.0
14	5949.0
15	7383.0
16	8817.0
17	11912.5
18	15008.0
19	17903.5
20	20799.0
21	23534.0
22	26269.0
23	30368.0
24	34467.0
25	42637.5
26	61776.5
27	72745.0
28	77937.0
29	83129.0
30	96226.5
31	109324.0
32	120358.5
33	131393.0
34	161134.5
35	190876.0
36	220097.5
37	249319.0
38	285559.0
39	321799.0
40	343162.0
41	364525.0
42	395897.5
43	427270.0
44	440418.5
45	453567.0
46	435627.0
47	417687.0
48	360908.5
49	304130.0
50	270449.0
51	236768.0
52	204853.0
53	172938.0
54	147594.0
55	122250.0
56	107923.5
57	93597.0
58	79244.5
59	64892.0
60	62872.5
61	60853.0
62	47272.0
63	33691.0
64	28046.5
65	22402.0
66	18408.0
67	14414.0
68	11347.5
69	8281.0
70	7168.5
71	6056.0
72	5119.0
73	4182.0
74	3517.5
75	2418.5
76	1984.0
77	1804.5
78	1625.0
79	1627.0
80	1629.0
81	1411.5
82	1194.0
83	1023.0
84	852.0
85	765.0
86	678.0
87	734.5
88	791.0
89	529.5
90	268.0
91	179.5
92	91.0
93	71.0
94	51.0
95	47.5
96	44.0
97	54.5
98	65.0
99	85.0
100	105.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9866663162936588
2	0.6512876805267063
3	0.6316030879010228
4	0.6327258741308859
5	0.6192524393725297
6	0.6492093315480235
7	0.6219757932066655
8	0.617556315493375
9	0.6180102077990642
10	0.6537482546049166
11	0.6640683322921681
12	0.6838962593301744
13	0.6824390261382245
14	0.8069727413781366
15	0.8248178757123421
16	0.9536277342532217
17	0.8078327478520742
18	0.826418443316615
19	0.7982054531577168
20	0.8494952837006079
21	0.7784730823945925
22	0.7460317270721677
23	0.7165048381336426
24	0.9760595697817614
25	0.9125385360539797
26	0.8432363476958397
27	0.8944784001012898
28	0.7842781260936715
29	0.819299500837909
30	0.825295657086752
31	0.9119890874734085
32	0.9261553052246587
33	0.9651422653764977
34	1.1374541180573887
35	1.2059201890103117
36	1.2459821572545726
37	1.182604457939114
38	1.1760110749722588
39	1.215929708804197
40	1.2246014407497345
41	1.2253658909487903
42	1.2222603120151265
43	1.1909178538538443
44	1.165141548704436
45	1.0894848680666458
46	1.14581529210956
47	1.2380032083019292
48	1.243760473863567
49	1.2568516835223955
50	1.2450504835744736
51	1.2144963646809674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4186015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	62.01933907687732
#Duplication Level	Relative count
1	100.0
2	3.437354233491313
3	1.0238927504016677
4	0.6063886761377706
5	0.42556622190485516
6	0.3236376410156003
7	0.24128856154010056
8	0.20040195564667063
9	0.15893948206460085
10++	8.756068205769042
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	24281	0.5800504776022064	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1436115	4.554656	4.9015145	2
TTTGA	583965	2.0597534	8.052199	8
TGTTT	581015	1.9690632	7.7252927	6
CTTTG	397070	1.8028599	9.590178	1
GTTTG	396135	1.7518709	9.2302265	7
TTGAG	321160	1.478211	6.1618724	9
TGTGT	306885	1.3571708	8.602398	4
TTTGT	396025	1.342131	7.113394	2
GTGTT	293295	1.2970703	8.786863	5
TTGTG	229925	1.0168223	8.539439	3
>>END_MODULE