FastQCFastQC Report
Tue 12 Aug 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane5_Undetermined_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4186015
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTTT 432310 1.9074539 10.53066 6
GTTTG 319340 1.7044673 12.047639 7
TTTGA 374120 1.5253497 9.458413 8
TGTGT 285155 1.5220059 11.7577095 4
TTTGT 334390 1.4754077 10.061579 2
CTTTG 257855 1.4092101 12.044956 1
TTGAG 269615 1.3297781 7.113415 9
GTGTT 239935 1.2806456 11.725543 5
TTGTG 210745 1.1248449 11.552244 3
Produced by FastQC (version 0.10.1)