##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename lane5_Undetermined_L005_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4186015 Filtered Sequences 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92338082878346 34.0 33.0 34.0 31.0 34.0 2 33.16240099474082 34.0 34.0 34.0 31.0 34.0 3 33.226925847136236 34.0 34.0 34.0 31.0 34.0 4 36.53559578740162 37.0 37.0 37.0 35.0 37.0 5 36.48550184363888 37.0 37.0 37.0 35.0 37.0 6 36.47276347552505 37.0 37.0 37.0 35.0 37.0 7 36.4613007836809 37.0 37.0 37.0 35.0 37.0 8 36.458850243011554 37.0 37.0 37.0 35.0 37.0 9 38.26584997903734 39.0 39.0 39.0 37.0 39.0 10 38.25139064241289 39.0 39.0 39.0 37.0 39.0 11 38.238607601740554 39.0 39.0 39.0 37.0 39.0 12 38.201141180812776 39.0 39.0 39.0 37.0 39.0 13 38.195811051799865 39.0 39.0 39.0 37.0 39.0 14 39.770820458120674 41.0 40.0 41.0 38.0 41.0 15 39.72333758956908 41.0 40.0 41.0 38.0 41.0 16 39.705572483615086 41.0 40.0 41.0 38.0 41.0 17 39.69930924757795 41.0 40.0 41.0 38.0 41.0 18 39.67788457518666 41.0 40.0 41.0 38.0 41.0 19 39.65979911682113 41.0 40.0 41.0 38.0 41.0 20 39.60189918096328 41.0 40.0 41.0 37.0 41.0 21 39.572730389164875 41.0 40.0 41.0 37.0 41.0 22 39.542207326060705 41.0 40.0 41.0 37.0 41.0 23 39.49601613945483 41.0 39.0 41.0 37.0 41.0 24 39.49087975078923 41.0 39.0 41.0 37.0 41.0 25 39.44979437484099 41.0 39.0 41.0 37.0 41.0 26 39.357043632189566 41.0 39.0 41.0 37.0 41.0 27 39.27058335911362 40.0 39.0 41.0 36.0 41.0 28 39.19072172459965 40.0 39.0 41.0 36.0 41.0 29 39.1344106507024 40.0 39.0 41.0 36.0 41.0 30 39.08339578334048 40.0 39.0 41.0 36.0 41.0 31 39.02209953858264 40.0 39.0 41.0 36.0 41.0 32 38.823013295461195 40.0 39.0 41.0 36.0 41.0 33 38.73476373113809 40.0 38.0 41.0 35.0 41.0 34 35.41893280363305 40.0 38.0 41.0 15.0 41.0 35 38.56839261206661 40.0 38.0 41.0 35.0 41.0 36 38.494410555146125 40.0 38.0 41.0 35.0 41.0 37 38.629083985604446 40.0 38.0 41.0 35.0 41.0 38 38.667598419977 40.0 38.0 41.0 35.0 41.0 39 38.64893317391361 40.0 38.0 41.0 35.0 41.0 40 38.62402595308426 40.0 38.0 41.0 35.0 41.0 41 38.550714223432074 40.0 38.0 41.0 35.0 41.0 42 38.48770131019597 40.0 38.0 41.0 35.0 41.0 43 38.43232644890188 40.0 38.0 41.0 35.0 41.0 44 38.35803598410421 40.0 38.0 41.0 35.0 41.0 45 38.309703381378235 40.0 38.0 41.0 35.0 41.0 46 38.23405458413312 40.0 38.0 41.0 34.0 41.0 47 38.14935756321943 40.0 38.0 41.0 34.0 41.0 48 38.08566691710374 40.0 38.0 41.0 34.0 41.0 49 38.02408663131881 40.0 38.0 41.0 34.0 41.0 50 38.14606063284532 40.0 38.0 41.0 34.0 41.0 51 37.831094489627965 40.0 37.0 41.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 5.0 8 3.0 9 4.0 10 10.0 11 13.0 12 34.0 13 41.0 14 92.0 15 171.0 16 304.0 17 616.0 18 1556.0 19 1759.0 20 3083.0 21 3179.0 22 4464.0 23 6426.0 24 8774.0 25 10087.0 26 11836.0 27 13640.0 28 15840.0 29 18542.0 30 22044.0 31 27717.0 32 35817.0 33 48425.0 34 69959.0 35 108631.0 36 188000.0 37 388815.0 38 1051920.0 39 2143410.0 40 796.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.28431813412477 30.51485445333813 26.469646550948827 22.73118086158827 2 22.715210887162186 29.894445921722124 28.01052267676981 19.37982051434588 3 21.054616950318692 28.470763614819344 28.954007281622978 21.520612153238986 4 21.598929578097344 28.48956751925997 28.416514712209445 21.494988190433236 5 23.23167653803273 29.01699063046388 27.45514646036694 20.29618637113645 6 22.473330840907163 28.36848410719981 27.52699166152056 21.631193390372466 7 23.344923513174223 29.195547555371874 26.532441952549142 20.927086978904757 8 21.96175647037492 28.873594917107486 27.78930439662271 21.375344215894884 9 21.684451328761273 28.555228327541016 28.27039821271074 21.489922130986976 10 21.84901391896589 29.147076634938003 27.553317415250543 21.450592030845566 11 22.783769288929925 28.892490829583746 26.95484846566484 21.36889141582149 12 21.926868393925965 29.67913397348074 26.88848463275932 21.50551299983397 13 22.368887832461184 29.357204883403426 26.883945709702427 21.389961574432963 14 22.187918885790676 29.327680092516268 27.04456885078646 21.439832170906598 15 22.17156412482994 28.859285024062263 27.208000926895863 21.761149924211928 16 22.178230454159586 29.1839281460532 27.02118493928507 21.616656460502142 17 22.293900045747566 29.208686543168145 26.901743065899193 21.5956703451851 18 22.065783328535613 28.826987003152162 27.086118898283928 22.021110770028297 19 22.02684216788085 29.197654356321678 27.031737287143535 21.743766188653932 20 22.230450679225946 29.12753537672464 26.96251685672412 21.6794970873253 21 22.130000575970374 28.897078874243203 26.939496582774947 22.033423967011476 22 22.10896070842091 29.211707827020923 26.89282763965288 21.78650382490529 23 22.282126212321167 29.095206332308592 27.0064876921151 21.616179763255143 24 22.073212828907685 28.896193635235424 27.017246713162756 22.01334682269414 25 22.10357448313098 29.303594551075307 26.78478085326988 21.80805011252383 26 22.35112800596968 29.03904368448397 26.810623103243564 21.799205206302787 27 22.280540636567064 28.80007019887849 26.831369430444653 22.088019734109793 28 22.222200951900092 29.20064800036372 26.56246335854664 22.014687689189543 29 22.397698474201444 29.26973372587942 26.569931298058542 21.762636501860595 30 22.238876496493358 29.0280493264106 26.68402133447607 22.04905284261998 31 22.163096184776172 29.162611882739114 26.632665873447692 22.041626059037018 32 22.421162495365042 29.25640392621724 26.636764386619816 21.685669191797903 33 22.362031418802772 28.984024292844218 26.63446366137806 22.019480626974953 34 22.277520269101757 29.175108561206358 26.56866567044195 21.978705499249937 35 22.48717332820907 29.074413912375096 26.687427940046117 21.750984819369716 36 22.535655058043115 28.86862787511717 26.52342153964477 22.072295527194942 37 22.468899314535122 29.21620985031293 26.46379918667141 21.851091648480537 38 22.545318598825954 29.134427728432243 26.627978340043647 21.69227533269816 39 22.43592557701705 28.837598484461385 26.658256070274778 22.068219868246782 40 22.325023700364103 29.39844951426841 26.27184761181369 22.004679173553797 41 22.37344525107757 29.302823034470237 26.57994386499546 21.743787849456737 42 22.39156784459186 28.977046258047352 26.596312264630264 22.035073632730523 43 22.42154662901721 29.246014452300084 26.372674371838784 21.959764546843914 44 22.440530418301634 29.261851583465653 26.497232691018535 21.80038530721417 45 22.433371652699478 28.988308797351287 26.557622297315923 22.02069725263331 46 22.475531015767093 29.168303681798758 26.37200234580276 21.984162956631398 47 22.4300569946844 29.150204361307335 26.468028179692084 21.951710464316186 48 22.309343573206974 29.089378215684768 26.468190044348983 22.133088166759272 49 22.228012969997057 29.41566482736543 26.337183501688344 22.01913870094917 50 22.348296631175625 29.22627715562347 26.50918295932269 21.91624325387821 51 22.408635451807683 28.90162394740049 26.601029801617027 22.088710799174798 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.015498995713045 2 42.095031401508066 3 42.575229103557675 4 43.09391776853058 5 43.52786290916918 6 44.104524231279626 7 44.272010492078984 8 43.3371006862698 9 43.174373459748246 10 43.29960594981145 11 44.15266070475142 12 43.43238139375994 13 43.75884940689414 14 43.62775105669727 15 43.93271404904187 16 43.79488691466173 17 43.88957039093266 18 44.08689409856391 19 43.77060835653478 20 43.90994776655124 21 44.16342454298185 22 43.8954645333262 23 43.89830597557631 24 44.08655965160182 25 43.911624595654814 26 44.150333212272464 27 44.36856037067686 28 44.236888641089635 29 44.16033497606204 30 44.28792933911333 31 44.204722243813194 32 44.106831687162945 33 44.38151204577772 34 44.25622576835169 35 44.23815814757879 36 44.60795058523806 37 44.31999096301566 38 44.23759393152411 39 44.50414544526383 40 44.329702873917896 41 44.11723310053431 42 44.426641477322384 43 44.38131117586113 44 44.240915725515805 45 44.45406890533278 46 44.45969397239849 47 44.381767459000585 48 44.442431739966246 49 44.24715167094623 50 44.264539885053836 51 44.49734625098248 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1349.0 1 815.5 2 282.0 3 371.5 4 461.0 5 634.5 6 808.0 7 1165.0 8 1522.0 9 1408.5 10 1295.0 11 1572.5 12 1850.0 13 2516.5 14 3183.0 15 4619.5 16 6056.0 17 9313.5 18 12571.0 19 15587.0 20 18603.0 21 21327.5 22 24052.0 23 28450.5 24 32849.0 25 41224.0 26 62592.0 27 75585.0 28 77308.0 29 79031.0 30 88399.0 31 97767.0 32 113431.0 33 129095.0 34 154347.0 35 179599.0 36 216347.5 37 253096.0 38 275540.5 39 297985.0 40 329704.0 41 361423.0 42 398393.5 43 435364.0 44 444426.0 45 453488.0 46 430584.5 47 407681.0 48 376902.5 49 346124.0 50 311216.0 51 276308.0 52 238438.0 53 200568.0 54 170891.0 55 141214.0 56 120308.5 57 99403.0 58 80407.0 59 61411.0 60 54554.0 61 47697.0 62 37451.5 63 27206.0 64 22973.5 65 18741.0 66 15396.5 67 12052.0 68 10292.0 69 8532.0 70 7267.5 71 6003.0 72 5136.0 73 4269.0 74 3583.5 75 2381.0 76 1864.0 77 1773.0 78 1682.0 79 1646.5 80 1611.0 81 1356.0 82 1101.0 83 949.5 84 798.0 85 742.0 86 686.0 87 746.5 88 807.0 89 509.0 90 211.0 91 128.5 92 46.0 93 34.5 94 23.0 95 22.0 96 21.0 97 27.0 98 33.0 99 72.5 100 112.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.3915418363288235 2 0.00926895866354994 3 0.003225024277266087 4 4.777813744097907E-5 5 4.777813744097907E-5 6 0.0 7 0.0 8 0.0012422315734654558 9 2.627797559253849E-4 10 0.0 11 0.0 12 0.0 13 0.0 14 0.019230700319994076 15 0.0 16 0.015193447706231345 17 0.0 18 0.0 19 0.005088371637464271 20 0.0 21 0.04233142977270746 22 6.927829928941965E-4 23 0.005805043699078957 24 0.0 25 0.00561393114931504 26 0.08471063768285589 27 0.0862873162184082 28 0.1673429263870292 29 0.0894884514269538 30 0.10126576230615512 31 0.1132341857351204 32 0.5257267353318132 33 0.42699321431002996 34 9.245212929241772 35 0.5450291028579688 36 0.6047517746591926 37 0.6052295560336023 38 0.5083354933032967 39 0.41719869613462923 40 0.3629466210703975 41 0.5370501539053252 42 0.5086221621279426 43 0.5083593823720173 44 0.5395107279835357 45 0.35666379599690873 46 0.28072044653447253 47 0.42778155357780606 48 0.2992344747928519 49 0.3031761711317327 50 0.19252200481842516 51 0.21724719094413183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4186015.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 61.46274324545307 #Duplication Level Relative count 1 100.0 2 2.0244227301123305 3 0.8492556489350417 4 0.6913094213746037 5 0.6429825905538726 6 0.5268803262650432 7 0.49977015287780385 8 0.44849656408019895 9 0.3765956694444772 10++ 11.810605971310364 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TGTTT 432310 1.9074539 10.53066 6 GTTTG 319340 1.7044673 12.047639 7 TTTGA 374120 1.5253497 9.458413 8 TGTGT 285155 1.5220059 11.7577095 4 TTTGT 334390 1.4754077 10.061579 2 CTTTG 257855 1.4092101 12.044956 1 TTGAG 269615 1.3297781 7.113415 9 GTGTT 239935 1.2806456 11.725543 5 TTGTG 210745 1.1248449 11.552244 3 >>END_MODULE