FastQCFastQC Report
Tue 12 Aug 2014
lane4_Undetermined_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane4_Undetermined_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4079676
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 23544 0.5771046524282811 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4874 0.1194702716588278 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1120605 4.4891315 4.799702 42
TTTTT 1619335 4.14229 5.0565805 16
TTTGA 675315 2.4263525 16.120958 8
TGTTT 672295 2.2082398 14.791475 6
GTTTG 505950 2.1339118 18.20844 7
CTTTG 462745 2.0196266 19.016077 1
GAGGG 248795 1.8925036 6.3553247 11
TGTGT 445225 1.8777957 17.700348 4
TTGAG 377565 1.7418941 11.836383 9
GTGTT 412810 1.7410811 17.887726 5
TGAGG 283175 1.6775187 7.4525576 10
TTTGT 463500 1.5224255 14.2699 2
TTGTG 341980 1.4423465 17.706522 3
AACTT 314400 1.27865 8.089924 2
ACTTT 340015 1.2641718 7.363446 3
TAACT 200385 0.81495655 7.708864 1
Produced by FastQC (version 0.10.1)