##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane4_Undetermined_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4079676	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7497342926252	34.0	31.0	34.0	31.0	34.0
2	31.836820374951344	34.0	31.0	34.0	30.0	34.0
3	31.91559721899484	34.0	31.0	34.0	31.0	34.0
4	35.16634017015077	37.0	37.0	37.0	35.0	37.0
5	35.194741690271485	37.0	37.0	37.0	35.0	37.0
6	35.22017581788358	37.0	37.0	37.0	35.0	37.0
7	35.233682037495136	37.0	37.0	37.0	35.0	37.0
8	35.24131597705308	37.0	37.0	37.0	35.0	37.0
9	36.97102588538894	39.0	39.0	39.0	35.0	39.0
10	36.931966411058134	39.0	38.0	39.0	35.0	39.0
11	36.94317980153326	39.0	39.0	39.0	35.0	39.0
12	36.89893462127875	39.0	38.0	39.0	35.0	39.0
13	36.91663578186111	39.0	38.0	39.0	35.0	39.0
14	38.41922348735537	41.0	40.0	41.0	36.0	41.0
15	38.340529738145875	41.0	40.0	41.0	36.0	41.0
16	38.287409588408494	41.0	40.0	41.0	36.0	41.0
17	38.28918031726049	41.0	40.0	41.0	36.0	41.0
18	38.2945432431399	41.0	40.0	41.0	35.0	41.0
19	38.31316040783631	41.0	40.0	41.0	35.0	41.0
20	38.283381082223194	41.0	40.0	41.0	35.0	41.0
21	38.26598876969642	41.0	40.0	41.0	35.0	41.0
22	38.26574659360204	41.0	39.0	41.0	35.0	41.0
23	38.22359618754038	41.0	39.0	41.0	35.0	41.0
24	38.19047247869684	41.0	39.0	41.0	34.0	41.0
25	38.17230461438604	41.0	39.0	41.0	34.0	41.0
26	38.148577975309806	41.0	39.0	41.0	34.0	41.0
27	38.09982949626392	41.0	39.0	41.0	34.0	41.0
28	38.045308745106226	41.0	39.0	41.0	34.0	41.0
29	37.97975550019168	41.0	39.0	41.0	34.0	41.0
30	37.93517573454363	41.0	39.0	41.0	34.0	41.0
31	37.88220167483888	41.0	39.0	41.0	34.0	41.0
32	37.84889829486459	41.0	39.0	41.0	34.0	41.0
33	37.789674474149415	40.0	39.0	41.0	33.0	41.0
34	37.70515281115461	40.0	38.0	41.0	33.0	41.0
35	37.64420826555834	40.0	38.0	41.0	33.0	41.0
36	37.5959546787539	40.0	38.0	41.0	33.0	41.0
37	37.56051411925849	40.0	38.0	41.0	33.0	41.0
38	37.52806644449216	40.0	38.0	41.0	33.0	41.0
39	37.46038950151924	40.0	38.0	41.0	32.0	41.0
40	37.40225620858127	40.0	38.0	41.0	32.0	41.0
41	37.32624404487023	40.0	38.0	41.0	32.0	41.0
42	37.26817227642587	40.0	38.0	41.0	32.0	41.0
43	37.220397158990075	40.0	38.0	41.0	32.0	41.0
44	37.14445926588288	40.0	38.0	41.0	31.0	41.0
45	37.087191727970556	40.0	38.0	41.0	31.0	41.0
46	37.01248628567563	40.0	37.0	41.0	31.0	41.0
47	36.90443873484071	40.0	37.0	41.0	31.0	41.0
48	37.05686162332499	40.0	37.0	41.0	32.0	41.0
49	37.073081538828085	40.0	37.0	41.0	32.0	41.0
50	37.03242659466095	40.0	37.0	41.0	32.0	41.0
51	35.638125919803436	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24583.0
3	449.0
4	318.0
5	466.0
6	2333.0
7	8050.0
8	26830.0
9	30922.0
10	26402.0
11	16545.0
12	10521.0
13	9135.0
14	8487.0
15	7603.0
16	7552.0
17	7271.0
18	7320.0
19	6909.0
20	6514.0
21	6761.0
22	6977.0
23	7236.0
24	8021.0
25	8547.0
26	9630.0
27	10587.0
28	12031.0
29	14189.0
30	16652.0
31	21060.0
32	27205.0
33	36885.0
34	54714.0
35	89129.0
36	152121.0
37	276540.0
38	727417.0
39	2384497.0
40	1267.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.212140421877287	26.984562936548173	31.148925388475142	22.654371253099402
2	22.106190345188324	28.838898545646995	29.948445659455146	19.106465449709535
3	22.249039748918886	27.463328916457908	29.381019143849706	20.9066121907735
4	21.867881717549512	25.35175446775907	30.57901900971478	22.201344804976635
5	25.00427283711205	25.697939888272398	29.23407329253606	20.063713982079488
6	21.9552053691593	25.406515295631344	31.308017231512437	21.33026210369692
7	24.149740740174348	25.687343157795034	29.826448103390184	20.336467998640433
8	22.665542572952834	25.876905325278866	30.78106836096333	20.676483740804972
9	22.563911673667373	25.7366776882174	30.76241108509486	20.936999553020367
10	22.534002562690876	26.79476006251188	29.8077981843903	20.863439190406943
11	24.220882032448387	25.886715708233528	28.883557747247117	21.00884451207096
12	22.194099601337765	27.705123328409748	28.89525606761944	21.20552100263305
13	23.140289922868497	26.010182878296444	29.40269099188321	21.446836206951854
14	23.486899103220438	26.389519183760385	28.747070415513708	21.376511297505466
15	22.849764709726156	26.969968189032194	28.48881071731604	21.691456383925612
16	23.15088795873409	26.354505240852816	28.8204173409766	21.674189459436498
17	23.100639203262975	26.430328922160474	28.8410510924783	21.627980782098252
18	22.857144268926067	26.300028214487426	28.872438398780897	21.970389117805613
19	22.643114846508784	26.401418724003467	29.13524511467204	21.820221314815715
20	22.62121009750234	26.51217666176929	29.07224374100292	21.79436949972545
21	22.575609158053215	26.38764992126222	29.026372893538756	22.010368027145812
22	22.473536507068246	26.523599365809236	29.15070719477718	21.852156932345334
23	22.639258955966472	26.565436247000513	28.961741207366853	21.833563589666166
24	22.425041489129775	26.550002743695806	28.96257648361025	22.062379283564173
25	22.420398829599627	26.7067989105552	28.8602911685616	22.012511091283578
26	22.437553822273024	26.675484736750004	28.881417361198697	22.00554407977828
27	22.513510723882433	26.775382949943033	28.65453784015096	22.05656848602357
28	22.47496221702285	26.733774931269437	28.66601083140827	22.125252020299442
29	22.614839940912827	26.67191534332923	28.582184731988626	22.131059983769326
30	22.439177159230315	26.697363064412755	28.609469495949096	22.253990280407837
31	22.34724022921388	26.62592412980869	28.797567324155544	22.229268316821884
32	22.664422767268157	26.683678946277507	28.537171509403255	22.114726777051082
33	22.485973807541555	26.65990329768701	28.54577416932733	22.308348725444105
34	22.428589549458128	26.589814051388572	28.675889112631502	22.3057072865218
35	22.699400037479066	26.647021554429422	28.45702251619383	22.196555891897678
36	22.568817524586272	26.576096141579658	28.47559413210447	22.3794922017296
37	22.628983782757793	26.451299086663514	28.55034501317386	22.369372117404833
38	22.749419416323015	26.578113982872132	28.618236853373922	22.05422974743093
39	22.533208218516695	26.500919276653946	28.49522169463161	22.47065081019775
40	22.452888922595424	26.684843410843488	28.53716616280355	22.325101503757537
41	22.613950271963752	26.627909714726194	28.493621957370657	22.264518055939394
42	22.40558958718797	26.577827789061985	28.56841184292546	22.448170780824583
43	22.435358360591174	26.6673189557265	28.588293723286597	22.309028960395732
44	22.61802494310171	26.59629806877733	28.49219772565362	22.29347926246734
45	22.68199888326394	26.4829128240175	28.477190480258407	22.357897812460152
46	22.445556659185588	26.54179881129883	28.570901461026217	22.441743068489366
47	22.655193708392645	26.628981321047227	28.3960425678703	22.31978240268983
48	22.582232638096066	26.491705769579287	28.336961617537938	22.589099974786713
49	22.404379712225044	26.73005201961798	28.507122485935366	22.35844578222161
50	22.458638860758803	26.589795941634165	28.59151794897723	22.360047248629805
51	22.64503087228898	26.30210488599381	28.53611480457462	22.516749437142593
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.866511674976685
2	41.21265579489786
3	43.15565193969238
4	44.06922652252615
5	45.06798681919154
6	43.285467472856226
7	44.48620873881478
8	43.342026313757806
9	43.50091122668774
10	43.39744175309782
11	45.22972654451935
12	43.39962060397081
13	44.58712612982035
14	44.8634104007259
15	44.541221093651764
16	44.825077418170586
17	44.72861998536123
18	44.82753338673168
19	44.4633361613245
20	44.41557959722779
21	44.58597718519903
22	44.32569343941358
23	44.47282254563264
24	44.487420772693945
25	44.432909920883205
26	44.4430979020513
27	44.570079209906005
28	44.60021423732229
29	44.74589992468215
30	44.693167439638145
31	44.57650854603577
32	44.77914954431924
33	44.79432253298566
34	44.734296835979926
35	44.89595592937675
36	44.94830972631587
37	44.99835590016262
38	44.803649163753946
39	45.003859028714444
40	44.77799042635296
41	44.87846832790315
42	44.85376036801255
43	44.7443873209869
44	44.911504205569045
45	45.03989669572409
46	44.88729972767496
47	44.974976111082476
48	45.17133261288278
49	44.76282549444666
50	44.81868610938861
51	45.16178030943157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27333.0
1	14532.0
2	1731.0
3	1854.5
4	1978.0
5	5437.0
6	8896.0
7	10332.0
8	11768.0
9	8053.0
10	4338.0
11	4381.5
12	4425.0
13	4717.0
14	5009.0
15	6208.5
16	7408.0
17	9513.5
18	11619.0
19	14078.0
20	16537.0
21	19080.0
22	21623.0
23	25008.5
24	28394.0
25	35426.5
26	51629.0
27	60799.0
28	65036.5
29	69274.0
30	82386.0
31	95498.0
32	105671.5
33	115845.0
34	143689.0
35	171533.0
36	197957.0
37	224381.0
38	259236.0
39	294091.0
40	316597.5
41	339104.0
42	371499.0
43	403894.0
44	418122.5
45	432351.0
46	417454.5
47	402558.0
48	352321.5
49	302085.0
50	271422.0
51	240759.0
52	210807.0
53	180855.0
54	156894.0
55	132933.0
56	119417.5
57	105902.0
58	91082.0
59	76262.0
60	73936.5
61	71611.0
62	58016.0
63	44421.0
64	38194.0
65	31967.0
66	26717.5
67	21468.0
68	18808.5
69	16149.0
70	14476.0
71	12803.0
72	10778.5
73	8754.0
74	7552.5
75	5436.5
76	4522.0
77	4239.0
78	3956.0
79	4052.0
80	4148.0
81	3685.0
82	3222.0
83	2897.5
84	2573.0
85	2252.0
86	1931.0
87	2222.0
88	2513.0
89	1687.0
90	861.0
91	574.5
92	288.0
93	227.0
94	166.0
95	128.5
96	91.0
97	90.5
98	90.0
99	119.5
100	149.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9355890026560932
2	0.6537528960633149
3	0.6257359653070489
4	0.6337267959514432
5	0.6126221788200827
6	0.6301480803867758
7	0.6236279547689572
8	0.6237505135211717
9	0.6324031614275252
10	0.7168706534538527
11	0.6865986416568375
12	0.7631978617909854
13	0.6519635382809811
14	0.778174541311614
15	0.9696848475222052
16	1.0499118067219064
17	0.9066161136325532
18	0.7870723067223966
19	0.8143538849653748
20	0.8994831942536613
21	0.8326396507957985
22	0.6992957283862739
23	0.802441174250112
24	0.8343309615763606
25	0.688069346683413
26	0.6899322397170756
27	0.733636690756815
28	0.7572905299342398
29	0.8697259292159476
30	0.8342084028241459
31	0.9323289398471839
32	0.899924405761634
33	0.9870636785862407
34	1.06116270017521
35	1.113421752119531
36	1.0857234741190231
37	1.0793259072534191
38	1.0277531843215
39	1.1025875584237572
40	1.0993520073652907
41	1.1062398092397534
42	1.1037886341954606
43	1.0444701981235764
44	1.0453771328899648
45	1.0079476899636148
46	1.0169189906257263
47	1.0866549206358544
48	1.1297220661640777
49	1.1182505669567877
50	1.0993520073652907
51	1.0789582309967751
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4079676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	62.08167870036102
#Duplication Level	Relative count
1	100.0
2	4.168303045449677
3	1.213300486748307
4	0.69441965082652
5	0.47068204267675867
6	0.3927309079224535
7	0.32668070977185903
8	0.25825032430052247
9	0.26420079260237783
10++	11.220798076808645
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	23544	0.5771046524282811	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4874	0.1194702716588278	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1120605	4.4891315	4.799702	42
TTTTT	1619335	4.14229	5.0565805	16
TTTGA	675315	2.4263525	16.120958	8
TGTTT	672295	2.2082398	14.791475	6
GTTTG	505950	2.1339118	18.20844	7
CTTTG	462745	2.0196266	19.016077	1
GAGGG	248795	1.8925036	6.3553247	11
TGTGT	445225	1.8777957	17.700348	4
TTGAG	377565	1.7418941	11.836383	9
GTGTT	412810	1.7410811	17.887726	5
TGAGG	283175	1.6775187	7.4525576	10
TTTGT	463500	1.5224255	14.2699	2
TTGTG	341980	1.4423465	17.706522	3
AACTT	314400	1.27865	8.089924	2
ACTTT	340015	1.2641718	7.363446	3
TAACT	200385	0.81495655	7.708864	1
>>END_MODULE