FastQCFastQC Report
Tue 12 Aug 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane4_Undetermined_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4079676
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4784 0.11726421411896436 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1197320 4.6469884 5.7506332 18
TGTTT 474240 2.1632268 20.889637 6
GTTTG 381645 2.0460017 23.889666 7
TGTGT 353435 1.8947674 23.634106 4
TTTGA 432185 1.8583692 19.412922 8
CTTTG 310515 1.7157364 24.357681 1
TTTGT 373800 1.7050738 20.45998 2
GTGTT 308495 1.653844 23.662495 5
GAGGG 223525 1.560328 5.7612658 11
TTGAG 295800 1.4948683 13.097694 9
TTGTG 264420 1.4175576 23.512274 3
TGAGG 232845 1.3829746 7.583484 10
TTGAT 250255 1.0760812 5.383074 9
Produced by FastQC (version 0.10.1)