##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane4_Undetermined_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4079676	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09082633032623	34.0	33.0	34.0	31.0	34.0
2	33.30150114862062	34.0	34.0	34.0	31.0	34.0
3	33.333735326040596	34.0	34.0	34.0	31.0	34.0
4	36.61950042111187	37.0	37.0	37.0	35.0	37.0
5	36.567655617750034	37.0	37.0	37.0	35.0	37.0
6	36.55198206916432	37.0	37.0	37.0	35.0	37.0
7	36.55463963314734	37.0	37.0	37.0	35.0	37.0
8	36.543135288194456	37.0	37.0	37.0	35.0	37.0
9	38.36428603644015	39.0	39.0	39.0	37.0	39.0
10	38.33979414051508	39.0	39.0	39.0	37.0	39.0
11	38.33391695811138	39.0	39.0	39.0	37.0	39.0
12	38.305570834546664	39.0	39.0	39.0	37.0	39.0
13	38.30361210056877	39.0	39.0	39.0	37.0	39.0
14	39.873250964047145	41.0	40.0	41.0	38.0	41.0
15	39.857862732236576	41.0	40.0	41.0	38.0	41.0
16	39.848676953758094	41.0	40.0	41.0	38.0	41.0
17	39.81690580330399	41.0	40.0	41.0	38.0	41.0
18	39.77785196667578	41.0	40.0	41.0	38.0	41.0
19	39.78718064865935	41.0	40.0	41.0	38.0	41.0
20	39.77023494022565	41.0	40.0	41.0	38.0	41.0
21	39.72894244542949	41.0	40.0	41.0	38.0	41.0
22	39.66802559811122	41.0	40.0	41.0	38.0	41.0
23	39.64857013154966	41.0	40.0	41.0	37.0	41.0
24	39.63373635553412	41.0	40.0	41.0	37.0	41.0
25	39.584540291925144	41.0	40.0	41.0	37.0	41.0
26	39.44130857450445	41.0	40.0	41.0	37.0	41.0
27	39.31684378857537	41.0	39.0	41.0	37.0	41.0
28	39.27117545609014	41.0	39.0	41.0	37.0	41.0
29	39.20777336239446	41.0	39.0	41.0	36.0	41.0
30	39.14355919440661	40.0	39.0	41.0	36.0	41.0
31	39.14044791792289	40.0	39.0	41.0	36.0	41.0
32	39.060906307265576	40.0	39.0	41.0	36.0	41.0
33	38.99949554817589	40.0	39.0	41.0	36.0	41.0
34	32.50672479873402	40.0	35.0	41.0	2.0	41.0
35	38.80736828120664	40.0	38.0	41.0	35.0	41.0
36	38.72286990437476	40.0	38.0	41.0	35.0	41.0
37	38.87197267626155	40.0	39.0	41.0	35.0	41.0
38	38.90475689736146	40.0	39.0	41.0	35.0	41.0
39	38.902379502685996	40.0	39.0	41.0	35.0	41.0
40	38.88944759338732	40.0	39.0	41.0	35.0	41.0
41	38.83422752198949	40.0	39.0	41.0	35.0	41.0
42	38.76016134614611	40.0	38.0	41.0	35.0	41.0
43	38.688148519637345	40.0	38.0	41.0	35.0	41.0
44	38.611083331127276	40.0	38.0	41.0	35.0	41.0
45	38.545274673773115	40.0	38.0	41.0	35.0	41.0
46	38.465513928066834	40.0	38.0	41.0	35.0	41.0
47	38.398025725572325	40.0	38.0	41.0	35.0	41.0
48	38.327224514887945	40.0	38.0	41.0	35.0	41.0
49	38.25710522110089	40.0	38.0	41.0	35.0	41.0
50	38.31796225974808	40.0	38.0	41.0	35.0	41.0
51	38.04228767186414	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	18.0
10	17.0
11	14.0
12	20.0
13	35.0
14	72.0
15	94.0
16	171.0
17	323.0
18	674.0
19	1556.0
20	2116.0
21	2988.0
22	3892.0
23	5187.0
24	6772.0
25	8377.0
26	10149.0
27	11781.0
28	13450.0
29	16160.0
30	19251.0
31	23544.0
32	29854.0
33	40686.0
34	61230.0
35	101453.0
36	179452.0
37	369299.0
38	1079286.0
39	2090148.0
40	1603.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.191381957594103	29.204024816388536	26.838094760192043	23.766498465825318
2	22.813433646286278	28.881020597430656	28.57239867313901	19.733147083144058
3	21.17602590839699	27.501713401594863	29.55632458444496	21.76593610556319
4	21.939462839696095	27.44541478293864	28.86013497150264	21.75498740586262
5	24.567980398443407	27.852849098800984	27.030087683433685	20.549082819321924
6	22.53056438184783	27.20626462895133	28.432614720760295	21.83055626844054
7	24.66374780742392	28.00653777407814	26.31287386547363	21.01684055302431
8	22.39614126219827	27.822577087003967	28.3476923167428	21.433589334054957
9	21.860314397515882	27.800222370600018	28.834667262792436	21.504795969091663
10	22.152556523342	28.08543004508729	28.15369583899764	21.608317592573073
11	24.296096062530456	27.832700439936897	26.334542252865177	21.536661244667467
12	22.220416507338882	29.865135158984618	26.218416330852722	21.696032002823777
13	23.26569560916112	28.356050823643837	26.583459078613103	21.794794488581935
14	22.966271786705992	28.47332256090865	26.756982139215285	21.803423513170074
15	22.90137746232789	28.06512085763673	26.898140930799407	22.135360749235968
16	22.916512954362336	28.4009107775755	26.6806402896761	22.00193597838607
17	23.028961123383326	28.465912489129035	26.54867690473459	21.956449482753044
18	22.755366542678033	28.075005974740634	26.832014805100897	22.337612677480436
19	22.585953326837192	28.48868087285003	26.874608600898704	22.050757199414072
20	22.662265336757134	28.449955339590694	26.845636761350654	22.042142562301517
21	22.612618023541582	28.24948243084063	26.778176416006705	22.359723129611083
22	22.604474461798226	28.565937592078082	26.751414049749258	22.078173896374434
23	22.82208194964502	28.43360600204526	26.78217583945392	21.9621362088558
24	22.508910056230174	28.26502698754308	26.824122598451833	22.40194035777491
25	22.544509823774906	28.70372163687005	26.590422833825823	22.161345705529218
26	22.792898693398676	28.44586662004297	26.5393125934817	22.22192209307665
27	22.706449745491426	28.26150841662154	26.598086787471832	22.433955050415204
28	22.64078651379074	28.66222264466711	26.36296808797268	22.334022753569474
29	22.915501684573318	28.691185134595187	26.327232326715126	22.066080854116368
30	22.715375075211515	28.437884499674592	26.477473384333905	22.369267040779988
31	22.617382146860056	28.59082549632736	26.349536231265454	22.44225612554713
32	22.941955128197257	28.67695264361627	26.307229799054753	22.07386242913172
33	22.819659352682724	28.387562774912517	26.356554140186795	22.436223732217968
34	22.73531097701655	28.5476520634679	26.316351237890462	22.400685721625084
35	22.953227127247807	28.459690335702113	26.349926117541372	22.237156419508707
36	22.98749778183276	28.271301358416697	26.288007572656824	22.453193287093725
37	22.94357478831179	28.61238423636848	26.206859275401662	22.237181699918064
38	22.972407441517774	28.531466814023453	26.388579848959292	22.107545895499477
39	22.89074829424558	28.177262038425603	26.4069284250528	22.525061242276013
40	22.79033785901782	28.71705993535484	26.095197823119108	22.397404382508228
41	22.82470515058567	28.65546604366588	26.337237156195233	22.182591649553217
42	22.80935298269882	28.398305888178204	26.381414874172116	22.410926254950862
43	22.887908664408805	28.72140144318801	26.073469022840527	22.317220869562654
44	22.98234962700552	28.59458174367982	26.233814269525663	22.189254359788997
45	22.91709059722232	28.34824546137595	26.277983405404598	22.456680535997133
46	22.89097575227413	28.578150013761782	26.12173267989793	22.409141554066153
47	22.881996807236277	28.59606729635335	26.190035691623976	22.331900204786397
48	22.80015068427595	28.420256973135743	26.168705035529833	22.610887307058476
49	22.732982913797237	28.76399472383671	26.078886834539983	22.42413552782607
50	22.78948343399175	28.557891779623695	26.250489183973656	22.402135602410894
51	22.888437801735346	28.26266378430637	26.317756936036947	22.531141477921338
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.95788042341942
2	42.54658072943033
3	42.94196201396018
4	43.69445024555871
5	45.11706321776533
6	44.36112065028837
7	45.68058836044823
8	43.82973059625323
9	43.365110366607546
10	43.76087411591507
11	45.83275730719792
12	43.91644851016266
13	45.060490097743056
14	44.76969529987607
15	45.03673821156386
16	44.918448932748404
17	44.98541060613637
18	45.09297922015847
19	44.636710526251264
20	44.70440789905865
21	44.97234115315267
22	44.68264835817266
23	44.78421815850082
24	44.91085041400509
25	44.705855529304124
26	45.014820786475326
27	45.14040479590663
28	44.97480926736021
29	44.981582538689686
30	45.0846421159915
31	45.059638272407184
32	45.01581755732897
33	45.255883084900695
34	45.13599669864164
35	45.190383546756514
36	45.44069106892648
37	45.180756488229854
38	45.07995333701725
39	45.41580953652159
40	45.18774224152605
41	45.00729680013889
42	45.22027923764968
43	45.20512953397146
44	45.171603986794516
45	45.373771133219456
46	45.30011730634028
47	45.21389701202268
48	45.41103799133443
49	45.157118441623304
50	45.19161903640265
51	45.41957927965669
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5144.0
1	2963.0
2	782.0
3	973.0
4	1164.0
5	2783.0
6	4402.0
7	6809.0
8	9216.0
9	5706.0
10	2196.0
11	2355.5
12	2515.0
13	2820.0
14	3125.0
15	4321.0
16	5517.0
17	7871.0
18	10225.0
19	12413.5
20	14602.0
21	17176.0
22	19750.0
23	23716.5
24	27683.0
25	34487.5
26	52230.0
27	63168.0
28	65190.0
29	67212.0
30	76054.0
31	84896.0
32	100365.5
33	115835.0
34	140133.5
35	164432.0
36	196972.5
37	229513.0
38	251724.0
39	273935.0
40	305792.0
41	337649.0
42	374071.5
43	410494.0
44	418907.0
45	427320.0
46	409513.5
47	391707.0
48	364214.5
49	336722.0
50	305220.0
51	273718.0
52	238947.5
53	204177.0
54	176796.0
55	149415.0
56	129677.5
57	109940.0
58	91790.0
59	73640.0
60	66549.5
61	59459.0
62	49263.5
63	39068.0
64	33828.5
65	28589.0
66	24136.0
67	19683.0
68	18425.5
69	17168.0
70	15239.5
71	13311.0
72	11351.5
73	9392.0
74	7930.5
75	5526.5
76	4584.0
77	4464.5
78	4345.0
79	4359.0
80	4373.0
81	3838.5
82	3304.0
83	3022.5
84	2741.0
85	2492.0
86	2243.0
87	2326.5
88	2410.0
89	1526.5
90	643.0
91	391.0
92	139.0
93	96.5
94	54.0
95	42.0
96	30.0
97	66.0
98	102.0
99	142.5
100	183.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.35046900783297497
2	0.014094256504683215
3	0.0017648460318907676
4	0.0
5	0.0
6	2.2060575398634595E-4
7	0.0
8	0.0
9	0.0
10	8.333995150595292E-4
11	0.0
12	8.824230159453838E-4
13	0.0
14	0.0030394570549229886
15	0.0
16	0.002083498787648823
17	0.0
18	2.451175044292733E-5
19	0.03328695710149532
20	0.0
21	0.012525504476335866
22	0.006324031614275252
23	0.0
24	3.9218800708683727E-4
25	0.01826125407998086
26	0.1802839245077305
27	0.24198000037257858
28	0.19950113685498552
29	0.18935327217161363
30	0.19182895896634927
31	0.07802090165983769
32	0.20999216604455842
33	0.12741207880233626
34	17.555609808229867
35	0.35382711764365604
36	0.407801992118982
37	0.23092520092281837
38	0.19428013401064204
39	0.09951770679828496
40	0.06495613867375742
41	0.1799897835024154
42	0.20006490711517289
43	0.2027857114143378
44	0.19692740305847817
45	0.13969246577424285
46	0.07770224890407965
47	0.10319446936472407
48	0.12086744143407467
49	0.07914844218021234
50	0.09831663102658152
51	0.0036522508159961722
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4079676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	63.23246009247627
#Duplication Level	Relative count
1	100.0
2	4.252756360947628
3	1.191391925010286
4	0.6893138465024478
5	0.5080410010554373
6	0.37506782824397894
7	0.3297496168822263
8	0.29754983512519156
9	0.23911319415872107
10++	11.375467344054668
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4784	0.11726421411896436	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1197320	4.6469884	5.7506332	18
TGTTT	474240	2.1632268	20.889637	6
GTTTG	381645	2.0460017	23.889666	7
TGTGT	353435	1.8947674	23.634106	4
TTTGA	432185	1.8583692	19.412922	8
CTTTG	310515	1.7157364	24.357681	1
TTTGT	373800	1.7050738	20.45998	2
GTGTT	308495	1.653844	23.662495	5
GAGGG	223525	1.560328	5.7612658	11
TTGAG	295800	1.4948683	13.097694	9
TTGTG	264420	1.4175576	23.512274	3
TGAGG	232845	1.3829746	7.583484	10
TTGAT	250255	1.0760812	5.383074	9
>>END_MODULE