FastQCFastQC Report
Tue 12 Aug 2014
lane3_Undetermined_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane3_Undetermined_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3783211
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 15300 0.40441836313121315 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 917345 3.8654177 4.083213 42
TTTTT 1134860 3.1356206 3.6203883 16
TTTGA 590955 2.293933 10.747776 8
TGTTT 580125 2.0696237 9.892136 6
GTTTG 418145 1.9261283 11.994038 7
CTTTG 400910 1.8960018 12.489966 1
TTGAG 326840 1.6381361 8.081099 9
TGAGG 249310 1.6134038 5.307975 10
TGTGT 344095 1.585027 11.425793 4
GTGTT 328180 1.5117167 11.585513 5
TTTGT 392875 1.4016004 9.252115 2
AACTT 283330 1.2285953 6.6177053 2
ACTTT 307780 1.2265923 6.009251 3
TTGTG 259550 1.1955819 11.316394 3
TAACT 177810 0.7710321 6.125526 1
Produced by FastQC (version 0.10.1)