##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane3_Undetermined_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3783211	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.804163711725305	34.0	31.0	34.0	31.0	34.0
2	31.90741832797589	34.0	31.0	34.0	31.0	34.0
3	31.97778765181218	34.0	31.0	34.0	31.0	34.0
4	35.260847729613815	37.0	37.0	37.0	35.0	37.0
5	35.28393816786851	37.0	37.0	37.0	35.0	37.0
6	35.31173148946755	37.0	37.0	37.0	35.0	37.0
7	35.32762433816142	37.0	37.0	37.0	35.0	37.0
8	35.34801971129816	37.0	37.0	37.0	35.0	37.0
9	37.07177315777523	39.0	38.0	39.0	35.0	39.0
10	37.01274710821046	39.0	38.0	39.0	35.0	39.0
11	37.02346895269653	39.0	38.0	39.0	35.0	39.0
12	36.99592753351584	39.0	38.0	39.0	35.0	39.0
13	36.98538595917595	39.0	38.0	39.0	35.0	39.0
14	38.47991269849871	41.0	40.0	41.0	36.0	41.0
15	38.4349315964666	41.0	40.0	41.0	36.0	41.0
16	38.41179225795231	41.0	40.0	41.0	36.0	41.0
17	38.406742843579174	41.0	40.0	41.0	36.0	41.0
18	38.40327753329117	41.0	40.0	41.0	36.0	41.0
19	38.42967547937453	41.0	40.0	41.0	36.0	41.0
20	38.37231679649906	41.0	40.0	41.0	35.0	41.0
21	38.36403573578106	41.0	40.0	41.0	35.0	41.0
22	38.374505942174515	41.0	39.0	41.0	35.0	41.0
23	38.33554036504969	41.0	39.0	41.0	35.0	41.0
24	38.34402680685798	41.0	39.0	41.0	35.0	41.0
25	38.296550734283656	41.0	39.0	41.0	35.0	41.0
26	38.25544649769733	41.0	39.0	41.0	34.0	41.0
27	38.19715342337501	41.0	39.0	41.0	34.0	41.0
28	38.189233431600826	41.0	39.0	41.0	34.0	41.0
29	38.147962934131876	41.0	39.0	41.0	34.0	41.0
30	38.11005994643175	41.0	39.0	41.0	34.0	41.0
31	38.06386215307579	41.0	39.0	41.0	34.0	41.0
32	38.02778274856993	40.0	39.0	41.0	34.0	41.0
33	37.96352727881157	40.0	39.0	41.0	34.0	41.0
34	37.878199497728254	40.0	39.0	41.0	33.0	41.0
35	37.825464929130305	40.0	38.0	41.0	33.0	41.0
36	37.77266269314611	40.0	38.0	41.0	33.0	41.0
37	37.71043962390678	40.0	38.0	41.0	33.0	41.0
38	37.65333416507829	40.0	38.0	41.0	33.0	41.0
39	37.6096337211961	40.0	38.0	41.0	33.0	41.0
40	37.574200064442614	40.0	38.0	41.0	33.0	41.0
41	37.50103126682598	40.0	38.0	41.0	33.0	41.0
42	37.41870596168176	40.0	38.0	41.0	32.0	41.0
43	37.367906786060836	40.0	38.0	41.0	32.0	41.0
44	37.306352196586445	40.0	38.0	41.0	32.0	41.0
45	37.24259709543031	40.0	38.0	41.0	32.0	41.0
46	37.164823479314265	40.0	38.0	41.0	31.0	41.0
47	37.07507088555198	40.0	37.0	41.0	31.0	41.0
48	37.227858028537135	40.0	38.0	41.0	32.0	41.0
49	37.2693878295448	40.0	38.0	41.0	32.0	41.0
50	37.21351782916681	40.0	37.0	41.0	32.0	41.0
51	35.75638181428422	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16243.0
3	436.0
4	472.0
5	484.0
6	1694.0
7	6494.0
8	21043.0
9	25774.0
10	22313.0
11	14344.0
12	9879.0
13	8389.0
14	7800.0
15	7444.0
16	7538.0
17	7086.0
18	6613.0
19	6167.0
20	5922.0
21	6089.0
22	6432.0
23	6764.0
24	7050.0
25	7759.0
26	9606.0
27	9967.0
28	10857.0
29	12770.0
30	15116.0
31	19074.0
32	25582.0
33	34591.0
34	51551.0
35	82774.0
36	140173.0
37	266387.0
38	718040.0
39	2175633.0
40	861.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.609834512166234	28.57994048588185	29.21161513919359	21.598609862758323
2	22.503617791132505	29.550947324323147	28.80362491507096	19.141809969473393
3	22.18740259295074	27.74160734884618	28.972952030491044	21.098038027712033
4	21.824835477370698	25.75735675478051	30.229571773528434	22.188235994320355
5	24.026952392998776	26.23646507732192	29.483996186252863	20.25258634342644
6	22.028611145718248	25.81277558609071	30.455757864903944	21.702855403287096
7	23.60870605474066	26.036614333289165	29.809365517435506	20.54531409453467
8	22.54013735739287	26.281688592677476	30.103794652635862	21.074379397293793
9	22.396979413810588	26.07630029186499	30.315341145261332	21.21137914906309
10	22.516730723285516	26.862967434618884	29.561885717599974	21.05841612449563
11	23.46811707571721	26.204884365707855	29.19626000299289	21.130738555582045
12	22.145854973675238	27.236301630332164	29.293696470902347	21.324146925090247
13	22.662289695648717	26.267305744867564	29.58394923834373	21.48645532113999
14	22.94535159651959	26.606120812947935	29.024057655277474	21.424469935254997
15	22.493057221289412	26.99461634817835	28.781588976839632	21.7307374536926
16	22.781026349566236	26.46517076802744	29.030311321537138	21.723491560869185
17	22.824010757750518	26.584880479356155	28.960026388141745	21.63108237475158
18	22.524839147663016	26.37773291868406	28.97602772281341	22.121400210839514
19	22.453900448858274	26.501552369148623	29.188104683762813	21.85644249823029
20	22.498338275420387	26.64390801375007	29.076155758095545	21.781597952733996
21	22.379353550278537	26.449630978829585	29.08403654762678	22.0869789232651
22	22.33105621583992	26.57380172338204	29.16836055511714	21.92678150566091
23	22.52526952431162	26.60534824075086	28.992337589519078	21.87704464541844
24	22.299286357991406	26.55735300019055	28.994015378396632	22.149345263421417
25	22.371321135780818	26.653964204568105	28.93854718911521	22.03616747053587
26	22.396388411633243	26.722792462601042	28.913389242913183	21.96742988285253
27	22.396764377933724	26.801473610668058	28.693930468286695	22.10783154311152
28	22.36582896998839	26.713556846082238	28.764592502159857	22.156021681769513
29	22.50853030898409	26.671113717779043	28.666730120006573	22.153625853230295
30	22.28637947270304	26.67182321968723	28.777232658145515	22.264564649464223
31	22.196577767959514	26.668469044689143	28.900653082903805	22.23430010444754
32	22.545041290120498	26.677908874829924	28.68475470953596	22.092295125513616
33	22.401150236321328	26.59432121349294	28.628432018421652	22.37609653176408
34	22.288077874693787	26.580471289353465	28.871820046085343	22.259630789867405
35	22.492466919079853	26.69076186401939	28.649882655855812	22.166888561044942
36	22.430052562228678	26.551817548222928	28.605221241816647	22.41290864773175
37	22.42800625190973	26.52552943127565	28.69666026224532	22.349804054569297
38	22.5872036122544	26.61341549725833	28.72453733457568	22.074843555911595
39	22.417550715255476	26.504766770082668	28.614005206705794	22.463677307956065
40	22.303712496135063	26.616914959148186	28.63347299924126	22.445899545475488
41	22.504817037865596	26.628462695813788	28.569619659272544	22.29710060704807
42	22.31227110746967	26.555788881682492	28.645425777791054	22.486514233056784
43	22.333922865328105	26.682338940196715	28.760559189847605	22.223179004627568
44	22.500137986127793	26.63676211751512	28.604297647943127	22.258802248413957
45	22.54432111175399	26.442552533415814	28.66806026977952	22.345066085050675
46	22.351931929994613	26.496475658303204	28.805152062128435	22.346440349573747
47	22.535849056603773	26.629028601906796	28.59505300353357	22.240069337955866
48	22.417337952290694	26.487527321004727	28.53726969246794	22.557865034236634
49	22.23740441085526	26.63781043814268	28.739598749504196	22.385186401497865
50	22.330810926134586	26.603844281914007	28.82126022574989	22.244084566201522
51	22.515149535414245	26.36513813576384	28.689653517292136	22.43005881152978
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.208444374924554
2	41.6454277606059
3	43.28544062066277
4	44.013071471691056
5	44.279538736425216
6	43.73146654900535
7	44.15402014927533
8	43.61451675468666
9	43.608358562873676
10	43.57514684778115
11	44.59885563129926
12	43.47000189876548
13	44.1487450167887
14	44.36982153177459
15	44.22379467498201
16	44.50451791043542
17	44.45509313250209
18	44.646239358502534
19	44.310342947088564
20	44.27993622815439
21	44.46633247354364
22	44.25783772150083
23	44.40231416973006
24	44.44863162141282
25	44.40748860631668
26	44.363818294485775
27	44.504595921045244
28	44.5218506517579
29	44.66215616221439
30	44.550944122167266
31	44.43087787240705
32	44.63733641563412
33	44.77724676808541
34	44.54770866456119
35	44.659355480124795
36	44.84296120996043
37	44.777810306479026
38	44.662047168166
39	44.88122802321154
40	44.749612041610554
41	44.80191764491367
42	44.79878534052645
43	44.55710186995567
44	44.758940234541754
45	44.88938719680467
46	44.69837227956836
47	44.77591839455964
48	44.97520298652733
49	44.622590812353124
50	44.574895492336104
51	44.94520834694403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18047.0
1	9701.0
2	1355.0
3	1405.5
4	1456.0
5	2646.5
6	3837.0
7	4494.0
8	5151.0
9	4260.5
10	3370.0
11	3432.5
12	3495.0
13	3874.5
14	4254.0
15	5186.5
16	6119.0
17	7922.5
18	9726.0
19	11750.0
20	13774.0
21	16084.0
22	18394.0
23	21458.0
24	24522.0
25	30978.0
26	45895.5
27	54357.0
28	58512.0
29	62667.0
30	75589.5
31	88512.0
32	98554.0
33	108596.0
34	135775.0
35	162954.0
36	188466.5
37	213979.0
38	249457.5
39	284936.0
40	306204.0
41	327472.0
42	359486.0
43	391500.0
44	405322.5
45	419145.0
46	403325.5
47	387506.0
48	335305.5
49	283105.0
50	252628.5
51	222152.0
52	192887.0
53	163622.0
54	140229.0
55	116836.0
56	104122.0
57	91408.0
58	77718.0
59	64028.0
60	62554.5
61	61081.0
62	48365.5
63	35650.0
64	30501.5
65	25353.0
66	20971.5
67	16590.0
68	14270.5
69	11951.0
70	10571.5
71	9192.0
72	7989.5
73	6787.0
74	5730.5
75	4028.0
76	3382.0
77	3228.5
78	3075.0
79	3072.5
80	3070.0
81	2631.0
82	2192.0
83	2043.0
84	1894.0
85	1666.5
86	1439.0
87	1671.5
88	1904.0
89	1272.0
90	640.0
91	435.0
92	230.0
93	185.0
94	140.0
95	119.0
96	98.0
97	86.0
98	74.0
99	80.0
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8058762781140147
2	0.5616128732973128
3	0.5288629156555106
4	0.5179462631082432
5	0.5282021013366688
6	0.49518781796732986
7	0.46040255222349485
8	0.4449130645898418
9	0.47879962286005195
10	0.6491046891119739
11	0.5540795900625157
12	0.6045922365947868
13	0.6300996693020823
14	0.752826104597391
15	0.8223437709395537
16	0.8817377619170593
17	0.7932415083377586
18	0.7334510287689479
19	0.637500789673111
20	0.8216565240479583
21	0.6793964174876844
22	0.5202194643650593
23	0.5778689055408224
24	0.5392244841749508
25	0.5329070992868228
26	0.5694104822596466
27	0.6489725262482056
28	0.534836677097841
29	0.5793226970422745
30	0.5211181718386841
31	0.5686703702225437
32	0.5916667085182401
33	0.6155617542875615
34	0.7631612405440775
35	0.8306700313569612
36	0.8619397649245575
37	0.8637371798718074
38	0.8804425658521292
39	0.8633671238532559
40	0.7479889437834686
41	0.8443356714706106
42	0.9251400463785922
43	0.8514724661141025
44	0.8678870937941341
45	0.8654288645280425
46	0.8462123841361213
47	0.8844603169106877
48	0.9298714769014998
49	0.838943426628861
50	0.8851211312295296
51	0.8748124278555968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3783211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	64.74720053595172
#Duplication Level	Relative count
1	100.0
2	3.513967262043557
3	1.015482908175162
4	0.5998461036235602
5	0.43195914750734504
6	0.37716271044163596
7	0.300797463041552
8	0.2623233689315861
9	0.2331763279391876
10++	9.85344867789022
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15300	0.40441836313121315	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	917345	3.8654177	4.083213	42
TTTTT	1134860	3.1356206	3.6203883	16
TTTGA	590955	2.293933	10.747776	8
TGTTT	580125	2.0696237	9.892136	6
GTTTG	418145	1.9261283	11.994038	7
CTTTG	400910	1.8960018	12.489966	1
TTGAG	326840	1.6381361	8.081099	9
TGAGG	249310	1.6134038	5.307975	10
TGTGT	344095	1.585027	11.425793	4
GTGTT	328180	1.5117167	11.585513	5
TTTGT	392875	1.4016004	9.252115	2
AACTT	283330	1.2285953	6.6177053	2
ACTTT	307780	1.2265923	6.009251	3
TTGTG	259550	1.1955819	11.316394	3
TAACT	177810	0.7710321	6.125526	1
>>END_MODULE