FastQCFastQC Report
Tue 12 Aug 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane3_Undetermined_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3783211
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 845210 3.6218138 4.3359866 18
TGTTT 402375 2.0189102 14.563446 6
GTTTG 314055 1.8450844 16.438044 7
TTTGA 360675 1.6673987 13.124675 8
TGTGT 278330 1.6351988 16.128048 4
TTTGT 312980 1.5703722 13.996615 2
CTTTG 252125 1.5251797 16.522655 1
GTGTT 246190 1.4463751 16.129425 5
TTGAG 256045 1.3860033 9.217945 9
TGAGG 205830 1.3046113 5.2696238 10
TTGTG 209460 1.2305851 15.975301 3