##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane3_Undetermined_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3783211	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11111328445598	34.0	33.0	34.0	31.0	34.0
2	33.30493382473248	34.0	34.0	34.0	31.0	34.0
3	33.310547574533906	34.0	34.0	34.0	31.0	34.0
4	36.60460307394962	37.0	37.0	37.0	35.0	37.0
5	36.54683468619646	37.0	37.0	37.0	35.0	37.0
6	36.53741913945588	37.0	37.0	37.0	35.0	37.0
7	36.52924803824053	37.0	37.0	37.0	35.0	37.0
8	36.51305385821727	37.0	37.0	37.0	35.0	37.0
9	38.334892502691496	39.0	39.0	39.0	37.0	39.0
10	38.29288030723108	39.0	39.0	39.0	37.0	39.0
11	38.27407247441393	39.0	39.0	39.0	37.0	39.0
12	38.235206548088385	39.0	39.0	39.0	37.0	39.0
13	38.248293050533	39.0	39.0	39.0	37.0	39.0
14	39.83888316036298	41.0	40.0	41.0	38.0	41.0
15	39.82823215517189	41.0	40.0	41.0	38.0	41.0
16	39.76263576099773	41.0	40.0	41.0	38.0	41.0
17	39.76895975402905	41.0	40.0	41.0	38.0	41.0
18	39.767726410184366	41.0	40.0	41.0	38.0	41.0
19	39.7625173430718	41.0	40.0	41.0	38.0	41.0
20	39.74973455088812	41.0	40.0	41.0	38.0	41.0
21	39.70068283265194	41.0	40.0	41.0	38.0	41.0
22	39.66297491733874	41.0	40.0	41.0	38.0	41.0
23	39.63705275756494	41.0	40.0	41.0	37.0	41.0
24	39.60540794578996	41.0	40.0	41.0	37.0	41.0
25	39.55500816634335	41.0	40.0	41.0	37.0	41.0
26	39.455028810182675	41.0	40.0	41.0	37.0	41.0
27	39.31095701508586	41.0	39.0	41.0	37.0	41.0
28	39.25510446020589	41.0	39.0	41.0	37.0	41.0
29	39.21675687663205	40.0	39.0	41.0	36.0	41.0
30	39.113610633929746	40.0	39.0	41.0	36.0	41.0
31	39.103476121210264	40.0	39.0	41.0	36.0	41.0
32	38.98932758442498	40.0	39.0	41.0	36.0	41.0
33	38.93022778798222	40.0	39.0	41.0	36.0	41.0
34	38.839326170282334	40.0	39.0	41.0	35.0	41.0
35	38.775806583349436	40.0	38.0	41.0	35.0	41.0
36	38.69363881633882	40.0	38.0	41.0	35.0	41.0
37	38.86586341602411	40.0	39.0	41.0	36.0	41.0
38	38.901091956013026	40.0	39.0	41.0	35.0	41.0
39	38.892708601238475	40.0	39.0	41.0	35.0	41.0
40	38.86517907671552	40.0	39.0	41.0	35.0	41.0
41	38.78035060693152	40.0	38.0	41.0	35.0	41.0
42	38.68595909665097	40.0	38.0	41.0	35.0	41.0
43	38.6467080477404	40.0	38.0	41.0	35.0	41.0
44	38.60390393240028	40.0	38.0	41.0	35.0	41.0
45	38.53776540615895	40.0	38.0	41.0	35.0	41.0
46	38.4474957912736	40.0	38.0	41.0	35.0	41.0
47	38.35306516078538	40.0	38.0	41.0	35.0	41.0
48	38.26816479440348	40.0	38.0	41.0	35.0	41.0
49	38.2054852346327	40.0	38.0	41.0	34.0	41.0
50	38.294719749969005	40.0	38.0	41.0	35.0	41.0
51	37.99961857797516	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	4.0
9	8.0
10	9.0
11	11.0
12	11.0
13	28.0
14	40.0
15	83.0
16	173.0
17	328.0
18	759.0
19	1434.0
20	2039.0
21	3134.0
22	3623.0
23	5065.0
24	6250.0
25	7516.0
26	9097.0
27	10309.0
28	12257.0
29	14175.0
30	17015.0
31	20996.0
32	26732.0
33	36618.0
34	54173.0
35	87167.0
36	149883.0
37	301305.0
38	818364.0
39	2193350.0
40	1249.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.6837968961561	30.937353439161853	24.87425908114138	22.504590583540672
2	23.190834306049258	29.830648284869387	27.31478849873029	19.663728910351065
3	21.432456767809523	28.082684249983142	28.752107234607436	21.732751747599902
4	21.858204578068737	28.05994696039951	28.396037122962483	21.685811338569273
5	23.795149513323086	28.71491601836325	26.998013326256448	20.49192114205722
6	22.583091453265496	27.86022244067275	27.55127853032781	22.005407575733944
7	24.027869447408566	28.500472217912243	26.3450809378594	21.12657739681979
8	22.33018459715834	28.487758150417726	27.568776893490742	21.613280358933192
9	21.991028256155946	28.228824667722733	28.079639227101	21.70050784902032
10	22.225617339344804	28.742277393462857	27.378568099955302	21.65353716723704
11	23.545184236353723	28.417949725775276	26.433180702847398	21.60368533502361
12	22.21636591101749	29.611415542373866	26.45247017532545	21.7197483712832
13	22.891887619503805	28.892706031878962	26.561896136702355	21.653510211914877
14	22.64619051030121	28.914381143778662	26.699085456030968	21.74034288988916
15	22.616804939933267	28.435701014667707	26.88241648822468	22.065077557174344
16	22.633403080358512	28.83405987953043	26.620005942065916	21.912531098045147
17	22.74321997900725	28.935288039710183	26.4677545080092	21.853737473273366
18	22.43013356742193	28.40097875254779	26.81757662793473	22.35131105209555
19	22.414541220034	28.866438157851942	26.700945633041073	22.018074989072982
20	22.555775067391075	28.835740239198	26.631144165352506	21.977340528058424
21	22.453369184315267	28.5498512186012	26.643884446138543	22.35289515094499
22	22.51048540132383	28.92596237441242	26.540046210571866	22.023506013691886
23	22.699447638527168	28.762101823028107	26.634808367812425	21.903642170632303
24	22.459572040787574	28.546306299067115	26.696449127473993	22.29767253267132
25	22.542791978328037	28.97385428099537	26.403982921274178	22.079370819402413
26	22.682096909339077	28.78224779524811	26.406436317177366	22.129218978235443
27	22.66500853812458	28.477270557788785	26.455193407082934	22.402527497003703
28	22.627077065886475	28.883415156739595	26.217973103712094	22.271534673661836
29	22.81022507046866	28.95550577660202	26.218661384232934	22.015607768696388
30	22.605970286838023	28.72944260405249	26.360802529798722	22.30378457931077
31	22.53967178598526	28.861037401027286	26.273634825586516	22.325655987400935
32	22.79089771557257	29.005782330151508	26.252049350399343	21.951270603876576
33	22.66070441950556	28.669510126616128	26.297195129990207	22.372590323888108
34	22.591647162568044	28.908455606903456	26.220669261319564	22.27922796920894
35	22.859732865424366	28.746007859894647	26.35067075379774	22.043588520883247
36	22.868097023985325	28.549748449552855	26.163714375406833	22.418440151054988
37	22.853590052783712	28.86090680088742	26.11303255017579	22.172470596153076
38	22.933260718437595	28.773625973480215	26.258223756026656	22.03488955205554
39	22.770269895110562	28.495047688194063	26.326188675134638	22.408493741560736
40	22.682399706898266	29.02228653235661	25.922931610301763	22.37238215044336
41	22.69444992930057	28.958617975279715	26.311954762968075	22.034977332451632
42	22.70071962524095	28.63464335228741	26.328319532071156	22.336317490400486
43	22.783060841711066	28.95923728844999	26.005314686352417	22.252387183486526
44	22.823672215236208	28.91088962220747	26.114002084252146	22.151436078304172
45	22.803043480447947	28.696947385615985	26.17050066942485	22.329508464511218
46	22.80347092615871	28.839178920123924	26.040397002743205	22.31695315097416
47	22.789667462648293	28.842554057254517	26.087330858319685	22.280447621777505
48	22.673433870254772	28.74826421122023	26.15099713666031	22.427304781864684
49	22.549255095870134	29.108109638121398	25.98868636676509	22.353948899243377
50	22.72005338989868	28.80032558946936	26.234879565767805	22.244741454864155
51	22.780351859224567	28.569482342888815	26.243841273158463	22.406324524728156
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.18838747969677
2	42.85456321640032
3	43.16520851540942
4	43.54401591663801
5	44.2870706553803
6	44.588499028999436
7	45.15444684422836
8	43.94346495609153
9	43.69153610517627
10	43.87915450658184
11	45.14886957137733
12	43.93611428230069
13	44.54539783141868
14	44.38653340019037
15	44.681882497107615
16	44.545934178403655
17	44.59695745228062
18	44.78144461951748
19	44.43261620910698
20	44.533115595449495
21	44.80626433526025
22	44.53399141501571
23	44.60308980915947
24	44.75724457345889
25	44.62216279773045
26	44.81131588757452
27	45.067536035128285
28	44.89861173954831
29	44.825832839165045
30	44.90975486614879
31	44.86532777338619
32	44.74216831944915
33	45.03329474339367
34	44.870875131776984
35	44.90332138630762
36	45.286537175040316
37	45.02606064893679
38	44.96815027049313
39	45.1787636366713
40	45.054781857341624
41	44.72942726175221
42	45.03703711564144
43	45.035448025197596
44	44.97510829354038
45	45.13255194495917
46	45.12042407713287
47	45.0701150844258
48	45.10073865211945
49	44.90320399511351
50	44.964794844762835
51	45.18667638395272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3162.0
1	1825.5
2	489.0
3	580.0
4	671.0
5	1232.0
6	1793.0
7	2617.5
8	3442.0
9	2532.0
10	1622.0
11	1685.5
12	1749.0
13	2140.5
14	2532.0
15	3626.5
16	4721.0
17	6509.5
18	8298.0
19	10323.0
20	12348.0
21	14597.0
22	16846.0
23	20051.5
24	23257.0
25	29561.5
26	46318.5
27	56771.0
28	57675.5
29	58580.0
30	67435.5
31	76291.0
32	90301.5
33	104312.0
34	126805.0
35	149298.0
36	182089.5
37	214881.0
38	237093.0
39	259305.0
40	289505.5
41	319706.0
42	356550.0
43	393394.0
44	406360.5
45	419327.0
46	398042.0
47	376757.0
48	351197.5
49	325638.0
50	293112.5
51	260587.0
52	225834.5
53	191082.0
54	163120.5
55	135159.0
56	115701.5
57	96244.0
58	78488.5
59	60733.0
60	55191.0
61	49649.0
62	39889.0
63	30129.0
64	25912.5
65	21696.0
66	18047.5
67	14399.0
68	13570.5
69	12742.0
70	11232.5
71	9723.0
72	8288.0
73	6853.0
74	5854.0
75	4165.0
76	3475.0
77	3285.5
78	3096.0
79	3250.0
80	3404.0
81	2817.5
82	2231.0
83	2079.0
84	1927.0
85	1683.5
86	1440.0
87	1727.5
88	2015.0
89	1271.5
90	528.0
91	314.5
92	101.0
93	71.0
94	41.0
95	28.0
96	15.0
97	12.5
98	10.0
99	15.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24267745045148154
2	0.022361956549608257
3	0.042186386114863805
4	0.0
5	7.929771826102218E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	4.7578630956613316E-4
13	0.007321822652767715
14	3.7005601855143687E-4
15	5.286514550734813E-5
16	3.964885913051109E-4
17	0.0
18	7.401120371028737E-4
19	0.03436234457977628
20	0.00182384752000351
21	0.0038062904765290653
22	0.002484661838845362
23	0.0
24	0.0
25	0.006846036343201582
26	0.09341271211148414
27	0.31430972261393825
28	0.22243009972216723
29	0.24299464132452567
30	0.21275577809432256
31	0.11942236370109942
32	0.3143890203321993
33	0.15563498837363288
34	0.33400198931542546
35	0.33508572479832605
36	0.39072629044480994
37	0.2413293892410442
38	0.115563208079063
39	0.027437010518313674
40	0.15021631095912968
41	0.26950651179646073
42	0.36342144279026467
43	0.25155879489671606
44	0.09087518512713143
45	0.1609215029243677
46	0.22272085802245764
47	0.22248296486767458
48	0.19956592429023917
49	0.07911269025174647
50	0.11151902444775086
51	0.07945631369754423
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3783211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	66.1539503323063
#Duplication Level	Relative count
1	100.0
2	3.8696010356596444
3	1.063316464634577
4	0.6366953042250206
5	0.46604684006119806
6	0.38013416499941155
7	0.33070495468989053
8	0.28480640225962106
9	0.24479227962810404
10++	10.41249852889255
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	845210	3.6218138	4.3359866	18
TGTTT	402375	2.0189102	14.563446	6
GTTTG	314055	1.8450844	16.438044	7
TTTGA	360675	1.6673987	13.124675	8
TGTGT	278330	1.6351988	16.128048	4
TTTGT	312980	1.5703722	13.996615	2
CTTTG	252125	1.5251797	16.522655	1
GTGTT	246190	1.4463751	16.129425	5
TTGAG	256045	1.3860033	9.217945	9
TGAGG	205830	1.3046113	5.2696238	10
TTGTG	209460	1.2305851	15.975301	3
>>END_MODULE