FastQCFastQC Report
Tue 12 Aug 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane2_Undetermined_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5345021
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 24815 0.4642638448006098 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2094885 5.192148 5.537074 43
TTTTT 1565020 3.584766 4.344468 16
TTTGA 873335 2.5206444 17.88635 8
TGTTT 876280 2.4895706 17.663185 6
GTTTG 664235 2.3406901 21.039658 7
CTTTG 605550 2.2393906 22.330881 1
TGTGT 629295 2.2175655 20.672234 4
GTGTT 550070 1.9383855 20.712917 5
GAGGG 343270 1.8905516 6.07434 11
TTGAG 495205 1.7727865 12.769413 9
TTTGT 618395 1.7569021 17.025501 2
TGAGG 372065 1.6520791 7.6340117 10
TTGTG 463540 1.6334634 20.561598 3
ACTTT 434900 1.3172791 8.121706 3
AACTT 395830 1.2179971 8.375093 2
TAACT 262555 0.8079005 8.0021305 1
Produced by FastQC (version 0.10.1)