##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane2_Undetermined_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5345021	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97345716695968	34.0	31.0	34.0	26.0	34.0
2	30.911597728053827	34.0	31.0	34.0	26.0	34.0
3	30.97071349205176	34.0	31.0	34.0	26.0	34.0
4	34.12333459494359	37.0	35.0	37.0	28.0	37.0
5	34.15533521757913	37.0	37.0	37.0	29.0	37.0
6	34.17227041764663	37.0	36.0	37.0	30.0	37.0
7	34.18921235295427	37.0	37.0	37.0	30.0	37.0
8	34.19683290299514	37.0	37.0	37.0	30.0	37.0
9	35.8507435237392	39.0	38.0	39.0	30.0	39.0
10	35.819304732385525	39.0	38.0	39.0	30.0	39.0
11	35.85298317817647	39.0	38.0	39.0	30.0	39.0
12	35.81025724688453	39.0	38.0	39.0	30.0	39.0
13	35.78978286521232	39.0	38.0	39.0	29.0	39.0
14	37.21301038854665	41.0	39.0	41.0	29.0	41.0
15	37.15110062242974	41.0	39.0	41.0	28.0	41.0
16	37.14249317261803	41.0	39.0	41.0	28.0	41.0
17	37.13743294928121	41.0	39.0	41.0	28.0	41.0
18	37.11975256972798	41.0	39.0	41.0	28.0	41.0
19	37.142531338978834	41.0	39.0	41.0	28.0	41.0
20	37.11729271035605	41.0	39.0	41.0	28.0	41.0
21	37.08648703157574	41.0	39.0	41.0	27.0	41.0
22	37.06809383910746	41.0	39.0	41.0	27.0	41.0
23	37.05322972538368	41.0	39.0	41.0	27.0	41.0
24	37.01111464295463	41.0	39.0	41.0	27.0	41.0
25	36.96556421387306	41.0	38.0	41.0	27.0	41.0
26	36.937532331491305	41.0	38.0	41.0	27.0	41.0
27	36.879572783717784	40.0	38.0	41.0	27.0	41.0
28	36.83701766559944	40.0	38.0	41.0	27.0	41.0
29	36.795811653499584	40.0	38.0	41.0	27.0	41.0
30	36.778661674107546	40.0	38.0	41.0	27.0	41.0
31	36.713011978811686	40.0	38.0	41.0	26.0	41.0
32	36.68075261070069	40.0	38.0	41.0	26.0	41.0
33	36.60007397538756	40.0	38.0	41.0	25.0	41.0
34	36.53170099799421	40.0	38.0	41.0	25.0	41.0
35	36.46455084086667	40.0	38.0	41.0	25.0	41.0
36	36.41633531467884	40.0	38.0	41.0	25.0	41.0
37	36.362888751980584	40.0	38.0	41.0	24.0	41.0
38	36.30966089749694	40.0	38.0	41.0	24.0	41.0
39	36.25660909470702	40.0	38.0	41.0	24.0	41.0
40	36.21135146148163	40.0	38.0	41.0	24.0	41.0
41	36.131013517065696	40.0	37.0	41.0	24.0	41.0
42	36.06600142450329	40.0	37.0	41.0	23.0	41.0
43	36.00928808324607	40.0	37.0	41.0	23.0	41.0
44	35.94454858081942	40.0	37.0	41.0	23.0	41.0
45	35.86260446871958	40.0	37.0	41.0	22.0	41.0
46	35.806139582987605	40.0	36.0	41.0	23.0	41.0
47	35.72877580836446	40.0	36.0	41.0	22.0	41.0
48	35.883613179443074	40.0	37.0	41.0	23.0	41.0
49	35.93411251330912	40.0	36.0	41.0	23.0	41.0
50	35.87591966430067	40.0	36.0	41.0	23.0	41.0
51	34.485200338782576	38.0	34.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26421.0
3	577.0
4	487.0
5	428.0
6	4011.0
7	19162.0
8	72469.0
9	92379.0
10	79707.0
11	47649.0
12	26083.0
13	18033.0
14	15116.0
15	13533.0
16	12060.0
17	11456.0
18	10966.0
19	10686.0
20	10456.0
21	10423.0
22	10449.0
23	11055.0
24	11269.0
25	12247.0
26	14102.0
27	15855.0
28	17931.0
29	20717.0
30	24591.0
31	30995.0
32	39511.0
33	53336.0
34	77348.0
35	121120.0
36	200981.0
37	370073.0
38	964548.0
39	2865662.0
40	1129.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.36601618025701	27.45003494244585	30.519049007298882	22.664899869998262
2	22.13483021317139	29.634641560094146	29.431761876722696	18.79876635001177
3	22.19631939930369	28.498757799616758	28.835981573478904	20.46894122760065
4	21.885069140411723	26.51002064673537	29.86868044144203	21.736229771410876
5	24.92093986463174	26.95643029332298	28.31934989523239	19.803279946812893
6	22.02447753579814	26.52687386029272	30.52359473880834	20.925053865100796
7	24.11021772973282	26.990902407991374	28.83963377884522	20.059246083430583
8	22.6143236022393	27.047767594116024	29.920025334932816	20.417883468711867
9	22.44366605961679	26.90123169304468	30.028786277688262	20.626315969650264
10	22.471086572803877	27.821491341966905	29.132747725878506	20.574674359350713
11	24.29741689322445	27.009807877870823	28.01979372847419	20.67298150043054
12	22.175946746023996	28.921978268143594	28.03447469685676	20.867600288975645
13	23.100081590606425	27.19924712239683	28.560251481300615	21.14041980569613
14	23.476736309671292	27.54175612669707	27.845092754232137	21.136414809399497
15	22.92219367133716	28.13179428950019	27.55320969192398	21.392802347238668
16	23.17711922681908	27.511611459166296	27.835844239296463	21.475425074718157
17	23.135573481835696	27.56629767206264	27.84070694401312	21.45742190208854
18	22.90216152898703	27.454933199641516	27.88827561120184	21.75462966016961
19	22.725509607206135	27.556513392819298	28.134499943955216	21.58347705601935
20	22.693835819121542	27.66377976056492	28.096730912712264	21.545653507601273
21	22.576000428022837	27.577424658938142	28.050340911146076	21.796234001892948
22	22.47703961152892	27.683082168741418	28.177499224002776	21.662378995726886
23	22.71160770278033	27.730005428467063	27.95850514387695	21.599881724875658
24	22.44795204057827	27.776278742484923	27.958736338981737	21.81703287795507
25	22.57183283197573	27.82596771547969	27.84073381297129	21.76146563957329
26	22.56685089755868	27.822484198228654	27.840192796006928	21.770472108205745
27	22.595955986590994	27.953251473081643	27.636532801270562	21.814259739056798
28	22.56972102178114	27.886300161624188	27.68333331764471	21.86064549894996
29	22.621184716383645	27.85884312048597	27.622406721820724	21.89756544130966
30	22.48547195004984	27.852011359148438	27.63011302728828	22.032403663513435
31	22.460644645913806	27.84206133701856	27.761556829556692	21.935737187510938
32	22.718092776162788	27.841523304306165	27.57058260247962	21.869801317051426
33	22.624679455925026	27.79871545173983	27.52309743372724	22.053507658607902
34	22.53409050624939	27.783093190648124	27.65586898263626	22.02694732046622
35	22.743880984475947	27.874354893938953	27.444167845017958	21.937596276567145
36	22.63192340267493	27.75726441144189	27.503121353074476	22.107690832808704
37	22.64627373669057	27.681390230730475	27.526000664822703	22.14633536775625
38	22.754793147767934	27.766799258322305	27.614865945136007	21.863541648773747
39	22.578334000846315	27.69945457709435	27.50405131935419	22.218160102705145
40	22.4941331130006	27.842079202880214	27.562546502362405	22.10124118175678
41	22.6165926620486	27.841590482111496	27.51314662502326	22.028670230816648
42	22.480173119707278	27.74832631947336	27.557674164518843	22.21382639630052
43	22.538073134220248	27.876196670449332	27.56857842190405	22.01715177342637
44	22.663407614778254	27.795867560417147	27.547837471756058	21.992887353048534
45	22.71212476291595	27.663140579069083	27.483231742926662	22.141502915088303
46	22.512487091820326	27.757376526584775	27.56574858210205	22.16438779949285
47	22.649138944422003	27.826880743529024	27.454573156819105	22.06940715522986
48	22.609717012192412	27.63683595506972	27.362011329758662	22.391435702979205
49	22.43791491389819	27.815654559732923	27.56347801469276	22.18295251167613
50	22.46224490353543	27.743087269518334	27.660607673184067	22.13406015376217
51	22.68110512795774	27.267677102679755	27.628820792831473	22.42239697653103
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.03091605025527
2	40.93359656318316
3	42.66526062690434
4	43.6212989118226
5	44.724219811444634
6	42.94953140089894
7	44.169463813163404
8	43.03220707095116
9	43.06998202926705
10	43.04576093215459
11	44.97039839365499
12	43.04354703499965
13	44.240501396302555
14	44.613151119070785
15	44.31499601857583
16	44.65254430153724
17	44.59299538392424
18	44.65679118915664
19	44.30898666322548
20	44.23948932672281
21	44.37223442991578
22	44.139418607255806
23	44.311489427655985
24	44.26498491853334
25	44.33329847154902
26	44.337323005764425
27	44.410215725647795
28	44.4303665207311
29	44.518750157693304
30	44.51787561356328
31	44.39638183342475
32	44.58789409321422
33	44.678187114532925
34	44.56103782671561
35	44.68147726104309
36	44.739614235483636
37	44.79260910444682
38	44.61833479654168
39	44.79649410355146
40	44.59537429475738
41	44.64526289286525
42	44.6939995160078
43	44.55522490764662
44	44.656294967826796
45	44.85362767800425
46	44.676874891313176
47	44.71854609965187
48	45.001152715171614
49	44.62086742557432
50	44.5963050572976
51	45.103502104488776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28046.0
1	15356.0
2	2666.0
3	2730.0
4	2794.0
5	4654.0
6	6514.0
7	7589.0
8	8664.0
9	7219.5
10	5775.0
11	5783.5
12	5792.0
13	6623.5
14	7455.0
15	9156.5
16	10858.0
17	13730.0
18	16602.0
19	19896.0
20	23190.0
21	27559.5
22	31929.0
23	37621.0
24	43313.0
25	52502.5
26	74418.0
27	87144.0
28	94510.5
29	101877.0
30	119545.0
31	137213.0
32	151953.0
33	166693.0
34	201524.5
35	236356.0
36	268089.5
37	299823.0
38	343209.0
39	386595.0
40	412264.0
41	437933.0
42	476988.0
43	516043.0
44	532947.0
45	549851.0
46	530851.0
47	511851.0
48	450377.5
49	388904.0
50	351208.5
51	313513.0
52	274892.5
53	236272.0
54	205546.5
55	174821.0
56	157080.5
57	139340.0
58	119617.0
59	99894.0
60	95427.0
61	90960.0
62	74028.0
63	57096.0
64	49161.5
65	41227.0
66	34397.0
67	27567.0
68	24383.5
69	21200.0
70	18867.5
71	16535.0
72	14264.0
73	11993.0
74	10244.5
75	7189.5
76	5883.0
77	5471.0
78	5059.0
79	5059.0
80	5059.0
81	4410.0
82	3761.0
83	3491.0
84	3221.0
85	2868.0
86	2515.0
87	2819.5
88	3124.0
89	2089.0
90	1054.0
91	704.0
92	354.0
93	273.5
94	193.0
95	157.5
96	122.0
97	99.5
98	77.0
99	94.5
100	112.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7860586515936981
2	0.5806151182567851
3	0.5585759157915376
4	0.5593055668069404
5	0.5509239346300042
6	0.5418687784388498
7	0.5404843124096238
8	0.5137865688460345
9	0.5350587022950892
10	0.5777526411963583
11	0.5537864116904312
12	0.5296518011809496
13	0.6199227280865688
14	0.7056286588958209
15	0.8260772034384898
16	0.7750016323602844
17	0.7174714561458224
18	0.7350017895158878
19	0.6878551085206214
20	0.6755445862607462
21	0.6902311515707796
22	0.6080799308365674
23	0.535133538296669
24	0.6398103955063974
25	0.5386134123701292
26	0.5840575743294554
27	0.6242632161781965
28	0.6233090571580542
29	0.6375840244593988
30	0.5186321999483258
31	0.6038329877469144
32	0.5720089780751095
33	0.6166299440170582
34	0.7817555815028604
35	0.8332240415893595
36	0.861306251182175
37	0.8862079307078494
38	0.8849731366817829
39	0.8745896414625873
40	0.776928659400964
41	0.8737664454452096
42	0.8870872537264118
43	0.8165543222374618
44	0.7713533772832698
45	0.6880609075249657
46	0.6985940747473209
47	0.7835142275399853
48	0.6701002671458166
49	0.6075373698251139
50	0.7156941011083026
51	0.7159747361142267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5345021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.74046760908628
#Duplication Level	Relative count
1	100.0
2	3.5553573838201906
3	1.0243653452392827
4	0.5573580057143863
5	0.4089247684030813
6	0.34321519299254316
7	0.25873145317899404
8	0.2252899728361309
9	0.1924351851308618
10++	10.772850211502696
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	24815	0.4642638448006098	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2094885	5.192148	5.537074	43
TTTTT	1565020	3.584766	4.344468	16
TTTGA	873335	2.5206444	17.88635	8
TGTTT	876280	2.4895706	17.663185	6
GTTTG	664235	2.3406901	21.039658	7
CTTTG	605550	2.2393906	22.330881	1
TGTGT	629295	2.2175655	20.672234	4
GTGTT	550070	1.9383855	20.712917	5
GAGGG	343270	1.8905516	6.07434	11
TTGAG	495205	1.7727865	12.769413	9
TTTGT	618395	1.7569021	17.025501	2
TGAGG	372065	1.6520791	7.6340117	10
TTGTG	463540	1.6334634	20.561598	3
ACTTT	434900	1.3172791	8.121706	3
AACTT	395830	1.2179971	8.375093	2
TAACT	262555	0.8079005	8.0021305	1
>>END_MODULE