FastQCFastQC Report
Tue 12 Aug 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane2_Undetermined_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5345021
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1276045 3.59604 4.443601 16
TGTTT 684375 2.2821295 23.234575 6
TGTGT 551280 2.1752338 26.714806 4
GTTTG 539430 2.1284766 26.799728 7
TTTGA 612540 1.9424441 21.798687 8
CTTTG 449860 1.835021 27.356136 1
TTTGT 542915 1.8104143 22.757082 2
GTGTT 448395 1.7692715 26.518196 5
GAGGG 298970 1.5707407 6.1694617 11
TTGAG 413280 1.5507653 14.9029 9
TTGTG 392655 1.5493335 26.48059 3
TGAGG 323800 1.4376929 8.305667 10
TTGAC 365210 1.4166881 5.052034 9
TTGAT 338015 1.0718896 5.533931 9