##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane2_Undetermined_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5345021	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04641497198982	34.0	33.0	34.0	31.0	34.0
2	33.23793657686284	34.0	34.0	34.0	31.0	34.0
3	33.285386343664506	34.0	34.0	34.0	31.0	34.0
4	36.580355437331306	37.0	37.0	37.0	35.0	37.0
5	36.51983967883382	37.0	37.0	37.0	35.0	37.0
6	36.51734371109112	37.0	37.0	37.0	35.0	37.0
7	36.51497365492109	37.0	37.0	37.0	35.0	37.0
8	36.49394211921712	37.0	37.0	37.0	35.0	37.0
9	38.30834715148921	39.0	39.0	39.0	37.0	39.0
10	38.295847668325344	39.0	39.0	39.0	37.0	39.0
11	38.29593260718714	39.0	39.0	39.0	37.0	39.0
12	38.25659693385676	39.0	39.0	39.0	37.0	39.0
13	38.2254524350793	39.0	39.0	39.0	37.0	39.0
14	39.8131266462751	41.0	40.0	41.0	38.0	41.0
15	39.788470615924616	41.0	40.0	41.0	38.0	41.0
16	39.768689028537025	41.0	40.0	41.0	38.0	41.0
17	39.73311498682606	41.0	40.0	41.0	38.0	41.0
18	39.73154941767301	41.0	40.0	41.0	38.0	41.0
19	39.713773435127756	41.0	40.0	41.0	38.0	41.0
20	39.64806667738069	41.0	40.0	41.0	38.0	41.0
21	39.63851330050902	41.0	40.0	41.0	37.0	41.0
22	39.58571126287436	41.0	40.0	41.0	37.0	41.0
23	39.56546569227698	41.0	40.0	41.0	37.0	41.0
24	39.55515366543929	41.0	40.0	41.0	37.0	41.0
25	39.52173433930381	41.0	40.0	41.0	37.0	41.0
26	39.41415365814279	41.0	39.0	41.0	37.0	41.0
27	39.317028127672465	41.0	39.0	41.0	37.0	41.0
28	39.24626863018873	41.0	39.0	41.0	36.0	41.0
29	39.21646201202951	40.0	39.0	41.0	36.0	41.0
30	39.13904472966524	40.0	39.0	41.0	36.0	41.0
31	39.090850157557846	40.0	39.0	41.0	36.0	41.0
32	38.987774416601916	40.0	39.0	41.0	36.0	41.0
33	38.88482402594863	40.0	39.0	41.0	35.0	41.0
34	38.819697808483824	40.0	38.0	41.0	35.0	41.0
35	38.7549927306179	40.0	38.0	41.0	35.0	41.0
36	38.66021705059718	40.0	38.0	41.0	35.0	41.0
37	38.82553239734699	40.0	39.0	41.0	35.0	41.0
38	38.867030644033015	40.0	39.0	41.0	35.0	41.0
39	38.81117454917389	40.0	39.0	41.0	35.0	41.0
40	38.78048411783602	40.0	39.0	41.0	35.0	41.0
41	38.720017376919564	40.0	38.0	41.0	35.0	41.0
42	38.64281038371973	40.0	38.0	41.0	35.0	41.0
43	38.59972823306026	40.0	38.0	41.0	35.0	41.0
44	38.53801322763746	40.0	38.0	41.0	35.0	41.0
45	38.45285808979983	40.0	38.0	41.0	35.0	41.0
46	38.38374648107089	40.0	38.0	41.0	35.0	41.0
47	38.27018210031354	40.0	38.0	41.0	34.0	41.0
48	38.22053795485556	40.0	38.0	41.0	34.0	41.0
49	38.17135423789729	40.0	38.0	41.0	34.0	41.0
50	38.233015361398955	40.0	38.0	41.0	34.0	41.0
51	37.951634614719005	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	15.0
7	4.0
8	1.0
9	6.0
10	3.0
11	7.0
12	11.0
13	19.0
14	54.0
15	77.0
16	187.0
17	364.0
18	782.0
19	1230.0
20	2448.0
21	3428.0
22	5186.0
23	6525.0
24	9096.0
25	11231.0
26	13971.0
27	16535.0
28	19299.0
29	22878.0
30	27343.0
31	33178.0
32	42092.0
33	55487.0
34	81438.0
35	129038.0
36	220861.0
37	431518.0
38	1127417.0
39	3081374.0
40	1918.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.37108788206925	28.97250560461377	26.92054369910885	23.73586281420813
2	22.668230158057455	28.697663688062136	29.089939860966023	19.544166292914387
3	21.232186913530864	27.319370546436055	30.076237362762363	21.37220517727072
4	21.928777670117068	27.290798601529172	29.382162443608312	21.398261284745445
5	24.811183949461714	27.7324468752859	27.169324250979933	20.287044924272458
6	22.54623508495102	27.09117513289471	28.82722069754263	21.53536908461164
7	24.825159714059122	27.958281174199318	26.35636043338277	20.860198678358792
8	22.266947127055253	27.767786132177964	28.76531261523575	21.199954125531033
9	21.809025633388533	27.60868479281934	29.19169073423659	21.39059883955554
10	22.05688247062079	27.989824548865194	28.580430273332887	21.37286270718113
11	24.492741638553206	27.7176011710366	26.427481493011797	21.362175697398396
12	22.111234091722082	30.062304771398253	26.374196073500993	21.45226506337867
13	23.38948715075207	28.260637329582057	26.722458153111088	21.62741736655478
14	22.969825562893018	28.364958715784276	26.92898680847091	21.736228912851793
15	23.00160730165091	28.004504410958837	26.97941228250866	22.014476004881587
16	22.936462782382595	28.25658478778988	26.84161991121063	21.9653325186169
17	23.022548173820613	28.383152402749424	26.687987843560702	21.906311579869268
18	22.735906930954997	28.03124627573961	26.944908167807014	22.287938625498384
19	22.610288235940214	28.446596836988387	26.96733009164654	21.97578483542486
20	22.633544212145555	28.505010538951463	26.922128505235047	21.93931674366793
21	22.608929606483482	28.230431100838167	26.893220340885748	22.26741895179261
22	22.584363676777823	28.535520888333732	26.85702246991295	22.023092964975497
23	22.72014833566745	28.43434584400993	26.889694057685887	21.95581176263674
24	22.473513200415866	28.28093659501057	26.941783764741057	22.303766439832508
25	22.581587251221418	28.62117854134713	26.695533444814178	22.101700762617273
26	22.746453999938982	28.4107327392496	26.66763873335094	22.175174527460477
27	22.693661272690626	28.29048273051024	26.651909845148907	22.363946151650225
28	22.65897283960612	28.581398164170707	26.477083241976633	22.28254575424654
29	22.833280718290823	28.597249263108544	26.470421920213127	22.09904809838751
30	22.660018832077895	28.35514026040993	26.627262475315426	22.35757843219675
31	22.63924401446872	28.541744703816374	26.436618297205527	22.38239298450938
32	22.88587798921549	28.638910078475398	26.409267856573887	22.065944075735228
33	22.785414525321823	28.38528934741647	26.447711521145788	22.381584606115915
34	22.66008698022083	28.55981792710261	26.454590678998297	22.32550441367826
35	22.956144745036152	28.378995549313714	26.49675386068784	22.168105844962295
36	22.93626541484762	28.270888679985624	26.382206443606997	22.410639461559757
37	22.898743696446754	28.47285697943293	26.31392398416446	22.314475339955855
38	22.910418683013518	28.49702184516965	26.49832241814451	22.094237053672323
39	22.921332952507598	28.196599725618178	26.48767857059245	22.394388751281774
40	22.772471111074466	28.638479265522516	26.205880512113428	22.38316911128959
41	22.77964727300823	28.5921563214735	26.487436416950278	22.14075998856799
42	22.808203910419557	28.318531600005702	26.48269386793196	22.390570621642777
43	22.861547877730516	28.601733848677526	26.209666228879126	22.327052044712833
44	22.90299106422232	28.495089797842965	26.338128868602983	22.263790269331732
45	22.915133240907416	28.31107916480624	26.367303202833764	22.406484391452583
46	22.872553808575983	28.473768418748964	26.253334959708795	22.400342812966258
47	22.879342769474416	28.526405298156266	26.285303045300072	22.308948887069246
48	22.769567791903107	28.34832154613487	26.308854687307466	22.573255974654558
49	22.699295321975672	28.677134821007737	26.21006915001746	22.413500706999123
50	22.784595828927536	28.50454078456958	26.39828792548764	22.312575461015246
51	22.8751411502273	28.246717484495015	26.38789918258377	22.490242182693915
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.10695069627738
2	42.21239645097184
3	42.60439209080158
4	43.32703895486251
5	45.09822887373417
6	44.08160416956267
7	45.68535839241791
8	43.46690125258629
9	43.19962447294407
10	43.42974517780192
11	45.8549173359516
12	43.56349915510076
13	45.016904517306855
14	44.706054475744814
15	45.0160833065325
16	44.901795300999495
17	44.928859753689885
18	45.02384555645338
19	44.58607307136507
20	44.57286095581349
21	44.87634855827609
22	44.607456641753316
23	44.67596009830419
24	44.77727964024837
25	44.68328801383869
26	44.92162852739946
27	45.05760742434085
28	44.941518593852656
29	44.93232881667833
30	45.01759726427464
31	45.021636998978096
32	44.95182206495071
33	45.166999131437734
34	44.985591393899085
35	45.12425058999845
36	45.34690487640738
37	45.213219036402606
38	45.00465573668584
39	45.31572170378937
40	45.155640222364056
41	44.92040726157622
42	45.19877453206234
43	45.18859992244335
44	45.16678133355405
45	45.32161763236
46	45.272896621542245
47	45.18829165654366
48	45.342823766557665
49	45.11279602897479
50	45.097171289942786
51	45.36538333292122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3410.0
1	2050.0
2	690.0
3	857.5
4	1025.0
5	1937.0
6	2849.0
7	4506.5
8	6164.0
9	4578.5
10	2993.0
11	2955.0
12	2917.0
13	3631.5
14	4346.0
15	6054.5
16	7763.0
17	10462.0
18	13161.0
19	16695.0
20	20229.0
21	24242.0
22	28255.0
23	33705.0
24	39155.0
25	48460.0
26	72373.0
27	86981.0
28	90467.0
29	93953.0
30	107220.0
31	120487.0
32	139971.0
33	159455.0
34	188604.0
35	217753.0
36	258039.0
37	298325.0
38	326926.5
39	355528.0
40	392704.5
41	429881.0
42	475497.0
43	521113.0
44	536184.5
45	551256.0
46	527012.0
47	502768.0
48	471723.0
49	440678.0
50	401432.0
51	362186.0
52	317098.5
53	272011.0
54	236248.5
55	200486.0
56	174230.5
57	147975.0
58	123416.5
59	98858.0
60	89502.5
61	80147.0
62	66485.0
63	52823.0
64	45708.0
65	38593.0
66	32303.5
67	26014.0
68	24540.0
69	23066.0
70	20643.0
71	18220.0
72	15806.0
73	13392.0
74	11318.0
75	7716.5
76	6189.0
77	5780.0
78	5371.0
79	5584.5
80	5798.0
81	5112.5
82	4427.0
83	3956.0
84	3485.0
85	3140.0
86	2795.0
87	3217.0
88	3639.0
89	2329.0
90	1019.0
91	609.0
92	199.0
93	147.5
94	96.0
95	57.5
96	19.0
97	19.0
98	19.0
99	34.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2396061680580862
2	0.010701548225909682
3	7.483600157978799E-5
4	7.483600157978799E-4
5	7.483600157978799E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.4321700513431097E-4
12	9.354500197473499E-5
13	0.0
14	0.0
15	7.10942015007986E-4
16	2.24508004739364E-4
17	0.025275859533573395
18	0.0
19	0.012796956270143748
20	0.0016276830343603887
21	0.004471451094392333
22	0.00679136714336576
23	0.007221674152449542
24	0.0
25	1.30963002764629E-4
26	0.04587446896841004
27	0.12110335955649193
28	0.16454565847355884
29	0.08125318871525482
30	0.07734300763271089
31	0.05638892719037025
32	0.17943802278793666
33	0.16119674740286333
34	0.1873706389553942
35	0.16819391355057353
36	0.2520663623211209
37	0.2378101040201713
38	0.0804112836974822
39	0.10750191626936545
40	0.15990582637561201
41	0.23620112998620585
42	0.2893346911078553
43	0.22722080979663128
44	0.0919547369411645
45	0.23315156292182948
46	0.149672003159576
47	0.19900763720105122
48	0.0944243249932975
49	0.05094460807544067
50	0.20843697340010453
51	0.12604253566075793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5345021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.82473520312012
#Duplication Level	Relative count
1	100.0
2	4.392551837846557
3	1.1547399296283685
4	0.6374068830385252
5	0.42354403006027586
6	0.3237811908218188
7	0.30765186950781676
8	0.23656337927202997
9	0.2126680884364714
10++	11.787546969181049
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1276045	3.59604	4.443601	16
TGTTT	684375	2.2821295	23.234575	6
TGTGT	551280	2.1752338	26.714806	4
GTTTG	539430	2.1284766	26.799728	7
TTTGA	612540	1.9424441	21.798687	8
CTTTG	449860	1.835021	27.356136	1
TTTGT	542915	1.8104143	22.757082	2
GTGTT	448395	1.7692715	26.518196	5
GAGGG	298970	1.5707407	6.1694617	11
TTGAG	413280	1.5507653	14.9029	9
TTGTG	392655	1.5493335	26.48059	3
TGAGG	323800	1.4376929	8.305667	10
TTGAC	365210	1.4166881	5.052034	9
TTGAT	338015	1.0718896	5.533931	9
>>END_MODULE