FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_9_ATCACG_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_9_ATCACG_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25704617
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 107906 0.41979228867716645 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8292845 3.7568448 4.682109 16
TGTGT 5033935 3.515343 31.907333 4
AAAAA 5465925 3.0795248 3.220927 10
TGTTT 4774155 2.6852603 25.838547 6
GTTTG 3754450 2.6218412 31.34337 7
TTTGA 4165180 2.4471676 26.6018 8
GTGTT 3490300 2.4373775 31.294117 5
CTTTG 3262570 2.4074898 33.249577 1
TTGTG 3214565 2.244824 31.256123 3
GAGGG 1868530 2.10107 8.50193 11
TTTGT 3708950 2.0861275 25.491507 2
TTGAG 2571115 1.8755199 18.929596 9
TGAGG 2022385 1.8316149 11.023338 10
ACTTT 2407825 1.4948561 10.198251 3
AACTT 2273735 1.4745327 11.022344 2
TGAGT 1631145 1.1898514 5.602062 10
TTGAT 1918150 1.1269704 6.1239743 9
TAACT 1642825 1.0653832 10.774701 1
TTGAC 1304045 1.0051647 5.9436774 9
Produced by FastQC (version 0.10.1)