##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_9_ATCACG_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25704617	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29254596557498	34.0	31.0	34.0	31.0	34.0
2	32.47133579154282	34.0	31.0	34.0	31.0	34.0
3	32.54596382431996	34.0	31.0	34.0	31.0	34.0
4	35.918919118693736	37.0	37.0	37.0	35.0	37.0
5	35.9310286552801	37.0	37.0	37.0	35.0	37.0
6	35.920072763581736	37.0	37.0	37.0	35.0	37.0
7	35.940594991164424	37.0	37.0	37.0	35.0	37.0
8	35.948060576043595	37.0	37.0	37.0	35.0	37.0
9	37.71510779561508	39.0	39.0	39.0	35.0	39.0
10	37.69725621665555	39.0	38.0	39.0	35.0	39.0
11	37.699230297809926	39.0	39.0	39.0	35.0	39.0
12	37.66008795229277	39.0	38.0	39.0	35.0	39.0
13	37.654041100865264	39.0	38.0	39.0	35.0	39.0
14	39.16451332459068	41.0	40.0	41.0	36.0	41.0
15	39.12650991843216	41.0	40.0	41.0	36.0	41.0
16	39.104955658355074	41.0	40.0	41.0	36.0	41.0
17	39.066736182064105	41.0	40.0	41.0	36.0	41.0
18	39.03930519563859	41.0	39.0	41.0	36.0	41.0
19	39.04448123074543	41.0	40.0	41.0	36.0	41.0
20	39.007963822219175	41.0	40.0	41.0	36.0	41.0
21	38.97320201269679	41.0	39.0	41.0	36.0	41.0
22	38.931629169965845	41.0	39.0	41.0	36.0	41.0
23	38.8974428990714	41.0	39.0	41.0	36.0	41.0
24	38.8636190144362	41.0	39.0	41.0	36.0	41.0
25	38.80976083012636	41.0	39.0	41.0	35.0	41.0
26	38.78066080502192	41.0	39.0	41.0	35.0	41.0
27	38.72895293479767	41.0	39.0	41.0	35.0	41.0
28	38.68864784096958	41.0	39.0	41.0	35.0	41.0
29	38.636044333980934	41.0	39.0	41.0	35.0	41.0
30	38.5884993345748	41.0	39.0	41.0	35.0	41.0
31	38.538085239706156	40.0	39.0	41.0	35.0	41.0
32	38.4858049042318	40.0	39.0	41.0	35.0	41.0
33	38.43045694864856	40.0	38.0	41.0	34.0	41.0
34	38.382594652159185	40.0	38.0	41.0	34.0	41.0
35	38.328609525673926	40.0	38.0	41.0	34.0	41.0
36	38.27844441331298	40.0	38.0	41.0	34.0	41.0
37	38.22247330119721	40.0	38.0	41.0	34.0	41.0
38	38.17520910737554	40.0	38.0	41.0	34.0	41.0
39	38.12349855280863	40.0	38.0	41.0	34.0	41.0
40	38.05261074304278	40.0	38.0	41.0	33.0	41.0
41	37.975901294308336	40.0	38.0	41.0	33.0	41.0
42	37.887663916564094	40.0	38.0	41.0	33.0	41.0
43	37.84182934917879	40.0	38.0	41.0	33.0	41.0
44	37.776211331995334	40.0	38.0	41.0	33.0	41.0
45	37.712303241087	40.0	38.0	41.0	33.0	41.0
46	37.64711071166709	40.0	38.0	41.0	33.0	41.0
47	37.57710511695234	40.0	37.0	41.0	32.0	41.0
48	37.795432120229606	40.0	38.0	41.0	33.0	41.0
49	37.841078200075884	40.0	38.0	41.0	33.0	41.0
50	37.81422944368321	40.0	38.0	41.0	33.0	41.0
51	36.29068023071497	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115354.0
3	5135.0
4	2598.0
5	1301.0
6	2165.0
7	3678.0
8	7010.0
9	10327.0
10	11756.0
11	14379.0
12	19158.0
13	24203.0
14	29427.0
15	32461.0
16	34031.0
17	35237.0
18	36432.0
19	37602.0
20	39862.0
21	42875.0
22	46904.0
23	52310.0
24	59667.0
25	68480.0
26	79636.0
27	94398.0
28	112148.0
29	134852.0
30	165875.0
31	208197.0
32	269941.0
33	362466.0
34	524150.0
35	787651.0
36	1206597.0
37	1977906.0
38	4397037.0
39	1.4642118E7
40	9293.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.033244099625687	27.140459853683097	29.23423127965486	23.592064767036355
2	22.222209185279606	28.421021178239503	30.558095477900487	18.798674158580404
3	22.35057272231274	27.291859065324193	30.370085074619396	19.987483137743673
4	21.88700246090224	25.601407351564674	31.25494830689746	21.256641880635623
5	26.095182177118115	25.755985118012028	28.333986775815216	19.81484592905464
6	21.731911242775446	25.418525674056713	32.36867676990778	20.48088631326006
7	24.946515041995706	25.844586940682294	29.220299697165846	19.98859832015615
8	22.43884764790951	25.91386213765477	31.296708717668487	20.35058149676724
9	22.37389194181569	26.048625901547908	31.261181413887247	20.31630074274916
10	22.39254261710894	27.123707013988536	30.25695235919199	20.226798009710535
11	25.33185747737851	25.919714994473164	28.270183431888128	20.478244096260198
12	21.62343842911096	29.40154275732351	28.36729989872941	20.60771891483612
13	23.66514363489352	26.269818344938617	28.991859685615633	21.07317833455223
14	24.079384043956757	26.565542829082656	28.095632187070212	21.25944093989037
15	23.36404390034327	27.549002278964828	27.65084824790333	21.436105572788573
16	23.613056292512933	26.81294804286664	27.933462425674616	21.64053323894581
17	23.447516436005365	26.840917044812336	28.055974211554947	21.65559230762735
18	23.28288901239373	26.783031425248694	28.132374165975783	21.801705396381795
19	22.981493139597173	27.065045768603635	28.388757463547385	21.5647036282518
20	22.74950696691805	27.11685470706967	28.414322536719737	21.71931578929254
21	22.836472292541366	27.160729714896092	28.263087947495503	21.73971004506704
22	22.640893865675658	27.271970856458278	28.451689689738636	21.635445588127432
23	22.643852432250853	27.384851768612855	28.286789380384235	21.684506418752054
24	22.522046140995318	27.420580967126938	28.23040440163168	21.82696849024606
25	22.533472343946908	27.5987335086899	28.109626458913233	21.75816768844996
26	22.563236858615678	27.525772623344363	28.094034749483228	21.81695576855673
27	22.575549224913527	27.501486188614543	28.00227699739839	21.92068758907354
28	22.563303951848837	27.558802891081445	27.993449831269373	21.884443325800344
29	22.63282518911661	27.54177979346396	27.924745913549508	21.900649103869917
30	22.615167105672267	27.506933159686575	27.869741465789826	22.00815826885133
31	22.734133897281325	27.48721146539877	27.771164693263312	22.00748994405659
32	22.75871696361598	27.537923265649017	27.688209400872733	22.01515036986227
33	22.729053965847335	27.482901336285178	27.665591350834518	22.122453347032966
34	22.770808186612072	27.506674718832603	27.626655381118038	22.095861713437287
35	22.779133703970544	27.475489678402994	27.593095415222407	22.152281202404055
36	22.81174506842418	27.48359029570854	27.585280215853636	22.11938442001364
37	22.80771667489844	27.410809639260552	27.608258778964885	22.173214906876126
38	22.76609805397312	27.45945400803861	27.687660360096988	22.086787577891286
39	22.73439974111281	27.3762839980596	27.659768444744355	22.229547816083244
40	22.78566229060233	27.40515669695086	27.63606472606343	22.173116286383376
41	22.781974151305825	27.374943698026673	27.64277932009007	22.20030283057743
42	22.72499931997031	27.35982729007092	27.62754176996758	22.287631619991192
43	22.758143541934047	27.449030500413237	27.534470312080376	22.258355645572337
44	22.847113473487795	27.367341954168577	27.54231626885153	22.243228303492096
45	22.82038770034123	27.32712579176555	27.533131358959096	22.319355148934125
46	22.732363168005392	27.466821551388033	27.471468718910085	22.32934656169649
47	22.78981006268689	27.409656755926186	27.499781064656663	22.300752116730262
48	22.75609136742122	27.31889242716089	27.46344256429088	22.46157364112701
49	22.790588713784963	27.43357718239722	27.44249319376574	22.333340910052076
50	22.709951700677188	27.42077063321068	27.506977516274873	22.362300149837264
51	22.776072306190617	27.35276937468165	27.463158177104308	22.408000142023425
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.625308866662046
2	41.020883343860014
3	42.338055860056414
4	43.143644341537865
5	45.91002810617276
6	42.21279755603551
7	44.93511336215186
8	42.789429144676745
9	42.69019268456485
10	42.61934062681947
11	45.810101573638704
12	42.23115734394708
13	44.73832196944575
14	45.33882498384713
15	44.80014947313184
16	45.25358953145874
17	45.10310874363272
18	45.08459440877552
19	44.546196767848976
20	44.46882275621059
21	44.57618233760841
22	44.27633945380309
23	44.32835885100291
24	44.34901463124138
25	44.29164003239687
26	44.38019262717241
27	44.49623681398707
28	44.44774727764918
29	44.533474292986526
30	44.623325374523596
31	44.74162384133792
32	44.77386733347825
33	44.8515073128803
34	44.86666990004936
35	44.9314149063746
36	44.93112948843782
37	44.98093158177457
38	44.8528856318644
39	44.96394755719605
40	44.95877857698571
41	44.98227698188326
42	45.012630939961504
43	45.01649918750638
44	45.09034177697989
45	45.13974284927535
46	45.06170972970188
47	45.090562179417155
48	45.21766500854823
49	45.123929623837036
50	45.07225185051445
51	45.18407244821404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124459.0
1	66442.0
2	8425.0
3	8948.5
4	9472.0
5	18044.5
6	26617.0
7	32307.0
8	37997.0
9	29840.5
10	21684.0
11	22238.0
12	22792.0
13	28659.0
14	34526.0
15	47969.0
16	61412.0
17	89338.5
18	117265.0
19	149536.0
20	181807.0
21	217217.5
22	252628.0
23	300162.0
24	347696.0
25	413054.0
26	564403.0
27	650394.0
28	710898.5
29	771403.0
30	828303.0
31	885203.0
32	957918.0
33	1030633.0
34	1103802.0
35	1176971.0
36	1299501.0
37	1422031.0
38	1490230.5
39	1558430.0
40	1635240.0
41	1712050.0
42	1728427.5
43	1744805.0
44	1752417.0
45	1760029.0
46	1737853.5
47	1715678.0
48	1677696.0
49	1639714.0
50	1572883.5
51	1506053.0
52	1409165.0
53	1312277.0
54	1217854.0
55	1123431.0
56	1028113.0
57	932795.0
58	833336.0
59	733877.0
60	653656.0
61	573435.0
62	497533.0
63	421631.0
64	364209.0
65	306787.0
66	259209.0
67	211631.0
68	198634.0
69	185637.0
70	166484.0
71	147331.0
72	127629.0
73	107927.0
74	89795.5
75	60492.0
76	49320.0
77	47261.5
78	45203.0
79	45497.5
80	45792.0
81	40439.5
82	35087.0
83	31515.0
84	27943.0
85	25357.0
86	22771.0
87	28311.5
88	33852.0
89	22662.5
90	11473.0
91	7216.0
92	2959.0
93	2295.0
94	1631.0
95	1262.5
96	894.0
97	716.0
98	538.0
99	341.5
100	145.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.89018249133998
2	0.5300604167725976
3	0.5163002428707651
4	0.5114878778392224
5	0.504998771232421
6	0.49978569997755656
7	0.48991198740677594
8	0.44390857875843864
9	0.4571668972931983
10	0.4613568060555036
11	0.47881670440761676
12	0.46950320247914995
13	0.4562448839443903
14	0.4463750617252924
15	0.4932032249303695
16	0.46417342067380346
17	0.48788511418007124
18	0.49629994486982626
19	0.506978960238933
20	0.5304611229959194
21	0.5162963525190825
22	0.5504536402934928
23	0.5191557610058924
24	0.5457346437023357
25	0.5414708182580585
26	0.5268742187444381
27	0.528531508561283
28	0.5212215377494245
29	0.5404048618969892
30	0.5270142714050164
31	0.5412996427840181
32	0.565552095174186
33	0.5254892535453846
34	0.5368568611623351
35	0.5362344048930976
36	0.5470923764396101
37	0.5409378400775238
38	0.55330915842862
39	0.5319044434702139
40	0.5408639233955519
41	0.559393668460417
42	0.6001334312820145
43	0.5235246259456035
44	0.5613544057085154
45	0.566431314654484
46	0.5472090869900921
47	0.5357325495260248
48	0.5411206866066123
49	0.5596659930782084
50	0.5376971771258059
51	0.5110793909125353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5704617E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.82495866125724
#Duplication Level	Relative count
1	100.0
2	11.799867461895294
3	4.124585818422797
4	2.1139827700463885
5	1.2736911862160372
6	0.9158383035122598
7	0.6527501656726309
8	0.5414181577203446
9	0.4479787939032472
10++	10.667992047713717
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	107906	0.41979228867716645	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8292845	3.7568448	4.682109	16
TGTGT	5033935	3.515343	31.907333	4
AAAAA	5465925	3.0795248	3.220927	10
TGTTT	4774155	2.6852603	25.838547	6
GTTTG	3754450	2.6218412	31.34337	7
TTTGA	4165180	2.4471676	26.6018	8
GTGTT	3490300	2.4373775	31.294117	5
CTTTG	3262570	2.4074898	33.249577	1
TTGTG	3214565	2.244824	31.256123	3
GAGGG	1868530	2.10107	8.50193	11
TTTGT	3708950	2.0861275	25.491507	2
TTGAG	2571115	1.8755199	18.929596	9
TGAGG	2022385	1.8316149	11.023338	10
ACTTT	2407825	1.4948561	10.198251	3
AACTT	2273735	1.4745327	11.022344	2
TGAGT	1631145	1.1898514	5.602062	10
TTGAT	1918150	1.1269704	6.1239743	9
TAACT	1642825	1.0653832	10.774701	1
TTGAC	1304045	1.0051647	5.9436774	9
>>END_MODULE