FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_9_ATCACG_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_9_ATCACG_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25704617
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 7740470 3.5868351 4.3406186 16
TGTGT 4167270 3.0000243 32.982574 4
TGTTT 4048600 2.3383715 26.570139 6
CTTTG 2920580 2.1817725 33.507507 1
GTTTG 2996210 2.1569762 32.26548 7
TTTGA 3446320 2.053298 26.98084 8
GAGGG 1717955 1.9819912 8.635853 11
GTGTT 2732685 1.9672643 32.225567 5
TTTGT 3360365 1.9408638 26.190495 2
TTGTG 2512205 1.8085403 32.184837 3
TGAGG 1845845 1.7085221 11.423341 10
TTGAG 2156785 1.6016505 19.212976 9
TGAGT 1533730 1.1389635 5.61615 10
TTGAT 1770150 1.0546453 6.3229523 9
TTGAC 1203710 0.92757684 5.9031343 9
Produced by FastQC (version 0.10.1)