##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_9_ATCACG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25704617	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94105677590917	34.0	33.0	34.0	31.0	34.0
2	33.15613393500475	34.0	34.0	34.0	31.0	34.0
3	33.19663677540887	34.0	34.0	34.0	31.0	34.0
4	36.525584644968646	37.0	37.0	37.0	35.0	37.0
5	36.45590953562934	37.0	37.0	37.0	35.0	37.0
6	36.4153012277911	37.0	37.0	37.0	35.0	37.0
7	36.42876165009578	37.0	37.0	37.0	35.0	37.0
8	36.41249395001684	37.0	37.0	37.0	35.0	37.0
9	38.233772672045646	39.0	39.0	39.0	37.0	39.0
10	38.2079962521908	39.0	39.0	39.0	37.0	39.0
11	38.18060549200169	39.0	39.0	39.0	37.0	39.0
12	38.14435628432044	39.0	39.0	39.0	37.0	39.0
13	38.14151656101314	39.0	39.0	39.0	37.0	39.0
14	39.69920489381344	41.0	40.0	41.0	37.0	41.0
15	39.676801292156966	41.0	40.0	41.0	37.0	41.0
16	39.65809002328259	41.0	40.0	41.0	37.0	41.0
17	39.63849261010191	41.0	40.0	41.0	37.0	41.0
18	39.60037233777885	41.0	40.0	41.0	37.0	41.0
19	39.598581686706325	41.0	40.0	41.0	37.0	41.0
20	39.57166270946578	41.0	40.0	41.0	37.0	41.0
21	39.50744716406395	41.0	40.0	41.0	37.0	41.0
22	39.469661306371535	41.0	39.0	41.0	37.0	41.0
23	39.42929750713656	41.0	39.0	41.0	37.0	41.0
24	39.41443780313863	41.0	39.0	41.0	36.0	41.0
25	39.34664854177753	41.0	39.0	41.0	36.0	41.0
26	39.26506864506092	41.0	39.0	41.0	36.0	41.0
27	39.21213045111701	41.0	39.0	41.0	36.0	41.0
28	39.156173966723564	40.0	39.0	41.0	36.0	41.0
29	39.09965450953811	40.0	39.0	41.0	36.0	41.0
30	39.03271859681862	40.0	39.0	41.0	36.0	41.0
31	38.956277076604565	40.0	39.0	41.0	35.0	41.0
32	38.88707367240679	40.0	38.0	41.0	35.0	41.0
33	38.81272605617893	40.0	38.0	41.0	35.0	41.0
34	38.74658116866709	40.0	38.0	41.0	35.0	41.0
35	38.67787642974801	40.0	38.0	41.0	35.0	41.0
36	38.59751487446788	40.0	38.0	41.0	35.0	41.0
37	38.79223627412927	40.0	38.0	41.0	35.0	41.0
38	38.81968974678751	40.0	38.0	41.0	35.0	41.0
39	38.799026532859834	40.0	38.0	41.0	35.0	41.0
40	38.76387385970388	40.0	38.0	41.0	35.0	41.0
41	38.707609064939575	40.0	38.0	41.0	35.0	41.0
42	38.64142768592895	40.0	38.0	41.0	35.0	41.0
43	38.59965347859492	40.0	38.0	41.0	35.0	41.0
44	38.54184121864177	40.0	38.0	41.0	35.0	41.0
45	38.47382211530326	40.0	38.0	41.0	34.0	41.0
46	38.416447831142555	40.0	38.0	41.0	34.0	41.0
47	38.336636994046636	40.0	38.0	41.0	34.0	41.0
48	38.27799033146458	40.0	38.0	41.0	34.0	41.0
49	38.220852230554534	40.0	38.0	41.0	34.0	41.0
50	38.30642312235191	40.0	38.0	41.0	34.0	41.0
51	38.04319605306704	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	18.0
12	54.0
13	116.0
14	178.0
15	311.0
16	498.0
17	823.0
18	1435.0
19	2524.0
20	4823.0
21	8220.0
22	13274.0
23	19937.0
24	29468.0
25	40951.0
26	55903.0
27	74258.0
28	97725.0
29	128439.0
30	166366.0
31	214568.0
32	280572.0
33	375028.0
34	540404.0
35	811106.0
36	1241354.0
37	2063093.0
38	4663719.0
39	1.4856521E7
40	12923.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.653520940398206	27.8005963093233	27.713373012651708	23.832509737626783
2	22.345302136032736	27.4095494074997	30.735687372211718	19.509461084255847
3	21.247485371289716	26.36428357736887	31.869031951102894	20.519199100238517
4	22.00377076227201	26.170660313670496	31.371227978226635	20.45434094583086
5	25.42218500278238	26.620145306242364	27.900596655665787	20.057073035309468
6	22.253750065746946	26.244535222778666	30.55735203358027	20.94436267789412
7	25.268934984896646	26.82948872055898	27.366952137087114	20.534624157457262
8	21.738125878319835	26.754796618833105	30.522715043760428	20.98436245908663
9	21.335268290517615	26.76587634042554	30.943662766887364	20.955192602169486
10	21.690706381658984	26.747580016461637	30.721298045405614	20.840415556473765
11	24.960698694713095	26.642338222740296	27.487307046823535	20.909656035723074
12	21.6108580807174	30.061139051122083	27.29584636121587	21.03215650694465
13	23.471853325027173	26.985926302656054	28.10654988557114	21.435670486745632
14	22.950049061368222	27.141401702469548	28.330938555528434	21.577610680633796
15	23.067112807010506	27.120166777820497	28.090934013916645	21.721786401252352
16	22.957253165841763	27.109977946763415	28.057317485026132	21.875451402368686
17	22.98726722907406	27.189695143094333	27.959794149043343	21.863243478788267
18	22.806299739848292	27.14517395843712	28.1169021113989	21.931624190315695
19	22.539718667584093	27.410618529405983	28.34504335890105	21.70461944410887
20	22.37261500531208	27.416277005800165	28.37364198034929	21.837466008538467
21	22.503626488579854	27.416576562879737	28.176109373658438	21.903687574881975
22	22.448445740311946	27.45016196895678	28.268618046322185	21.83277424440909
23	22.522533597757942	27.49874079041909	28.108405583323805	21.87032002849916
24	22.356415627890534	27.51787539417057	28.12535013177404	22.000358846164858
25	22.43572039995772	27.673289199368345	28.000950179494993	21.890040221178943
26	22.458378716370838	27.602138484520122	27.950177556027917	21.98930524308112
27	22.462645523953924	27.554314464206957	27.885612923156955	22.09742708868216
28	22.439669106915698	27.628219475123867	27.911643266266132	22.020468151694306
29	22.5221070530814	27.626719242400256	27.846104246216957	22.005069458301385
30	22.49594643623683	27.547641191194927	27.85406563555966	22.10234673700858
31	22.63044806308532	27.537936083622643	27.703330495062424	22.12828535822961
32	22.6484565010247	27.64562101820074	27.611319787414068	22.094602693360496
33	22.62061140269968	27.533110128122217	27.630936987652444	22.21534148152566
34	22.596633126258993	27.591860248297028	27.624364136606278	22.187142488837704
35	22.605443282313544	27.57645216901052	27.599627008409893	22.21847754026604
36	22.69830428662541	27.561584443977083	27.520965037120792	22.219146232276714
37	22.696837937667542	27.52286800195374	27.54750952018871	22.23278454019001
38	22.657170888794028	27.537169684341144	27.647737369516147	22.157922057348685
39	22.65068477641852	27.432638746290085	27.634701304020716	22.281975173270677
40	22.64547767627881	27.525751256633313	27.610151885339018	22.21861918174886
41	22.677502748922606	27.462195895087483	27.615374608202664	22.244926747787247
42	22.600245528152406	27.461848445110267	27.595104811639075	22.342801215098255
43	22.65137310338783	27.55828795199673	27.491486634893086	22.298852309722356
44	22.739763865376382	27.412931752359178	27.533533649193593	22.313770733070847
45	22.728849383592102	27.367853265003074	27.513282657519657	22.390014693885174
46	22.616963726509052	27.49489010282974	27.505335732858594	22.382810437802615
47	22.61835516326362	27.53621541897584	27.510809007829458	22.334620409931084
48	22.6539584280392	27.409662517406915	27.474047917910365	22.46233113664352
49	22.751769298177223	27.46176266403853	27.4437806155852	22.342687422199045
50	22.6060743946209	27.45658067716808	27.496449610772146	22.440895317438876
51	22.678462783553634	27.437172084688132	27.425551604211805	22.458813527546432
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.48603067802499
2	41.85476322028858
3	41.76668447152824
4	42.45811170810287
5	45.47925803809185
6	43.19811274364106
7	45.803559142353905
8	42.72248833740647
9	42.2904608926871
10	42.53112193813275
11	45.87035473043617
12	42.64301458766205
13	44.907523811772805
14	44.527659742002015
15	44.788899208262855
16	44.83270456821045
17	44.85051070786233
18	44.73792393016399
19	44.24433811169297
20	44.21008101385054
21	44.407314063461826
22	44.281219984721034
23	44.39285362625711
24	44.35677447405539
25	44.32576062113666
26	44.44768395945196
27	44.560072612636084
28	44.46013725861
29	44.527176511382784
30	44.59829317324541
31	44.758733421314936
32	44.7430591943852
33	44.835952884225335
34	44.783775615096694
35	44.82392082257958
36	44.91745051890212
37	44.92962247785755
38	44.81509294614271
39	44.932659949689196
40	44.86409685802767
41	44.92242949670985
42	44.94304674325066
43	44.95022541311018
44	45.05353459844723
45	45.11886407747727
46	44.99977416431167
47	44.952975573194706
48	45.116289564682724
49	45.09445672037627
50	45.04696971205978
51	45.13727631110007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20012.0
1	11983.5
2	3955.0
3	5355.5
4	6756.0
5	9963.0
6	13170.0
7	21466.0
8	29762.0
9	23656.5
10	17551.0
11	18942.5
12	20334.0
13	26953.5
14	33573.0
15	48459.0
16	63345.0
17	94304.5
18	125264.0
19	159613.5
20	193963.0
21	230888.5
22	267814.0
23	317613.5
24	367413.0
25	436226.0
26	594967.0
27	684895.0
28	739834.5
29	794774.0
30	846261.0
31	897748.0
32	968403.0
33	1039058.0
34	1107417.0
35	1175776.0
36	1295107.0
37	1414438.0
38	1478923.0
39	1543408.0
40	1614785.5
41	1686163.0
42	1700720.5
43	1715278.0
44	1724517.0
45	1733756.0
46	1711922.5
47	1690089.0
48	1659696.0
49	1629303.0
50	1564345.5
51	1499388.0
52	1411258.5
53	1323129.0
54	1231021.0
55	1138913.0
56	1044047.5
57	949182.0
58	848705.5
59	748229.0
60	668318.5
61	588408.0
62	510851.0
63	433294.0
64	374709.5
65	316125.0
66	267180.5
67	218236.0
68	206375.5
69	194515.0
70	174514.5
71	154514.0
72	134220.5
73	113927.0
74	94577.5
75	62160.5
76	49093.0
77	47701.5
78	46310.0
79	47462.5
80	48615.0
81	42518.5
82	36422.0
83	32201.0
84	27980.0
85	25636.0
86	23292.0
87	29257.5
88	35223.0
89	22560.5
90	9898.0
91	5615.5
92	1333.0
93	946.0
94	559.0
95	340.0
96	121.0
97	76.5
98	32.0
99	23.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2694613189529336
2	0.009476896699141637
3	1.0503949543383588E-4
4	0.0
5	3.501316514461196E-5
6	3.890351682734662E-6
7	0.0028321760250308342
8	0.0
9	0.0
10	0.0
11	0.0
12	0.006738089114496435
13	0.0
14	2.917763762050996E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.008877782540000498
20	0.0
21	0.0
22	0.0
23	0.0
24	3.501316514461196E-5
25	0.0
26	0.0045945053373096355
27	0.0
28	0.0
29	0.0019062723245399844
30	2.917763762050996E-4
31	0.0
32	0.0
33	7.780703365469324E-5
34	0.0
35	6.224562692375459E-5
36	6.030045108238727E-4
37	1.2449125384750919E-4
38	0.0
39	0.004633408854136983
40	0.003956487661341152
41	3.890351682734662E-6
42	1.128201987993052E-4
43	1.322719572129785E-4
44	8.247545567397483E-4
45	1.828465290885291E-4
46	3.4235094808065023E-4
47	0.00950412916092078
48	1.2449125384750919E-4
49	0.004687873777695268
50	0.0015289082113147223
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5704617E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.101407692997405
#Duplication Level	Relative count
1	100.0
2	12.552270412266124
3	4.338444218088243
4	2.2080898514984186
5	1.3878464590146358
6	0.9013295448453332
7	0.7398273736128237
8	0.5401275934166085
9	0.46976357690451936
10++	10.888999088618453
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	7740470	3.5868351	4.3406186	16
TGTGT	4167270	3.0000243	32.982574	4
TGTTT	4048600	2.3383715	26.570139	6
CTTTG	2920580	2.1817725	33.507507	1
GTTTG	2996210	2.1569762	32.26548	7
TTTGA	3446320	2.053298	26.98084	8
GAGGG	1717955	1.9819912	8.635853	11
GTGTT	2732685	1.9672643	32.225567	5
TTTGT	3360365	1.9408638	26.190495	2
TTGTG	2512205	1.8085403	32.184837	3
TGAGG	1845845	1.7085221	11.423341	10
TTGAG	2156785	1.6016505	19.212976	9
TGAGT	1533730	1.1389635	5.61615	10
TTGAT	1770150	1.0546453	6.3229523	9
TTGAC	1203710	0.92757684	5.9031343	9
>>END_MODULE