FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_5_GCCAAT_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_5_GCCAAT_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 22311992
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 101198 0.4535587857865851 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 3659185 3.0418158 19.85675 4
TGTTT 3602625 2.2702718 15.250514 6
GTTTG 2566135 2.1331825 19.155317 7
GAGGG 1370525 2.028136 6.782182 11
CTTTG 2330040 2.0181515 20.089933 1
TTTGA 2926315 1.8865225 15.1281185 8
GTGTT 2268580 1.8858304 19.068975 5
TTGTG 2177370 1.810009 19.075014 3
TTTGT 2847270 1.7942685 14.864543 2
TGAGG 1597290 1.7918605 7.9274297 10
TTGAG 1886665 1.6044472 12.076051 9
ACTTT 1918625 1.2887622 5.4572496 3
AACTT 1874425 1.2880505 5.7884164 2
TAACT 1311820 0.90144473 5.497137 1
Produced by FastQC (version 0.10.1)