##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_5_GCCAAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22311992	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44162538244008	34.0	31.0	34.0	31.0	34.0
2	32.597229552610095	34.0	31.0	34.0	31.0	34.0
3	32.66938778931079	34.0	31.0	34.0	31.0	34.0
4	36.03047701881571	37.0	37.0	37.0	35.0	37.0
5	36.04330456016657	37.0	37.0	37.0	35.0	37.0
6	36.03741131674841	37.0	37.0	37.0	35.0	37.0
7	36.05279734772225	37.0	37.0	37.0	35.0	37.0
8	36.050687495764606	37.0	37.0	37.0	35.0	37.0
9	37.849734752504396	39.0	39.0	39.0	37.0	39.0
10	37.82758415295237	39.0	39.0	39.0	37.0	39.0
11	37.82447277679196	39.0	39.0	39.0	37.0	39.0
12	37.79822110907892	39.0	39.0	39.0	37.0	39.0
13	37.77631329376597	39.0	39.0	39.0	35.0	39.0
14	39.325041663693675	41.0	40.0	41.0	37.0	41.0
15	39.30334288395227	41.0	40.0	41.0	37.0	41.0
16	39.291350274776	41.0	40.0	41.0	37.0	41.0
17	39.27004944247022	41.0	40.0	41.0	37.0	41.0
18	39.249420715102445	41.0	40.0	41.0	37.0	41.0
19	39.26491480455891	41.0	40.0	41.0	37.0	41.0
20	39.241134139883165	41.0	40.0	41.0	37.0	41.0
21	39.2079085991067	41.0	40.0	41.0	37.0	41.0
22	39.17624629840312	41.0	40.0	41.0	37.0	41.0
23	39.158708285660914	41.0	40.0	41.0	36.0	41.0
24	39.134803203586664	41.0	40.0	41.0	36.0	41.0
25	39.08337780866899	41.0	39.0	41.0	36.0	41.0
26	39.05138366847747	41.0	39.0	41.0	36.0	41.0
27	39.01598248152832	41.0	39.0	41.0	36.0	41.0
28	38.98619007213699	41.0	39.0	41.0	36.0	41.0
29	38.93510781108204	41.0	39.0	41.0	36.0	41.0
30	38.89576811429477	41.0	39.0	41.0	36.0	41.0
31	38.86053607405381	41.0	39.0	41.0	36.0	41.0
32	38.817360502818396	41.0	39.0	41.0	35.0	41.0
33	38.7751066332401	41.0	39.0	41.0	35.0	41.0
34	38.74008676589701	41.0	39.0	41.0	35.0	41.0
35	38.697466546241145	41.0	39.0	41.0	35.0	41.0
36	38.65922500330764	41.0	39.0	41.0	35.0	41.0
37	38.62457677467794	40.0	39.0	41.0	35.0	41.0
38	38.58920324101945	40.0	39.0	41.0	35.0	41.0
39	38.55686735635258	40.0	39.0	41.0	35.0	41.0
40	38.49951322141026	40.0	38.0	41.0	35.0	41.0
41	38.45517087851233	40.0	38.0	41.0	35.0	41.0
42	38.4008400504984	40.0	38.0	41.0	34.0	41.0
43	38.37640144367209	40.0	38.0	41.0	34.0	41.0
44	38.32249137593811	40.0	38.0	41.0	34.0	41.0
45	38.27660842653583	40.0	38.0	41.0	34.0	41.0
46	38.225720814170245	40.0	38.0	41.0	34.0	41.0
47	38.162209138475845	40.0	38.0	41.0	34.0	41.0
48	38.313841229416	40.0	38.0	41.0	34.0	41.0
49	38.34504763178474	40.0	38.0	41.0	34.0	41.0
50	38.317581684324736	40.0	38.0	41.0	34.0	41.0
51	36.8196946736087	39.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105982.0
3	2452.0
4	2023.0
5	1286.0
6	1581.0
7	2048.0
8	4707.0
9	7221.0
10	8043.0
11	9232.0
12	11766.0
13	14943.0
14	17658.0
15	18992.0
16	20101.0
17	20273.0
18	21808.0
19	22456.0
20	24395.0
21	26696.0
22	29788.0
23	34315.0
24	39702.0
25	46545.0
26	54504.0
27	65084.0
28	79094.0
29	96324.0
30	119496.0
31	151660.0
32	199667.0
33	271106.0
34	391163.0
35	589363.0
36	910060.0
37	1535429.0
38	3512184.0
39	1.3830881E7
40	11964.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.179098022958165	29.1684550340497	28.774978058325136	22.877468884667003
2	22.147623309455785	29.708727541163597	30.047542596296566	18.096106553084052
3	21.342354777469836	28.21149294887911	30.393817038218852	20.052335235432203
4	21.182747817845232	27.259689312244824	30.69394920404535	20.863613665864598
5	23.865658829834164	27.510917836987748	29.03319027849256	19.59023305468553
6	21.659778680316304	27.21636200960212	30.82003607891296	20.303823231168618
7	23.314292313725986	27.824914507294867	29.01757158241895	19.84322159656019
8	21.370901343998845	27.50453554570677	30.84702104277015	20.27754206752423
9	21.436394629455915	27.336011387193587	30.976213866224978	20.25138011712552
10	21.550689732984832	28.14485935647807	30.135641833227815	20.16880907730928
11	23.273028938577664	27.503514097562988	28.938965066960204	20.284491896899144
12	21.184145085682992	29.487960438384015	28.91017192270041	20.417722553232583
13	22.428017436972926	27.7644110296012	29.264538134965402	20.543033398460473
14	22.496680521002276	27.95617589714873	28.89165990612016	20.65548367572884
15	22.125117183825335	28.408220150247594	28.661176822958623	20.805485842968448
16	22.275970250941203	27.965688725945054	28.814396403763165	20.943944619350578
17	22.172803710358608	28.03443224753407	28.882917844086535	20.909846198020787
18	22.077573220140394	27.99576368729278	28.865762584255243	21.060900508311583
19	21.89270233795084	28.130709695915655	29.00721714123074	20.969370824902764
20	21.795040666477224	28.1683534676107	28.988760026070082	21.047845839841994
21	21.829996129886243	28.187173839292512	28.907033019414097	21.07579701140715
22	21.7846828575169	28.245368825429175	28.934324555922565	21.03562376113137
23	21.78732656896486	28.270002989658337	28.86172384294412	21.080946598432675
24	21.737269751736225	28.298826598306466	28.803161809593103	21.16074184036421
25	21.76749511560831	28.411452811518128	28.705701309073746	21.11535076379982
26	21.755920311291312	28.40043415248139	28.725584386552338	21.118061149674954
27	21.781121347319857	28.319916378221844	28.641184401564757	21.257777872893545
28	21.77021439553075	28.323268138019586	28.663390569299825	21.243126897149843
29	21.780991509580556	28.383636787138332	28.638259662138623	21.19711204114249
30	21.78064108502908	28.34488906086844	28.575276344831902	21.299193509270577
31	21.8806261593187	28.337383112571572	28.507833076406847	21.27415765170288
32	21.873279272600364	28.37730484215592	28.46574868092686	21.283667204316853
33	21.842765463066655	28.358729551418122	28.44078171465086	21.357723270864366
34	21.901778097928933	28.36287545057314	28.382197800830784	21.35314865066714
35	21.836324653492696	28.377314886598594	28.423136642043296	21.363223817865418
36	21.872283382154023	28.352007440396548	28.40457408729869	21.371135090150737
37	21.879933793563463	28.334592637745104	28.39074867581493	21.3947248928765
38	21.872652244533107	28.37094189312544	28.413337838259334	21.343068024082122
39	21.851041485872315	28.281072940314527	28.411607681155772	21.456277892657383
40	21.889289950741976	28.307035838691068	28.369823671662093	21.43385053890486
41	21.87156225476873	28.331361341477322	28.377174656276182	21.419901747477766
42	21.849964289188076	28.272091597083243	28.36408874238176	21.51385537134692
43	21.882238810307882	28.339046392594526	28.32100942153501	21.457705375562586
44	21.91889303732011	28.345076372944217	28.270958903492264	21.465071686243405
45	21.906857224840092	28.30673487867759	28.312002360408577	21.474405536073736
46	21.86281299238586	28.342461081100804	28.2872845780187	21.507441348494634
47	21.8953734072901	28.33925584648226	28.268007172561855	21.497363573665787
48	21.863247950330507	28.32566395523333	28.26453193906653	21.546556155369633
49	21.865596356211885	28.38543350468148	28.221831689244205	21.52713844986243
50	21.85860866622644	28.354867939710342	28.2735104572497	21.513012936813514
51	21.901250018140175	28.30817550027902	28.283031638540606	21.5075428430402
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.05656690762517
2	40.24372986253984
3	41.39469001290204
4	42.04636148370982
5	43.455891884519694
6	41.96360191148492
7	43.157513910286184
8	41.648443411523075
9	41.68777474658143
10	41.71949881029411
11	43.55752083547681
12	41.60186763891557
13	42.9710508354334
14	43.152164196731114
15	42.93060302679378
16	43.21991487029178
17	43.082649908379395
18	43.13847372845198
19	42.862073162853605
20	42.842886506319225
21	42.905793141293394
22	42.82030661864827
23	42.86827316739753
24	42.89801159210043
25	42.88284587940813
26	42.87398146096627
27	43.0388992202134
28	43.01334129268059
29	42.97810355072305
30	43.07983459429966
31	43.15478381102158
32	43.15694647691722
33	43.20048873393102
34	43.25492674859608
35	43.19954847135811
36	43.24341847230476
37	43.27465868643996
38	43.21572026861523
39	43.307319378529705
40	43.32314048964684
41	43.29146400224649
42	43.363819660535
43	43.339944185870465
44	43.383964723563516
45	43.38126276091383
46	43.370254340880486
47	43.39273698095589
48	43.40980410570014
49	43.392734806074316
50	43.371621603039955
51	43.40879286118037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109858.0
1	57661.0
2	5464.0
3	5955.5
4	6447.0
5	9599.5
6	12752.0
7	15822.5
8	18893.0
9	16754.5
10	14616.0
11	16770.0
12	18924.0
13	25218.0
14	31512.0
15	45409.5
16	59307.0
17	87541.0
18	115775.0
19	146962.5
20	178150.0
21	212373.0
22	246596.0
23	293614.5
24	340633.0
25	406681.0
26	558179.0
27	643629.0
28	705794.0
29	767959.0
30	823125.5
31	878292.0
32	949084.5
33	1019877.0
34	1088613.0
35	1157349.0
36	1264275.0
37	1371201.0
38	1426810.5
39	1482420.0
40	1534664.5
41	1586909.0
42	1591175.0
43	1595441.0
44	1590498.0
45	1585555.0
46	1542187.0
47	1498819.0
48	1446521.5
49	1394224.0
50	1318823.0
51	1243422.0
52	1152770.0
53	1062118.0
54	972130.0
55	882142.0
56	792354.0
57	702566.0
58	620783.0
59	539000.0
60	467833.0
61	396666.0
62	335204.0
63	273742.0
64	229379.0
65	185016.0
66	153435.5
67	121855.0
68	104004.0
69	86153.0
70	73720.5
71	61288.0
72	51313.0
73	41338.0
74	34161.5
75	22502.5
76	18020.0
77	16181.0
78	14342.0
79	14203.0
80	14064.0
81	11999.0
82	9934.0
83	8531.5
84	7129.0
85	5982.0
86	4835.0
87	5131.0
88	5427.0
89	3580.0
90	1733.0
91	1095.5
92	458.0
93	339.0
94	220.0
95	158.0
96	96.0
97	81.5
98	67.0
99	56.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8196040945156309
2	0.5203793547434044
3	0.5072653306795736
4	0.507247403100539
5	0.49412889714194946
6	0.5257710741380689
7	0.4973782708419759
8	0.49117981039075315
9	0.48764807732093124
10	0.5131276490238971
11	0.5057190769878368
12	0.49421853503712265
13	0.5103668018525643
14	0.4748433039954478
15	0.5203300539010591
16	0.47558729852538495
17	0.5081706734208223
18	0.49150698870813503
19	0.5109628938554658
20	0.5080855174204078
21	0.5210740484309963
22	0.5462847064484426
23	0.4875674032152754
24	0.5191916526323602
25	0.528083731833536
26	0.5368816912447799
27	0.5235615000220509
28	0.5193037000013266
29	0.5781151230244256
30	0.5758159110132345
31	0.5647590766436273
32	0.5755783705910257
33	0.5706527682512614
34	0.5613573185218066
35	0.5648263050650072
36	0.5939810304700719
37	0.5549347633326509
38	0.5643064052730029
39	0.5558849250214862
40	0.5654582522259779
41	0.5660722718079139
42	0.5621819871573994
43	0.5319426432207398
44	0.5447384527567058
45	0.5343494207061386
46	0.5277968905689819
47	0.5787156969220856
48	0.5848379651624113
49	0.5994713515494269
50	0.5865590127497357
51	0.5542938523821629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.2311992E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.022385553855912
#Duplication Level	Relative count
1	100.0
2	10.465882938359147
3	3.0540523729551534
4	1.435591217266903
5	0.9155937052932761
6	0.620140573490079
7	0.4322945823225726
8	0.4018162592523481
9	0.3253094482801518
10++	6.750637556758102
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	101198	0.4535587857865851	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	3659185	3.0418158	19.85675	4
TGTTT	3602625	2.2702718	15.250514	6
GTTTG	2566135	2.1331825	19.155317	7
GAGGG	1370525	2.028136	6.782182	11
CTTTG	2330040	2.0181515	20.089933	1
TTTGA	2926315	1.8865225	15.1281185	8
GTGTT	2268580	1.8858304	19.068975	5
TTGTG	2177370	1.810009	19.075014	3
TTTGT	2847270	1.7942685	14.864543	2
TGAGG	1597290	1.7918605	7.9274297	10
TTGAG	1886665	1.6044472	12.076051	9
ACTTT	1918625	1.2887622	5.4572496	3
AACTT	1874425	1.2880505	5.7884164	2
TAACT	1311820	0.90144473	5.497137	1
>>END_MODULE