FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_5_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_5_GCCAAT_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 22311992
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 3300630 2.7932193 19.73778 4
TGTTT 3258235 2.0680914 14.955011 6
GAGGG 1271665 1.9512618 6.6884236 11
CTTTG 2163330 1.8864697 19.534573 1
GTTTG 2200225 1.8619812 18.918804 7
TGAGG 1496140 1.7218454 7.965765 10
TTTGT 2689820 1.7073027 14.607577 2
TTTGA 2582700 1.6720687 14.730729 8
GTGTT 1913535 1.6193645 18.858316 5
TTGTG 1854345 1.5692736 18.852324 3
TTGAG 1675945 1.4466408 11.969471 9
Produced by FastQC (version 0.10.1)