##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_5_GCCAAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22311992	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89172410065403	34.0	31.0	34.0	31.0	34.0
2	33.10367362089409	34.0	33.0	34.0	31.0	34.0
3	33.15899575439073	34.0	33.0	34.0	31.0	34.0
4	36.48908335929844	37.0	37.0	37.0	35.0	37.0
5	36.43342293238542	37.0	37.0	37.0	35.0	37.0
6	36.40809180103686	37.0	37.0	37.0	35.0	37.0
7	36.400939369286256	37.0	37.0	37.0	35.0	37.0
8	36.39560681090241	37.0	37.0	37.0	35.0	37.0
9	38.20981067042333	39.0	39.0	39.0	37.0	39.0
10	38.19587753527341	39.0	39.0	39.0	37.0	39.0
11	38.18446116330626	39.0	39.0	39.0	37.0	39.0
12	38.1467229819731	39.0	39.0	39.0	37.0	39.0
13	38.144333280506736	39.0	39.0	39.0	37.0	39.0
14	39.70247743903816	41.0	40.0	41.0	37.0	41.0
15	39.65956056276822	41.0	40.0	41.0	37.0	41.0
16	39.64684883357793	41.0	40.0	41.0	37.0	41.0
17	39.63908758124331	41.0	40.0	41.0	37.0	41.0
18	39.6152439459462	41.0	40.0	41.0	37.0	41.0
19	39.604879160946275	41.0	40.0	41.0	37.0	41.0
20	39.5491221492012	41.0	40.0	41.0	37.0	41.0
21	39.540801377124914	41.0	40.0	41.0	37.0	41.0
22	39.509123120875984	41.0	39.0	41.0	37.0	41.0
23	39.46205547223215	41.0	39.0	41.0	37.0	41.0
24	39.45901441700051	41.0	39.0	41.0	37.0	41.0
25	39.41583526024929	41.0	39.0	41.0	37.0	41.0
26	39.35308523775017	41.0	39.0	41.0	36.0	41.0
27	39.27807252709664	41.0	39.0	41.0	36.0	41.0
28	39.23238368855636	40.0	39.0	41.0	36.0	41.0
29	39.173262208053856	40.0	39.0	41.0	36.0	41.0
30	39.126734538090545	40.0	39.0	41.0	36.0	41.0
31	39.070427284125955	40.0	39.0	41.0	36.0	41.0
32	39.018082069946956	40.0	39.0	41.0	36.0	41.0
33	38.94167168041294	40.0	38.0	41.0	35.0	41.0
34	35.73891891857975	40.0	38.0	41.0	24.0	41.0
35	38.809342796465685	40.0	38.0	41.0	35.0	41.0
36	38.75573453952475	40.0	38.0	41.0	35.0	41.0
37	38.90299319755941	40.0	38.0	41.0	35.0	41.0
38	38.93337277998307	40.0	39.0	41.0	35.0	41.0
39	38.913968326987565	40.0	39.0	41.0	35.0	41.0
40	38.87735828338411	40.0	38.0	41.0	35.0	41.0
41	38.84054341719018	40.0	38.0	41.0	35.0	41.0
42	38.78804160560832	40.0	38.0	41.0	35.0	41.0
43	38.74551209950236	40.0	38.0	41.0	35.0	41.0
44	38.69715137940172	40.0	38.0	41.0	35.0	41.0
45	38.642317996528504	40.0	38.0	41.0	35.0	41.0
46	38.5719862663988	40.0	38.0	41.0	35.0	41.0
47	38.51977295438256	40.0	38.0	41.0	35.0	41.0
48	38.46239224180432	40.0	38.0	41.0	34.0	41.0
49	38.42087371669908	40.0	38.0	41.0	34.0	41.0
50	38.539096688453455	40.0	38.0	41.0	35.0	41.0
51	38.25938329486672	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	3.0
9	2.0
10	6.0
11	11.0
12	27.0
13	67.0
14	122.0
15	237.0
16	384.0
17	606.0
18	1028.0
19	1888.0
20	3121.0
21	5128.0
22	8951.0
23	13675.0
24	20644.0
25	29934.0
26	41898.0
27	57307.0
28	76970.0
29	102972.0
30	134984.0
31	178043.0
32	235090.0
33	318836.0
34	457306.0
35	680957.0
36	1063558.0
37	1876808.0
38	4794718.0
39	1.2199311E7
40	7397.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.54434274925396	29.324918066057858	28.256821956238205	22.873917228449976
2	22.047113861591612	28.70037196569342	30.739670540573467	18.5128436321415
3	20.370237238107258	27.55697708940496	31.75333855875806	20.31944711372972
4	20.986895829056856	27.55280795339544	31.183171551785183	20.277124665762518
5	23.381717197359052	27.977552332414724	29.032631073955596	19.60809939627063
6	21.787597449837737	27.732104780245532	30.117575337961753	20.362722431954978
7	23.283299850591558	28.440723714852535	28.287348794316525	19.988627640239383
8	20.966313282515063	27.88071114506227	30.60638655139787	20.546589021024804
9	20.805829199412386	27.72777044264488	30.992666754482094	20.47373360346064
10	21.13548624434788	27.99932879144094	30.47178844452795	20.393396519683225
11	22.92119412735537	27.917247370830893	28.723948090336354	20.43761041147738
12	21.079216055652942	29.83223550815185	28.534709047941575	20.553839388253635
13	22.177307162892493	28.18513022055583	28.97461598229329	20.662946634258383
14	21.819854740562818	28.306573175983285	29.091539584379877	20.78203249907402
15	21.86615161927272	28.29657253372984	28.952152725762897	20.885123121234535
16	21.856018055223398	28.186152092560807	28.956437417152177	21.00139243506362
17	21.880785005659735	28.260865278187623	28.900319612879027	20.95803010327361
18	21.752284601034276	28.194206057442116	28.969394574899454	21.084114766624154
19	21.650704616487467	28.35296391877912	29.008598625354793	20.987732839378616
20	21.526818403305274	28.37671777580415	29.061076214082544	21.035387606808033
21	21.603617766457056	28.32995576169977	28.956124197109627	21.11030227473354
22	21.58727019981004	28.37062688082714	28.95050787038647	21.09159504897635
23	21.64658807694087	28.410874295759875	28.851435586746355	21.0911020405529
24	21.577540006288995	28.398674578226814	28.826700009573326	21.197085405910865
25	21.610014162435096	28.480626049638847	28.756309261974394	21.15305052595167
26	21.64366588155822	28.46804982719607	28.71978889200032	21.168495399245394
27	21.66633978714227	28.40269035593057	28.646774344487035	21.284195512440128
28	21.66401629404359	28.407974253446255	28.682463846627172	21.245545605882977
29	21.67507500002689	28.47851505145753	28.642588254782453	21.203821693733126
30	21.65718775804509	28.412384694293547	28.61666497549838	21.313762572162986
31	21.753124665258454	28.412790611684567	28.52892547908098	21.305159243975996
32	21.743026617071155	28.4824649815953	28.509513575301494	21.264994826032048
33	21.72772397019009	28.423744467584555	28.46372311755126	21.384808444674096
34	21.781780134659606	28.425444431303497	28.449352792055294	21.3434226419816
35	21.73397675878426	28.44916552727054	28.43791088573939	21.37894682820581
36	21.76432481721971	28.42139242866003	28.41704918785207	21.397233566268195
37	21.78224584833278	28.450353651369092	28.397882004636525	21.369518495661598
38	21.746008695913297	28.47883816646296	28.434278445815032	21.34087469180871
39	21.714800321931353	28.358867671863266	28.496769100578177	21.429562905627204
40	21.750217739503594	28.40838966711762	28.428460426629588	21.412932166749197
41	21.750812710281448	28.381368680665496	28.446038600981975	21.421780008071078
42	21.744860453410165	28.357082725979126	28.400086608340047	21.49797021227066
43	21.781437618905283	28.40701130110857	28.350591589007767	21.460959490978382
44	21.793615981555085	28.409697350093925	28.332405789789995	21.464280878561
45	21.807064303950693	28.350359658446834	28.36520387218845	21.47737216541402
46	21.74370820352644	28.40948745495135	28.368061297983733	21.478743043538472
47	21.77658106953817	28.449924689311473	28.322363092805553	21.4511311483448
48	21.77227833355265	28.370591025669068	28.34219822237297	21.514932418405312
49	21.812174793063118	28.43415051841507	28.269703571054812	21.483971117467004
50	21.755503076649333	28.386100330724677	28.32171232824525	21.536684264380742
51	21.79072581237928	28.353385031690582	28.31413707928902	21.541752076641117
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.41825997770394
2	40.55995749373311
3	40.68968435183698
4	41.26402049481938
5	42.98981659362968
6	42.15031988179271
7	43.27192749083094
8	41.51290230353986
9	41.27956280287302
10	41.52888276403111
11	43.35880453883275
12	41.63305544390658
13	42.84025379715088
14	42.60188723963684
15	42.751274740507256
16	42.857410490287016
17	42.838815108933346
18	42.83639936765844
19	42.63843745586608
20	42.56220601011331
21	42.7139200411906
22	42.678865248786394
23	42.73769011749376
24	42.77462541219986
25	42.76306468838676
26	42.812161280803615
27	42.95053529958239
28	42.90956189992657
29	42.87889669376002
30	42.97095033020808
31	43.058283909234454
32	43.0080214431032
33	43.112532414864184
34	43.12520277664121
35	43.112923586990064
36	43.1615583834879
37	43.15176434399438
38	43.08688338772201
39	43.14436322755856
40	43.16314990625279
41	43.172592718352526
42	43.24283066568083
43	43.24239710988366
44	43.25789686011608
45	43.28443646936472
46	43.22245124706492
47	43.22771221788297
48	43.28721075195796
49	43.29614591053012
50	43.29218734103007
51	43.332477889020396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9246.0
1	5843.0
2	2440.0
3	3679.5
4	4919.0
5	6280.5
6	7642.0
7	11532.0
8	15422.0
9	14081.0
10	12740.0
11	15461.0
12	18182.0
13	25260.5
14	32339.0
15	48084.5
16	63830.0
17	95530.5
18	127231.0
19	160403.0
20	193575.0
21	230950.5
22	268326.0
23	316713.0
24	365100.0
25	436002.5
26	595774.0
27	684643.0
28	742308.5
29	799974.0
30	852492.0
31	905010.0
32	972437.0
33	1039864.0
34	1105332.0
35	1170800.0
36	1271791.0
37	1372782.0
38	1424588.0
39	1476394.0
40	1525373.5
41	1574353.0
42	1575531.0
43	1576709.0
44	1570461.0
45	1564213.0
46	1523524.0
47	1482835.0
48	1430801.5
49	1378768.0
50	1305444.0
51	1232120.0
52	1144845.0
53	1057570.0
54	967495.5
55	877421.0
56	787162.0
57	696903.0
58	616475.0
59	536047.0
60	464683.5
61	393320.0
62	332559.5
63	271799.0
64	227763.5
65	183728.0
66	152411.5
67	121095.0
68	103432.5
69	85770.0
70	73205.0
71	60640.0
72	50524.5
73	40409.0
74	33390.5
75	21878.0
76	17384.0
77	15802.0
78	14220.0
79	14135.0
80	14050.0
81	11965.5
82	9881.0
83	8338.5
84	6796.0
85	5869.0
86	4942.0
87	5200.0
88	5458.0
89	3476.0
90	1494.0
91	865.5
92	237.0
93	153.0
94	69.0
95	44.0
96	19.0
97	11.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.2712890897415166
2	0.008381143198688849
3	0.0025457162229172545
4	5.378273710388566E-5
5	3.585515806925711E-5
6	0.0
7	0.0
8	9.008608464900847E-4
9	1.9272147462225697E-4
10	0.0
11	0.0
12	0.0
13	0.0
14	0.002079599168016912
15	0.0
16	0.0
17	0.0
18	0.0
19	0.002846003171747283
20	0.0
21	4.661170549003424E-4
22	0.0
23	0.0
24	0.0
25	6.767661085572279E-4
26	0.0
27	0.0
28	1.5686631655299985E-4
29	0.0
30	0.0
31	8.963789517314277E-6
32	0.001326640848562513
33	3.719972649685425E-4
34	8.481344919808146
35	0.005364828026112594
36	0.006557012031915393
37	0.004011295808998138
38	0.001622445902633884
39	0.0018062035877388267
40	0.004159198336033824
41	0.0010218720049738274
42	2.3754042220882834E-4
43	0.002872894540299226
44	0.0016627829554617982
45	0.001201147795320113
46	8.963789517314277E-6
47	0.0018734320091186836
48	0.0
49	0.0010622090578017418
50	0.0017120837978070267
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.2311992E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	13.360187982544478
#Duplication Level	Relative count
1	100.0
2	3.083968315467734
3	0.9196478539756339
4	0.49991390072753883
5	0.3427827284859443
6	0.22923931292780575
7	0.18296095397993886
8	0.14314004046665807
9	0.11246717465237419
10++	2.1099702957510007
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	3300630	2.7932193	19.73778	4
TGTTT	3258235	2.0680914	14.955011	6
GAGGG	1271665	1.9512618	6.6884236	11
CTTTG	2163330	1.8864697	19.534573	1
GTTTG	2200225	1.8619812	18.918804	7
TGAGG	1496140	1.7218454	7.965765	10
TTTGT	2689820	1.7073027	14.607577	2
TTTGA	2582700	1.6720687	14.730729	8
GTGTT	1913535	1.6193645	18.858316	5
TTGTG	1854345	1.5692736	18.852324	3
TTGAG	1675945	1.4466408	11.969471	9
>>END_MODULE