FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_1_AGTTCC_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_1_AGTTCC_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 30072319
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 138141 0.45936264509564423 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9708335 3.4326968 4.2023993 16
TGTGT 4899090 2.9779751 21.841208 4
TGTTT 5059880 2.3457859 16.99932 6
GTTTG 3699215 2.2486153 21.356474 7
GAGGG 1917845 2.0640283 7.342518 11
CTTTG 3156375 2.0107887 22.449322 1
TTTGA 4181475 1.9964539 17.025068 8
GTGTT 3218600 1.9564674 21.23314 5
TTGTG 2993315 1.819525 21.220482 3
TTTGT 3884290 1.8007765 16.611742 2
TGAGG 2165145 1.7771811 8.580335 10
TTGAG 2674380 1.6742117 13.454996 9
AACTT 2815005 1.4506507 6.79009 2
ACTTT 2619680 1.3108419 6.216468 3
TAACT 1887630 0.9727484 6.416017 1
Produced by FastQC (version 0.10.1)