##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_1_AGTTCC_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	30072319	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44149312196376	34.0	31.0	34.0	31.0	34.0
2	32.59980099971671	34.0	31.0	34.0	31.0	34.0
3	32.67244125735697	34.0	31.0	34.0	31.0	34.0
4	36.03115360009316	37.0	37.0	37.0	35.0	37.0
5	36.0455447749141	37.0	37.0	37.0	35.0	37.0
6	36.0334950889554	37.0	37.0	37.0	35.0	37.0
7	36.05450211538392	37.0	37.0	37.0	35.0	37.0
8	36.050258079531545	37.0	37.0	37.0	35.0	37.0
9	37.852117224481425	39.0	39.0	39.0	37.0	39.0
10	37.830274346318284	39.0	39.0	39.0	37.0	39.0
11	37.828158613241634	39.0	39.0	39.0	37.0	39.0
12	37.80108664050817	39.0	39.0	39.0	37.0	39.0
13	37.77969045220623	39.0	39.0	39.0	37.0	39.0
14	39.32747185210425	41.0	40.0	41.0	37.0	41.0
15	39.30591824328546	41.0	40.0	41.0	37.0	41.0
16	39.29311111657202	41.0	40.0	41.0	37.0	41.0
17	39.272186092465965	41.0	40.0	41.0	37.0	41.0
18	39.24924748902803	41.0	40.0	41.0	37.0	41.0
19	39.26326742543533	41.0	40.0	41.0	37.0	41.0
20	39.240297863294145	41.0	40.0	41.0	37.0	41.0
21	39.205738772590166	41.0	40.0	41.0	37.0	41.0
22	39.17161034371842	41.0	40.0	41.0	37.0	41.0
23	39.15203815841406	41.0	40.0	41.0	36.0	41.0
24	39.128253494517665	41.0	40.0	41.0	36.0	41.0
25	39.07566676184833	41.0	39.0	41.0	36.0	41.0
26	39.041942558536974	41.0	39.0	41.0	36.0	41.0
27	39.00431183907034	41.0	39.0	41.0	36.0	41.0
28	38.97450761944897	41.0	39.0	41.0	36.0	41.0
29	38.920442849784884	41.0	39.0	41.0	36.0	41.0
30	38.87962587787128	41.0	39.0	41.0	36.0	41.0
31	38.84299877239265	41.0	39.0	41.0	35.0	41.0
32	38.79929156777035	41.0	39.0	41.0	35.0	41.0
33	38.75655811578748	41.0	39.0	41.0	35.0	41.0
34	38.7190405568656	41.0	39.0	41.0	35.0	41.0
35	38.675461177436965	41.0	39.0	41.0	35.0	41.0
36	38.63444571733893	40.0	39.0	41.0	35.0	41.0
37	38.59982337910156	40.0	39.0	41.0	35.0	41.0
38	38.56177982150296	40.0	39.0	41.0	35.0	41.0
39	38.52740668253752	40.0	39.0	41.0	35.0	41.0
40	38.46824071000311	40.0	38.0	41.0	35.0	41.0
41	38.42031148312839	40.0	38.0	41.0	34.0	41.0
42	38.364441165977254	40.0	38.0	41.0	34.0	41.0
43	38.337637147304804	40.0	38.0	41.0	34.0	41.0
44	38.28071922221894	40.0	38.0	41.0	34.0	41.0
45	38.23364815995733	40.0	38.0	41.0	34.0	41.0
46	38.17936495020554	40.0	38.0	41.0	34.0	41.0
47	38.111108757525486	40.0	38.0	41.0	33.0	41.0
48	38.26398782215632	40.0	38.0	41.0	34.0	41.0
49	38.2934497336238	40.0	38.0	41.0	34.0	41.0
50	38.264458088516555	40.0	38.0	41.0	34.0	41.0
51	36.75930728854	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	144724.0
3	3382.0
4	2611.0
5	1696.0
6	2029.0
7	2365.0
8	5380.0
9	7706.0
10	8748.0
11	10991.0
12	14777.0
13	19170.0
14	23698.0
15	26489.0
16	27508.0
17	28613.0
18	30252.0
19	31208.0
20	33988.0
21	37256.0
22	41645.0
23	47754.0
24	55342.0
25	64187.0
26	75420.0
27	89606.0
28	109090.0
29	132648.0
30	164279.0
31	208491.0
32	273445.0
33	370173.0
34	532647.0
35	805518.0
36	1253660.0
37	2086666.0
38	4745897.0
39	1.8535901E7
40	17359.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.309993399761343	28.876456569128617	28.900843652940377	22.912706378169666
2	22.274775255885146	29.576679530582243	30.29283468101745	17.85571053251516
3	21.46709519503294	28.10623207281127	30.55664511156283	19.87002762059296
4	21.180797177660324	27.005753383604507	31.01218793194289	20.80126150679228
5	24.194817496956745	27.236695302091658	29.07334932339885	19.495137877552747
6	21.705259926690264	26.91134581902719	31.188167286986427	20.195226967296122
7	23.63741239961008	27.605306875712564	28.993283871234464	19.76399685344289
8	21.517516207366462	27.248871833215087	31.01638794867796	20.21722401074049
9	21.61689647762806	27.074064952160263	31.096084831273497	20.21295373893818
10	21.707377053810756	28.03071931999822	30.200409765198117	20.061493860992908
11	23.67949631114158	27.220442756598185	28.854971527621565	20.245089404638673
12	21.298373527293602	29.461209756730756	28.862072677483663	20.37834403849198
13	22.716568853671994	27.499415715879078	29.237864873400206	20.546150557048726
14	22.784305196435316	27.73150643205361	28.807433143267424	20.67675522824365
15	22.362203745222796	28.2162588502193	28.584391468488214	20.83714593606969
16	22.475799130974377	27.75147480376019	28.78449652779692	20.988229537468513
17	22.401906806117804	27.82653342813572	28.851339101518324	20.920220664228154
18	22.27002674617442	27.75515139233034	28.861241654519848	21.113580206975392
19	22.050926141338035	27.94043657587091	29.022222819433726	20.98641446335733
20	21.934293573843696	27.974063391810734	29.004640160830476	21.087002873515097
21	21.983043289021595	27.989898983145583	28.923026213302077	21.104031514530742
22	21.90115941734343	28.056484613016174	28.989934346407964	21.05242162323243
23	21.938796191948118	28.102537049284788	28.86041729179497	21.09824946697212
24	21.83303055268208	28.12639867862947	28.838958635132016	21.201612133556434
25	21.872425239528674	28.26061773757702	28.728871648254234	21.13808537464007
26	21.898520474473145	28.234051448244024	28.73549168131329	21.131936395969543
27	21.892474087402665	28.155632305886858	28.65072699157125	21.301166615139223
28	21.892810621994464	28.15996332466437	28.69893484679042	21.24829120655075
29	21.899870278600304	28.215919667593177	28.66296378778727	21.221246266019246
30	21.92498025717022	28.199775027067997	28.541415456369602	21.333829259392186
31	22.013770958247097	28.15958924512903	28.50765076832762	21.318989028296254
32	21.98500675548257	28.23243837098045	28.452937295327807	21.329617578209167
33	21.974903197905725	28.193057675217894	28.412285111391387	21.41975401548499
34	22.037199441376636	28.200664297477008	28.355543524888816	21.40659273625754
35	21.971773169859464	28.222990747430508	28.38683718254231	21.41839890016772
36	21.983332822598374	28.215666466967065	28.351601140709796	21.449399569724754
37	22.035941652199615	28.184129002890558	28.3506341615709	21.42929518333893
38	21.957887019284072	28.242359619746598	28.40933410304492	21.390419257924407
39	21.963513941517686	28.121816696353118	28.407064780890728	21.507604581238468
40	22.024682003513732	28.15760145622088	28.359150538710022	21.45856600155536
41	22.005856213245867	28.17972944603832	28.30958472564523	21.504829615070584
42	21.98223911006997	28.13700814582849	28.28799146204831	21.59276128205323
43	22.021943154442457	28.215499530998873	28.26038027739939	21.502177037159278
44	22.042157788343687	28.22751810604099	28.202607096026593	21.52771700958873
45	22.021507481600164	28.174241213024047	28.255625969540798	21.548625335834988
46	21.955501845219285	28.229668654861417	28.236174428551696	21.578655071367603
47	22.01331662127178	28.221429151531506	28.20141321102916	21.563841016167554
48	21.990138288418855	28.216440501157326	28.187070456829634	21.606350753594192
49	21.977427087581887	28.300305589752156	28.131302991464757	21.5909643312012
50	21.97525695284362	28.230829176285717	28.18754931622638	21.606364554644287
51	22.01900406322248	28.17456289969052	28.218664605822607	21.58776843126439
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.222699777931005
2	40.13048578840031
3	41.3371228156259
4	41.9820586844526
5	43.689955374509495
6	41.90048689398638
7	43.40140925305297
8	41.73474021810695
9	41.829850216566236
10	41.768870914803664
11	43.92458571578025
12	41.67671756578558
13	43.26271941072072
14	43.46106042467896
15	43.19934968129249
16	43.464028668442886
17	43.322127470345954
18	43.38360695314981
19	43.037340604695366
20	43.02129644735879
21	43.08707480355234
22	42.95358104057586
23	43.037045658920235
24	43.03464268623852
25	43.010510614168744
26	43.030456870442684
27	43.19364070254189
28	43.14110182854522
29	43.121116544619554
30	43.2588095165624
31	43.33275998654335
32	43.31462433369174
33	43.394657213390715
34	43.44379217763418
35	43.390172070027184
36	43.43273239232313
37	43.46523683553855
38	43.34830627720848
39	43.47111852275616
40	43.4832480050691
41	43.510685828316454
42	43.5750003921232
43	43.52412019160174
44	43.569874797932414
45	43.57013281743515
46	43.53415691658689
47	43.577157637439335
48	43.59648904201305
49	43.56839141878309
50	43.5816215074879
51	43.60677249448687
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154946.0
1	81825.0
2	8704.0
3	9910.0
4	11116.0
5	18436.0
6	25756.0
7	30462.5
8	35169.0
9	28913.5
10	22658.0
11	24619.0
12	26580.0
13	33986.0
14	41392.0
15	60032.0
16	78672.0
17	130596.0
18	182520.0
19	225258.0
20	267996.0
21	307545.5
22	347095.0
23	406441.5
24	465788.0
25	556829.5
26	773994.0
27	900117.0
28	973092.5
29	1046068.0
30	1095967.0
31	1145866.0
32	1231795.5
33	1317725.0
34	1399604.0
35	1481483.0
36	1656714.5
37	1831946.0
38	1894014.5
39	1956083.0
40	2026384.5
41	2096686.0
42	2088351.0
43	2080016.0
44	2073837.0
45	2067658.0
46	2009583.0
47	1951508.0
48	1889527.0
49	1827546.0
50	1746856.0
51	1666166.0
52	1557204.0
53	1448242.0
54	1337557.0
55	1226872.0
56	1112663.0
57	998454.0
58	891640.0
59	784826.0
60	684834.0
61	584842.0
62	497383.0
63	409924.0
64	346340.0
65	282756.0
66	234687.5
67	186619.0
68	159878.0
69	133137.0
70	113081.0
71	93025.0
72	78162.5
73	63300.0
74	53482.0
75	36570.0
76	29476.0
77	27114.0
78	24752.0
79	23419.5
80	22087.0
81	18977.5
82	15868.0
83	14339.0
84	12810.0
85	11224.5
86	9639.0
87	10842.5
88	12046.0
89	7678.0
90	3310.0
91	2079.5
92	849.0
93	611.0
94	373.0
95	276.5
96	180.0
97	139.0
98	98.0
99	68.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8285194101592233
2	0.526763499682216
3	0.5135087852719307
4	0.5135819422506126
5	0.5001509860280479
6	0.5322436224489371
7	0.5033266639662873
8	0.49766697407007426
9	0.4936400149253538
10	0.5192881865878052
11	0.5115967278745613
12	0.5003006253026246
13	0.5166445594036163
14	0.4809406284896086
15	0.5266338123109162
16	0.48143277543710544
17	0.5142503310103886
18	0.49725130941847223
19	0.5170968025445594
20	0.5141139930046632
21	0.5270594529141568
22	0.5528772157544617
23	0.4936832440491204
24	0.5251473955167874
25	0.5338996304209196
26	0.5429278666537156
27	0.530238456169609
28	0.525190624640554
29	0.585059635740097
30	0.5832240606386225
31	0.5714823655601685
32	0.5823328756255878
33	0.5775710213768349
34	0.5684829294342083
35	0.5711564844733125
36	0.6010677128025943
37	0.5611173518078203
38	0.5717616922060451
39	0.5627301306560362
40	0.5716552820552349
41	0.5723203454977982
42	0.5686358940259978
43	0.5385118453950957
44	0.5515504141865482
45	0.5405003850883598
46	0.5334706645004663
47	0.5857446510859372
48	0.5916204866009834
49	0.6060257607669033
50	0.5935923997081834
51	0.5610275682430743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.0072319E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.209782464224006
#Duplication Level	Relative count
1	100.0
2	14.682914153639956
3	4.700556626868529
4	2.3584841149359264
5	1.4476401482514805
6	0.9778580122813496
7	0.7480955702348226
8	0.6051778607475485
9	0.4820908382226234
10++	10.760541035845677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	138141	0.45936264509564423	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	9708335	3.4326968	4.2023993	16
TGTGT	4899090	2.9779751	21.841208	4
TGTTT	5059880	2.3457859	16.99932	6
GTTTG	3699215	2.2486153	21.356474	7
GAGGG	1917845	2.0640283	7.342518	11
CTTTG	3156375	2.0107887	22.449322	1
TTTGA	4181475	1.9964539	17.025068	8
GTGTT	3218600	1.9564674	21.23314	5
TTGTG	2993315	1.819525	21.220482	3
TTTGT	3884290	1.8007765	16.611742	2
TGAGG	2165145	1.7771811	8.580335	10
TTGAG	2674380	1.6742117	13.454996	9
AACTT	2815005	1.4506507	6.79009	2
ACTTT	2619680	1.3108419	6.216468	3
TAACT	1887630	0.9727484	6.416017	1
>>END_MODULE